FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated h7.3420000000876a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated h7.3420000000876a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC22.7027027027027026No Hit
GGGGGGAGCAAAAGCAGGGGACGGGGCAAAAGGAGAAGCCGGTTCCCCGC11.3513513513513513No Hit
ACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAGAGCAATTGGGA11.3513513513513513No Hit
GGTCGGGAGAGGGGCGGGGAGGGGAAAAGGGTGTGGGTAAGTGTAACTCC11.3513513513513513No Hit
AGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGATTTGGAGTAACAG11.3513513513513513No Hit
GTCCTCGAGATTTTTCCGTACCAGAACATATAGGAGTCTGGGTGCATTTT11.3513513513513513No Hit
GATAAGAAAAGCGCTCGGTGGGGGAACAATTTAGGACAACGTGGAAATTG11.3513513513513513No Hit
GAAGTTCGGAGAGGCGTGTGGGGGAAGAGTGTAAGATGCTGTGGGGATAT11.3513513513513513No Hit
GGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAA11.3513513513513513No Hit
ATCTTCTCCCCAACCCTGCAAAGGAAACCAAGCATTGACAAAATATTTCT11.3513513513513513No Hit
GATCGGAAAAGCGGCGGGGTGGGGAAAAGGGGGTGAGTTCGATTAGATAT11.3513513513513513No Hit
TAGACGTGAAGATCTAAGAATTTGAGAATAAATTAAACAAGAAGAATGGT11.3513513513513513No Hit
ATTGTTTGCTTGGCGCCAAACTCTCCTTATTTCTTCTTTGTCATAAAGGA11.3513513513513513No Hit
CCCCCAGGGACAGTGGAGATAGCAGGCAAGGTATGCTGGGTGAGCGAGGG11.3513513513513513No Hit
GGTTTGAAAAGGGGCGGGGAGGGGAAAAGGGGTGGAGGACAGGTTAAATT11.3513513513513513No Hit
GGTTTGGATAACGAGGCCAAGGGGAAAGGCTCTTCCTTTTAAGGACTTTT11.3513513513513513No Hit
CATCTGGATCTTCAGTCATTGCCATCATCCACTTCATTCTGAGTGCGGGG11.3513513513513513No Hit
GTTGGGATGAGGGGAGAGTAGGGGGAGAGTTGATCGAGCTCTGTGTGGAC11.3513513513513513No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGTGGGGGCGCAAGGGTAGGACT11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11.3513513513513513No Hit
AGGAAGGATCTTATATCCAGAATACATAAAGAACTCCTACAACCCAAAAA11.3513513513513513No Hit
GAAGTCTTCAAGGTCAGCTTATGAAAGCACTTATTCAAGATCCTGCTAAG11.3513513513513513No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA11.3513513513513513No Hit
CCATGAGCCATCTCCTCCAGGAAGCCTGCCCAGACTTTCCCACCCTCAAC11.3513513513513513No Hit
CTAGTCTTGCCATTTTGTTTGAGAACATTATAGGTGCCATGCTCAGGATG11.3513513513513513No Hit
GGTTGGAAAAGGGGCGGGGAGGGGAAGGGAGGGGGGGGGAGCGGTGGACT11.3513513513513513No Hit
GGTCGCCCACGAGATACTCTCTTTGTCGATTCCGCTTCTTTACTGATAAA11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGCCAGGTTGTGGTCTTTGTGAGGTCTGGTCTAT11.3513513513513513No Hit
GGATGCAAAAAGGTCGGGGAAGGGAAAAGGGGGGGGAAAAATGTTTAGAA11.3513513513513513No Hit
AGGGGAGGCTAATATGCTAATTTGGCAAGGAAACGTAATGGTGGCAATTA11.3513513513513513No Hit
GGATCGCAGGGCGGGGGGAGCGGGAAGAGAGATCCTGAAATGCTCGTATT11.3513513513513513No Hit
GGGGTGAAAATGGACGAAGGACAAGGGTTGCTTATGAAAGAATGTGCAAT11.3513513513513513No Hit
ATAATCACTGAGTTTGAGTTCAGTGCACATTTGGATGTAGAATCTCCCGA11.3513513513513513No Hit
CATATTAAATTTTTGTAGAAGCTTTTTGCTCCAGCATGAGGACATGCTGC11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA11.3513513513513513No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGGAGTACGTGGAGAAGT11.3513513513513513Illumina Single End PCR Primer 1 (97% over 34bp)
CCAGGGTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGG11.3513513513513513No Hit
