##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n01 clinical flu pool c1.3410000000b5c2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	658553
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.77612128408799	34.0	34.0	34.0	34.0	34.0
2	33.85396315862201	34.0	34.0	34.0	34.0	34.0
3	33.83418494790852	34.0	34.0	34.0	34.0	34.0
4	33.83866446588202	34.0	34.0	34.0	34.0	34.0
5	33.868529943679555	34.0	34.0	34.0	34.0	34.0
6	37.62432636401322	38.0	38.0	38.0	38.0	38.0
7	37.66640650031205	38.0	38.0	38.0	38.0	38.0
8	37.603591510478275	38.0	38.0	38.0	37.0	38.0
9	37.54977503708889	38.0	38.0	38.0	37.0	38.0
10-14	37.38975298874958	38.0	38.0	38.0	37.0	38.0
15-19	37.42264328003973	38.0	38.0	38.0	37.0	38.0
20-24	37.49458919783221	38.0	38.0	38.0	37.0	38.0
25-29	37.40962504156841	38.0	38.0	38.0	37.0	38.0
30-34	37.324216881556985	38.0	38.0	38.0	37.0	38.0
35-39	37.34037564174789	38.0	38.0	38.0	37.0	38.0
40-44	37.13140536904395	38.0	38.0	38.0	36.4	38.0
45-49	37.31579447667842	38.0	38.0	38.0	37.0	38.0
50-54	37.35285846393533	38.0	38.0	38.0	37.0	38.0
55-59	37.21065214189291	38.0	38.0	38.0	37.0	38.0
60-64	37.23130044202973	38.0	38.0	38.0	37.0	38.0
65-69	37.214650908886604	38.0	38.0	38.0	37.0	38.0
70-74	37.15143868450983	38.0	38.0	38.0	36.4	38.0
75-79	36.08951990196689	38.0	38.0	38.0	33.2	38.0
80-84	35.82926142618741	38.0	38.0	38.0	33.0	38.0
85-89	35.867121097314865	38.0	38.0	38.0	33.6	38.0
90-94	35.64844894792067	38.0	37.8	38.0	31.4	38.0
95-99	35.47222258497038	38.0	37.0	38.0	29.0	38.0
100-104	35.77770748899481	38.0	38.0	38.0	32.0	38.0
105-109	35.731668369895814	38.0	38.0	38.0	31.8	38.0
110-114	35.60761244728973	38.0	37.8	38.0	30.2	38.0
115-119	35.54594239188039	38.0	37.0	38.0	29.6	38.0
120-124	35.512851964838056	38.0	37.2	38.0	29.2	38.0
125-129	35.53235168619686	38.0	37.0	38.0	29.6	38.0
130-134	35.53183388428873	38.0	37.0	38.0	29.8	38.0
135-139	35.48370320991628	38.0	37.0	38.0	29.2	38.0
140-144	35.43678428311769	38.0	37.0	38.0	28.4	38.0
145-149	35.38560723282713	38.0	37.0	38.0	27.8	38.0
150-154	35.316931515003354	38.0	37.0	38.0	27.0	38.0
155-159	35.27747622438892	38.0	37.0	38.0	27.0	38.0
160-164	35.23644232127103	38.0	37.0	38.0	27.0	38.0
165-169	35.14619081531782	38.0	37.0	38.0	26.2	38.0
170-174	35.04526864200755	38.0	37.0	38.0	25.2	38.0
175-179	34.89452663642866	38.0	37.0	38.0	24.2	38.0
180-184	34.78402953141205	38.0	37.0	38.0	23.6	38.0
185-189	34.56138685876461	38.0	36.0	38.0	23.0	38.0
190-194	34.42122380431035	38.0	36.0	38.0	22.6	38.0
195-199	34.13973453920944	38.0	35.4	38.0	21.2	38.0
200-204	34.060571282797284	38.0	35.0	38.0	21.0	38.0
205-209	33.84210291350887	38.0	34.8	38.0	19.6	38.0
210-214	33.62815900922173	38.0	34.4	38.0	18.6	38.0
215-219	32.73942203588777	38.0	31.8	38.0	15.2	38.0
220-224	32.68127272975751	37.6	32.6	38.0	15.8	38.0
225-229	32.283887553469505	37.0	30.4	38.0	14.4	38.0
230-234	31.875014767224503	37.0	29.0	38.0	12.8	38.0
235-239	31.634054662267125	37.0	28.0	38.0	11.0	38.0
240-244	30.883222458936483	37.0	25.4	38.0	10.6	38.0
245-249	29.98836722329106	36.0	23.4	37.6	10.0	38.0
250-254	29.187020634633814	35.0	21.6	37.0	9.6	37.8
255-259	28.693042777118926	34.2	20.6	37.0	9.0	37.6
260-264	27.665565565717564	32.6	18.6	37.0	9.0	37.2
265-269	26.67924950611416	31.2	16.6	37.0	8.8	37.0
270-274	26.035780871091617	29.8	15.8	37.0	8.4	37.0
275-279	25.272832710503177	28.4	14.6	36.8	8.0	37.0
280-284	24.01567072050389	25.8	13.2	35.4	8.0	37.0
285-289	22.954769016313037	23.8	12.2	33.6	8.0	37.0
290-294	22.195293317318423	22.2	11.4	32.4	8.0	37.0
