##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE52Y l01n02 flu mrt-pcr updated h4.342000000085e3.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17465 Sequences flagged as poor quality 0 Sequence length 251 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.58265101631835 33.0 32.0 33.0 29.0 34.0 2 31.645920412253076 33.0 32.0 34.0 29.0 34.0 3 31.79009447466361 33.0 32.0 34.0 30.0 34.0 4 31.739650730031492 33.0 32.0 34.0 29.0 34.0 5 31.755854566275406 33.0 32.0 34.0 29.0 34.0 6 33.687374749499 34.0 33.0 37.0 30.0 37.0 7 33.50609791010593 35.0 33.0 37.0 30.0 37.0 8 34.10180360721443 37.0 33.0 37.0 30.0 37.0 9 34.08972230174635 37.0 33.0 37.0 32.0 37.0 10-14 35.23301460062983 37.4 35.6 37.4 32.8 37.4 15-19 36.27464070999141 38.0 38.0 38.0 33.8 38.0 20-24 36.54557114228457 38.4 37.8 38.4 34.0 38.4 25-29 36.855367878614366 39.0 38.0 39.0 34.0 39.0 30-34 36.87316346979674 39.0 38.0 39.0 34.0 39.0 35-39 36.83864872602347 39.0 38.0 39.0 34.2 39.0 40-44 36.83594617807043 39.0 38.0 39.0 34.2 39.0 45-49 36.628834812482104 39.0 38.0 39.0 33.6 39.0 50-54 36.709292871457194 39.0 38.0 39.0 33.4 39.0 55-59 36.699513312338965 39.0 38.0 39.0 33.4 39.0 60-64 36.575551102204415 39.0 38.0 39.0 33.2 39.0 65-69 36.67437732608073 39.0 38.0 39.0 33.4 39.0 70-74 36.754755224735185 39.0 38.0 39.0 33.6 39.0 75-79 36.87997709705125 39.0 38.0 39.0 33.2 39.0 80-84 36.803412539364444 39.0 38.0 39.0 33.0 39.0 85-89 36.619902662467794 39.0 38.0 39.0 33.2 39.0 90-94 36.559530489550525 39.0 38.0 39.0 33.0 39.0 95-99 36.47821356999714 39.0 38.0 39.0 33.0 39.0 100-104 36.4866189521901 39.0 38.0 39.0 32.8 39.0 105-109 36.44782135699971 39.0 38.0 39.0 32.8 39.0 110-114 36.381173776123674 39.0 38.0 39.0 32.8 39.0 115-119 36.24663040366447 38.6 37.6 39.0 32.0 39.0 120-124 36.14152877182937 38.6 37.0 39.0 30.8 39.0 125-129 36.22148296593186 39.0 37.4 39.0 31.4 39.0 130-134 35.98154022330375 38.0 37.0 39.0 30.0 39.0 135-139 35.86148296593187 38.0 37.0 39.0 29.2 39.0 140-144 35.730501002004004 38.0 37.0 39.0 27.4 39.0 145-149 35.49777268823361 38.0 37.0 39.0 26.2 39.0 150-154 35.333684511880904 38.0 37.0 39.0 25.0 39.0 155-159 35.17305468079015 38.0 37.0 39.0 24.4 39.0 160-164 34.9621185227598 38.0 37.0 39.0 18.6 39.0 165-169 34.679209848267966 38.0 36.2 39.0 16.0 39.0 170-174 34.607683939307194 38.0 36.4 39.0 16.0 39.0 175-179 34.46946464357286 38.0 36.2 39.0 15.6 39.0 180-184 34.05174921271113 37.6 35.0 39.0 15.0 39.0 185-189 33.752407672487834 37.0 34.0 39.0 15.0 39.0 190-194 33.55632407672488 37.0 34.0 39.0 15.0 39.0 195-199 33.15527054108217 37.0 33.0 39.0 14.4 39.0 200-204 32.80483252218723 37.0 32.6 39.0 14.0 39.0 205-209 32.36377898654452 37.0 31.2 38.2 14.0 39.0 210-214 31.991880904666477 37.0 28.6 38.0 14.0 39.0 215-219 31.23334669338677 37.0 25.6 38.0 14.0 39.0 220-224 30.662857142857142 37.0 24.4 38.0 14.0 38.8 225-229 30.242244488977953 36.2 22.4 37.6 13.8 38.4 230-234 29.04170626968222 33.8 15.4 37.0 13.4 38.0 235-239 28.908090466647582 34.2 15.0 37.0 13.0 38.0 240-244 28.324374463212138 33.0 15.0 37.0 13.0 38.0 245-249 28.03967935871744 33.0 15.0 37.0 13.0 37.8 250-251 25.30741482965932 28.5 14.5 35.