FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool a3.3410000000b6b4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool a3.3410000000b6b4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences624617
Sequences flagged as poor quality0
Sequence length300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA56860.910317842774052No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA30950.4955036446334314No Hit
CGGGTTATTAGTAGAAACAAGGAAGGACTTGCCCCTCAGGGGAGGCATCA27730.44395205381858005No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA27660.4428313670617355No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA23840.38167388975964467No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC22210.35557789813597773No Hit
GGGGGGAGCGAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG19070.30530709218609164No Hit
GGGGGGAGCGAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT18430.2950608132663696No Hit
CGGGTTATTAGTAGAAACAAGGAAGGAATAAACATACCGGGACCGATACT17350.27777021758933873No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA16640.26640325191277214No Hit
CGGGTTATTAGTAGAAACAAGGACACCTACCTTAAGGATTTGGTGTGAGG16240.2599993275879459No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT16140.2583983465067393TruSeq Adapter, Index 8 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGGTATTTTTTCCTTAATTGTCGTACTCTT15380.24623089028956943No Hit
GGGGGGAGCGAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT14930.2390264754241399No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA14230.22781960785569397No Hit
GGGGGGAGCGAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT13840.22157578163898836No Hit
CGGGTTATTAGTAGAAACAAGGAGGATAGGAGAACATGCTAGGAGGGTAG13700.2193344081252992No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA13620.21805362326033392No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT13320.21325068001671424No Hit
GGGGGGAGCAAAAGCAGGATCCCTGATGCCTATGACAGTGGCTGCCACAT12890.206366461367526No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG12710.20348469542135422No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12640.20236400866450963No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT12030.19259802406914958No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT11970.19163743542042563No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA11910.19067684677170169No Hit
GGGGGGAGCGAAAGCAGGAGGATGAGTGACCACAATCCCTCCGGGACCCT11910.19067684677170169No Hit
GGGGGGAGCGAAAGCAGGTGCCTCTCCCAGGGTGGAGGAGAGGAGCGGGG11370.18203154893318627No Hit
CGGGTTATTAGTAGAAACAAGGACAAGTTTAGAAGCAAGGACAGTGTCAG10850.17370644731091212No Hit
CGGGTTATTAGTAGAAACAAGGTAAAATGTGTGAATATAAAAGCCACGCT10340.16554144379675864No Hit
GGGGGGAGCAAAAGCAGGTGCAGGATAAATGAAGGAACTGAACCAGCTAG10310.1650611494723967No Hit
GGGGGGAGCAAAAGCAGGAAAAAATGTACAATATTGAAAAGTGAAAATAT10090.16153899109374226No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG9990.1599380100125357No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAGGGAACAAGTTAA9020.144408493524832No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT8790.14072623703805692No Hit
CGGGTTATTAGTAGAAACAAGGGTCAGAAATGGGGTAGGAACTGTTAGCA8490.13592329379443724No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG8300.13288142974014477No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGTAAGAAGGAACCAGTTAA8120.12999966379397296No Hit
GGGGGGAGCAAAAGCAGGTGCCTCTCCCAGGGTGGAGGAGAGGAGCGGGG6690.10710563433271908No Hit
GGGGGGAGCGAAAGCAGGGAGTCAAGGATGGCCAAGGTTTGTGGCCTGAG6640.10630514379211581No Hit
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT6560.10502435892715055No Hit
CGGGTTATTAGTAGAAACAAGGCTGAAATTTTGGACTTGGAACCAAGAAA6550.10486426081902989No Hit
GGGGGGAGCAAAAGCAGGAGAGAAGGGACAACATTGATACAGTTATCAAT6540.10470416271090924No Hit
GGGGGGAGCAAAAGCAGGCCTGAGGACTGATCACCTGGGTGGATTTTCCA6530.10454406460278859No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6270.10038151379165151No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA41150.0288.641541
GGGTTAT43350.0273.314882
GGGGGGA96900.0264.417971
GGTTATT45450.0260.686463
TATTAGT47400.0248.101276
GGGAGCG61850.0247.653984
ATTAGTA47700.0246.849047
GGGGAGC103150.0246.686373
GGAGCGA62650.0241.675985
GGGGGAG107150.0238.574892
AGCGAAA64750.0232.929737
GCGAAAG64800.0232.523138
GAGCGAA65000.0232.260016
CGAAAGC66150.0227.777779
GTTATTA52600.0224.971484
TTATTAG52600.0224.412555
GAGCGTA750.0196.06
GAGCAAA52700.0191.072116
GCAAAAG53150.0187.518348
AGCAAAA55150.0181.517677
Produced by FastQC (version 0.11.2)