295-299	20.90839734994754	20.0	9.6	30.6	7.8	37.0
300	14.949314633750054	13.0	8.0	19.0	7.0	28.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.30070351364707193
1101	2	0.002935767460975569
1101	3	-0.001808530809505271
1101	4	0.028211259156023516
1101	5	0.02597987204489982
1101	6	0.09766470634377811
1101	7	-0.048644474661820425
1101	8	-0.057820198448496285
1101	9	-0.03607422139742056
1101	10-14	0.1885700357773672
1101	15-19	0.12600734849181805
1101	20-24	0.004906355116780503
1101	25-29	-0.04675882066045034
1101	30-34	0.07544806166437468
1101	35-39	-0.04420992183055006
1101	40-44	-0.08417458062987038
1101	45-49	-0.0717545325578044
1101	50-54	-0.007474245961468284
1101	55-59	-0.05313726698503274
1101	60-64	-0.05467700253493035
1101	65-69	-0.07911199070842656
1101	70-74	-0.029760922641635545
1101	75-79	0.08748248119274393
1101	80-84	0.04878251390773869
1101	85-89	0.037882403608726634
1101	90-94	0.18044007917961125
1101	95-99	0.3636755905607387
1101	100-104	0.02022304660987828
1101	105-109	-0.06754891101538618
1101	110-114	-0.07806189690469978
1101	115-119	0.09553166860838758
1101	120-124	0.0405432315566614
1101	125-129	-0.013006559637339876
1101	130-134	-0.024290472402498153
1101	135-139	0.034496483470633166
1101	140-144	0.08769749720750042
1101	145-149	-0.05186554083282857
1101	150-154	0.013270495924160741
1101	155-159	-0.06826937634991737
1101	160-164	-0.11999028281637436
1101	165-169	-0.17767225917419438
1101	170-174	-0.1978467339544352
1101	175-179	-0.13844532411170718
1101	180-184	-0.14060253005109047
1101	185-189	-0.023426687822670544
1101	190-194	-0.11633699433433264
1101	195-199	0.0019219127574459094
1101	200-204	-0.14900232511113387
1101	205-209	-0.07202895308786594
1101	210-214	-0.04122831756453138
1101	215-219	0.6523518713959504
1101	220-224	0.1693887757887964
1101	225-229	-0.25843998039453453
1101	230-234	0.016888055493133436
1101	235-239	-0.7469724545715231
1101	240-244	-0.43604357819452133
1101	245-249	-0.0822030188395999
1101	250-254	-0.2399180001404666
1101	255-259	-0.4890320869015383
1101	260-264	-1.4859037824525139
1101	265-269	-1.297503626359589
1101	270-274	-1.4927932554899002
1101	275-279	-1.5999658013611011
1101	280-284	-1.1420594884523751
1101	285-289	-0.9701844057506754
1101	290-294	-0.938477334744011
1101	295-299	-0.30225069916022207
1101	300	-0.6963676562359247
1102	1	-0.007555816209915633
1102	2	-0.00927710941881088
1102	3	0.011529662870053414
1102	4	0.04226122121075093
1102	5	-0.004819429699324473
1102	6	0.04994385781531463
1102	7	0.017367721245747703
1102	8	0.019444851210309366
1102	9	0.06027847633042427
1102	10-14	0.20574505380727004
1102	15-19	0.11900373622803073
1102	20-24	0.01749978453621992
1102	25-29	0.04462545098681403
1102	30-34	0.1330053920144607
1102	35-39	0.006407588299964573
1102	40-44	-0.027755011241872296
1102	45-49	-0.0037455513921571537
1102	50-54	0.055362551677909266
1102	55-59	0.06523569021616282
1102	60-64	0.0240781412878448
1102	65-69	0.05057101311123802
1102	70-74	0.010298281403343879
1102	75-79	-0.07507353717810616
1102	80-84	-0.14843508452606358
1102	85-89	-0.1878805948058968
1102	90-94	0.07235379336925263
1102	95-99	0.2461553098757534
1102	100-104	-0.12794198937920243
1102	105-109	-0.1814052029378459
1102	110-114	-0.054137869144156525
1102	115-119	0.059897558405268114
1102	120-124	-0.005251977126597751
1102	125-129	0.003879037344631797
1102	130-134	-0.01272073245721117
1102	135-139	-0.07682541024176714
1102	140-144	-0.05229882610817782
1102	145-149	-0.10853525045332901
1102	150-154	0.03327938910077677
