FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool d1.3410000000b5ec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool d1.3410000000b5ec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences546802
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT8635715.793102439274179TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC79521.4542741248203188No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA62101.1356944561285438No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT58221.0647364128148764No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG46680.8536910984231952No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT46310.8469244808907064No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG46300.8467415993357742No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT45450.8311966671665428No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA42510.777429490016496No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT37150.6794049765728728No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT35580.650692572448528No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT29730.5437068628132303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT24790.45336337467675686TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA24660.45098591446263914No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA23820.43562386384834No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG23710.4336121667440866No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT23120.4228221550030907TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA22480.41111773548743424No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT22290.4076429859437237No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT18540.33906240284417394No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT16370.2993771054239011No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA15530.284015054809602No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA12310.2251271941214553No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA12100.22128668146788052No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA10290.1881851200251645No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT10170.18599054136597892TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG9740.1781266345038972No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAGCTCGTAT6550.11978741848054689TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG6240.11411809027765077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA43400.0291.629031
GGGTTAT43900.0288.307532
GGGGAGC40950.0270.30773
GTTATTA47350.0267.611424
GGGGGGA42050.0263.236631
GGGAGCG28200.0260.1174
GGTTATT48850.0259.394073
GGAGCGA27950.0257.710175
TTATTAG49900.0253.64135
TTAGTAG50400.0249.666698
TATTAGT52000.0242.83276
GGGAGCA15450.0242.621374
ATTAGTA52300.0241.720867
TAGTAGA55850.0225.040289
GGGGGAG49450.0223.84432
GGAGCGT600.0220.55
GAGCGAA33500.0215.014926
CGAAAGC33850.0211.92329
AGCGAAA34700.0207.155627
GCGAAAG37150.0193.098258
Produced by FastQC (version 0.11.2)