##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n01 clinical flu pool e1.3410000000b607.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	512339
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77243582862128	34.0	34.0	34.0	34.0	34.0
2	33.85410050767168	34.0	34.0	34.0	34.0	34.0
3	33.860814812067794	34.0	34.0	34.0	34.0	34.0
4	33.85699702735884	34.0	34.0	34.0	34.0	34.0
5	33.87640800329469	34.0	34.0	34.0	34.0	34.0
6	37.65773638157548	38.0	38.0	38.0	38.0	38.0
7	37.696013772131344	38.0	38.0	38.0	38.0	38.0
8	37.63477111834157	38.0	38.0	38.0	38.0	38.0
9	37.5880618106371	38.0	38.0	38.0	37.0	38.0
10-14	37.42340286411926	38.0	38.0	38.0	37.0	38.0
15-19	37.47151046475088	38.0	38.0	38.0	37.0	38.0
20-24	37.53413774863908	38.0	38.0	38.0	37.0	38.0
25-29	37.46452407488011	38.0	38.0	38.0	37.0	38.0
30-34	37.37783967256055	38.0	38.0	38.0	37.0	38.0
35-39	37.4244845697868	38.0	38.0	38.0	37.0	38.0
40-44	37.31130560039348	38.0	38.0	38.0	37.0	38.0
45-49	37.393841577549246	38.0	38.0	38.0	37.0	38.0
50-54	37.41834527529624	38.0	38.0	38.0	37.0	38.0
55-59	37.31793909891693	38.0	38.0	38.0	37.0	38.0
60-64	37.33731064783278	38.0	38.0	38.0	37.0	38.0
65-69	37.309373286046934	38.0	38.0	38.0	37.0	38.0
70-74	37.28012780600345	38.0	38.0	38.0	37.0	38.0
75-79	36.70830602394118	38.0	38.0	38.0	36.0	38.0
80-84	36.562246481333645	38.0	38.0	38.0	35.6	38.0
85-89	36.58307800108912	38.0	38.0	38.0	36.0	38.0
90-94	36.35419165825751	38.0	38.0	38.0	34.2	38.0
95-99	36.18127724026475	38.0	38.0	38.0	33.6	38.0
100-104	36.50006187309574	38.0	38.0	38.0	35.2	38.0
105-109	36.46525679286566	38.0	38.0	38.0	35.0	38.0
110-114	36.339581800331416	38.0	38.0	38.0	34.8	38.0
115-119	36.27742646958362	38.0	38.0	38.0	34.0	38.0
120-124	36.23719997892021	38.0	38.0	38.0	34.2	38.0
125-129	36.2639619470702	38.0	38.0	38.0	34.0	38.0
130-134	36.28384526651299	38.0	38.0	38.0	34.0	38.0
135-139	36.22603939969434	38.0	38.0	38.0	34.0	38.0
140-144	36.17897290661066	38.0	38.0	38.0	34.0	38.0
145-149	36.128767085855266	38.0	38.0	38.0	34.0	38.0
150-154	36.0745834301117	38.0	38.0	38.0	34.0	38.0
155-159	36.03058638909003	38.0	38.0	38.0	33.6	38.0
160-164	35.99113946039633	38.0	37.6	38.0	33.2	38.0
165-169	35.89310983548002	38.0	37.2	38.0	32.2	38.0
170-174	35.7906163692399	38.0	37.0	38.0	31.8	38.0
175-179	35.64150064703253	38.0	37.0	38.0	31.0	38.0
180-184	35.51282920097826	38.0	37.0	38.0	29.6	38.0
185-189	35.275081537809925	38.0	37.0	38.0	28.0	38.0
190-194	35.130119705897854	38.0	37.0	38.0	27.0	38.0
195-199	34.83414692225265	38.0	36.4	38.0	24.6	38.0
200-204	34.74563521418436	38.0	36.0	38.0	24.0	38.0
205-209	34.4985847261286	38.0	35.8	38.0	23.0	38.0
210-214	34.26125553588542	38.0	35.4	38.0	22.0	38.0
215-219	33.281525318197524	38.0	34.2	38.0	17.2	38.0
220-224	33.20310263321746	38.0	34.0	38.0	18.0	38.0
225-229	32.7130380470743	37.4	32.6	38.0	16.0	38.0
230-234	32.299988093820694	37.0	30.4	38.0	14.8	38.0
235-239	31.995068889934203	37.0	29.2	38.0	14.4	38.0
240-244	31.16934373530026	37.0	26.4	38.0	11.2	38.0
245-249	30.20936762573218	36.4	24.0	38.0	10.0	38.0
250-254	29.302891249738945	35.0	21.6	37.0	9.6	37.8
255-259	28.707316444775824	34.4	20.6	37.0	9.0	37.8
260-264	27.457929613010133	32.6	17.4	37.0	9.0	37.2
265-269	26.38817228436641	31.0	15.8	37.0	8.8	37.0
270-274	25.61877272665169	29.2	14.8	37.0	8.2	37.0
275-279	24.75258490960087	27.6	13.6	36.8	8.0	37.0
280-284	23.380662022606124	24.8	12.4	35.0	8.0	37.0
