##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE77A l01n01 clinical flu pool b3.3410000000b6de.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693583
Sequences flagged as poor quality	0
Sequence length	300
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.78386869343684	34.0	34.0	34.0	34.0	34.0
2	33.849976138400166	34.0	34.0	34.0	34.0	34.0
3	33.83370267148993	34.0	34.0	34.0	34.0	34.0
4	33.82241346745811	34.0	34.0	34.0	34.0	34.0
5	33.85340038611096	34.0	34.0	34.0	34.0	34.0
6	37.59243810762374	38.0	38.0	38.0	37.0	38.0
7	37.63653088383077	38.0	38.0	38.0	37.0	38.0
8	37.56505998561095	38.0	38.0	38.0	37.0	38.0
9	37.518775690869006	38.0	38.0	38.0	37.0	38.0
10-14	37.35931128646463	38.0	38.0	38.0	37.0	38.0
15-19	37.416227329677916	38.0	38.0	38.0	37.0	38.0
20-24	37.469756900039364	38.0	38.0	38.0	37.0	38.0
25-29	37.38950464472168	38.0	38.0	38.0	37.0	38.0
30-34	37.28153083336817	38.0	38.0	38.0	37.0	38.0
35-39	37.31233666338419	38.0	38.0	38.0	37.0	38.0
40-44	37.127603473556874	38.0	38.0	38.0	36.6	38.0
45-49	37.28802407210096	38.0	38.0	38.0	37.0	38.0
50-54	37.31091938527905	38.0	38.0	38.0	37.0	38.0
55-59	37.16296996898713	38.0	38.0	38.0	37.0	38.0
60-64	37.191243153306814	38.0	38.0	38.0	37.0	38.0
65-69	37.17861366267628	38.0	38.0	38.0	37.0	38.0
70-74	37.112554661806875	38.0	38.0	38.0	36.4	38.0
75-79	36.16422201812905	38.0	38.0	38.0	34.4	38.0
80-84	35.9478285944148	38.0	38.0	38.0	33.8	38.0
85-89	35.988384951764964	38.0	38.0	38.0	34.0	38.0
90-94	35.765849508998926	38.0	37.8	38.0	32.2	38.0
95-99	35.5920632426112	38.0	37.0	38.0	30.6	38.0
100-104	35.90123258499704	38.0	38.0	38.0	33.4	38.0
105-109	35.85338279629114	38.0	38.0	38.0	32.8	38.0
110-114	35.736298323344144	38.0	37.8	38.0	31.2	38.0
115-119	35.67204790198145	38.0	37.0	38.0	31.0	38.0
120-124	35.63279376801334	38.0	37.2	38.0	30.6	38.0
125-129	35.647680522734845	38.0	37.0	38.0	30.8	38.0
130-134	35.66196460985924	38.0	37.0	38.0	30.8	38.0
135-139	35.60425269938854	38.0	37.0	38.0	30.6	38.0
140-144	35.548921470105235	38.0	37.0	38.0	30.0	38.0
145-149	35.50907995149824	38.0	37.0	38.0	29.8	38.0
150-154	35.44891354026843	38.0	37.0	38.0	29.0	38.0
155-159	35.41363066857175	38.0	37.0	38.0	28.6	38.0
160-164	35.37488058386667	38.0	37.0	38.0	28.0	38.0
165-169	35.278845358089804	38.0	37.0	38.0	27.2	38.0
170-174	35.19798351459018	38.0	37.0	38.0	26.8	38.0
175-179	35.06039565560287	38.0	37.0	38.0	25.4	38.0
180-184	34.96930864799166	38.0	37.0	38.0	25.0	38.0
185-189	34.7428016545965	38.0	36.0	38.0	24.0	38.0
190-194	34.610855225690365	38.0	36.0	38.0	23.6	38.0
195-199	34.32733299403244	38.0	35.4	38.0	22.6	38.0
200-204	34.278140611866206	38.0	35.4	38.0	22.6	38.0
205-209	34.039960033622506	38.0	34.8	38.0	21.4	38.0
210-214	33.850157227036995	38.0	34.4	38.0	20.6	38.0
215-219	32.96722468687958	38.0	32.6	38.0	16.6	38.0
220-224	32.925809888650676	37.6	33.2	38.0	17.2	38.0
225-229	32.5646542663243	37.0	31.4	38.0	16.2	38.0
230-234	32.14303147568496	37.0	29.8	38.0	14.6	38.0
235-239	31.905898789330188	37.0	29.0	38.0	14.6	38.0
240-244	31.14921357645732	37.0	26.4	38.0	11.6	38.0
245-249	30.32542867976868	36.0	24.0	37.6	10.2	38.0
250-254	29.53972891492439	35.0	22.8	37.0	9.8	37.8
255-259	29.07616045952683	34.4	21.6	37.0	9.8	37.6
260-264	28.14730839712046	32.6	20.0	37.0	9.0	37.2
265-269	27.169528376560557	31.6	18.4	37.0	9.0	37.0
270-274	26.534551164027953	30.2	17.6	37.0	8.8	37.0
275-279	25.783812752042657	29.0	16.6	36.8	8.2	37.0
280-284	24.539922114584698	26.6	14.6	35.4	8.0	37.0
285-289	23.502481462204237	25.0	13.0	33.8	8.0	37.0
