FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool d4.3410000000b763.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool d4.3410000000b763.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences854480
Sequences flagged as poor quality0
Sequence length300
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT799879.360897856005993TruSeq Adapter, Index 1 (97% over 36bp)
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAA118711.389265986330868No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTATTTTAAATTCACAAGCAC113361.3266548076022844No Hit
CCGGGTTATTAGTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAG93171.0903707518022656No Hit
GGGGGGAGCAGAAGCAGAGGATTTGTTTAGTCACTGGCAAACAGGAAAAA73060.8550229379271603No Hit
CCGGGTTATTAGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACAT54440.6371126299035671No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAA54070.6327825110008426No Hit
GGGGGGAGCAGAAGCGGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA51410.6016524669974721No Hit
GGGGGGAGCAGAAGCAGAGCATCTTCTCAAAACTGAAGCAAATAGGCCAA49880.5837468401835034No Hit
GGGGGGAGCAGAAGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAAT45470.5321365040726523No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTTTTCAGAAACAATTAAGTTTAG44540.5212526916955341No Hit
GGGGGGAGCAGAAGCAGAGCATTTTCTAATATCCACAAAATGAAGGCAAT34470.4034032393970602No Hit
CCGGGTTATTAGTAGAAACACGTGCCTTTTTTCATTTTAGTCATTTGTGT32110.375784102612115No Hit
GGGGGGAGCAGAAGCGGTGCGTTTGATTTGTCATAATGGATACTTTTATT31280.3660705926411385No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTCACTCAATTGCGTTCATTG28350.33178073214118525No Hit
CCGGGTTATTAGTAGAAACACGTGCATTTTTGATTCTTGGTTCTTTTTAT28320.3314296414193427No Hit
CCGGGTTATTAGTAGAAACAACAGCATTTTTTATATTTATTTTAATAAGA26070.30509783728115347No Hit
GGGGGGAGCAGAAGCGGAGCCTTTAAGATGAATATAAATCCTTATTTTCT25890.3029912929500983No Hit
CCGGGTTATTAGTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGT25790.30182099054395656No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT24150.2826280310832319TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTAT22940.26846737196891673TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCAGAAGCAGAGGATTTTCTAATATCCACAAAATGAAGGCAAT22250.26039228536653874No Hit
GGGGGGAGCAGAAGCGGTGCCATGGATGAACTCCACAACGAAATACTAGA22220.2600411946446962No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTG22180.2595730736822395No Hit
GGGGGGAGCAGAAGCGGTGCCTTTAAGATGAATATAAATCCTTATTTTCT20380.23850763037168804No Hit
GGGGGGAGCAGAAGCGGAGCGTTTTCAAGATGACATTGGCCAAAATTGAA19260.2254002434229005No Hit
CCGGGTTATTAGTAGAAACACGAGCATTTTTGATTCTTGGTTCTTTTTAT16450.19251474581031738No Hit
CCGGGTTATTAGTAGAAACAACAGCAACAAAAAAGAAAGGCCTGATTCTG15520.18163093343319914No Hit
GGGGGGAGCAGAAGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC14930.17472614923696284No Hit
GGGGGGAGCAGAAGCGGTGCGTTTTCAAGATGACATTGGCCAAAATTGAA14830.1735558468308211No Hit
CCGGGTTATTAGTAGTAACAAGAGCACCGATTATCACCAGAAGAGGGAGA13360.1563524014605374No Hit
GGGGGGAGCAGAAGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACAC12140.1420747121056081No Hit
GGGGGGAGCAGAAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCT11720.13715944199981275No Hit
GGGGGGAGCAGAAGCGGTGCATCTTCTCAAAACTGAAGCAAATAGGCCAA10650.12463720625409606No Hit
CCGGGTTATTAGTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGT10330.12089223855444246No Hit
CCGGGTTATTAGTAGTAACAAGAGGATTATGATGACCTCGATTATTAAAG8670.10146521861248946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCG108.551168E-4294.05
CCGGGTT78200.0292.872131
CGGGTTA78600.0291.194672
GGGGGGA58650.0286.731451
GTTATTA80100.0282.25475
GGGTTAT86950.0263.399663
TTAGTAG87450.0257.01899
TTATTAG88400.0255.420826
ATTAGTA88450.0254.944058
TATTAGT88950.0253.841497
GGAGCAG67700.0247.098975
GGGGAGC69000.0243.721733
CAGAAGC68650.0242.180639
GAGCAGA69750.0239.20436
GGTTATT98600.0232.576054
GGGAGCA75600.0221.47224
GGGGGAG76250.0220.162632
GCAGAAG92000.0181.193488
AGCAGAA106200.0157.10457
GGGGGGC200.0068061445147.01
Produced by FastQC (version 0.11.2)