FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool d2.3410000000b68e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool d2.3410000000b68e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences938181
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT894249.531636219450192TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA413164.403841049861381No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC402854.293947543171307No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG298683.1836074275646173No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA232862.4820370482881238No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA186571.9886354552053387No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC182941.9499435609972915No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG140461.4971524684469202No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT128161.366047702948578No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG108901.1607568262414183No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG108871.1604370585206907No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA104251.1111928295286304No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA90600.9656985165975435No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA88610.9444872577892752No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA69720.7431401829710897No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA66430.7080723229312894No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT63780.6798261742670124No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG57790.6159792193617223No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT52690.5616187068380195No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA34690.36975807440142144No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT30310.3230719871751826No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT25120.26775217148929686No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA22500.23982579054574754No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAACCTCGTAT20080.21403119440704937TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17080.18205442233428304No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTAT17080.18205442233428304TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT13280.14155051104211236No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT10840.11554273642292905No Hit
GGGGGGAGCAATTGTTGGCGAAATCTCACCATTGCCTTCTTTTCCAGGAC10370.11053304213152898No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA168700.0291.297941
CGGGTTA171550.0291.08581
GGGTTAT173000.0288.731022
TTAGTAG176450.0282.002628
TAGTAGA178050.0279.22089
TTATTAG179050.0279.057075
TATTAGT179250.0277.84366
GGGGAGC176950.0277.135163
ATTAGTA180000.0276.767587
GGTTATT180800.0276.437323
GGGAGCG124750.0274.202854
GTTATTA183000.0273.1144
GGAGCGA124850.0271.86395
GAGCGAA130850.0258.94856
AGCGAAA131500.0257.556737
GGGGGAG191500.0256.846282
CGAAAGC139400.0241.800649
GGGAGCA62100.0239.79164
GCGAAAG141650.0238.47878
GAGCGTA1150.0217.303766
Produced by FastQC (version 0.11.2)