290-294	22.655082953301914	23.2	12.4	32.4	8.0	37.0
295-299	21.39894345738001	21.4	11.0	31.0	8.0	37.0
300	15.163869356659548	13.0	8.0	20.0	7.0	29.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.19857643351859622
1101	2	-0.009107583099776662
1101	3	-0.015074923862691492
1101	4	-0.023971048689560348
1101	5	-0.010626630113002022
1101	6	0.0037798666542911974
1101	7	-0.0142240340498887
1101	8	0.0286440707525486
1101	9	0.030821441253522153
1101	10-14	0.25872699864576276
1101	15-19	0.15201056833802795
1101	20-24	0.024354993626914734
1101	25-29	0.042108869768902935
1101	30-34	0.11818424389051785
1101	35-39	-0.012520534063902744
1101	40-44	0.0617082021736266
1101	45-49	0.052556018670941285
1101	50-54	0.03837704119503371
1101	55-59	0.07632319236713414
1101	60-64	-0.029936861330732256
1101	65-69	-0.06716128953005551
1101	70-74	-0.017550680416555053
1101	75-79	0.005383979019512708
1101	80-84	-0.06831847268952629
1101	85-89	-0.08122003065506078
1101	90-94	0.11044170253175167
1101	95-99	0.29695643482348544
1101	100-104	-0.06490226287788659
1101	105-109	-0.033632929356684826
1101	110-114	0.10518826541134985
1101	115-119	0.0858255467047826
1101	120-124	0.016418123901175363
1101	125-129	-0.05356952904117662
1101	130-134	-0.08997326072599066
1101	135-139	0.05924834060192552
1101	140-144	-0.04057499310879109
1101	145-149	-0.025774751250310146
1101	150-154	-0.008777714343345622
1101	155-159	-0.14960232196769851
1101	160-164	-0.1033461329620593
1101	165-169	-0.20158827758547915
1101	170-174	-0.16989097203972392
1101	175-179	-0.16433529678370462
1101	180-184	-0.19692423570498363
1101	185-189	-0.05159935823958506
1101	190-194	-0.1182633738283343
1101	195-199	0.12884102915671747
1101	200-204	-0.0779276690102435
1101	205-209	0.0657265415316104
1101	210-214	-0.031210470538582058
1101	215-219	0.6389859631837282
1101	220-224	0.29598032044963674
1101	225-229	0.07449262024656633
1101	230-234	0.26016159306980313
1101	235-239	-0.710494214203294
1101	240-244	-0.24052188637465122
1101	245-249	0.1511577970432576
1101	250-254	-0.23809044884243846
1101	255-259	-0.4206292404680383
1101	260-264	-1.478624820237819
1101	265-269	-1.23245770313817
1101	270-274	-1.4916559203992819
1101	275-279	-1.4888712301531264
1101	280-284	-0.7586509894852007
1101	285-289	-0.5290758834710516
1101	290-294	-0.6898322665463859
1101	295-299	-0.0430987630903239
1101	300	-0.8150236044038337
1102	1	-0.04830362195436777
1102	2	-0.04714213672476575
1102	3	0.03171776884789068
1102	4	0.04871806412479884
1102	5	-0.05080104673353958
1102	6	0.054295267363087873
1102	7	-0.084475397818494
1102	8	-0.027440642255932346
1102	9	-0.05496569226710335
1102	10-14	0.19207988255870134
1102	15-19	0.13493672325381567
1102	20-24	0.03969936436143229
1102	25-29	0.046489819175128844
1102	30-34	0.09537195664044873
1102	35-39	-0.009457224312725998
1102	40-44	-0.0015087705058860479
1102	45-49	0.09010163427993945
1102	50-54	0.10736548379470179
1102	55-59	0.09058736465748751
1102	60-64	0.11910351177299106
1102	65-69	0.05728109959704142
1102	70-74	0.13895801409638864
1102	75-79	0.08265434791811543
1102	80-84	0.09809033455737648
1102	85-89	-0.004641335812422653
1102	90-94	0.29641277345813677
1102	95-99	0.41389423563778394
1102	100-104	0.02639657235874182
1102	105-109	0.03336019728421746
1102	110-114	0.1541366698121962
1102	115-119	0.22728463306802382
1102	120-124	0.25513278319183996
1102	125-129	0.26201083685543836
1102	130-134	0.10159627484362943
1102	135-139	0.20568235537128032
1102	140-144	0.1270561302571025
