FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool b1.3410000000b5a9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool b1.3410000000b5a9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences618638
Sequences flagged as poor quality0
Sequence length300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT6342410.25219918595366TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC106941.7286361329242625No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA96111.5535741419052822No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG77461.2521054316094389No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG68861.1130903694891034No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC59750.9658313908941901No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA51400.8308574643006087No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG50320.8133997588250317No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG44850.7249797134996557No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG42930.6939437926541855No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA42270.6832751948635551No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC37250.6021291934863361No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT37070.5992195759070733No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA35660.5764275715361811No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA35580.5751344081676198No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG35580.5751344081676198No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA34640.5599397385870251No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA28240.4564866691021244No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA27110.4382207365211966No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT25200.4073464610967965No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC24120.3898887556212195No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT17670.28562745903096803TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT15780.2550764744487083TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA15580.2518435660273051No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15280.24699420339520042No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14670.2371338327099208No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA13740.2221008085503962No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATATCGTAT7940.12834646432970492TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA58750.0291.748081
CGGGTTA49800.0289.867461
GGGTTAT50300.0287.278322
GGGGGAG61600.0278.252
GGTTATT54200.0266.6073
ATTAGTA54850.0262.91167
GTTATTA57700.0250.689794
GGAGCGA43350.0249.577855
TTAGTAG58200.0247.778378
GAGCGAA44300.0243.230266
TATTAGT59400.0242.772746
CGAAAGC44300.0241.902949
TTATTAG59750.0241.842685
GCGAAAG44450.0241.417318
GGGGAGC71500.0238.695113
TAGTAGA60600.0237.965359
GGGAGCG50600.0215.561254
AGCGAAA50650.0212.155987
GCAAAAG38250.0157.568638
GAGCAAA38500.0157.309086
Produced by FastQC (version 0.11.2)