FastQCFastQC Report
Tue 12 May 2015
000000000-AE77A l01n01 clinical flu pool a1.3410000000b572.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE77A l01n01 clinical flu pool a1.3410000000b572.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434829
Sequences flagged as poor quality0
Sequence length300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT165493.805863914320342TruSeq Adapter, Index 7 (97% over 36bp)
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG47181.0850242279148814No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG42550.978545589185634No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG38740.8909249383090824No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC34180.7860561278111626No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAATATGGATAGCAAA34000.7819165695020341No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCTTCAATTGTCATACTCCTC31500.7244227040974728No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA31470.723732777712618No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA31470.723732777712618No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA27740.6379519305290126No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT25430.5848275988951979No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC24280.5583804208090997No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG23240.5344629728008022No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTCAAATT21740.49996665355806535No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG20330.4675401134698927No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA18560.4268344567634633No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA15840.3642811312033006No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13100.3012678547199014No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAGTA12270.282179891405587No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT11710.2693012655549653No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11160.2566526151659618No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG11010.2532029832416881No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9740.22399609961617095No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA6180.14212483528007563No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGACCTCGTAT4940.11360787803941319TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGAACTCGTAT4600.10578871234439284TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA23600.0292.753451
GGGTTAT23800.0290.293332
GGGGGGA37400.0288.88961
GGGGGAG39350.0273.826452
ATTAGTA24750.0273.80537
GGTTATT25750.0267.168183
GGAGCGA28400.0251.555665
GTTATTA27300.0250.383934
GCGTAAG600.0244.999338
GAGCGAA29150.0244.07486
GCGAAAG29500.0240.68078
CGAAAGC30100.0235.883099
GGGGAGC46200.0232.27213
GGAGCGT700.0230.999365
TTAGTAG29700.0226.686258
TATTAGT30200.0224.393436
TAGTAGA30900.0218.834349
AGCGAAA33350.0213.336767
GGGAGCG34350.0212.689394
GAGCGTA700.0209.999426
Produced by FastQC (version 0.11.2)