1102	145-149	0.10239451049550041
1102	150-154	0.22017894988817233
1102	155-159	0.10105236216029567
1102	160-164	0.10837322483032352
1102	165-169	0.07814598492097247
1102	170-174	0.176777996736611
1102	175-179	0.11603568992479296
1102	180-184	0.14644004885659712
1102	185-189	0.3876809953366944
1102	190-194	0.365810076195352
1102	195-199	0.522264746323458
1102	200-204	0.26612528182582906
1102	205-209	0.1490966912670686
1102	210-214	0.31245626958736494
1102	215-219	1.037365752280401
1102	220-224	0.821609452895558
1102	225-229	0.7748586441156178
1102	230-234	0.9896733086678609
1102	235-239	0.8193788400657596
1102	240-244	0.8908707916440761
1102	245-249	0.9821967715285993
1102	250-254	1.2771842801824747
1102	255-259	1.206499733780145
1102	260-264	1.0090956805115248
1102	265-269	1.0996629158401277
1102	270-274	0.11057082120741413
1102	275-279	0.2337881229771881
1102	280-284	0.20396685626072397
1102	285-289	0.1875206498294304
1102	290-294	0.5897457070071148
1102	295-299	0.8479457410855744
1102	300	0.040254386907149
1103	1	-0.09134969595334042
1103	2	-0.06858704492631773
1103	3	-5.922508521365444E-4
1103	4	0.05529303651929496
1103	5	0.010887137182805873
1103	6	0.008420930879140087
1103	7	-0.030626715359396428
1103	8	-0.06283067278982912
1103	9	0.07971157213695079
1103	10-14	0.2818356345744206
1103	15-19	0.12909396237886028
1103	20-24	0.06576417306740012
1103	25-29	0.02128419575361562
1103	30-34	0.09831249310170875
1103	35-39	0.06312674388942696
1103	40-44	0.06094241346370666
1103	45-49	0.041937140172592535
1103	50-54	0.1513055827347003
1103	55-59	0.14934126241639945
1103	60-64	0.14401606539249912
1103	65-69	0.1916437653747849
1103	70-74	0.16964631144416842
1103	75-79	0.13937689693092636
1103	80-84	0.1102278458715773
1103	85-89	0.07057030642018702
1103	90-94	0.33359247432321837
1103	95-99	0.49743799586686066
1103	100-104	0.1677292528825589
1103	105-109	0.02026268559962574
1103	110-114	0.1714144772982138
1103	115-119	0.27457107523304103
1103	120-124	0.3201130252869362
1103	125-129	0.23120065858289252
1103	130-134	0.2200670801374045
1103	135-139	0.17743051905516438
1103	140-144	0.26639461661869746
1103	145-149	0.26034570719719596
1103	150-154	0.2885460464368492
1103	155-159	0.16328513273479217
1103	160-164	0.1263901886179184
1103	165-169	0.1978059683923874
1103	170-174	0.18962538052147693
1103	175-179	0.1628215290512287
1103	180-184	0.17616441045750975
1103	185-189	0.257733706232365
1103	190-194	0.19723417320007286
1103	195-199	0.3317929872796199
1103	200-204	0.17898366151558776
1103	205-209	0.1392035020148228
1103	210-214	0.44341645515194017
1103	215-219	1.1619641702590542
1103	220-224	0.6192140818242748
1103	225-229	0.8204187292123564
1103	230-234	0.8715144398339589
1103	235-239	0.7369080486615474
1103	240-244	0.8887114274157213
1103	245-249	1.368348413159321
1103	250-254	1.0513553625730552
1103	255-259	1.0956017503133566
1103	260-264	1.2702086049823897
1103	265-269	1.0798891536896527
1103	270-274	0.6274604153774952
1103	275-279	0.06419064254411566
1103	280-284	0.060569607783374835
1103	285-289	0.4161737788009745
1103	290-294	0.611767147864196
1103	295-299	0.7549453869016887
1103	300	0.03507820936148143
2116	1	-0.041612531758261184
2116	2	-0.04149485193158853
2116	3	0.03617742597327833
2116	4	-0.023560938232947137
2116	5	-0.022332698425771014
2116	6	-0.14019181809767645
2116	7	-0.11005650686611546
2116	8	-0.03264041517429206
2116	9	0.031209224779829015
2116	10-14	0.24346031374543742
2116	15-19	0.129874004493729
2116	20-24	-0.03506971776079837
