##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n02 flu mrt-pcr updated h7.3420000000876a.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74
Sequences flagged as poor quality	0
Sequence length	251
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.06756756756757	0.0	0.0	0.0	0.0	0.0
2	25.2972972972973	0.0	0.0	0.0	0.0	0.0
3	24.527027027027028	0.0	0.0	0.0	0.0	0.0
4	25.594594594594593	0.0	0.0	0.0	0.0	0.0
5	25.243243243243242	0.0	0.0	0.0	0.0	0.0
6	29.175675675675677	0.0	0.0	0.0	0.0	0.0
7	25.35135135135135	0.0	0.0	0.0	0.0	0.0
8	27.175675675675677	0.0	0.0	0.0	0.0	0.0
9	26.16216216216216	0.0	0.0	0.0	0.0	0.0
10-14	26.62972972972973	0.0	0.0	0.0	0.0	0.0
15-19	28.175675675675677	0.0	0.0	0.0	0.0	0.0
20-24	28.078378378378375	0.0	0.0	0.0	0.0	0.0
25-29	27.405405405405407	0.0	0.0	0.0	0.0	0.0
30-34	26.87837837837838	0.0	0.0	0.0	0.0	0.0
35-39	27.445945945945947	0.0	0.0	0.0	0.0	0.0
40-44	27.07297297297297	0.0	0.0	0.0	0.0	0.0
45-49	26.38378378378378	0.0	0.0	0.0	0.0	0.0
50-54	27.018918918918917	0.0	0.0	0.0	0.0	0.0
55-59	26.472972972972975	0.0	0.0	0.0	0.0	0.0
60-64	25.63783783783784	0.0	0.0	0.0	0.0	0.0
65-69	26.405405405405407	0.0	0.0	0.0	0.0	0.0
70-74	27.04864864864865	0.0	0.0	0.0	0.0	0.0
75-79	29.232432432432432	0.0	0.0	0.0	0.0	0.0
80-84	28.283783783783786	0.0	0.0	0.0	0.0	0.0
85-89	26.778378378378374	0.0	0.0	0.0	0.0	0.0
90-94	26.710810810810813	0.0	0.0	0.0	0.0	0.0
95-99	27.089189189189188	0.0	0.0	0.0	0.0	0.0
100-104	26.502702702702702	0.0	0.0	0.0	0.0	0.0
105-109	26.95405405405405	0.0	0.0	0.0	0.0	0.0
110-114	25.656756756756756	0.0	0.0	0.0	0.0	0.0
115-119	27.005405405405405	0.0	0.0	0.0	0.0	0.0
120-124	25.52972972972973	0.0	0.0	0.0	0.0	0.0
125-129	25.681081081081082	0.0	0.0	0.0	0.0	0.0
130-134	25.213513513513515	0.0	0.0	0.0	0.0	0.0
135-139	25.494594594594595	0.0	0.0	0.0	0.0	0.0
140-144	24.31621621621622	0.0	0.0	0.0	0.0	0.0
145-149	25.00810810810811	0.0	0.0	0.0	0.0	0.0
150-154	25.972972972972975	0.0	0.0	0.0	0.0	0.0
155-159	25.16756756756757	0.0	0.0	0.0	0.0	0.0
160-164	25.178378378378376	0.0	0.0	0.0	0.0	0.0
165-169	24.281081081081084	0.0	0.0	0.0	0.0	0.0
170-174	23.808108108108108	0.0	0.0	0.0	0.0	0.0
175-179	23.15945945945946	0.0	0.0	0.0	0.0	0.0
180-184	22.18918918918919	0.0	0.0	0.0	0.0	0.0
185-189	23.124324324324323	0.0	0.0	0.0	0.0	0.0
190-194	22.840540540540538	0.0	0.0	0.0	0.0	0.0
195-199	22.667567567567566	0.0	0.0	0.0	0.0	0.0
200-204	22.135135135135133	0.0	0.0	0.0	0.0	0.0
205-209	22.232432432432432	0.0	0.0	0.0	0.0	0.0
210-214	21.756756756756758	0.0	0.0	0.0	0.0	0.0
215-219	20.891891891891895	0.0	0.0	0.0	0.0	0.0
220-224	20.83783783783783	0.0	0.0	0.0	0.0	0.0
225-229	20.845945945945946	0.0	0.0	0.0	0.0	0.0
230-234	19.454054054054055	0.0	0.0	0.0	0.0	0.0
235-239	19.856756756756756	0.0	0.0	0.0	0.0	0.0
240-244	19.037837837837838	0.0	0.0	0.0	0.0	0.0
245-249	18.924324324324324	0.0	0.0	0.0	0.0	0.0
250-251	17.844594594594597	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1103	1	3.8571428571428577
1103	2	7.142857142857142
1103	3	4.571428571428573
1103	4	5.142857142857142
1103	5	2.8571428571428577
1103	6	7.142857142857142
1103	7	9.285714285714285
1103	8	4.0
1103	9	5.428571428571427
1103	10-14	5.571428571428573
1103	15-19	5.428571428571431
1103	20-24	6.9999999999999964
1103	25-29	6.5142857142857125
1103	30-34	6.342857142857142
1103	35-39	8.085714285714285
1103	40-44	6.714285714285719
1103	45-49	7.600000000000001
1103	50-54	8.942857142857147
1103	55-59	-0.2571428571428598
1103	60-64	4.342857142857142
1103	65-69	-0.9714285714285715
1103	70-74	6.771428571428576
1103	75-79	7.9714285714285715