285-289	22.26047870648145	22.6	10.4	33.0	8.0	37.0
290-294	21.385640367022617	21.0	9.6	31.6	8.0	37.0
295-299	20.11927766576427	18.8	8.4	30.2	7.4	36.8
300	14.501293089146055	12.0	8.0	19.0	7.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.23900553327067797
1101	2	-0.03883204090568171
1101	3	-0.015851775738170204
1101	4	-0.017412686087681095
1101	5	0.007981109029977063
1101	6	0.044667418415379245
1101	7	0.01755374509647112
1101	8	-0.05720386064126615
1101	9	0.015950112316531317
1101	10-14	0.23702742559912338
1101	15-19	0.10448048178557912
1101	20-24	0.041960222972853956
1101	25-29	0.005276762851458727
1101	30-34	0.11781285216093096
1101	35-39	-0.003069706130979455
1101	40-44	-0.03646481305576543
1101	45-49	-0.009477998614826788
1101	50-54	0.04641522132785525
1101	55-59	0.04568733462951968
1101	60-64	0.06153026279504559
1101	65-69	0.07419675303145112
1101	70-74	0.002565760673270745
1101	75-79	0.13909578820664592
1101	80-84	0.07113301985071985
1101	85-89	0.06860009010786428
1101	90-94	0.27169763340751274
1101	95-99	0.5328028056174148
1101	100-104	0.11104079394704769
1101	105-109	0.058297206530319556
1101	110-114	0.20208882243218085
1101	115-119	0.14414092251652022
1101	120-124	0.12648775438391624
1101	125-129	0.14619636196709962
1101	130-134	0.08586028135709256
1101	135-139	0.051742279064420416
1101	140-144	0.026326993800680043
1101	145-149	-0.03479624619255617
1101	150-154	0.047943111442073416
1101	155-159	-0.0576839444123749
1101	160-164	-0.0016468522635051386
1101	165-169	-0.015042751472691407
1101	170-174	-0.03382540513369747
1101	175-179	-0.0511418037870115
1101	180-184	-0.1002560342738974
1101	185-189	0.06321054776687163
1101	190-194	0.045841810375478076
1101	195-199	0.20207841483428268
1101	200-204	0.016302347409990148
1101	205-209	0.10956744359226178
1101	210-214	0.051404569064061434
1101	215-219	0.7560201956149726
1101	220-224	0.36554434026953686
1101	225-229	0.041011619650731745
1101	230-234	0.4345906685732075
1101	235-239	-0.45039901793026615
1101	240-244	-0.05528790963947117
1101	245-249	0.4098298418329236
1101	250-254	0.2105035677066205
1101	255-259	-0.2363398293882213
1101	260-264	-0.825107784529532
1101	265-269	-0.9210176094917628
1101	270-274	-1.189338445388067
1101	275-279	-1.2986167119451473
1101	280-284	-0.923399014953663
1101	285-289	-0.4572024947518365
1101	290-294	-0.5235181112249236
1101	295-299	0.07196281985312325
1101	300	-1.0436948943120008
1102	1	-0.07633807814585225
1102	2	0.0027812625337020336
1102	3	0.04076647664643929
1102	4	-0.03485416046225964
1102	5	-0.054768248766635
1102	6	0.03007145578492043
1102	7	0.04075370461947614
1102	8	0.016976032200098246
1102	9	0.043224650704850376
1102	10-14	0.22633791472777887
1102	15-19	0.2138207594535544
1102	20-24	0.02504073319382627
1102	25-29	0.134118309114875
1102	30-34	0.18522359985747272
1102	35-39	-0.028716626558896508
1102	40-44	-0.008851279825101699
1102	45-49	0.07257909713961652
1102	50-54	0.0652536791121392
1102	55-59	0.1379127999305041
1102	60-64	0.06980401377931145
1102	65-69	0.0948908281057399
1102	70-74	0.029066176511165054
1102	75-79	0.10905110539766838
1102	80-84	0.10337199856550683
1102	85-89	-0.04304358161948585
1102	90-94	0.3516142971754448
1102	95-99	0.4923367720362606
1102	100-104	0.08960674878778718
1102	105-109	-0.003538256588704769
1102	110-114	0.08723397568081737
1102	115-119	0.15999420183836577