2116	25-29	0.07832185810807601
2116	30-34	0.034656906180124736
2116	35-39	-0.024071900251705358
2116	40-44	0.010052322449979556
2116	45-49	0.05611692903389809
2116	50-54	0.048229269231718774
2116	55-59	0.1226331824052096
2116	60-64	0.023614104734974717
2116	65-69	0.08826134897844184
2116	70-74	-0.028535295105143632
2116	75-79	-0.07198230102893177
2116	80-84	0.003692693125827873
2116	85-89	-0.11777350468133818
2116	90-94	0.20897391250713326
2116	95-99	0.2961233465477875
2116	100-104	-0.17685812841218507
2116	105-109	-0.21608537226070723
2116	110-114	-0.07977901241586238
2116	115-119	0.021866647058175204
2116	120-124	0.10889897194849851
2116	125-129	-0.12253512529800759
2116	130-134	-0.1175642348881496
2116	135-139	-0.02514234425022721
2116	140-144	-0.08438366189201929
2116	145-149	0.049087211034205325
2116	150-154	0.13411704855199247
2116	155-159	0.1792387546486367
2116	160-164	-0.07114870179289312
2116	165-169	0.009544164883756423
2116	170-174	0.038486033454752544
2116	175-179	0.14002565212258133
2116	180-184	0.13065484055353238
2116	185-189	-0.17954853274540739
2116	190-194	-0.1591869919308877
2116	195-199	0.26780260950384616
2116	200-204	0.1878298411374999
2116	205-209	0.2586875067130663
2116	210-214	0.24562407603818315
2116	215-219	0.9000040535790035
2116	220-224	0.3899720553725672
2116	225-229	0.3686039217567796
2116	230-234	0.5542321185546655
2116	235-239	0.37193575488272046
2116	240-244	0.4908017686260919
2116	245-249	0.9780452966356492
2116	250-254	1.1969984373110734
2116	255-259	0.6025371824140073
2116	260-264	0.6053338385129727
2116	265-269	0.8447671534333381
2116	270-274	0.39481945343725755
2116	275-279	-0.6530903854254753
2116	280-284	0.7615148996069507
2116	285-289	0.13060729151244388
2116	290-294	0.42110613727198754
2116	295-299	0.7300336552893185
2116	300	0.24388600119975656
1117	1	-0.0020853623960377377
1117	2	-0.024834269583223545
1117	3	-0.004369623581588655
1117	4	0.09767296151819238
1117	5	-0.03281042191020589
1117	6	-0.06294578174294685
1117	7	-0.053573426447051986
1117	8	0.06634919734155176
1117	9	0.060572457410373204
1117	10-14	0.2515224945018346
1117	15-19	0.12090603183742132
1117	20-24	0.05123927693207264
1117	25-29	0.0749595056039567
1117	30-34	0.12626412134174103
1117	35-39	0.09873146063588223
1117	40-44	0.08576159477527767
1117	45-49	0.15208821183281884
1117	50-54	0.041542652251379764
1117	55-59	0.11940859136808513
1117	60-64	0.01236448341786911
1117	65-69	-0.01664181074576021
1117	70-74	0.1825332029901574
1117	75-79	0.1060436023458351
1117	80-84	0.06626768991765175
1117	85-89	0.08445353160716706
1117	90-94	0.23241363644510216
1117	95-99	0.4747501618085508
1117	100-104	0.01584701810074307
1117	105-109	-0.05111053672774091
1117	110-114	-0.017553780913893036
1117	115-119	0.20703997395364837
1117	120-124	0.20736818480959585
1117	125-129	0.09144019248798685
1117	130-134	0.1424414021399798
1117	135-139	0.21416385595333765
1117	140-144	0.23599196809860956
1117	145-149	0.26998658070664305
1117	150-154	0.23879062536353501
1117	155-159	0.1757271556722131
1117	160-164	0.04360847530998768
1117	165-169	0.10530584633541196
1117	170-174	0.25760018796206197
1117	175-179	0.17803703516919
1117	180-184	0.05183740936247716
1117	185-189	-0.7969299163721288
1117	190-194	-0.17956977075136393
1117	195-199	-0.10470855761293762
1117	200-204	0.2620083263238868
1117	205-209	0.2851040151190958
1117	210-214	0.2721020526615163
1117	215-219	0.8974476437447976
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2108	195-199	0.009332316048201506