GATTGGAAGAGCCGCGGGTGGGGGAAGAGGGTGTCAGGACGGGTGGGAAT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG11.3513513513513513No Hit
GAATTGAAGAGGGGCGGGTAGGGGAAAAGGGGGGGAGGTAGGGTAGAGAA11.3513513513513513No Hit
GATCGGAGAGAAGGCGGGTAGGGGGAAAGGGGAGGCGAAAGTGTAGATTT11.3513513513513513No Hit
GTGGGCAGAAGATATAATAAGAATGGAAAAAGAAAAGCCAAAAAAATTGT11.3513513513513513No Hit
GATGGGTACTCACTGGTCGGGAGAGACCCATTCAAAGTACTCAAAAACAG11.3513513513513513No Hit
AATGATCTCTGTGCGTTGGTTAATGCCTCTTGGTTCAACTCCTTCCTCAC11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA11.3513513513513513No Hit
ATGTGCACTGAACTCAAACTCAGTGATTATGAGGGACGACTAATCCAGAA11.3513513513513513No Hit
AAACCCTTTTACTCCATTTGCTCCATTAGACGATACTGGACCACAACTGC11.3513513513513513No Hit
TATTGAGAAGTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCA11.3513513513513513No Hit
CATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACC11.3513513513513513No Hit
GATCGGAAGGGCGGCGGGTAGGGGAAAAGGGGTGGGGAAAGGGTTGAAAT11.3513513513513513No Hit
AAACAGCACTTTGTAACTCTTCCATATCAAGGAACAGATGTTTTCACCTG11.3513513513513513No Hit
AGTAGGTACAGATGCAATTGTCATTCTAAGTGTCTCGCTGGATTCCTCTT11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGGAGACAAAAACATAATGGACTCCAACACCATG11.3513513513513513No Hit
GATCGGGAAAGGGGCGGGTTGGGGAAGAGGGTGGGCGGTAGGGGAGATAT11.3513513513513513No Hit
GGGAGCAAAAGCAGGAACAGAGGCAGGCCTGAAGCCAGGCCTTGAGGCCC11.3513513513513513No Hit
GATTGGGAGAAGGGCGGGGAGGGGAAAAGGGTGGGCGATAGGGTTGAAGT11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTTAGGTATATTCGATATGGAGCGAATTAAAGG11.3513513513513513No Hit
GGACGGGAGAGCGGCGGGTAGGGGAAAAGGGGGGGGGAAAGGGTAAAGTT11.3513513513513513No Hit
GCGAGAGCAATTGGGACAGAAATTTGAGGAAATAAGGTGGTTAATTGAAG11.3513513513513513No Hit
GTCTACGCTGCAGTCCTCGCTCACTGGGCACGGCGAGCGTGAACACAAAT11.3513513513513513No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGGGGGGATAGGGGAGAAAT11.3513513513513513No Hit
GATTGGGAAAAGGGGCTGGCGGGGGAAAGGGGGGCCACAGGTGTGATAAA11.3513513513513513No Hit
GGTCGGGAGAACGGCGGGTTGGGGAAAAGGGGGGCTGGAAAGGGGAATAT11.3513513513513513No Hit
GGAATGCCAATGGATAAGAAAGAAAGAAAGAGAAAAAAGAGAACAAAAGG11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGAACCAGCCAGCCGGATGACCGTAGTTGCAGGC11.3513513513513513No Hit
GTGGTCCAGTATCGTCTAATGGGGCAAATGGCGTAAAAGGGTTTTCATTC11.3513513513513513No Hit
ATGTAGTCATCGCCAGGAACATCCGAGGATTTTGATTTTCATTCCACTTA11.3513513513513513No Hit
GGTCGGGAAAGAGGCGTGTAGGGGAAAAGGGTGGCAGGACGTGAACGGTA11.3513513513513513No Hit
GAAAGGGAAAGGGGCGGGTAGGGGAAAAGGGGGGGGGAAAGGGGAAAAAT11.3513513513513513No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAAAGGGGAGAGCATAACGGAATTTC11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGGTCTCCTGGGACTGGGGCAGAGAGCAGGACAG11.3513513513513513No Hit
AATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCAT11.3513513513513513No Hit
ACTCTCATCTTTACTTGTAAAAGCTTCCTCCAGACATTTGCAAATCCTTT11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCATT50.0245.000025
CCCAGCA50.0245.000023
GCCCAGC50.0245.000022
CCAGCAT50.0245.000024
CATTAAG50.0245.000028
GGCCCAG50.0245.000021
ATTAAGC50.0245.000029
AGCATTA50.0245.000026
GTCAGTA50.0245.00002245
GCATTAA50.0245.000027