FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool a2.3410000000b64b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool a2.3410000000b64b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768284
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT10207113.285581894195374TruSeq Adapter, Index 6 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGAC263583.4307625825866475No Hit
GGGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG217232.8274700501377095No Hit
GGGGGGAGCAAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGG120051.5625732151131615No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTACGGATAACAAA118191.5383634176944982No Hit
GGGGGGAGCGAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA85611.1143014822643709No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTAATTAATGCACTCAAATGCA78401.0204559772167583No Hit
GGGGGGAGCGAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT70810.9216643845244726No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTTTCCTTAATTGTCGTACTCT50500.657309015936815No Hit
GGGGGGAGCGAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT50430.6563978945285858No Hit
GGGGGGAGCAAAAGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACA50060.6515819670850883No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTCTAAAATTGCGAAAGCTTAT46670.6074576588865576No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG45550.5928797163548896No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA41750.543418839908159No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT33070.43043978528773214No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACTATTCAGTATTAATTG33040.4300493046842053No Hit
GGGGGGAGCGAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA32360.42119841100426403No Hit
GGGGGGAGCAAAAGCAGGGGATAATTCTATTAACCATGAAGACTATCATT30360.3951663707691426No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT28520.37121689375283096No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAGACATAATGGATTCCAACACTGTG23650.3078288757803104No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAACCTCGTAT23130.3010605453191788TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGGTTAATAATCACTCACTGAGTGACATCAAAAT23100.30067006471565205No Hit
GGGGGGAGCGAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA20260.2637045675817796No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAAACTCGTAT20070.26123152375944314TruSeq Adapter, Index 6 (97% over 36bp)
GGGGGGAGCAAAAGCAGGAGTAAAGATGAATCCAAATCAAAAGATAATAA19690.25628543611477006No Hit
GGGGGGAGCGAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA16370.21307224932446853No Hit
GGGGGGAGCAAAAGCAGGTGGGGAGGTAAGAGAGGAGTAGGGGAGGGCCA11990.1560620812095527No Hit
GGGGGGAGCAAAAGCAGGTCAATTATATTCAGTATGGAAAGAATAAAAGA11530.1500747119554748No Hit
GGGGGAGCGAAAGCAGGTAGATATTGAAAGATGAGCCTTCTAACCGAGGT10080.13120148278501179No Hit
GGGGGGAGCGAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA9170.11935690447803156No Hit
GGGGGGAGCAAAAGCAGGACAGGCAAGGCTGGCAAGAAGGAACCAGTTAA8210.10686152516517329No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA73000.0292.589051
GGGTTAT73550.0290.201232
GGTTATT73850.0288.82333
GGGGAGC108700.0276.01253
GGGGGGA109650.0273.755221
TTATTAG78450.0271.700445
GGGAGCG73450.0269.582124
GGAGCGA73200.0267.69145
GTTATTA82050.0259.958564
TTAGTAG83450.0253.65978
GGGGGAG118500.0253.434272
TATTAGT84200.0252.447746
ATTAGTA86450.0245.537327
TAGTAGA87800.0241.259699
GAGCGAA81600.0239.954796
CGAAAGC82000.0238.246449
AGCGAAA83400.0234.599627
GCGAAAG83850.0232.989978
GGGAGCA44450.0230.172164
GCAAAAG49100.0205.080528
Produced by FastQC (version 0.11.2)