2108	200-204	0.19451951193724426
2108	205-209	-0.04131840320493296
2108	210-214	0.028681142978548735
2108	215-219	0.8460305840621487
2108	220-224	0.42952015750189076
2108	225-229	0.4199934800899001
2108	230-234	0.6161405587788167
2108	235-239	0.22119144674256574
2108	240-244	0.6957584970380388
2108	245-249	1.1249387140858218
2108	250-254	0.6049389318613301
2108	255-259	0.4356696676295577
2108	260-264	0.48067145371554076
2108	265-269	0.64955406822153
2108	270-274	1.02382093566845
2108	275-279	1.2139175121850485
2108	280-284	0.8229807196407428
2108	285-289	0.6754235658229213
2108	290-294	0.19442431270508465
2108	295-299	0.7828452171083988
2108	300	-0.22322263229949968
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	7.0
13	9.0
14	14.0
15	41.0
16	69.0
17	514.0
18	7536.0
19	13657.0
20	12267.0
21	7497.0
22	4271.0
23	3178.0
24	3154.0
25	4029.0
26	5455.0
27	7422.0
28	10468.0
29	14598.0
30	19957.0
31	27355.0
32	37695.0
33	53303.0
34	74103.0
35	118824.0
36	183763.0
37	84397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.95234312259672	25.464724481424717	12.779148277855715	23.80378411812285
2	39.83142608743294	27.845694026526026	19.550796371883393	12.772083514157643
3	39.47328582159597	19.95103686220683	26.09795799493356	14.47771932126364
4	39.79913002481318	19.427955990847526	19.226249778325016	21.546664206014277
5	34.836205616342966	20.005536467877675	30.033175553610743	15.125082362168623
6	34.305771623583624	20.15014785541168	30.218445377121412	15.325635143883284
7	16.020577205612017	49.61828649202763	19.256094800478095	15.105041501882255
8	27.737559888290225	26.295050484224667	30.494836234452112	15.472553393032989
9	21.644850003532383	20.072002918179944	30.765604116594552	27.51754296169312
10-14	25.16249677399821	35.43959410769872	21.73928715092498	17.658621967378092
15-19	22.583886859972058	32.629807824009525	22.35118219448862	22.435123121529795
20-24	23.274503556171357	32.03114839896595	26.526457540049282	18.16789050481341
25-29	19.145624964856406	26.25424786939703	35.36712981719564	19.23299734855093
30-34	18.732004677161925	28.668695743696137	31.133981080851175	21.46531849829076
35-39	20.984424358728514	28.03266516047827	27.325986940279677	23.656923540513535
40-44	19.914386598287443	29.12084062037276	28.37595500466419	22.58881777667561
45-49	20.86515961319698	30.076544551985844	27.21197030492385	21.846325529893324
50-54	22.96322141690324	27.570139406531013	27.646438854470194	21.820200322095555
55-59	20.089160201446692	25.24655304412017	31.40500848492538	23.259278269507757
60-64	20.95400262117151	25.39822919535225	31.28874265949425	22.35902552398199
65-69	20.176157720128664	29.111642009680168	28.842719616830287	21.86948065336088
70-74	19.89382669413754	30.12741085061197	27.79327059630931	22.185491858941177
75-79	19.495662379268236	28.231430124440767	30.136840147466128	22.13606734882487
80-84	22.53734592687537	27.299717553630927	26.513971651554318	23.64896486793938
85-89	20.23140705582461	29.846521613130655	25.640190143068676	24.281881187976058
90-94	19.561840471868543	31.400279418613202	28.04820764061403	20.989672468904228
95-99	21.521317564011806	30.30930688900968	26.249922503867595	21.919453043110916
100-104	21.62019542001462	29.42084797349416	26.47625446413767	22.482702142353546
105-109	23.314902470216254	28.663216947358862	23.99761816538179	24.024262417043094
110-114	20.63098432343353	29.035832769834325	28.355395100514286	21.977787806217858
115-119	21.928766996884296	29.83720765935728	26.299433521294496	21.93459182246393