1102	120-124	0.2412258081361074
1102	125-129	0.2408143972094905
1102	130-134	0.1664346963116543
1102	135-139	0.17457162109187152
1102	140-144	0.17407930831537044
1102	145-149	0.2687386393914153
1102	150-154	0.23979216176108764
1102	155-159	0.15324790139136013
1102	160-164	0.18644310786032747
1102	165-169	0.19034553781555275
1102	170-174	0.23095191341288768
1102	175-179	0.15746440669126827
1102	180-184	0.24637346523437031
1102	185-189	0.4044197136983101
1102	190-194	0.3641064224129593
1102	195-199	0.5966572379867046
1102	200-204	0.4248095789837336
1102	205-209	0.39518493387092946
1102	210-214	0.5314266416760063
1102	215-219	1.4106044789455154
1102	220-224	1.0834549894088212
1102	225-229	0.9467263601288849
1102	230-234	1.0204188711310564
1102	235-239	0.9668877162612972
1102	240-244	1.2064888407534404
1102	245-249	1.1863105989214056
1102	250-254	1.4133824940542965
1102	255-259	1.3497393516272211
1102	260-264	1.5733136126225347
1102	265-269	1.4024956251156446
1102	270-274	0.20213556026395096
1102	275-279	0.09036595567862449
1102	280-284	0.511291562655181
1102	285-289	0.6178272041899646
1102	290-294	0.7565120778680026
1102	295-299	0.9048294028251718
1102	300	-0.023227659070029105
1103	1	-0.16775123361464495
1103	2	-0.05684444606711736
1103	3	-0.011302908878292328
1103	4	0.012141872624546579
1103	5	0.007818045152014008
1103	6	0.0137022033248968
1103	7	-0.08920232980214848
1103	8	0.047003187410268765
1103	9	-0.04622799035833225
1103	10-14	0.26244016181099283
1103	15-19	0.1543014191875045
1103	20-24	0.0672197406729893
1103	25-29	0.08625151564046973
1103	30-34	0.09846916888636059
1103	35-39	0.09096923229240872
1103	40-44	0.04060686736364261
1103	45-49	0.07608654083740163
1103	50-54	0.08188530679794326
1103	55-59	0.12336912008667866
1103	60-64	0.06409036567912807
1103	65-69	0.059294560591460765
1103	70-74	0.09014183151067812
1103	75-79	0.14999060892868243
1103	80-84	0.18042396888248646
1103	85-89	0.05008710960783702
1103	90-94	0.3461156799637948
1103	95-99	0.41941710724252346
1103	100-104	0.2234644154419385
1103	105-109	0.07212133136957277
1103	110-114	0.29061774011876196
1103	115-119	0.3005687159853565
1103	120-124	0.32977702970342904
1103	125-129	0.1905626588171927
1103	130-134	0.23282021686994625
1103	135-139	0.18399877546777788
1103	140-144	0.2752107754058528
1103	145-149	0.2822968478983725
1103	150-154	0.3753744553960914
1103	155-159	0.22519127172418507
1103	160-164	0.15781792901552905
1103	165-169	0.2543117001711792
1103	170-174	0.19541133285034107
1103	175-179	0.2416941539517694
1103	180-184	0.16255709452195077
1103	185-189	0.42610613150478827
1103	190-194	0.35990591601625965
1103	195-199	0.6417749314206489
1103	200-204	0.474721981531566
1103	205-209	0.4698826183782856
1103	210-214	0.618366904384942
1103	215-219	1.340312529151369
1103	220-224	0.8509156644097615
1103	225-229	0.9789747364475403
1103	230-234	1.2415701102178716
1103	235-239	0.958457504317181
1103	240-244	1.229686523175129
1103	245-249	1.603707870052201
1103	250-254	1.0813449773169204
1103	255-259	1.1589233187906878
1103	260-264	1.2211620523450613
1103	265-269	1.1749756341256692
1103	270-274	0.6018884103096305
1103	275-279	-0.1252318135062609
1103	280-284	-0.03436798359001614
1103	285-289	0.2984410537642823
1103	290-294	0.40909620270688407
1103	295-299	0.6363801280069339
1103	300	-0.16822647024746296
2116	1	-0.036558352421764084