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3017980267846383 1101 2 -0.007084302907230722 1101 3 0.7183298643511016 1101 4 0.22001431507433722 1101 5 0.6233400260229836 1101 6 1.1024998496536185 1101 7 1.0258211284638676 1101 8 1.0068116645673726 1101 9 1.329386495108217 1101 10-14 0.8510519489673527 1101 15-19 0.7126489866038881 1101 20-24 0.7667063987732945 1101 25-29 0.24900217333790664 1101 30-34 0.725040376423074 1101 35-39 0.5722664092742491 1101 40-44 0.5720129640703036 1101 45-49 0.8394483273590438 1101 50-54 0.5401109185072457 1101 55-59 0.6131990016830855 1101 60-64 0.8752357626203491 1101 65-69 0.27605230348422083 1101 70-74 0.6503797381564951 1101 75-79 0.2891510676368796 1101 80-84 0.6178184364822172 1101 85-89 0.6367821148904866 1101 90-94 0.8227095972300091 1101 95-99 0.6242444315817508 1101 100-104 0.5790716672757981 1101 105-109 0.7585383658307165 1101 110-114 0.36672893133697926 1101 115-119 0.525249995220257 1101 120-124 0.6451401137274644 1101 125-129 0.9297436232300669 1101 130-134 0.6938299568775435 1101 135-139 0.7041952921566264 1101 140-144 0.38081272012382783 1101 145-149 0.648061439262932 1101 150-154 0.30740000010450785 1101 155-159 0.6503460440840954 1101 160-164 0.5443576325287509 1101 165-169 1.3158498282330768 1101 170-174 0.7936683470214305 1101 175-179 0.879161821397183 1101 180-184 0.949762309288829 1101 185-189 0.6376574502837755 1101 190-194 0.5077255092843771 1101 195-199 0.9105776974332755 1101 200-204 -0.08878719851531258 1101 205-209 0.7631394211802416 1101 210-214 -0.11332219196322768 1101 215-219 -0.11447757345601417 1101 220-224 0.93421559338584 1101 225-229 1.2852507590562716 1101 230-234 0.9067264989304391 1101 235-239 1.0362311829941007 1101 240-244 1.374610434229023 1101 245-249 0.0682423274937598 1101 250-251 0.3162044140207172 1102 1 -0.08781890869923359 1102 2 0.3008794067701892 1102 3 0.5056282514478774 1102 4 0.24672802475175715 1102 5 -0.029381748170564492 1102 6 0.9003829141697466 1102 7 0.6180590316896755 1102 8 1.2941100516641484 1102 9 0.2925921402695053 1102 10-14 0.5087132392899392 1102 15-19 0.29551188982969734 1102 20-24 0.1703354310313543 1102 25-29 0.35152233462822835 1102 30-34 -0.17495962357691752 1102 35-39 0.26873818346779643 1102 40-44 0.6738274801993285 1102 45-49 0.32977090800420683 1102 50-54 0.22498995076530548 1102 55-59 0.10039658232824422 1102 60-64 0.5663647948784103 1102 65-69 -0.08241543845127097 1102 70-74 0.3024966736403627 1102 75-79 0.6245341321530091 1102 80-84 0.12638698486931332 1102 85-89 0.0461571148904838 1102 90-94 0.24811282303645754 1102 95-99 0.49289362513012946 1102 100-104 0.4003418285661198 1102 105-109 0.14573594647587385 1102 110-114 0.38487409262729955 1102 115-119 0.46698386618800214 1102 120-124 0.3780030169532722 1102 125-129 0.3794412038752242 1102 130-134 0.06167269881302673 1102 135-139 0.5075219050598463 1102 140-144 0.6394820749625367 1102 145-149 0.7037066005532537 1102 150-154 0.5787709678464523 1102 155-159 0.7376444311808683 1102 160-164 0.9404261809158498 1102 165-169 1.3189748282330669 1102 170-174 1.3744143147633636 1102 175-179 0.9133352084939546 1102 180-184 0.9730485996114098 1102 185-189 1.1386655147999036 1102 190-194 1.9844392189617963 1102 195-199 1.8029164071107004 1102 200-204 2.6518378014846817 1102 205-209 1.4611232921479882 1102 210-214 2.301496356423872 1102 215-219 2.2051796846084954 1102 220-224 3.472421238547131 1102 225-229 2.0079322106691713 1102 230-234 