FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool c4.3410000000b757.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool c4.3410000000b757.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences808888
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT397014.908096052852805TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC208742.580579758878856No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA165252.042928069151724No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG124951.544713235948611No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC120451.4890813066827546No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG103221.2760728308492646No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA78450.9698499668680955No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG66320.8198910108692427No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT60480.7476931293331092No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG60100.7429953219728813No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA59450.734959598856702No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG53660.6633798498679668No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT45210.5589154493576366No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT38180.47200601319342106No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT33050.4085856138303449No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA29170.3606185281522287No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT26720.33033003332970695No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA25190.3114151773793158No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA20250.2503436816963535No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT18790.23229421131232011No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA16120.1992859332812454No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACA13910.17196447468623594No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT11300.13969795571203925No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTAT11120.137472678541405TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT10960.1354946543897301TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA9930.12276112391332299No Hit
GGGGGGAGCGAAAGCAGGTACTGGGCCATAAGGACCAGGAGTGGAGGAAA9910.12251387089436362No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA77150.0290.95141
GGGTTAT77850.0288.335272
GGGGGGA77350.0282.597261
GGTTATT80500.0278.478273
GGGGAGC80250.0273.117773
TTAGTAG83750.0266.4438
GGGAGCG52700.0264.99054
GGAGCGA52150.0263.275185
TATTAGT85200.0261.908456
TAGTAGA85650.0260.7049
TTATTAG87150.0257.734925
GTTATTA87050.0257.693274
ATTAGTA87100.0256.870277
CGAAAGC55100.0248.379329
GGGGGAG93200.0235.641632
GCGAAAG60300.0227.447748
GGAGCGT1050.0223.999985
AGCGAAA61250.0223.680017
GAGCGAA64050.0214.360666
GGGAGCA42200.0189.845984
Produced by FastQC (version 0.11.2)