2116	2	-0.019021158243830882
2116	3	-0.0046521522275355665
2116	4	0.04355490403757756
2116	5	-0.09112005378608501
2116	6	-0.04400860438591536
2116	7	-0.11218860278842158
2116	8	-0.06893642852934789
2116	9	-0.05341524754558691
2116	10-14	0.2220953214661492
2116	15-19	0.10050521539130841
2116	20-24	0.03915151260475369
2116	25-29	0.0433592227481725
2116	30-34	0.05942302984021097
2116	35-39	-0.02697023564705603
2116	40-44	0.048093824850610645
2116	45-49	0.07359088834174798
2116	50-54	0.06127813619077216
2116	55-59	0.1285639252814761
2116	60-64	0.05500870659746937
2116	65-69	0.05262715392405681
2116	70-74	-0.03393594256709065
2116	75-79	0.12309343203151002
2116	80-84	0.15932284778136108
2116	85-89	0.07468843420916471
2116	90-94	0.29806373191183866
2116	95-99	0.38604862387403927
2116	100-104	0.03565222762975395
2116	105-109	-0.03183397258572995
2116	110-114	0.07488902439004619
2116	115-119	0.18898585440249605
2116	120-124	0.33323838316478316
2116	125-129	0.27758304583758786
2116	130-134	0.07516305921278388
2116	135-139	0.08284067430967923
2116	140-144	0.12955457974964446
2116	145-149	0.13905675465828438
2116	150-154	0.20913883916047382
2116	155-159	0.20422705075996106
2116	160-164	0.13412680532440646
2116	165-169	0.13165657751605409
2116	170-174	0.28691797435698874
2116	175-179	0.43890990116751283
2116	180-184	0.32375700572185906
2116	185-189	0.13370038909904736
2116	190-194	0.2051569612672992
2116	195-199	0.34087583052154713
2116	200-204	0.27699193380151854
2116	205-209	0.414808063303731
2116	210-214	0.5303345663525931
2116	215-219	1.143242932081769
2116	220-224	0.5871516506457439
2116	225-229	1.034907692380493
2116	230-234	0.9924840111317721
2116	235-239	0.6299711758308462
2116	240-244	0.8473521908407982
2116	245-249	1.7260484923928274
2116	250-254	1.7852133311852754
2116	255-259	0.9101609700283397
2116	260-264	1.241650822833833
2116	265-269	1.3174276265776577
2116	270-274	0.9430306014518166
2116	275-279	-0.16961705789150372
2116	280-284	0.942632793410759
2116	285-289	0.39622845155168207
2116	290-294	0.6206679642786455
2116	295-299	0.5104560520828585
2116	300	-0.21384751586851003
1117	1	0.002159899171395807
1117	2	0.049700861831517784
1117	3	0.03498921916219189
1117	4	-0.004727058655504379
1117	5	-0.015133413424905484
1117	6	-0.06991665553171345
1117	7	-0.07098653086213602
1117	8	0.03624908740964372
1117	9	0.07932769024490227
1117	10-14	0.2307095228382181
1117	15-19	0.10667169785624964
1117	20-24	0.05623888755606288
1117	25-29	0.027761612198105468
1117	30-34	0.09571520226991481
1117	35-39	-9.17778797237645E-4
1117	40-44	0.07330402065333885
1117	45-49	0.024254383994275486
1117	50-54	0.010765379825777188
1117	55-59	0.09618062947463102
1117	60-64	-0.08908063493152696
1117	65-69	0.005945054425566809
1117	70-74	0.15262429878465866
1117	75-79	0.09838770615533576
1117	80-84	0.139917275705713
1117	85-89	0.12879169906589993
1117	90-94	0.32304000736360905
1117	95-99	0.405119677180636
1117	100-104	-0.007356710112176756
1117	105-109	-0.10656012426872508
1117	110-114	0.11606681757954362
1117	115-119	0.19027885547603773
1117	120-124	0.23139879383434447
1117	125-129	0.1360141538663413
1117	130-134	0.0707469681105195
1117	135-139	0.2048884063062033
1117	140-144	0.19682578696233577
1117	145-149	0.22853603909002373
1117	150-154	0.33235670071044865
1117	155-159	0.26872182608136796
1117	160-164	0.21254733626870603