1103	80-84	4.228571428571428
1103	85-89	6.5142857142857125
1103	90-94	8.428571428571427
1103	95-99	9.74285714285714
1103	100-104	8.31428571428571
1103	105-109	7.885714285714286
1103	110-114	5.599999999999998
1103	115-119	7.800000000000001
1103	120-124	7.657142857142848
1103	125-129	8.428571428571427
1103	130-134	4.914285714285715
1103	135-139	8.085714285714282
1103	140-144	9.457142857142863
1103	145-149	8.857142857142854
1103	150-154	6.085714285714285
1103	155-159	7.314285714285713
1103	160-164	7.257142857142856
1103	165-169	-1.7142857142857189
1103	170-174	-0.4857142857142911
1103	175-179	9.885714285714279
1103	180-184	6.199999999999999
1103	185-189	6.400000000000002
1103	190-194	8.742857142857144
1103	195-199	10.257142857142856
1103	200-204	7.74285714285714
1103	205-209	-2.2285714285714278
1103	210-214	-3.1142857142857174
1103	215-219	3.2285714285714278
1103	220-224	0.8571428571428541
1103	225-229	-0.2571428571428598
1103	230-234	5.828571428571429
1103	235-239	-1.1714285714285673
1103	240-244	-1.5714285714285694
1103	245-249	-8.657142857142858
1103	250-251	-7.214285714285715
2113	1	2.8571428571428577
2113	2	-7.857142857142858
2113	3	4.571428571428573
2113	4	5.142857142857142
2113	5	3.8571428571428577
2113	6	11.142857142857142
2113	7	5.285714285714285
2113	8	4.0
2113	9	1.428571428571427
2113	10-14	6.571428571428573
2113	15-19	5.428571428571431
2113	20-24	7.599999999999998
2113	25-29	8.314285714285717
2113	30-34	8.142857142857146
2113	35-39	8.085714285714285
2113	40-44	7.914285714285715
2113	45-49	7.600000000000001
2113	50-54	3.9428571428571466
2113	55-59	4.542857142857137
2113	60-64	8.542857142857137
2113	65-69	4.0285714285714285
2113	70-74	5.9714285714285715
2113	75-79	6.771428571428569
2113	80-84	7.228571428571428
2113	85-89	2.114285714285714
2113	90-94	-7.371428571428577
2113	95-99	2.5428571428571445
2113	100-104	-6.885714285714286
2113	105-109	3.4857142857142875
2113	110-114	7.599999999999998
2113	115-119	-3.0
2113	120-124	5.257142857142849
2113	125-129	0.6285714285714263
2113	130-134	4.714285714285719
2113	135-139	5.085714285714282
2113	140-144	-2.9428571428571395
2113	145-149	-2.942857142857143
2113	150-154	1.8857142857142897
2113	155-159	2.9142857142857146
2113	160-164	7.657142857142855
2113	165-169	-0.11428571428571743
2113	170-174	0.7142857142857117
2113	175-179	2.6857142857142833
2113	180-184	9.8
2113	185-189	8.8
2113	190-194	2.1428571428571423
2113	195-199	-5.5428571428571445
2113	200-204	-1.6571428571428584
2113	205-209	6.571428571428569
2113	210-214	2.8857142857142826
2113	215-219	5.428571428571431
2113	220-224	-1.5428571428571445
2113	225-229	0.14285714285714235
2113	230-234	4.228571428571431
2113	235-239	-3.771428571428565
2113	240-244	-1.5714285714285694
2113	245-249	-8.057142857142857
2113	250-251	-0.7142857142857153
2102	1	3.8571428571428577
2102	2	8.142857142857142
2102	3	6.571428571428573
2102	4	7.142857142857142
2102	5	2.8571428571428577
2102	6	7.142857142857142
2102	7	5.285714285714285
2102	8	4.0
2102	9	5.428571428571427
2102	10-14	6.571428571428573
2102	15-19	5.428571428571431
2102	20-24	7.599999999999998
2102	25-29	8.314285714285717
2102	30-34	8.342857142857142
2102	35-39	8.085714285714285
2102	40-44	7.714285714285719
2102	45-49	8.600000000000001
2102	50-54	8.942857142857147
2102	55-59	9.542857142857137
2102	60-64	9.942857142857143
2102	65-69	9.62857142857143
2102	70-74	8.171428571428574
2102	75-79	8.971428571428572
2102	80-84	9.628571428571426
2102	85-89	8.31428571428571
2102	90-94	9.228571428571424
2102	95-99	10.74285714285714