3.4493676279626975 1102 235-239 2.113247312026356 1102 240-244 2.3752152729387 1102 245-249 2.0477786178163413 1102 250-251 2.1957407043432973 1103 1 0.6338912807528914 1103 2 0.2555645066165795 1103 3 0.42319097546221585 1103 4 0.23315915634417905 1103 5 0.5164451847531417 1103 6 0.8601883417171123 1103 7 0.6505731125908483 1103 8 0.6878632518689614 1103 9 0.3350908601875844 1103 10-14 0.2825995680149731 1103 15-19 0.16453390723880545 1103 20-24 -0.015634871067973677 1103 25-29 0.016908919369654996 1103 30-34 0.12980228118497905 1103 35-39 0.18957791721075523 1103 40-44 0.23481058311791259 1103 45-49 0.064845152755872 1103 50-54 0.007273616919938775 1103 55-59 0.32753431914339615 1103 60-64 0.2581722705568552 1103 65-69 0.4615681765000943 1103 70-74 0.26491346831523543 1103 75-79 -0.026771551410732286 1103 80-84 0.5220347063234883 1103 85-89 0.49060155933492666 1103 90-94 0.07489213691254548 1103 95-99 0.18342101888333673 1103 100-104 0.2546173021964293 1103 105-109 0.31573082614817594 1103 110-114 0.8074035345115789 1103 115-119 1.0965793602996214 1103 120-124 0.3136917010290503 1103 125-129 0.6891186232300655 1103 130-134 0.5653081314807125 1103 135-139 0.6328163239026594 1103 140-144 0.40968176774287457 1103 145-149 0.8754423916438867 1103 150-154 0.8377571429616566 1103 155-159 0.26473096471901414 1103 160-164 0.8265500928462117 1103 165-169 1.091990701248946 1103 170-174 0.6635592200372926 1103 175-179 1.4277233293336877 1103 180-184 0.8056650870666076 1103 185-189 1.044502688379005 1103 190-194 1.2375370172208875 1103 195-199 1.2449030942586745 1103 200-204 1.1994568491037327 1103 205-209 1.6065918021326198 1103 210-214 1.7320647127986781 1103 215-219 1.9094807598773187 1103 220-224 2.0704755140207602 1103 225-229 2.229744806675317 1103 230-234 2.727708641787583 1103 235-239 3.0646041988671158 1103 240-244 2.1352255135941007 1103 245-249 2.237537962414393 1103 250-251 1.7648651283064325 2112 1 -0.22007697321536313 2112 2 -1.2001131490610781 2112 3 -0.8109970587258211 2112 4 -0.48503376184874014 2112 5 -1.2115157432077872 2112 6 0.29120177273053827 2112 7 -0.25663079461305216 2112 8 0.30056166456737543 2112 9 -0.7069115818148575 2112 10-14 -0.868755743340337 2112 15-19 -1.1745625518576475 2112 20-24 -1.2473320627651674 2112 25-29 -1.1445074420467023 2112 30-34 -1.3164980851153842 2112 35-39 -1.4045124368795996 2112 40-44 -1.5657754974681595 2112 45-49 -1.5236285957178808 2112 50-54 -1.3619083122619955 2112 55-59 -1.0022336906246068 2112 60-64 -1.305148852764269 2112 65-69 -1.2576015426696259 2112 70-74 -1.0098606464588826 2112 75-79 -0.5677239323631227 2112 80-84 -0.38472964044086666 2112 85-89 -0.9015351928018163 2112 90-94 -1.51402117200076 2112 95-99 -1.3146497991874782 2112 100-104 -1.5095341019549693 2112 105-109 -1.2276635572462098 2112 110-114 -0.8819249148168709 2112 115-119 -0.8931153893951276 2112 120-124 -1.3320714247340746 2112 125-129 -0.9908813767699343 2112 130-134 -0.7877565815839915 2112 135-139 -0.672775861689523 2112 140-144 -0.5917834337223269 2112 145-149 -0.42770779150630034 2112 150-154 -0.57894615374164 2112 155-159 -1.0435962636082152 2112 160-164 -0.9168442905481768 2112 165-169 -0.20256363330538818 2112 170-174 -0.5140720375939551 2112 175-179 -0.49867471706436106 2112 180-184 -0.17826653686501714 2112 