1117	165-169	0.1096965656511415
1117	170-174	0.15327703998452336
1117	175-179	0.22670666913984405
1117	180-184	0.03137176761606497
1117	185-189	-0.6829547187777152
1117	190-194	-0.18341018157928346
1117	195-199	-0.187970158895034
1117	200-204	0.14118575834647373
1117	205-209	0.17042991036668553
1117	210-214	0.3586792526343743
1117	215-219	0.9131515490276954
1117	220-224	0.7420988655051772
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2108	230-234	0.6718583011443613
2108	235-239	0.5363526194463333
2108	240-244	0.7343068437529006
2108	245-249	1.3700554310806154
2108	250-254	0.7600437017603312
2108	255-259	0.6299362302291343
2108	260-264	0.991483496070753
2108	265-269	1.146158706372571
2108	270-274	1.1382287838840455
2108	275-279	1.5517194070619809
2108	280-284	1.1491355105517727
2108	285-289	1.1016719442292136
2108	290-294	0.5603873465512166
2108	295-299	1.1818078681155235
2108	300	0.27578283014481464
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	13.0
15	29.0
16	25.0
17	184.0
18	2952.0
19	6085.0
20	5689.0
21	3927.0
22	2722.0
23	2330.0
24	2558.0
25	3541.0
26	5031.0
27	6984.0
28	9557.0
29	12873.0
30	17847.0
31	23431.0
32	31787.0
33	45682.0
34	54159.0
35	81633.0
36	129788.0
37	63505.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.82432725207333	26.934900524847805	13.304081867669648	23.936690355409212
2	38.78916108279869	26.117277818007217	21.446737414094965	13.646823685099124
3	38.228204372495554	20.688840787057007	25.297117728691354	15.785837111756084
4	38.96228864091939	20.0915799890307	20.590078053788606	20.356053316261303
5	32.272967703024754	21.183044819933677	30.476110543995283	16.067876933046286
6	31.907584626585134	21.128198321814267	30.568822595976492	16.395394455624107
7	16.86715241275796	46.027337368422074	20.935162070426028	16.170348148393934
8	27.59559588475599	24.643644149674337	30.960165046970857	16.80059491859882
9	19.96178311625701	20.910568978742592	31.1748666410326	27.9527812639678
10-14	24.897616617122647	34.35572931203754	22.992784074606853	17.753869996232964
15-19	22.635013145593057	32.09054942137921	23.022530004547768	22.25190742847997
20-24	23.185508032767366	31.544387602739594	26.520018971813585	18.750085392679456
25-29	19.80091306732456	25.990447730896925	35.45133202820789	18.757307173570624
30-34	19.77140135730444	28.629676835064284	29.900632198602878	21.6982896090284
35-39	21.838587341584383	29.100575985821887	27.622921542182034	21.4379151304117
40-44	19.872623399741187	29.06595047419775	29.50749406154909	21.553932064511976
45-49	20.926847263237818	29.144765477545143	27.85651687652121	22.07187038269583
50-54	22.26974717911383	28.04334630000839	28.253285422347314	21.433621098530466
55-59	20.65889967384876	25.560927432813042	31.2607472786573	22.519425614680905
60-64	21.05598051290259	26.05622449198675	31.014660215209073	21.873134779901587
65-69	20.746849254107143	26.992869955244476	30.23435654908176	22.02592424156662
70-74	20.722490382344503	27.701072922420504	28.461506931933737	23.11492976330125
75-79	20.21446737414095	27.022811068452725	30.728131178770308	22.03459037863602
80-84	22.98372757100279	26.62003087799289	27.217096492751867	23.179145058252445
85-89	20.806028820761256	29.094173974653504	26.29239624545467	23.807400959130575
90-94	19.84424375267157	29.965667263276853	28.807683974868205	21.382405009183373
95-99	21.557328253363494	28.90843757746336	26.805064615420648	22.729169553752495