2102	100-104	9.514285714285712
2102	105-109	10.085714285714282
2102	110-114	9.999999999999996
2102	115-119	9.8
2102	120-124	9.25714285714285
2102	125-129	10.028571428571428
2102	130-134	10.114285714285717
2102	135-139	9.085714285714282
2102	140-144	11.25714285714286
2102	145-149	9.857142857142854
2102	150-154	8.285714285714288
2102	155-159	7.914285714285715
2102	160-164	9.657142857142855
2102	165-169	8.885714285714279
2102	170-174	8.11428571428571
2102	175-179	7.885714285714279
2102	180-184	11.000000000000004
2102	185-189	10.599999999999998
2102	190-194	10.542857142857141
2102	195-199	11.457142857142852
2102	200-204	8.74285714285714
2102	205-209	10.971428571428575
2102	210-214	10.885714285714283
2102	215-219	9.228571428571428
2102	220-224	12.657142857142858
2102	225-229	13.142857142857142
2102	230-234	1.4285714285714306
2102	235-239	1.8285714285714327
2102	240-244	12.428571428571434
2102	245-249	8.742857142857144
2102	250-251	3.7857142857142847
1114	1	-13.142857142857142
1114	2	-9.857142857142858
1114	3	-11.428571428571427
1114	4	-10.857142857142858
1114	5	-13.142857142857142
1114	6	-9.857142857142858
1114	7	1.2857142857142847
1114	8	-1.0
1114	9	-15.571428571428573
1114	10-14	-10.628571428571426
1114	15-19	-9.57142857142857
1114	20-24	-7.199999999999999
1114	25-29	-14.085714285714285
1114	30-34	-2.4571428571428555
1114	35-39	-11.914285714285715
1114	40-44	-11.685714285714283
1114	45-49	-10.799999999999997
1114	50-54	-13.457142857142852
1114	55-59	-9.857142857142861
1114	60-64	-13.65714285714286
1114	65-69	-7.171428571428571
1114	70-74	-7.228571428571428
1114	75-79	-8.428571428571427
1114	80-84	-8.571428571428573
1114	85-89	-8.285714285714288
1114	90-94	-1.5714285714285765
1114	95-99	-12.057142857142857
1114	100-104	-6.685714285714287
1114	105-109	-9.514285714285716
1114	110-114	-13.0
1114	115-119	-10.600000000000001
1114	120-124	-11.74285714285715
1114	125-129	-11.771428571428572
1114	130-134	-8.685714285714283
1114	135-139	-13.114285714285717
1114	140-144	-5.342857142857142
1114	145-149	-9.542857142857144
1114	150-154	-11.314285714285713
1114	155-159	-9.285714285714288
1114	160-164	-13.542857142857144
1114	165-169	-6.714285714285719
1114	170-174	-7.285714285714288
1114	175-179	-1.5142857142857196
1114	180-184	-12.199999999999998
1114	185-189	-6.400000000000002
1114	190-194	3.1428571428571423
1114	195-199	-3.7428571428571438
1114	200-204	3.3428571428571416
1114	205-209	2.9714285714285715
1114	210-214	8.885714285714283
1114	215-219	5.828571428571429
1114	220-224	1.057142857142857
1114	225-229	1.9428571428571395
1114	230-234	3.828571428571429
1114	235-239	6.428571428571434
1114	240-244	-3.1714285714285673
1114	245-249	10.542857142857141
1114	250-251	7.785714285714285
1108	1	-0.14285714285714235
1108	2	6.142857142857142
1108	3	4.571428571428573
1108	4	2.1428571428571423
1108	5	2.8571428571428577
1108	6	6.142857142857142
1108	7	4.285714285714285
1108	8	4.0
1108	9	5.428571428571427
1108	10-14	6.571428571428573
1108	15-19	5.428571428571431
1108	20-24	7.599999999999998
1108	25-29	9.514285714285712
1108	30-34	7.742857142857147
1108	35-39	7.885714285714283
1108	40-44	7.314285714285713
1108	45-49	7.0
1108	50-54	8.942857142857147
1108	55-59	9.342857142857142
1108	60-64	10.342857142857142
1108	65-69	9.62857142857143
1108	70-74	7.9714285714285715
1108	75-79	7.571428571428573
1108	80-84	8.428571428571423
1108	85-89	7.914285714285711
1108	90-94	8.228571428571424
1108	95-99	9.942857142857143
1108	100-104	8.114285714285714
1108	105-109	8.485714285714288
1108	110-114	9.8