1102	155-159	0.03538452509922507
1102	160-164	0.010769329591958865
1102	165-169	0.03265683549729914
1102	170-174	-0.04091811662342337
1102	175-179	-0.09641064072293659
1102	180-184	-5.344328294967227E-4
1102	185-189	0.1215548423379218
1102	190-194	0.1558842729276364
1102	195-199	0.3235135085484231
1102	200-204	0.1813117485765332
1102	205-209	0.07617108996389987
1102	210-214	0.20846222817168325
1102	215-219	1.0549774010977373
1102	220-224	0.7998509538628724
1102	225-229	0.5697351114832969
1102	230-234	0.6636428473598031
1102	235-239	0.6794029474676151
1102	240-244	0.916407717522457
1102	245-249	0.8752686793328976
1102	250-254	1.0346087681640022
1102	255-259	1.0284697160878942
1102	260-264	0.9792488863646476
1102	265-269	0.8540308237011409
1102	270-274	-0.07568674320208046
1102	275-279	-0.03528459660278216
1102	280-284	0.24639195181800844
1102	285-289	0.18643876395973535
1102	290-294	0.6769157798004173
1102	295-299	0.6533328173526485
1102	300	0.04313548858615768
1103	1	-0.0850602373877436
1103	2	-0.05130568666030655
1103	3	-0.046905005424235924
1103	4	-0.006877235993812292
1103	5	0.0015361112493863516
1103	6	0.04623945854774547
1103	7	-0.012095320837609336
1103	8	0.08977522665829696
1103	9	0.019002673084571597
1103	10-14	0.23188462217510875
1103	15-19	0.1484893145807007
1103	20-24	0.0228471404371291
1103	25-29	0.07995950422874643
1103	30-34	0.08502348363065693
1103	35-39	0.015334767979766184
1103	40-44	0.06959541209567988
1103	45-49	0.06492684324295084
1103	50-54	0.08540903273335942
1103	55-59	0.11329052847148091
1103	60-64	-0.003833967261847704
1103	65-69	0.07607424487486014
1103	70-74	0.11250902682174768
1103	75-79	0.1778766795769826
1103	80-84	0.2521367733076403
1103	85-89	0.16610253429249866
1103	90-94	0.43165516902069356
1103	95-99	0.4822703074499728
1103	100-104	0.10974866902417091
1103	105-109	-0.04569491919995272
1103	110-114	0.18474251647644735
1103	115-119	0.2840326508196185
1103	120-124	0.3158294507964712
1103	125-129	0.2078294575521511
1103	130-134	0.12424845232454373
1103	135-139	0.18003923129070643
1103	140-144	0.27207665317879304
1103	145-149	0.22004699910455372
1103	150-154	0.2216995027211368
1103	155-159	0.21501643270052995
1103	160-164	0.1754919300270359
1103	165-169	0.16591003987308284
1103	170-174	0.2068478191795151
1103	175-179	0.11960294363939994
1103	180-184	0.20591563524882162
1103	185-189	0.3184651824164817
1103	190-194	0.3562392191692325
1103	195-199	0.4953750982944243
1103	200-204	0.33448325897608555
1103	205-209	0.4766246323837393
1103	210-214	0.6393829286740598
1103	215-219	1.1818941577529856
1103	220-224	0.8221368201789758
1103	225-229	0.9256707579142684
1103	230-234	1.0609363040166144
1103	235-239	0.8435555680914391
1103	240-244	1.0914869857197118
1103	245-249	1.432885155849796
1103	250-254	1.150432359255916
1103	255-259	1.106381355131976
1103	260-264	1.4851508848935246
1103	265-269	1.2789859859153303
1103	270-274	1.0112253183970594
1103	275-279	0.2577601472819069
1103	280-284	0.3976726066218319
1103	285-289	0.554345238442103
1103	290-294	0.8382820411272434
1103	295-299	0.9404073278930483
1103	300	-0.03527507842011879
2116	1	-0.03778346524901366
2116	2	-0.07339080053492353
2116	3	-0.011815938347844224
2116	4	0.037987602917219476
2116	5	0.04026308424918312
2116	6	-0.03232682560263811
2116	7	-0.07937017239247979
2116	8	-0.1009211450387042
2116	9	0.030779053104097898
2116	10-14	0.23495883673633244
2116	15-19	0.12289817487422283
2116	20-24	0.07743653561325914
2116	25-29	0.1051297159731277