100-104	21.473048118530894	29.13406943449552	27.271786844257417	22.12109560271617
105-109	23.247303055203684	28.582559594331098	24.457361239335672	23.712776111129543
110-114	21.188548988072352	28.715908802570173	27.946379252799414	22.14916295655806
115-119	21.305776058430062	30.588848399204434	25.761107391785515	22.34426815057999
120-124	19.927977374355653	29.228303915961895	28.217332664505335	22.626386045177117
125-129	20.69454013846301	26.999311003066328	29.782585358522383	22.523563499948278
130-134	23.54987615621688	28.45975028252778	26.87513540839171	21.11523815286363
135-139	22.7304187266634	29.419076041449117	25.523959721980955	22.326545509906527
140-144	21.299100790687415	29.554572265628813	26.88188874944129	22.264438194242484
145-149	20.26681552643855	28.738432951619924	28.97331649552347	22.021435026418054
150-154	22.298829485945827	27.648646696815977	26.4596683055555	23.592855511682696
155-159	20.62243943951173	28.41997193264616	29.054356588118413	21.9032320397237
160-164	22.902999771635578	28.804990445779065	26.99037160942267	21.30163817316269
165-169	19.74334171060244	26.911606148119173	28.633201311319773	24.71185082995861
170-174	19.978607913900756	29.637095751055455	26.74053702724173	23.64375930780206
175-179	20.276965056339648	29.79472575775024	27.157370412949238	22.77093877296087
180-184	20.188547036239676	29.43227043032055	26.200855293077435	24.17832724036234
185-189	22.72047324934204	27.775760883228052	26.93104640913396	22.572719458295957
190-194	20.430574287727463	30.358375997142517	26.723556082984118	22.487493632145902
195-199	20.384003560142798	30.31531856836977	25.794639877112612	23.506037994374818
200-204	21.79677908572254	28.664107163421093	26.810998186747447	22.72811556410892
205-209	20.83764851006853	29.28888880214077	27.65266747212295	22.220795215667753
210-214	21.816102229188097	28.498318496151963	26.908745967025737	22.776833307634202
215-219	22.026548828022072	27.685145967806474	27.09725396661195	23.191051237559506
220-224	18.9474547126024	28.244580248624445	27.403418439743998	25.40454659902916
225-229	20.702737835690822	29.32940884843824	26.31601342080146	23.651839895069475
230-234	19.867119231602512	28.72914222809507	27.152178538038292	24.251560002264124
235-239	20.823751461434714	28.22595976492127	25.36511957902873	25.585169194615283
240-244	20.785659801678108	30.420495187949847	25.155463533115196	23.638381477256846
245-249	20.521998130144297	29.41536755936987	25.191757800987236	24.870876509498594
250-254	19.218095831080593	31.320043955271803	24.81552253488413	24.646337678763473
255-259	19.28246727264565	30.988661804000866	25.771295958340083	23.9575749650134
260-264	19.067141092128452	31.657750044404192	25.261086897542445	24.014021965924908
265-269	18.8353414438487	30.920269587128836	25.52583348134731	24.718555487675154
270-274	18.10758111328632	31.927454283199207	24.88309498203338	25.08186962148109
275-279	17.76265324326276	31.78953778650464	24.05836760426202	26.38944136597058
280-284	16.264660703167237	32.34834748086716	25.697243426715517	25.689748389250088
285-289	18.55748578870076	30.828935852603784	25.637800611626528	24.975777747068932
290-294	17.487405799675606	32.60825351964227	25.312381060196472	24.591959620485653
295-299	17.048282484839138	32.8949387026949	23.818370258754456	26.238408553711505
300	15.443876027395923	36.37689108188133	23.146198122727334	25.03303476799541
