FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool c2.3410000000b671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool c2.3410000000b671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1147070
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT472294.1173598821344815TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA306072.6682765655103875No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC301272.626430819392016No Hit
GGGGGGAGCGAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA220311.9206325681954894No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG201551.7570854437828556No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG183711.6015587540429093No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTTAATTGTCGTACTCTTC147141.2827464758035692No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAACATTAATTG145421.2677517501111528No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA142591.243080195628863No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA118671.0345488941389802No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG114250.9960159362549801No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT106010.9241807387517762No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG100630.8772786316441019No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT84150.7336082366376944No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA82850.7222750137306355No Hit
GGGGGGAGCAAAAGCAGGTACCGATTCGAAATGGAAGATTTTGTGCGACA73780.6432039892944633No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT68610.5981326335794677No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT61250.5339691561979653No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT59950.5226359332909064No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTTCTAAAATTGCGAAAGCTTA57680.5028463825224266No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCTTT57200.4986618079105896No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA41610.3627503116636299No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA34230.2984124770066343No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT23100.2013826531946612No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA12480.108798939907765No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA132850.0292.561551
GGGTTAT134100.0290.16332
GGGGGGA165150.0286.078121
GGTTATT139200.0279.63793
GGGGAGC173350.0271.782533
TTAGTAG142600.0271.527348
TAGTAGA143450.0269.815989
TTATTAG146250.0266.157935
TATTAGT146500.0264.499666
GTTATTA147400.0264.08144
GGGAGCG111600.0261.991944
GGAGCGA111750.0258.74635
ATTAGTA150450.0257.359927
GGGGGAG186800.0252.921862
GGGAGCA76550.0233.510124
AGCGAAA127350.0226.935237
CGAAAGC128650.0224.75639
GAGCGAA130100.0222.138376
GCGAAAG133650.0216.347928
GGAGCAT1300.0214.846135
Produced by FastQC (version 0.11.2)