2116	30-34	0.13065623598737375
2116	35-39	0.032405877597085464
2116	40-44	-0.04047196838798328
2116	45-49	0.07709630275864754
2116	50-54	0.09491400224408864
2116	55-59	0.05264617258729487
2116	60-64	0.11011331271126323
2116	65-69	0.06929426376059666
2116	70-74	0.062123789709680466
2116	75-79	0.026551316666662217
2116	80-84	0.03828264165161954
2116	85-89	0.012847944897323771
2116	90-94	0.24500514741412616
2116	95-99	0.4017351047483473
2116	100-104	0.04244244793295593
2116	105-109	-0.020348333838001054
2116	110-114	0.06095288442830338
2116	115-119	0.2095011932565214
2116	120-124	0.30871912925871925
2116	125-129	0.16874653634410208
2116	130-134	0.029082881991165266
2116	135-139	0.12898627119082562
2116	140-144	0.17957141158535705
2116	145-149	0.16203736417553216
2116	150-154	0.3472922429141505
2116	155-159	0.2997785203751775
2116	160-164	0.1831102459950884
2116	165-169	0.24130640036667472
2116	170-174	0.3120212318969777
2116	175-179	0.515095013228553
2116	180-184	0.42581384430436486
2116	185-189	0.23634645832436263
2116	190-194	0.3005403667180886
2116	195-199	0.4883342378728628
2116	200-204	0.329875778301961
2116	205-209	0.4628665299205821
2116	210-214	0.3786473450566419
2116	215-219	1.100557431388168
2116	220-224	0.5420094214497624
2116	225-229	0.5413942578582578
2116	230-234	0.6386226406629518
2116	235-239	0.3704431019017136
2116	240-244	0.44954591563440616
2116	245-249	1.0252524355028072
2116	250-254	1.1753503343203349
2116	255-259	0.67506047358399
2116	260-264	0.6329900680051956
2116	265-269	0.9895520780073248
2116	270-274	0.5413650408726944
2116	275-279	-0.3105809484111788
2116	280-284	0.9473497884851163
2116	285-289	0.4766172891455156
2116	290-294	0.6565957685003667
2116	295-299	0.9421531386195134
2116	300	0.3537551867805018
1117	1	0.004205299335417578
1117	2	-0.0021531442874263007
1117	3	-0.03165076813609602
1117	4	-0.010267066502287037
1117	5	-0.1007237757562578
1117	6	-0.10856280133926077
1117	7	-0.08893147902969645
1117	8	-0.08367110102532394
1117	9	-0.10303122522051211
1117	10-14	0.16589592161012945
1117	15-19	0.03605993604962521
1117	20-24	-0.050599187246490374
1117	25-29	-0.024108292381420426
1117	30-34	-0.004807024843913155
1117	35-39	-0.012122859138877118
1117	40-44	0.029934395146533177
1117	45-49	-0.013943213254222542
1117	50-54	-0.046794357097155626
1117	55-59	-0.02411060147203159
1117	60-64	-0.21298650963473165
1117	65-69	-0.11364326924944379
1117	70-74	7.964829068640711E-5
1117	75-79	-0.004609196129230497
1117	80-84	0.0780124795223287
1117	85-89	0.04384264728684428
1117	90-94	0.2452144910545826
1117	95-99	0.3740217198793445
1117	100-104	-0.05861291289674142
1117	105-109	-0.05767232032989256
1117	110-114	-0.12441002589643801
1117	115-119	0.11793095590436309
1117	120-124	0.06882380107894903
1117	125-129	0.008168440603000704
1117	130-134	0.05261003424546118
1117	135-139	0.2668188923076471
1117	140-144	0.22122919555167897
1117	145-149	0.21247637763563176
1117	150-154	0.15593679085672107
1117	155-159	0.0993102180112615
1117	160-164	0.05786481138297006
1117	165-169	0.12873489863014242
1117	170-174	0.24221505081793282
1117	175-179	0.11158034476933665
1117	180-184	0.03631111547480259
1117	185-189	-0.6540771904648679
1117	190-194	-0.27212236275167356
1117	195-199	-0.21264750057562765
1117	200-204	0.10182789174444906
1117	205-209	0.3455511860560563
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2108	185-189	-0.24592842006082805
2108	190-194	-0.11326120085349345
2108	195-199	-0.10246647901445982
2108	200-204	0.0956178144932025