1108	115-119	9.599999999999998
1108	120-124	8.457142857142852
1108	125-129	9.62857142857143
1108	130-134	9.914285714285715
1108	135-139	9.285714285714285
1108	140-144	9.057142857142857
1108	145-149	9.057142857142857
1108	150-154	6.685714285714287
1108	155-159	5.314285714285713
1108	160-164	9.057142857142853
1108	165-169	6.48571428571428
1108	170-174	7.514285714285709
1108	175-179	8.285714285714285
1108	180-184	9.600000000000005
1108	185-189	9.8
1108	190-194	5.9428571428571395
1108	195-199	9.857142857142858
1108	200-204	6.542857142857137
1108	205-209	3.171428571428571
1108	210-214	2.8857142857142826
1108	215-219	1.8285714285714292
1108	220-224	5.857142857142854
1108	225-229	2.1428571428571423
1108	230-234	-3.7714285714285687
1108	235-239	10.228571428571431
1108	240-244	10.228571428571431
1108	245-249	6.742857142857144
1108	250-251	3.2857142857142847
2105	1	2.8571428571428577
2105	2	6.142857142857142
2105	3	2.571428571428573
2105	4	-10.857142857142858
2105	5	0.8571428571428577
2105	6	-11.857142857142858
2105	7	-13.714285714285715
2105	8	2.0
2105	9	0.42857142857142705
2105	10-14	0.17142857142857437
2105	15-19	0.4285714285714306
2105	20-24	-15.200000000000001
2105	25-29	-9.085714285714285
2105	30-34	-14.457142857142856
2105	35-39	-11.914285714285715
2105	40-44	-5.885714285714283
2105	45-49	-8.799999999999997
2105	50-54	-10.657142857142851
2105	55-59	-7.857142857142861
2105	60-64	-6.457142857142859
2105	65-69	0.2285714285714313
2105	70-74	-9.628571428571426
2105	75-79	-9.028571428571428
2105	80-84	-7.371428571428574
2105	85-89	-5.685714285714287
2105	90-94	-9.971428571428575
2105	95-99	-11.257142857142856
2105	100-104	-9.285714285714288
2105	105-109	-9.314285714285717
2105	110-114	-12.400000000000002
2105	115-119	-5.800000000000001
2105	120-124	-12.94285714285715
2105	125-129	-7.371428571428574
2105	130-134	-8.885714285714283
2105	135-139	-3.914285714285718
2105	140-144	-9.342857142857142
2105	145-149	-4.942857142857143
2105	150-154	-0.11428571428571388
2105	155-159	-2.885714285714286
2105	160-164	-5.342857142857145
2105	165-169	2.2857142857142847
2105	170-174	4.514285714285709
2105	175-179	-11.114285714285717
2105	180-184	-9.2
2105	185-189	-14.600000000000001
2105	190-194	-15.657142857142858
2105	195-199	-10.742857142857146
2105	200-204	-7.25714285714286
2105	205-209	-10.82857142857143
2105	210-214	-12.114285714285717
2105	215-219	-9.57142857142857
2105	220-224	-7.742857142857144
2105	225-229	-6.657142857142858
2105	230-234	-3.171428571428571
2105	235-239	-8.371428571428567
2105	240-244	-5.771428571428569
2105	245-249	-5.457142857142859
2105	250-251	-6.214285714285715
2109	1	-0.14285714285714235
2109	2	-9.857142857142858
2109	3	-11.428571428571427
2109	4	2.1428571428571423
2109	5	-0.14285714285714235
2109	6	-9.857142857142858
2109	7	-11.714285714285715
2109	8	-17.0
2109	9	-2.571428571428573
2109	10-14	-14.828571428571426
2109	15-19	-12.57142857142857
2109	20-24	-7.400000000000002
2109	25-29	-9.485714285714284
2109	30-34	-13.657142857142855
2109	35-39	-8.314285714285713
2109	40-44	-12.085714285714285
2109	45-49	-11.2
2109	50-54	-6.657142857142851
2109	55-59	-5.457142857142859
2109	60-64	-13.05714285714286
2109	65-69	-15.37142857142857
2109	70-74	-12.028571428571428
2109	75-79	-13.82857142857143
2109	80-84	-13.571428571428573
2109	85-89	-10.885714285714286
2109	90-94	-6.971428571428575
2109	95-99	-9.657142857142858
2109	100-104	-3.085714285714289
2109	105-109	-11.114285714285714
2109	110-114	-7.600000000000001
2109	115-119	-7.800000000000001
2109	120-124	-5.94285714285715
