FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool b3.3410000000b6de.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool b3.3410000000b6de.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693583
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT366705.2870384654756535TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC172522.487373537125333No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA139092.005383638295633No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG136721.9712132506131206No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCTTAATTGTCGTACTCTT101531.463847874010753No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG96831.3960838140496523No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA81051.1685695872015316No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT75731.0918664384796053No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT74561.0749975129148206No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA73491.0595703758598467No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG66010.95172459532601No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT63350.9133730209650467No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA55650.8023553057096267No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA47990.691914305858131No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT44370.6397215618029853No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT40180.5793106232419192No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT36620.52798295229266No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA35150.5067886611984435No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG33270.47968303721400324No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA26740.3855342475233678No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT25830.3724139720840909No Hit
GGGGGGAGCGAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG21230.306091700632801No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT19080.275093247671872No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG18760.2704795244404779No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA15530.22390975557359394No Hit
GGGGGGAGCAAAAGCAGGGTACAAAGATTGGATCCTATGGATTTCCTTTG11400.1643638901184141No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCACCTCGTAT10330.14893675306344015TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAACTCGTAT8810.12702156771431825TruSeq Adapter, Index 8 (97% over 36bp)
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA7850.11318039802013602No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA75550.0288.551451
GGGTTAT76300.0285.522462
GGGGGGA86150.0282.567141
GTTATTA78200.0278.397224
GGTTATT79050.0275.589633
GGGGAGC88450.0274.388433
TTATTAG80900.0269.287545
GGGAGCG59950.0266.291444
GGAGCGA60400.0262.360445
TTAGTAG84100.0256.24458
GGGGGAG96900.0250.915992
GGAGCGT650.0248.768785
CGAAAGC64700.0245.151059
TATTAGT88100.0245.110826
ATTAGTA89000.0242.632177
GGGAGCA34550.0239.539384
GCGAAAG68200.0232.569988
TAGTAGA93450.0230.763649
GAGCGAA69900.0226.493166
AGCGAAA70100.0226.476067
Produced by FastQC (version 0.11.2)