120-124	19.1799395313899	29.3588510675723	28.01475814718642	23.44645125385138
125-129	20.299494162773357	26.56591253416319	30.19550081115045	22.939092491913005
130-134	23.655056036568382	29.085265666911003	26.119419339695277	21.140258956825342
135-139	22.36935518008809	28.875370035127744	25.374014465179933	23.381260319604237
140-144	21.253606273510165	29.65392750399015	25.907382389706786	23.185083832792902
145-149	19.906081896470933	28.72253212665247	28.782539364430786	22.588846612445806
150-154	22.59237760578192	27.409251616173762	26.05333524802958	23.94503553001474
155-159	20.702993211481022	28.2126373376041	29.28495343310128	21.79941601781359
160-164	23.384685034091092	29.046992212900257	26.850398582433538	20.717924170575113
165-169	19.220107759273223	26.953198103183034	28.74294785195139	25.08374628559235
170-174	20.4888528121364	30.028532994609154	26.622970862896008	22.85964333035844
175-179	19.887834588747012	30.295820484591022	27.453477796738905	22.36286712992306
180-184	19.58358264259649	29.4753764149352	26.88157005001564	24.05947089245267
185-189	21.83332059753483	27.73721385904787	27.98476894618236	22.44469659723494
190-194	19.30298176281714	29.515025598954992	27.74805610864165	23.433936529586223
195-199	19.89013571555243	30.313343896837146	27.14665728542943	22.649863102180994
200-204	21.845639770767093	28.40009873370562	27.356849102947766	22.397412392579515
205-209	20.48481580430893	28.330452159294563	28.515404789333072	22.669327247063435
210-214	22.111239750685932	28.064326836153715	27.75667800393031	22.067755409230042
215-219	21.416736401191034	27.724764800972572	28.83664358458716	22.021855213249232
220-224	18.705245082419843	27.333282390139317	28.11738465331474	25.8440878741261
225-229	20.405373257418365	29.079576633221983	26.856800123417095	23.65824998594256
230-234	20.288992088906447	27.77103821748803	28.25493127715068	23.685038416454844
235-239	20.703333270855833	27.565958219852565	26.226421351157686	25.50428715813392
240-244	22.044888643464443	30.758308666734912	24.56031938499069	22.63648330480995
245-249	20.328093393292512	29.050740863025766	25.690104861278318	24.931060882403404
250-254	20.166555408653323	28.424572113215003	26.04002693260936	25.368845545522312
255-259	20.32636324708074	28.214763049267354	27.47405862023723	23.984815083414674
260-264	20.04195604563549	29.18168409548677	27.194265142023376	23.582094716854364
265-269	19.84962145842675	28.912098479922378	26.83811454432995	24.400165517320925
270-274	18.907816368048238	30.556112246119067	25.546358546850197	24.9897128389825
275-279	19.303298956289296	29.355880983242095	24.820504539471123	26.52031552099749
280-284	16.874265048095193	30.40078271837191	27.365326821830067	25.35962541170283
285-289	20.356064090382837	27.48299770899806	27.500904722289903	24.6600334783292
290-294	18.93414342623738	30.223664651526928	26.72871163220552	24.113480290030175
295-299	18.39174258884661	30.826216905546993	24.400799904265245	26.381240601341148
300	16.861284085682605	31.602850704241597	24.47854114071423	27.05732406936156
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.25
21	0.25
22	0.75
23	1.75
24	2.75
25	2.25
26	2.5
27	4.25
28	7.25
29	14.5
30	35.0
31	62.25
32	107.5
33	188.75
34	315.5
35	502.25
36	814.25
37	1612.25
38	4161.5
39	7090.25
40	12512.0
41	17901.5
42	27739.5
43	32759.0
44	32675.0
45	25772.0
46	15970.25
47	14257.75
48	12945.25
49	9844.25
50	9480.5
51	3350.5
52	713.0
53	168.75
54	85.5
55	59.75
56	43.0
57	29.0
58	18.5
59	14.25
60	10.75