2109	125-129	-9.571428571428573
2109	130-134	-12.085714285714285
2109	135-139	-14.514285714285716
2109	140-144	-12.14285714285714
2109	145-149	-10.342857142857142
2109	150-154	-11.514285714285712
2109	155-159	-11.285714285714288
2109	160-164	-14.742857142857146
2109	165-169	-9.114285714285717
2109	170-174	-13.085714285714289
2109	175-179	-16.114285714285717
2109	180-184	-15.199999999999998
2109	185-189	-14.600000000000001
2109	190-194	-14.85714285714286
2109	195-199	-11.542857142857144
2109	200-204	-17.45714285714286
2109	205-209	-10.628571428571426
2109	210-214	-10.314285714285717
2109	215-219	-15.971428571428572
2109	220-224	-11.142857142857144
2109	225-229	-10.457142857142859
2109	230-234	-8.37142857142857
2109	235-239	-5.171428571428567
2109	240-244	-10.57142857142857
2109	245-249	-3.8571428571428577
2109	250-251	-0.7142857142857153
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
15	5.0
16	11.0
17	5.0
18	6.0
19	2.0
20	1.0
21	4.0
22	2.0
23	1.0
24	3.0
25	2.0
26	1.0
27	3.0
28	2.0
29	3.0
30	2.0
31	4.0
32	1.0
33	4.0
34	3.0
35	1.0
36	7.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	60.810810810810814	20.27027027027027	2.7027027027027026	16.216216216216218
2	40.54054054054054	35.13513513513514	14.864864864864865	9.45945945945946
3	31.08108108108108	18.91891891891892	40.54054054054054	9.45945945945946
4	33.78378378378378	14.864864864864865	24.324324324324326	27.027027027027028
5	54.054054054054056	9.45945945945946	29.72972972972973	6.756756756756757
6	55.4054054054054	9.45945945945946	13.513513513513514	21.62162162162162
7	29.72972972972973	44.5945945945946	16.216216216216218	9.45945945945946
8	21.62162162162162	47.2972972972973	16.216216216216218	14.864864864864865
9	24.324324324324326	29.72972972972973	20.27027027027027	25.675675675675674
10-14	37.2972972972973	31.08108108108108	19.45945945945946	12.162162162162163
15-19	36.21621621621622	23.513513513513516	18.64864864864865	21.62162162162162
20-24	43.513513513513516	25.945945945945947	17.83783783783784	12.702702702702704
25-29	24.054054054054056	43.513513513513516	18.37837837837838	14.054054054054054
30-34	38.37837837837838	23.243243243243246	24.864864864864867	13.513513513513514
35-39	31.351351351351354	28.91891891891892	21.62162162162162	18.10810810810811
40-44	32.7027027027027	27.297297297297295	24.054054054054056	15.945945945945947
45-49	20.81081081081081	39.729729729729726	21.35135135135135	18.10810810810811
50-54	29.18918918918919	21.62162162162162	27.56756756756757	21.62162162162162
55-59	44.32432432432433	19.72972972972973	18.91891891891892	17.027027027027028
60-64	39.729729729729726	22.432432432432435	15.945945945945947	21.891891891891895
65-69	25.405405405405407	24.054054054054056	31.351351351351354	19.18918918918919
70-74	19.18918918918919	45.67567567567568	21.08108108108108	14.054054054054054
75-79	14.864864864864865	51.35135135135135	17.027027027027028	16.756756756756758
80-84	17.2972972972973	44.5945945945946	18.91891891891892	19.18918918918919
85-89	17.56756756756757	39.45945945945946	23.783783783783786	19.18918918918919
90-94	18.64864864864865	36.75675675675676	22.432432432432435	22.162162162162165
95-99	23.513513513513516	31.351351351351354	22.702702702702705	22.432432432432435
100-104	19.45945945945946	34.86486486486486	19.72972972972973	25.945945945945947
105-109	27.027027027027028	23.513513513513516	24.054054054054056	25.405405405405407
110-114	19.18918918918919	27.297297297297295	30.54054054054054	22.972972972972975
115-119	18.37837837837838	31.891891891891895	24.594594594594597	25.135135135135133