2108	205-209	0.026062790213444487
2108	210-214	0.07029758993807889
2108	215-219	0.704604045582208
2108	220-224	0.24086950453601474
2108	225-229	0.299590146492946
2108	230-234	0.45112885561352556
2108	235-239	0.3920812059494807
2108	240-244	0.8363931806432063
2108	245-249	0.9786640097642376
2108	250-254	0.570480455976444
2108	255-259	0.3255679934677005
2108	260-264	0.39564519399731424
2108	265-269	0.5461817165078138
2108	270-274	0.6542939550526832
2108	275-279	1.1916713001207704
2108	280-284	0.5977790123802578
2108	285-289	0.786363818271127
2108	290-294	0.00875493730750776
2108	295-299	0.8655938673998982
2108	300	-0.3011511140644014
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	13.0
14	18.0
15	37.0
16	90.0
17	479.0
18	7454.0
19	14340.0
20	13367.0
21	8536.0
22	4803.0
23	3422.0
24	3487.0
25	4189.0
26	5532.0
27	7634.0
28	10388.0
29	14146.0
30	19269.0
31	26414.0
32	35890.0
33	52095.0
34	67375.0
35	106371.0
36	171344.0
37	81856.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.93863212224377	26.41883037507991	12.31411898510826	23.32841851756806
2	37.849345458907635	28.952870915476808	20.41992064420024	12.777862981415314
3	37.474736277869816	20.135053670699246	27.85910928960919	14.531100761821753
4	37.97947925223938	19.451129977389822	19.830750144635285	22.738640625735513
5	34.31250028471513	20.415213354126397	30.066524638108096	15.205761723050385
6	33.91040660356873	20.506777738466	30.29384119425468	15.28897446371059
7	16.158456494769595	48.93121738113713	19.954961863357997	14.95536426073528
8	26.167369976296516	27.35208859423615	30.57946740809016	15.90107402137717
9	22.61928804515354	20.372392199261107	30.774895870188125	26.23342388539723
10-14	24.691558614113063	34.935198837451196	22.207324239658767	18.16591830877697
15-19	22.272163364224294	32.64976395218	22.502000598281384	22.576072085314316
20-24	23.4339529240623	32.072832406807045	26.315330732682106	18.177883936448545
25-29	19.55754510267207	26.001217821496525	35.169743361582135	19.27149371424927
30-34	18.483098550913898	28.54285076523833	29.884975089324627	23.089075594523145
35-39	22.39427957962381	29.39198515533298	27.19598878146482	21.01774648357839
40-44	20.025237148718478	29.406987744342523	29.2548967205373	21.3128783864017
45-49	20.740107478061752	29.389525216649233	28.29567248194147	21.57469482334755
50-54	22.865615979275773	27.315796906247485	27.97697375913556	21.84161335534118
55-59	20.23685261474779	24.633127477970643	32.15725993200243	22.972759975279132
60-64	20.90923585497295	25.257253402535557	31.90254998458742	21.93096075790407
65-69	20.745391790789807	29.420927396883773	28.829752502835763	21.003928309490654
70-74	19.641760040573804	30.742248535805018	26.818160421408756	22.797831002212426
75-79	19.315985197850438	28.81156110442136	30.099566777465142	21.772886920263062
80-84	22.44806416491915	27.78883400424871	26.543497638003323	23.219604192828825
85-89	20.110970567289193	30.3148265971	26.115620155097613	23.458582680513185
90-94	19.510198875413217	30.20185163532776	28.818546115498677	21.46940337376035
95-99	21.02934767588941	29.848895988629614	26.517774575470764	22.603981760010207
100-104	21.1057272535392	29.767019510958114	27.089938091543125	22.03731514395956
105-109	22.455109915223222	29.08318692648883	24.491392492327876	23.970310665960067
110-114	20.958100562900782	29.12892356423857	27.88328350185938	22.02969237100127
115-119	20.89641987812674	30.972541314062802	25.645771866501256	22.485266941309202
120-124	19.34091865043512	29.813819085176135	28.006614501794086	22.838647762594658