61	7.75
62	4.5
63	4.0
64	3.0
65	2.25
66	3.5
67	2.25
68	1.75
69	1.5
70	0.75
71	1.0
72	0.5
73	0.75
74	1.25
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.25
81	0.25
82	0.0
83	0.0
84	0.0
85	0.0
86	0.25
87	0.0
88	0.0
89	0.25
90	0.25
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	2.8835770196213E-5
130-134	2.8835770196213E-5
135-139	2.8835770196213E-5
140-144	0.0
145-149	0.0
150-154	5.7671540392426E-5
155-159	2.8835770196213E-5
160-164	0.0
165-169	0.0
170-174	0.0
175-179	2.8835770196213E-5
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	2.8835770196213E-5
205-209	0.0
210-214	0.0
215-219	2.8835770196213E-5
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-259	0.0
260-264	0.0
265-269	0.0
270-274	0.0
275-279	0.0
280-284	2.8835770196213E-5
285-289	0.0
290-294	0.0
295-299	0.0
300	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
300	693583.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.461574501434333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	45.522614895340745	3.3967038254168926
2	4.430918863227186	0.6612326241556075
3	3.11223938323409	0.6966661807289759
4	2.5272801649245547	0.7542995694632725
5	1.8679484843480074	0.6968918390401999
6	1.9825358580512895	0.8875703403968841
7	1.9015188669193444	0.9931827283981176
8	1.831670654137775	1.0933717638351972
9	2.0957008328809352	1.4073505107533175
>10	32.74120629072287	50.69802401883492
>50	1.7134999214354258	8.282093387069589
>100	0.21403727029778377	2.4606137168214337
>500	0.00588287147895057	0.32219252487240296
>1k	0.027453296371249134	5.654887468405842
>5k	0.015687597926070433	8.584022021996173
>10k+	0.00980474870379402	13.410897479811156
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT	36670	5.2870384654756535	TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC	17252	2.487373537125333	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA	13909	2.005383638295633	No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	13672	1.9712132506131206	No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT	10153	1.463847874010753	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG	9683	1.3960838140496523	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA	8105	1.1685695872015316	No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	7573	1.0918664384796053	No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	7456	1.0749975129148206	No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	7349	1.0595703758598467	No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG	6601	0.95172459532601	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT	6335	0.9133730209650467	No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	5565	0.8023553057096267	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	4799	0.691914305858131	No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	4437	0.6397215618029853	No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT	4018	0.5793106232419192	No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT	3662	0.52798295229266	No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA	3515	0.5067886611984435	No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	3327	0.47968303721400324	No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA	2674	0.3855342475233678	No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT	2583	0.3724139720840909	No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	2123	0.306091700632801	No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT	1908	0.275093247671872	