120-124	12.972972972972974	31.62162162162162	28.10810810810811	27.297297297297295
125-129	19.45945945945946	30.0	25.405405405405407	25.135135135135133
130-134	24.324324324324326	32.7027027027027	22.162162162162165	20.81081081081081
135-139	21.62162162162162	29.18918918918919	21.62162162162162	27.56756756756757
140-144	26.216216216216214	25.675675675675674	21.891891891891895	26.216216216216214
145-149	20.81081081081081	28.91891891891892	25.405405405405407	24.864864864864867
150-154	23.513513513513516	30.0	20.0	26.486486486486488
155-159	24.864864864864867	28.91891891891892	26.486486486486488	19.72972972972973
160-164	22.702702702702705	31.08108108108108	19.72972972972973	26.486486486486488
165-169	25.135135135135133	27.837837837837835	24.864864864864867	22.162162162162165
170-174	27.837837837837835	27.027027027027028	22.972972972972975	22.162162162162165
175-179	23.243243243243246	30.270270270270274	24.594594594594597	21.891891891891895
180-184	24.864864864864867	26.486486486486488	24.324324324324326	24.324324324324326
185-189	21.891891891891895	26.486486486486488	28.91891891891892	22.702702702702705
190-194	22.972972972972975	29.72972972972973	26.756756756756754	20.54054054054054
195-199	22.702702702702705	28.10810810810811	26.486486486486488	22.702702702702705
200-204	21.35135135135135	30.810810810810814	25.945945945945947	21.891891891891895
205-209	22.702702702702705	33.24324324324324	23.243243243243246	20.81081081081081
210-214	21.891891891891895	33.78378378378378	21.35135135135135	22.972972972972975
215-219	22.162162162162165	31.351351351351354	23.783783783783786	22.702702702702705
220-224	24.594594594594597	32.16216216216216	24.054054054054056	19.18918918918919
225-229	26.216216216216214	29.45945945945946	25.135135135135133	19.18918918918919
230-234	22.432432432432435	29.45945945945946	26.486486486486488	21.62162162162162
235-239	25.135135135135133	31.891891891891895	23.243243243243246	19.72972972972973
240-244	24.054054054054056	31.08108108108108	24.594594594594597	20.27027027027027
245-249	25.135135135135133	29.72972972972973	23.783783783783786	21.35135135135135
250-251	27.027027027027028	33.78378378378378	18.91891891891892	20.27027027027027
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.3333333333333333
35	0.3333333333333333
36	0.0
37	0.3333333333333333
38	1.0
39	0.6666666666666666
40	1.0
41	0.3333333333333333
42	1.9999999999999998
43	3.0
44	3.666666666666667
45	4.666666666666666
46	4.333333333333333
47	3.3333333333333335
48	3.0
49	1.9999999999999998
50	1.6666666666666665
51	1.3333333333333333
52	0.6666666666666666
53	1.3333333333333333
54	0.3333333333333333
55	0.0
56	0.0
57	1.0
58	0.3333333333333333
59	0.0
60	0.3333333333333333
61	0.3333333333333333
62	0.3333333333333333
63	0.6666666666666666
64	0.3333333333333333
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	74.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.64864864864865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.63013698630137	97.2972972972973
2	1.36986301369863	2.7027027027027026
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC	2	2.7027027027027026	No Hit
GGGGGGAGCAAAAGCAGGGGACGGGGCAAAAGGAGAAGCCGGTTCCCCGC	1	1.3513513513513513	No Hit
ACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGA	1	1.3513513513513513	No Hit
GGTCGGGAGAGGGGCGGGGAGGGGAAAAGGGTGTGGGTAAGTGTAACTCC	1	1.3513513513513513	No Hit
AGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGATTTGGAGTAACAG	1	1.3513513513513513	No Hit