185-189 -0.6305156266393013 2112 190-194 -0.25193795225408167 2112 195-199 0.17115462051020813 2112 200-204 0.04722241686929607 2112 205-209 0.30256249810331326 2112 210-214 0.2273028080367645 2112 215-219 0.3607628111593719 2112 220-224 0.580609824155065 2112 225-229 0.43135652828703996 2112 230-234 1.9694668835458202 2112 235-239 1.0056542599171792 2112 240-244 0.7081681265367159 2112 245-249 0.796463481339913 2112 250-251 0.2505794140207165 1113 1 0.274659868889902 1113 2 -0.48378386431074105 1113 3 -0.16160434617521346 1113 4 -0.011619456855488153 1113 5 0.006837833040528096 1113 6 -0.9476646240305939 1113 7 -0.6317994855712215 1113 8 -0.8994383354326274 1113 9 -1.8370060487514337 1113 10-14 -1.0873252440150978 1113 15-19 -0.8894343467294448 1113 20-24 -0.7352672854372386 1113 25-29 -0.6729824757848988 1113 30-34 -0.6451350621734164 1113 35-39 -0.7709901696731194 1113 40-44 -0.6930089657542595 1113 45-49 -0.6903762340444644 1113 50-54 -0.34727943236995884 1113 55-59 -0.8039610860362103 1113 60-64 -0.979588798783162 1113 65-69 -0.909693310550864 1113 70-74 -0.3319119285101735 1113 75-79 -0.028776563942066957 1113 80-84 -0.49868375650024177 1113 85-89 -0.6240183237059966 1113 90-94 -0.41215882382262237 1113 95-99 -0.3337051298217588 1113 100-104 -0.5943384204434992 1113 105-109 -0.5270427745201616 1113 110-114 -1.0062973844524947 1113 115-119 -1.0755175486393895 1113 120-124 -0.1999256757462149 1113 125-129 -0.5880743592260771 1113 130-134 -0.04087399049087992 1113 135-139 -0.4059472517030187 1113 140-144 -0.49407763075335964 1113 145-149 -0.476938560737068 1113 150-154 -0.23141578936917284 1113 155-159 0.4739206054875993 1113 160-164 -0.12499543764668175 1113 165-169 0.22248360016290292 1113 170-174 0.6852801891266935 1113 175-179 0.33382190911648735 1113 180-184 -0.5580776029918653 1113 185-189 -0.39008377778639414 1113 190-194 -0.014259139838429746 1113 195-199 -0.41508019730356693 1113 200-204 0.2606097313092448 1113 205-209 -0.6199746139074804 1113 210-214 -0.13059192880533388 1113 215-219 -0.7571859067893527 1113 220-224 -0.7343699329299547 1113 225-229 -0.04243564445250314 1113 230-234 -0.3695344659818396 1113 235-239 -0.4179354836725686 1113 240-244 0.10147446931673798 1113 245-249 -0.5587861812781689 1113 250-251 -0.5020521649266527 2113 1 -1.497000050138439 2113 2 0.8306560817081561 2113 3 0.48131063358187376 2113 4 1.3918893150743372 2113 5 0.5423304106383675 2113 6 0.09120177273054253 2113 7 0.7433692053869478 2113 8 1.3928693568750674 2113 9 -0.3222961971994778 2113 10-14 0.5158596412750498 2113 15-19 0.4777451404500397 2113 20-24 0.3588217833886773 2113 25-29 0.732415634876368 2113 30-34 0.5450403764230813 2113 35-39 0.955487563120407 2113 40-44 1.2065321948395322 2113 45-49 1.1502175581282756 2113 50-54 0.9273224569687883 2113 55-59 1.0900740016830852 2113 60-64 0.9379280703126511 2113 65-69 0.6316292265611381 2113 70-74 0.7039855073872658 2113 75-79 0.6907376060984234 2113 80-84 0.8921934364822164 2113 85-89 0.6861571148904844 2113 90-94 1.0705942126146226 2113 95-99 1.042273277735596 2113 100-104 0.9027735903527159 2113 105-109 0.4984902889076466 2113 110-114 0.34576739287543745 2113 115-119 0.47611537983564034 2113 120-124 0.606390113727457 2113 125-129 0.47373400784545794 2113 130-134 0.8891664953390759 2113 135-139 