No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG	1876	0.2704795244404779	No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	1553	0.22390975557359394	No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG	1140	0.1643638901184141	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT	1033	0.14893675306344015	TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTAT	881	0.12702156771431825	TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA	785	0.11318039802013602	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.003892828976488755	0.0	0.0
2	0.007064763698072184	0.0	0.0
3	0.0115343080784852	0.0	0.0
4	0.01643638901184141	0.0	0.0
5	0.02076175454127336	0.0	0.0
6	0.025375477772667437	0.0	0.0
7	0.03373785112956921	0.0	0.0
8	0.04152350908254672	0.0	0.0
9	0.05204856520416446	0.0	0.0
10-14	0.07713568527486978	0.0	0.0
15-19	0.11179628105071782	0.0	0.0
20-24	0.14683174183911657	0.0	0.0
25-29	0.1674781532996051	0.0	0.0
30-34	0.19957236552799015	0.0	0.0
35-39	0.21384607177511555	0.0	0.0
40-44	0.22711052606537357	0.0	0.0
45-49	0.24175909732504977	0.0	0.0
50-54	0.2555714312490358	0.0	0.0
55-59	0.2709408967636173	0.0	0.0
60-64	0.28893441736605424	0.0	0.0
65-69	0.3092924711245806	0.0	0.0
70-74	0.3318420434180192	0.0	0.0
75-79	0.3612256932479602	0.0	0.0
80-84	0.39392545665046574	0.0	0.0
85-89	0.42919160360043423	0.0	0.0
90-94	0.4704267549810188	0.0	0.0
95-99	0.5229078567381265	0.0	0.0
100-104	0.58158864908742	0.0	0.0
105-109	0.6483434570916531	0.0	0.0
110-114	0.7270651097273145	0.0	0.0
115-119	0.8230882244807038	0.0	0.0
120-124	0.9372778744577073	0.0	0.0
125-129	1.0710181766277431	0.0	0.0
130-134	1.225722083730426	0.0	0.0
135-139	1.4068972278732323	0.0	0.0
140-144	1.61996473385305	0.0	0.0
145-149	1.872421901920895	0.0	0.0
150-154	2.1537725117253452	0.0	0.0
155-159	2.481491040005306	0.0	0.0
160-164	2.8704567441820226	0.0	0.0
165-169	3.3098562104319162	0.0	0.0
170-174	3.8402325316508623	0.0	0.0
175-179	4.4292608094489045	0.0	0.0
180-184	5.348170298291625	0.0	0.0
185-189	6.8672098364579295	0.0	0.0
190-194	7.640642864660753	0.0	0.0
195-199	8.514251358525224	0.0	0.0
200-204	9.454931853866084	0.0	0.0
205-209	10.451813265319363	0.0	0.0
210-214	11.502271537797208	0.0	0.0
215-219	12.637160945409562	0.0	0.0
220-224	13.862681178748613	0.0	0.0
225-229	15.183474796815954	0.0	0.0
230-234	16.52898066994145	0.0	0.0
235-239	17.92171953464834	0.0	0.0
240-244	19.230229114612094	0.0	0.0
245-249	20.51950523585497	0.0	0.0
250-254	21.873258139256585	0.0	1.1534308078485199E-4
255-259	23.241947971619837	0.0	1.4417885098106498E-4
260-264	24.60968622356661	0.0	1.4417885098106498E-4
265-269	26.000752613602124	0.0	1.4417885098106498E-4
270-274	27.37650720966344	0.0	1.4417885098106498E-4
275-279	28.70618224495122	0.0	1.4417885098106498E-4
280-284	30.003099845296095	0.0	1.4417885098106498E-4
285-288	31.147786205832613	0.0	1.4417885098106498E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	7555	0.0	288.55145	1
GGGTTAT	7630	0.0	285.52246	2
GGGGGGA	8615	0.0	282.56714	1
GTTATTA	7820	0.0	278.39722	4
GGTTATT	7905	0.0	275.58963	3
GGGGAGC	8845	0.0	274.38843	3
TTATTAG	8090	0.0	269.28754	5
GGGAGCG	5995	0.0	266.29144	4
GGAGCGA	6040	0.0	262.36044	5
TTAGTAG	8410	0.0	256.2445	8
GGGGGAG	9690	0.0	250.91599	2
GGAGCGT	65	0.0	248.76878	5
CGAAAGC	6470	0.0	245.15105	9
TATTAGT	8810	0.0	245.11082	6
ATTAGTA	8900	0.0	242.63217	7
GGGAGCA	3455	0.0	239.53938	4
GCGAAAG	6820	0.0	232.56998	8
TAGTAGA	9345	0.0	230.76364	9
GAGCGAA	6990	0.0	226.49316	6
AGCGAAA	7010	0.0	226.47606	7
>>END_MODULE