FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool c1.3410000000b5c2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool c1.3410000000b5c2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658553
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT396316.0178907392419445TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA177412.6939365548406884No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC143712.1822085693938074No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG90751.3780212071010232No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT75031.1393160459370772No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT71471.0852581341213237No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT69851.0606587472838178No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT64310.9765349182222235No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA54880.8333421911372356No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG53790.8167907518453336No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG53360.8102612849687117No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT51540.7826249367932421No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA44510.6758757457638186No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT43040.6535540799297854No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT42870.6509726627925163No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA36760.5581934939177257No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA35400.5375421568195726No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG30050.456303441029044No Hit
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA27030.4104453248257923No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT26890.40831945188921775No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20610.31295886587715793No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA20500.3112885371412779No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA19760.30005178019081225No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG16400.24903082971302232No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13950.21182805332296714No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT11640.1767511498694866TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT10410.1580738376410099TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG8390.12740052812757668No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA68300.0290.986851
GGGTTAT68900.0288.452852
GGGGAGC75150.0270.331333
TTATTAG73850.0269.317545
GGGAGCG48500.0263.993844
GGTTATT75500.0263.23713
GTTATTA75900.0261.84984
GGGGGGA77550.0261.39651
GGAGCGA48700.0259.287485
GGGAGCA29350.0255.935264
TTAGTAG77750.0254.106778
GGAGCGT650.0248.769235
TATTAGT81400.0242.893116
ATTAGTA81800.0242.06487
GGGGGAG85800.0236.604892
TAGTAGA84950.0232.569759
CGAAAGC56400.0223.88839
GAGCGAA56650.0223.159746
AGCGAAA56600.0222.837467
GCGAAAG59250.0213.118978
Produced by FastQC (version 0.11.2)