FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated h5.3420000000866b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated h5.3420000000866b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401344
Sequences flagged as poor quality0
Sequence length251
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA160443.997568170945623No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC150283.744418752989954No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC84632.1086648859830968No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG81532.031424413969064No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG75531.881926726199968No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG72341.8024437888693987No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA45561.1351857757933344No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT41881.0434938606282889No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA36340.9054576622548238No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG36140.900474405995854No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC35090.8743123106362622No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG33110.8249780736724605No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA31480.7843645351618562No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG30480.7594482538670069No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA20330.5065479987242865No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC15850.39492305852336146No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15090.37598668473927604No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA15010.37399338223568807No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11280.28105565300590013No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9840.2451762079413172No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9270.23097392760325305No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6080.15149099027268378No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5250.13081047679795885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA67700.0243.733381
GGGTTAT67850.0242.111282
TATTAGT68750.0239.298196
TTATTAG69000.0238.431155
ATTAGTA69550.0238.130837
GGTTATT69200.0237.565033
GTTATTA71600.0229.601944
TAGTAGA73600.0226.525159
TTAGTAG75600.0219.722218
GAGCGAA36250.0218.979316
GCGAAAG36650.0218.260578
AGCGAAA36400.0218.076927
GGAGCGA36550.0217.517095
CGAAAGC37250.0216.06049
GGGGGGA53000.0204.55191
GGGGGAG54900.0195.241362
GGGGAGC55850.0191.481643
AAACCCC3100.0189.67741245
GGGAGCG43250.0182.97114
CGAGAGG12550.0176.67331245
Produced by FastQC (version 0.11.2)