FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool b4.3410000000b74a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool b4.3410000000b74a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences596992
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTAT260784.368232740137222TruSeq Adapter, Index 1 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC244864.1015625No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA198173.3194749678387647No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG133332.2333632611492282No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG129712.1727259326758146No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC86141.4429004073756433No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG81661.367857525728988No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG73881.2375375214408233No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA63531.0641683640651802No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG58310.976730006432247No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA47210.7907978666380788No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC46620.780914987135506No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA39430.6604778623499142No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG35430.5934752894511149No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA32660.5470760077186965No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC30380.5088845411663808No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA22730.3807421204974271No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA22210.3720317860205832No Hit
GGGGGGAGCGAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG21620.3621489065180103No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT17710.29665389150943394No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA15230.2551122963121784No Hit
GGGGGGAGCAAAAGCAGGCCTACCAGAAGCGAATGGGAGTGCAGATGCAG15210.25477728344768436No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT13920.2331689536878216No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10580.1772218053173242No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT8290.1388628323327616No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8090.13551270368782162No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA7960.13333512006861065No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGACCTCGTAT7490.12546231775300173TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGAACTCGTAT7250.12144216337907376TruSeq Adapter, Index 1 (97% over 36bp)
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA6620.11088925814751287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGCG108.5505226E-4294.04
GGGGCGC157.3374085E-6294.03
GGGGGGA86050.0292.9751
CGGGTTA77750.0292.86561
GGGTTAT78300.0290.808442
TTATTAG79800.0285.34215
TATTAGT80350.0282.108256
ATTAGTA80650.0281.05897
GGTTATT81750.0278.535773
GGAGCGA59750.0264.969065
GGGGGAG95350.0264.862062
CGAAAGC59800.0263.518379
GTTATTA86500.0263.240454
GGAGCGT950.0263.05265
GAGCGAA60150.0262.71826
GCGAAAG60250.0261.55028
AGCGAAA60550.0260.49717
TAGTAGA87400.0258.343269
TTAGTAG89550.0252.469018
GGGGAGC105050.0240.26563
Produced by FastQC (version 0.11.2)