FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool c3.3410000000b6eb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool c3.3410000000b6eb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences814706
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT315923.87771785159309TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA229082.811811868330416No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC185022.271003282165591No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA159301.9553065768510358No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA152251.8687722933180804No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG106161.3030467432423476No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG103561.271133390450052No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG103211.2668373621895506No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG86341.0597688000333862No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC85421.0484763828914971No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA84721.0398843263704944No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA84471.0368157347558506No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG81691.0026929960010114No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT55130.676685822861253No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA50420.6188735568413637No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT48800.5989890831784718No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT47980.58892410268244No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT45090.5534511836171576No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT44920.5513645413191998No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA44350.544368152437812No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT40410.49600714859102546No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA30360.3726497656823443No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA24000.2945847950058058No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14810.18178336725149932No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT9010.11059204179176291TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA8840.10850539949380514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA102250.0289.111971
GGGTTAT103350.0286.31932
GGGGGGA106950.0285.340821
GGTTATT106600.0277.590063
GGGGAGC112400.0270.589843
TTAGTAG110700.0265.848248
GGGAGCG69150.0264.23864
TATTAGT111900.0263.260046
ATTAGTA112150.0262.67327
TAGTAGA112450.0261.580269
GTTATTA113350.0260.800174
GGAGCGA69900.0259.721045
TTATTAG114550.0258.196445
GGGGGAG119600.0254.791822
CGAAAGC75950.0237.870979
AGCGAAA76850.0235.46787
GCGAAAG79200.0228.666678
GAGCGAA80850.0224.363636
GGGAGCA56450.0215.617364
GGAGCGT750.0196.05
Produced by FastQC (version 0.11.2)