125-129	20.104835905386505	27.444533697363767	29.773791934741773	22.676838462507952
130-134	23.156897973860257	28.849471143391998	26.74485629701977	21.24877458572798
135-139	22.476763449562906	29.62294606508512	25.54333516057174	22.356955324780238
140-144	20.65721098056556	30.204633616206205	26.750239844167346	22.387915559060886
145-149	19.841318770091398	29.286268531158466	28.631530036306874	22.240882662443266
150-154	21.673942719872205	27.7010050823548	26.575476840892076	24.04957535688092
155-159	20.463136604039462	28.30721293502573	29.318885495928193	21.910764965006614
160-164	22.533402778515928	29.169892172687696	26.99828259836338	21.298422450432994
165-169	19.005729227564068	27.338027463241378	28.883871153878278	24.772372155316276
170-174	19.689349224739694	29.819801899011928	26.676364696539228	23.81448417970915
175-179	20.035502078040796	29.855091389759064	27.477788424014467	22.631618108185673
180-184	19.575675761859713	29.522453014411898	26.647088389241258	24.254782834487127
185-189	21.73453009856458	28.222512083309926	27.279748175165857	22.76320964295964
190-194	19.608869749283656	30.451489857308374	27.225386567216308	22.714253826191666
195-199	19.89128282500659	30.64136998582346	26.455373054370128	23.01197413479982
200-204	21.706468575801797	28.685102034308553	27.39257128887121	22.215858101018444
205-209	20.37381957108995	29.29319280300902	28.30028866317517	22.032698962725856
210-214	21.256390905515577	28.51071971428268	27.968105832028705	22.26478354817304
215-219	21.637384276553075	27.947098546995775	27.810131549057264	22.605385627393883
220-224	18.764715975783268	28.402968326011724	27.826188628705662	25.00612706949934
225-229	20.51642359818292	29.614833990431134	26.804913322007483	23.06382908937846
230-234	19.85185702593413	28.656949402705628	27.882706479205165	23.608487092155073
235-239	20.343480327323693	28.537870148644075	25.96298247825156	25.15566704578067
240-244	20.86996794487308	30.860902615279258	25.21910916813074	23.05002027171693
245-249	20.370904088205506	29.428914605202618	25.777788575862537	24.422392730729342
250-254	19.77341231457453	30.329920294949687	25.57160927062818	24.325058119847608
255-259	19.50652415219428	29.52233153595838	27.2880390796185	23.68310523222884
260-264	19.561645000478322	30.7436151684071	26.389614807008698	23.30512502410588
265-269	19.535320478479477	30.04958751978581	26.407753486128975	24.007338515605735
270-274	18.578428767312577	31.368834399053686	25.776968596301288	24.275768237332453
275-279	18.35701506607065	31.05188560488562	24.655980403083973	25.93511892595975
280-284	16.88978715456463	31.444758432502773	27.24497496784617	24.420479445086425
285-289	19.633438766507783	29.497853627574393	26.920688236178407	23.948019369739413
290-294	18.40690119094439	31.416241365539292	26.723467966891047	23.453389476625265
295-299	18.081976697395653	32.104629392015525	24.487171116068108	25.326222794520714
300	17.293976339034216	34.22093590037552	24.575698539069748	23.909389221520517
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.25
10	0.25
11	0.0
12	0.0
13	0.25
14	0.0
15	0.0
16	0.0
17	0.0
18	0.25
19	0.0
20	0.25
21	0.25
22	1.5
23	2.0
24	3.5
25	5.75
26	7.5
27	9.75
28	16.75
29	29.0
30	57.5
31	104.0
32	170.0
33	280.5
34	479.75
35	724.5
36	1089.25
37	1988.0
38	4590.0
39	9179.75
40	15044.5
41	20086.25
42	29456.25
43	31575.5
44	30325.25
45	20600.25
46	14214.0
47	10862.25
48	9236.75
49	7764.25
50	6840.5
51	2476.5
52	572.0
53	228.25
54	174.0
55	133.0
56	130.0
57	86.5
58	38.75