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.25
20	0.0
21	0.5
22	0.25
23	0.75
24	2.0
25	1.5
26	3.5
27	5.0
28	6.25
29	11.25
30	20.0
31	35.75
32	72.25
33	123.75
34	215.5
35	361.0
36	621.5
37	1174.0
38	2679.75
39	5381.5
40	9385.0
41	14342.75
42	20962.25
43	25141.5
44	25320.75
45	17168.0
46	11895.0
47	9854.0
48	8579.75
49	7480.5
50	6487.0
51	2316.0
52	415.5
53	96.75
54	53.25
55	32.25
56	27.75
57	13.0
58	12.25
59	12.0
60	6.75
61	7.5
62	4.5
63	3.25
64	4.5
65	2.0
66	1.75
67	0.5
68	0.5
69	1.0
70	0.75
71	1.5
72	0.75
73	1.5
74	2.0
75	0.5
76	1.0
77	0.75
78	1.0
79	1.25
80	1.0
81	0.25
82	0.25
83	0.0
84	0.25
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.25
91	0.25
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	3.9036653465771685E-5
170-174	0.0
175-179	0.0
180-184	0.0
185-189	3.9036653465771685E-5
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	3.9036653465771685E-5
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	0.0
285-289	3.9036653465771685E-5
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	512339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.669943178348161
#Duplication Level	Percentage of deduplicated	Percentage of total
1	51.05147954194671	5.44716385883175
2	3.684770536717341	0.7863258450405096
3	2.706497401886681	0.8663452047143345
4	2.644206835845505	1.128541467610853
5	2.5598109743314184	1.365651882171414
6	2.638447715424737	1.689125232157468
7	2.7599956393575305	2.061429765110349
8	2.5912580997328742	2.2118861347667362
9	2.46356621749346	2.3657500401077987
>10	26.010008424008856	52.2019798201946
>50	0.7477290586903113	5.072101513042962
>100	0.09407327561472707	1.589887079364851
>500	0.003704329158481821	0.32450009912331124
>1k	0.03704329149285714	13.284741544326234
>5k	0.0018521645746428568	1.585939884163191
>10k+	0.005556493723928571	8.018630629273705
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	16127	3.1477205522125	TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	13573	2.6492224874545953	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	10875	2.1226180322013355	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	8025	1.566345720314089	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	4994	0.9747452370403189	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	4832	0.9431255477330438	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4784	0.9337567509012588	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4779	0.9327808345646144	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	4637	0.9050648106039165	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	4552	0.8884742328809635	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	4338	0.8467050136725879	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	4130	0.8061068940681853	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	3834	0.7483326469388432	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3663	0.7149563082256084	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	3278	0.6398107503039979	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	2911	0.568178491194307	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	2876	0.5613470768377968	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	2417	0.4717579571338508	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2126	0.4149596263411531	