GTCCTCGAGATTTTTCCGTACCAGAACATATAGGAGTCTGGGTGCATTTT	1	1.3513513513513513	No Hit
GATAAGAAAAGCGCTCGGTGGGGGAACAATTTAGGACAACGTGGAAATTG	1	1.3513513513513513	No Hit
GAAGTTCGGAGAGGCGTGTGGGGGAAGAGTGTAAGATGCTGTGGGGATAT	1	1.3513513513513513	No Hit
GGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAA	1	1.3513513513513513	No Hit
ATCTTCTCCCCAACCCTGCAAAGGAAACCAAGCATTGACAAAATATTTCT	1	1.3513513513513513	No Hit
GATCGGAAAAGCGGCGGGGTGGGGAAAAGGGGGTGAGTTCGATTAGATAT	1	1.3513513513513513	No Hit
TAGACGTGAAGATCTAAGAATTTGAGAATAAATTAAACAAGAAGAATGGT	1	1.3513513513513513	No Hit
ATTGTTTGCTTGGCGCCAAACTCTCCTTATTTCTTCTTTGTCATAAAGGA	1	1.3513513513513513	No Hit
CCCCCAGGGACAGTGGAGATAGCAGGCAAGGTATGCTGGGTGAGCGAGGG	1	1.3513513513513513	No Hit
GGTTTGAAAAGGGGCGGGGAGGGGAAAAGGGGTGGAGGACAGGTTAAATT	1	1.3513513513513513	No Hit
GGTTTGGATAACGAGGCCAAGGGGAAAGGCTCTTCCTTTTAAGGACTTTT	1	1.3513513513513513	No Hit
CATCTGGATCTTCAGTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG	1	1.3513513513513513	No Hit
GTTGGGATGAGGGGAGAGTAGGGGGAGAGTTGATCGAGCTCTGTGTGGAC	1	1.3513513513513513	No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGTGGGGGCGCAAGGGTAGGACT	1	1.3513513513513513	No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1	1.3513513513513513	No Hit
AGGAAGGATCTTATATCCAGAATACATAAAGAACTCCTACAACCCAAAAA	1	1.3513513513513513	No Hit
GAAGTCTTCAAGGTCAGCTTATGAAAGCACTTATTCAAGATCCTGCTAAG	1	1.3513513513513513	No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA	1	1.3513513513513513	No Hit
CCATGAGCCATCTCCTCCAGGAAGCCTGCCCAGACTTTCCCACCCTCAAC	1	1.3513513513513513	No Hit
CTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGGATG	1	1.3513513513513513	No Hit
GGTTGGAAAAGGGGCGGGGAGGGGAAGGGAGGGGGGGGGAGCGGTGGACT	1	1.3513513513513513	No Hit
GGTCGCCCACGAGATACTCTCTTTGTCGATTCCGCTTCTTTACTGATAAA	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGCCAGGTTGTGGTCTTTGTGAGGTCTGGTCTAT	1	1.3513513513513513	No Hit
GGATGCAAAAAGGTCGGGGAAGGGAAAAGGGGGGGGAAAAATGTTTAGAA	1	1.3513513513513513	No Hit
AGGGGAGGCTAATATGCTAATTTGGCAAGGAAACGTAATGGTGGCAATTA	1	1.3513513513513513	No Hit
GGATCGCAGGGCGGGGGGAGCGGGAAGAGAGATCCTGAAATGCTCGTATT	1	1.3513513513513513	No Hit
GGGGTGAAAATGGACGAAGGACAAGGGTTGCTTATGAAAGAATGTGCAAT	1	1.3513513513513513	No Hit
ATAATCACTGAGTTTGAGTTCAGTGCACATTTGGATGTAGAATCTCCCGA	1	1.3513513513513513	No Hit
CATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCATGAGGACATGCTGC	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA	1	1.3513513513513513	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGGAGTACGTGGAGAAGT	1	1.3513513513513513	Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGGGTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGG	1	1.3513513513513513	No Hit
GATTGGAAGAGCCGCGGGTGGGGGAAGAGGGTGTCAGGACGGGTGGGAAT	1	1.3513513513513513	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	1	1.3513513513513513	No Hit
GAATTGAAGAGGGGCGGGTAGGGGAAAAGGGGGGGAGGTAGGGTAGAGAA	1	1.3513513513513513	No Hit
GATCGGAGAGAAGGCGGGTAGGGGGAAAGGGGAGGCGAAAGTGTAGATTT	1	1.3513513513513513	No Hit
GTGGGCAGAAGATATAATAAGAATGGAAAAAGAAAAGCCAAAAAAATTGT	1	1.3513513513513513	No Hit
GATGGGTACTCACTGGTCGGGAGAGACCCATTCAAAGTACTCAAAAACAG	1	1.3513513513513513	No Hit
AATGATCTCTGTGCGTTGGTTAATGCCTCTTGGTTCAACTCCTTCCTCAC	1	1.3513513513513513	No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA	1	1.3513513513513513	No Hit
ATGTGCACTGAACTCAAACTCAGTGATTATGAGGGACGACTAATCCAGAA	1	1.3513513513513513	No Hit
AAACCCTTTTACTCCATTTGCTCCATTAGACGATACTGGACCACAACTGC	1	1.3513513513513513	No Hit
TATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCA	1	1.3513513513513513	No Hit
CATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACC	1	1.3513513513513513	No Hit
GATCGGAAGGGCGGCGGGTAGGGGAAAAGGGGTGGGGAAAGGGTTGAAAT	1	1.3513513513513513	No Hit
AAACAGCACTTTGTAACTCTTCCATATCAAGGAACAGATGTTTTCACCTG	1	1.3513513513513513	No Hit
AGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTT	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGGAGACAAAAACATAATGGACTCCAACACCATG	1	1.3513513513513513	No Hit
GATCGGGAAAGGGGCGGGTTGGGGAAGAGGGTGGGCGGTAGGGGAGATAT	1	1.3513513513513513	No Hit
GGGAGCAAAAGCAGGAACAGAGGCAGGCCTGAAGCCAGGCCTTGAGGCCC	1	1.3513513513513513	No Hit
GATTGGGAGAAGGGCGGGGAGGGGAAAAGGGTGGGCGATAGGGTTGAAGT	1	1.3513513513513513	No Hit
GGGGGGAGCAAAAGCAGGTTAGGTATATTCGATATGGAGCGAATTAAAGG	1	1.3513513513513513	No Hit
GGACGGGAGAGCGGCGGGTAGGGGAAAAGGGGGGGGGAAAGGGTAAAGTT	1	1.3513513513513513	No Hit
GCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAATTGAAG	1	1.3513513513513513	No Hit
GTCTACGCTGCAGTCCTCGCTCACTGGGCACGGCGAGCGTGAACACAAAT	1	1.3513513513513513	No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGGGGGGATAGGGGAGAAAT	1	1.3513513513513513	No Hit
GATTGGGAAAAGGGGCTGGCGGGGGAAAGGGGGGCCACAGGTGTGATAAA	1	1.3513513513513513	No Hit
GGTCGGGAGAACGGCGGGTTGGGGAAAAGGGGGGCTGGAAAGGGGAATAT	1	1.3513513513513513	No Hit
GGAATGCCAATGGATAAGAAAGAAAGAAAGAGAAAAAAGAGAACAAAAGG	1	1.3513513513513513	No Hit
GGGGGGAGCAAAAGCAGGAACCAGCCAGCCGGATGACCGTAGTTGCAGGC	1	1.3513513513513513	No Hit
GTGGTCCAGTATCGTCTAATGGGGCAAATGGCGTAAAAGGGTTTTCATTC	1	1.3513513513513513	No Hit
ATGTAGTCATCGCCAGGAACATCCGAGGATTTTGATTTTCATTCCACTTA	1	1.3513513513513513	No Hit
GGTCGGGAAAGAGGCGTGTAGGGGAAAAGGGTGGCAGGACGTGAACGGTA	1	1.3513513513513513	No Hit
GAAAGGGAAAGGGGCGGGTAGGGGAAAAGGGGGGGGGAAAGGGGAAAAAT	1	1.3513513513513513	No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAAAGGGGAGAGCATAACGGAATTTC	1	1.3513513513513513	No Hit
GGGGGGAGCGAAAGCAGGGTCTCCTGGGACTGGGGCAGAGAGCAGGACAG	1	1.3513513513513513	No Hit
AATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCAT	1	1.3513513513513513	No Hit
ACTCTCATCTTTACTTGTAAAAGCTTCCTCCAGACATTTGCAAATCCTTT	1	1.3513513513513513	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.5405405405405406	0.0	0.0
80-84	1.3513513513513513	0.0	0.0
85-89	1.3513513513513513	0.0	0.0
90-94	1.3513513513513513	0.0	0.0
95-99	1.3513513513513513	0.0	0.0
100-104	1.3513513513513513	0.0	0.0
105-109	1.3513513513513513	0.0	0.0
110-114	1.3513513513513513	0.0	0.0
115-119	1.3513513513513513	0.0	0.0
120-124	1.3513513513513513	0.0	0.0
125-129	1.8918918918918919	0.0	0.0
130-134	2.7027027027027026	0.0	0.0
135-139	5.135135135135135	0.0	0.0
140-144	6.756756756756756	0.0	0.0
145-149	7.5675675675675675	0.0	0.0
150-154	8.91891891891892	0.0	0.0
155-159	9.45945945945946	0.0	0.0
160-164	10.27027027027027	0.0	0.0
165-169	11.35135135135135	0.0	0.0
170-174	13.513513513513512	0.0	0.0
175-179	13.783783783783784	0.0	0.0
180-184	14.864864864864865	0.0	0.0
185-189	16.486486486486488	0.0	0.0
190-194	18.64864864864865	0.0	0.0
195-199	19.18918918918919	0.0	0.0
200-204	20.27027027027027	0.0	0.0
205-209	23.513513513513512	0.0	0.0
210-214	25.675675675675677	0.0	0.0
215-219	25.675675675675677	0.0	0.0
220-224	25.675675675675677	0.0	0.0
225-229	26.75675675675676	0.0	0.0
230-234	27.027027027027025	0.0	0.0
235-239	27.83783783783784	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCATT	5	0.0	245.00002	5
CCCAGCA	5	0.0	245.00002	3
GCCCAGC	5	0.0	245.00002	2
CCAGCAT	5	0.0	245.00002	4
CATTAAG	5	0.0	245.00002	8
GGCCCAG	5	0.0	245.00002	1
ATTAAGC	5	0.0	245.00002	9
AGCATTA	5	0.0	245.00002	6
GTCAGTA	5	0.0	245.00002	245
GCATTAA	5	0.0	245.00002	7
>>END_MODULE