59	31.75
60	17.0
61	15.0
62	15.0
63	13.25
64	8.75
65	10.25
66	9.0
67	8.75
68	6.25
69	7.25
70	3.25
71	3.25
72	2.25
73	1.75
74	1.5
75	3.0
76	2.0
77	3.75
78	1.5
79	3.25
80	1.75
81	1.5
82	1.5
83	0.5
84	0.0
85	0.0
86	0.25
87	0.75
88	0.25
89	0.0
90	0.0
91	0.0
92	0.25
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	3.0369613379636874E-5
135-139	0.0
140-144	6.073922675927375E-5
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	3.0369613379636874E-5
200-204	0.0
205-209	0.0
210-214	0.0
215-219	3.0369613379636874E-5
220-224	0.0
225-229	9.110884013891061E-5
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	3.0369613379636874E-5
270-274	0.0
275-279	6.073922675927375E-5
280-284	0.0
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	658553.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.712372300012838
#Duplication Level	Percentage of deduplicated	Percentage of total
1	57.01093885155443	5.537114632995629
2	3.3869594273989083	0.65790821847873
3	2.0511152545587263	0.5976358494752987
4	1.5739879019042848	0.61148625996042
5	1.6808495776599794	0.816251843927653
6	1.7592917535893975	1.0252137897121605
7	1.5122356037823341	1.0281176632488114
8	1.6104249544201144	1.2512837374847485
9	1.7101026563866104	1.494823830306092
>10	26.532753554065536	52.68777865281784
>50	1.0402450143064499	6.415630408212951
>100	0.08202713692347778	1.3167769524445356
>500	0.006331433407814554	0.39125025684853604
>1k	0.023742711134577748	6.146514155353373
>5k	0.01424562668049688	8.993016483852218
>10k+	0.004748542226832293	11.029197264881015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT	39631	6.0178907392419445	TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	17741	2.6939365548406884	No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	14371	2.1822085693938074	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	9075	1.3780212071010232	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	7503	1.1393160459370772	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	7147	1.0852581341213237	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	6985	1.0606587472838178	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	6431	0.9765349182222235	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	5488	0.8333421911372356	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	5379	0.8167907518453336	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	5336	0.8102612849687117	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	5154	0.7826249367932421	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	4451	0.6758757457638186	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4304	0.6535540799297854	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4287	0.6509726627925163	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	3676	0.5581934939177257	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3540	0.5375421568195726	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	3005	0.456303441029044	No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	2703	0.4104453248257923	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	2689	0.40831945188921775	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	2061	0.31295886587715793	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2050	0.3112885371412779	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	1976	0.30005178019081225	No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	1640	0.24903082971302232	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1395	0.21182805332296714	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT	1164	0.1767511498694866	TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT	1041	0.1580738376410099	TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	839	0.12740052812757668	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.003796201672454609	0.0	0.0