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2027	0.395636482875596	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	1940	0.3786555386179854	No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	1857	0.3624553274296901	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1728	0.3372766859442674	No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1519	0.29648338307253597	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	923	0.18015415574453633	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	719	0.1403367692094492	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.004489215148563744	0.0	0.0
2	0.008002513960483196	0.0	0.0
3	0.012296545841718081	0.0	0.0
4	0.018152043861583835	0.0	0.0
5	0.02225089247548986	0.0	0.0
6	0.02478827495076502	0.0	0.0
7	0.03513298811919452	0.0	0.0
8	0.04469696821830858	0.0	0.0
9	0.05601759772338237	0.0	0.0
10-14	0.10481341455559696	0.0	0.0
15-19	0.16516408081368	0.0	0.0
20-24	0.2254366737648315	0.0	0.0
25-29	0.273568867488128	0.0	0.0
30-34	0.3207251448747802	0.0	0.0
35-39	0.36284569396434785	0.0	0.0
40-44	0.3991888183409813	0.0	0.0
45-49	0.4343218064601758	0.0	0.0
50-54	0.4689082814308495	0.0	0.0
55-59	0.5082181914708815	0.0	0.0
60-64	0.5555306154713968	0.0	0.0
65-69	0.610923626739327	0.0	0.0
70-74	0.676895571096481	0.0	0.0
75-79	0.7532903019289962	0.0	0.0
80-84	0.8473686367815061	0.0	0.0
85-89	0.9530018210598843	0.0	0.0
90-94	1.0719074675166247	0.0	0.0
95-99	1.2184120279736657	0.0	0.0
100-104	1.38427876854973	0.0	0.0
105-109	1.5718498884527627	0.0	0.0
110-114	1.7867857024353018	0.0	0.0
115-119	2.0390015204776524	0.0	0.0
120-124	2.32697491309465	0.0	0.0
125-129	2.6642515990389173	0.0	0.0
130-134	3.04275099104304	0.0	0.0
135-139	3.460052816592139	0.0	0.0
140-144	3.9482061681816143	0.0	0.0
145-149	4.524348136682939	0.0	0.0
150-154	5.167555075838459	0.0	0.0
155-159	5.8874690390542215	0.0	0.0
160-164	6.704623306053219	0.0	0.0
165-169	7.628777040201898	0.0	0.0
170-174	8.685967689361927	0.0	0.0
175-179	9.850704318820156	0.0	0.0
180-184	11.82201628218816	0.0	0.0
185-189	15.192636125690218	0.0	0.0
190-194	16.658657646597273	0.0	0.0
195-199	18.293512693743793	0.0	0.0
200-204	20.025334788099286	0.0	0.0
205-209	21.799706834732472	0.0	0.0
210-214	23.636264270336632	0.0	0.0
215-219	25.57650305754588	0.0	0.0
220-224	27.62167236927113	0.0	0.0
225-229	29.688702206937204	0.0	0.0
230-234	31.810071066227636	0.0	0.0
235-239	33.9546667343302	0.0	0.0
240-244	35.96021384278768	0.0	0.0
245-249	37.95377669863119	0.0	0.0
250-254	39.955107848514366	0.0	0.0
255-259	41.91470881584263	0.0	0.0
260-264	43.86958634810155	0.0	0.0
265-269	45.80295468430082	0.0	0.0
270-274	47.65379953507346	0.0	0.0
275-279	49.35513400307219	0.0	0.0
280-284	50.99338523907023	0.0	0.0
285-288	52.426420787798705	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	5295	0.0	291.2238	1
GGGTTAT	5345	0.0	289.0496	2
GGGGAGC	6410	0.0	275.65366	3
GGGGGGA	6530	0.0	269.91272	1
GGGAGCA	2450	0.0	268.2	4
GGGAGCG	4165	0.0	266.47058	4
GTTATTA	5815	0.0	265.687	4
GGAGCGA	4145	0.0	264.2099	5
GGTTATT	5870	0.0	263.19763	3
TTAGTAG	5865	0.0	261.6675	8
TTATTAG	5950	0.0	259.90585	5
TATTAGT	6195	0.0	247.9661	6
GGGGGAG	7280	0.0	242.30768	2
ATTAGTA	6375	0.0	241.1953	7
GCGTAAG	55	0.0	240.54547	8
TAGTAGA	6485	0.0	236.65073	9
CGAAAGC	4635	0.0	234.05826	9
GGAGCGT	65	0.0	226.15384	5
GCGAAAG	4830	0.0	224.91306	8
GAGCGAA	4880	0.0	223.21106	6
>>END_MODULE