FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool d3.3410000000b6f8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool d3.3410000000b6f8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences843876
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC223712.650981897814371No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA194982.3105290350715033No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG155651.8444653005891862No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG130481.5461987306191904No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA115281.3660774805777152No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG103051.2211509747877651No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC101441.202072342382056No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG98611.1685366096440708No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG80550.9545241243974233No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA77520.918618375211524No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA76120.9020282600761249No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC66940.7932445051168656No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA62050.7352976029653646No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT59650.7068574055903948No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA55570.6585090700529461No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG54910.6506880157748295No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA47740.5657229261171073No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT46640.5526878356535795TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT43190.5118050519270604No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC41840.4958074409036398No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA41020.48609037346719186No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA28580.3386753504069318No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA19110.2264550715981969No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT13730.1627016291493063No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11990.14208248605245322No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA109700.0292.257971
CGGGTTA102700.0290.851041
GGGTTAT103600.0288.891882
ATTAGTA103800.0287.343937
GGGGGAG114100.0281.63192
GGTTATT108100.0276.72993
TATTAGT109400.0272.366526
GGAGCGT1200.0269.499975
GTTATTA112800.0264.93884
TTATTAG113700.0262.71245
TTAGTAG115100.0259.13388
GGAGCGA78400.0258.375035
GAGCGAA78800.0256.50386
GCGAAAG79050.0256.250468
CGAAAGC80300.0252.078439
GAGCGTA1000.0249.96
TAGTAGA119450.0249.327779
GGGGAGC132200.0242.405443
AGCGAAA87300.0232.034367
AGCGTAA1100.0227.181827
Produced by FastQC (version 0.11.2)