2	0.006529466876621927	0.0	0.0
3	0.012451541485651117	0.0	0.0
4	0.020347640964356702	0.0	0.0
5	0.025358627171996787	0.0	0.0
6	0.031280701781025974	0.0	0.0
7	0.04327669906598254	0.0	0.0
8	0.05527269635093911	0.0	0.0
9	0.07000195884006298	0.0	0.0
10-14	0.11273200486521207	0.0	0.0
15-19	0.17192238134212431	0.0	0.0
20-24	0.23332973959575004	0.0	0.0
25-29	0.2713828651604351	0.0	0.0
30-34	0.3095574691786386	0.0	0.0
35-39	0.3365864250865155	0.0	0.0
40-44	0.3637368594479108	0.0	0.0
45-49	0.3912213595564822	0.0	0.0
50-54	0.4177340320369051	0.0	0.0
55-59	0.44527927137223583	0.0	0.0
60-64	0.47443410021668714	0.0	0.0
65-69	0.5096628517370659	0.0	0.0
70-74	0.5541543353382339	0.0	0.0
75-79	0.6061471134441723	0.0	0.0
80-84	0.6618146147690467	0.0	0.0
85-89	0.7244974967846172	0.0	0.0
90-94	0.8066776705899145	0.0	0.0
95-99	0.9004286670928536	0.0	0.0
100-104	1.0049608763455637	0.0	0.0
105-109	1.119758014920591	0.0	0.0
110-114	1.2565427535824756	0.0	0.0
115-119	1.4138877205023743	0.0	0.0
120-124	1.5975024029956586	0.0	0.0
125-129	1.802041749107513	0.0	0.0
130-134	2.033306354993448	0.0	0.0
135-139	2.2973397737160104	0.0	0.0
140-144	2.6062594810136774	0.0	0.0
145-149	2.9701178189151065	0.0	0.0
150-154	3.384389715026733	0.0	0.0
155-159	3.8631666699567075	0.0	0.0
160-164	4.405507225690263	0.0	0.0
165-169	5.01794084910402	0.0	0.0
170-174	5.7213618342031705	0.0	0.0
175-179	6.507266689241414	0.0	1.822176802778212E-4
180-184	7.827038977880292	0.0	3.036961337963687E-4
185-189	9.980062348816267	0.0	3.036961337963687E-4
190-194	10.980194456634468	0.0	3.036961337963687E-4
195-199	12.073530907914776	0.0	3.036961337963687E-4
200-204	13.23896482135834	0.0	3.036961337963687E-4
205-209	14.43753198299909	0.0	3.036961337963687E-4
210-214	15.666893932606792	0.0	3.036961337963687E-4
215-219	16.964951947679232	0.0	3.036961337963687E-4
220-224	18.32092481546664	0.0	3.036961337963687E-4
225-229	19.736908039292206	0.0	3.036961337963687E-4
230-234	21.171386357665973	0.0	3.036961337963687E-4
235-239	22.603799542329927	0.0	3.036961337963687E-4
240-244	23.956431752645575	0.0	3.036961337963687E-4
245-249	25.288777061223623	0.0	3.036961337963687E-4
250-254	26.664216851187376	0.0	3.036961337963687E-4
255-259	28.024562943301454	0.0	3.036961337963687E-4
260-264	29.386883060285204	0.0	3.036961337963687E-4
265-269	30.729159232438395	0.0	3.036961337963687E-4
270-274	32.07635528195908	0.0	3.036961337963687E-4
275-279	33.35558413673614	0.0	3.036961337963687E-4
280-284	34.59463399301195	0.0	3.036961337963687E-4
285-288	35.72776223022293	0.0	3.036961337963687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	6830	0.0	290.98685	1
GGGTTAT	6890	0.0	288.45285	2
GGGGAGC	7515	0.0	270.33133	3
TTATTAG	7385	0.0	269.31754	5
GGGAGCG	4850	0.0	263.99384	4
GGTTATT	7550	0.0	263.2371	3
GTTATTA	7590	0.0	261.8498	4
GGGGGGA	7755	0.0	261.3965	1
GGAGCGA	4870	0.0	259.28748	5
GGGAGCA	2935	0.0	255.93526	4
TTAGTAG	7775	0.0	254.10677	8
GGAGCGT	65	0.0	248.76923	5
TATTAGT	8140	0.0	242.89311	6
ATTAGTA	8180	0.0	242.0648	7
GGGGGAG	8580	0.0	236.60489	2
TAGTAGA	8495	0.0	232.56975	9
CGAAAGC	5640	0.0	223.8883	9
GAGCGAA	5665	0.0	223.15974	6
AGCGAAA	5660	0.0	222.83746	7
GCGAAAG	5925	0.0	213.11897	8
>>END_MODULE