1.2656856767720086 2113 140-144 1.331293489354593 2113 145-149 0.6553691315706232 2113 150-154 0.7872076924122027 2113 155-159 1.144096044084094 2113 160-164 0.8554634017595149 2113 165-169 0.47743636669461154 2113 170-174 0.8705433470214317 2113 175-179 0.224402206012563 2113 180-184 -0.10442038301886214 2113 185-189 -0.3197463958700695 2113 190-194 0.1634466631305287 2113 195-199 -0.08422999487441274 2113 200-204 0.31183780148468543 2113 205-209 0.31179326733408175 2113 210-214 -0.03423565350168545 2113 215-219 0.677685888082447 2113 220-224 0.4575329010781495 2113 225-229 0.23751037444088752 2113 230-234 0.966389960468895 2113 235-239 0.2702696445325614 2113 240-244 0.8804758188444062 2113 245-249 -0.4189211340447052 2113 250-251 0.2044255678668705 1112 1 -0.4046923578307471 1112 2 0.5383483894004613 1112 3 -0.087920135648897 1112 4 -0.10041837723335334 1112 5 0.6346381029460595 1112 6 -0.06264438111561077 1112 7 -0.05663079461305642 1112 8 0.36210012610583675 1112 9 -0.029988504891782952 1112 10-14 -0.016448051032647015 1112 15-19 -0.13456255185764832 1112 20-24 0.17420639877329336 1112 25-29 0.006261788722518702 1112 30-34 -0.03342116203846501 1112 35-39 -0.3091278214949753 1112 40-44 -0.26731395900662136 1112 45-49 -0.06209013417941378 1112 50-54 -0.5249852353389102 1112 55-59 -0.4453106137015297 1112 60-64 -0.31130269891812645 1112 65-69 -0.07606308113116711 1112 70-74 0.1993701227718887 1112 75-79 -0.595416240055421 1112 80-84 -0.24319117890239994 1112 85-89 0.0061571148904846495 1112 90-94 -0.05248271046230002 1112 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-0.788060954258448 2110 15-19 -0.5593268198477261 2110 20-24 -0.8780516657428379 2110 25-29 -0.8033163750491852 2110 30-34 -0.5297983332543481 2110 35-39 -0.5764231068547829 2110 40-44 -0.4971402617361562 2110 45-49 -0.8121645758667597 2110 50-54 -0.9363003718153351 2110 55-59 -0.44799051444594795 2110 60-64 -0.5562158502828822 2110 65-69 -0.45015737393513433 2110 70-74 -0.5620194553918836 2110 75-79 -0.41417554526634603 2110 80-84 -0.2220001119048831 2110 85-89 -1.0861009496256457 2110 90-94 -1.1647904027699951 2110 95-99 -0.9329128264827631 2110 100-104 -0.9061097843371044 2110 105-109 -0.5091027632015468 2110 110-114 -0.5377065525339901 2110 115-119 -0.39753226284425836 2110 120-124 -0.5219969830467335 2110 125-129 -0.6495910541892869 2110 130-134 -0.4447789140901932 2110 135-139 -0.03118777235949466 2110 140-144 -0.2669695379406818 2110 145-149 -0.6253256575112545 2110 150-154 -0.23413225796000603 2110 155-159 -1.0591297623675118 2110 160-164 -0.7176383352131879 2110 165-169 -0.9003800104766029 2110 170-174 -0.5481663303979261 2110 175-179 -0.14150350118346466 2110 180-184 0.06982279315979412 2110 185-189 -0.019399001329126975 2110 190-194 0.35540696089728385 2110 195-199 -0.46159972192156573 2110 200-204 -0.1513880049669254 2110 205-209 -0.5098444497875008 2110 210-214 0.04343184029483993 2110 215-219 0.4116312975117289 2110 220-224 -0.46306263242061263 2110 225-229 -0.39851940223405435 2110 230-234 0.6493676279626932 2110 235-239 -0.2448172041026737 2110 240-244 0.6558604342290231 2110 245-249 -1.4780278337965633 2110 250-251 -1.2720012311405746 2109 1 -0.24719561728315753 2109 2 0.29349832421141286 2109 3 -0.6201235254794071 2109 4 -0.8792971256036282 2109 5 -0.015166329909220622 2109 6 -0.9878073537362155 2109 7 -0.5538928545869766 2109 8 0.049714206940251415 2109 9 -0.4198190133663573 2109 10-14 -0.6245836442529864 2109 15-19 -0.5391518608537353 2109 20-24 -0.48816648258264195 2109 25-29 -0.6036990978485335 2109 30-34 -0.6122477591701525 2109 35-39 -0.23022299750540753 2109 40-44 -0.6994912732178307 2109 45-49 -0.5876703167087456 2109 50-54 -0.6606306069164916 2109 55-59 -0.6712819305203013 2109 60-64 -0.7412896611084605 2109 65-69 -0.7843290524479869 2109 70-74 -0.21778763472486418 2109 75-79 -0.8721307120241306 2109 80-84 -0.8901794448737235 2109 85-89 -1.0250293257874787 2109 90-94 -1.1088581993801654 2109 95-99 -1.53608395824876 2109 100-104 -0.5125067225547113 2109 105-109 -0.7174679901014827 2109 110-114 -0.5076354754426831 2109 115-119 -0.8346016996949999 2109 120-124 -0.7549658184759238 2109 125-129 -0.3074915462614527 2109 130-134 -0.673701822783471 2109 135-139 -0.8260483519111688 2109 140-144 -1.0955643985202457 2109 145-149 -0.8904978827709655 2109 150-154 -0.3254389829463378 2109 155-159 -0.5643785321870922 2109 160-164 -0.41681821492887394 2109 165-169 0.1376188960296787 2109 170-174 -0.206066822470099 2109 175-179 -0.0802131786028184 2109 180-184 -0.12126523308404558 2109 185-189 -0.39698238022470633 2109 190-194 -0.1992130500376561 2109 195-199 -0.22649857375316174 2109 200-204 -0.6854503341085341 2109 205-209 -0.17097074831127657 2109 210-214 -0.32862939535305813 2109 215-219 -0.7967339293882176 2109 220-224 -0.6027017794955185 2109 225-229 -1.6009250884013575 2109 230-234 -0.05582646717125783 2109 235-239 -0.6258450881923423 2109 240-244 -0.056003972550637826 2109 245-249 -0.20755216403166443 2109 250-251 1.6607489055461429 2108 1 0.8561135029751128 2108 2 0.3508026018546744 2108 3 0.5501751024463424 2108 4 0.4871274103124321 2108 5 0.5481912164991734 2108 6 0.8601883417171123 2108 7 1.1585096205273615 2108 8 1.068815632821341 2108 9 0.6525511776479007 2108 10-14 0.11752020293560861 2108 15-19 0.09786724057214258 2108 20-24 0.20341274797964104 2108 25-29 0.347067649528384 2108 30-34 0.1393260907087921 2108 35-39 -0.09613636850352947 2108 40-44 -1.1005180271439485E-4 2108 45-49 0.5696070575177714 2108 50-54 0.3977498073961314 2108 55-59 0.3211851127941969 2108 60-64 -0.018018205633623552 2108 65-69 -0.10033658540466917 2108 70-74 -0.011277007875243328 2108 75-79 0.4017998771606983 2108 80-84 0.09028867457745093 2108 85-89 0.554093622826997 2108 90-94 -0.02352056150014903 2108 95-99 0.3865956220579321 2108 100-104 0.2323950799742036 2108 105-109 0.0204927309100853 2108 110-114 0.004228931336974995 2108 115-119 -0.15738889366863162 2108 120-124 0.27242185975921274 2108 125-129 -0.16484963073818903 2108 130-134 0.05737162354420633 2108 135-139 0.08043537152170899 2108 140-144 -0.10142934336823828 2108 145-149 0.599251915453408 2108 150-154 0.1742650794695919 2108 155-159 0.09330239329044332 2108 160-164 -0.3258308595347401 2108 165-169 0.4158002250584758 2108 170-174 0.793717950196033 2108 175-179 1.1070884086987718 2108 180-184 0.5009031823047039 2108 185-189 0.7841852280615527 2108 190-194 1.2851560648399314 2108 195-199 1.8702999196554941 2108 200-204 0.5486631983100807 2108 205-209 1.1748457703865895 2108 210-214 1.382858363592323 2108 215-219 0.8745601249566874 2108 220-224 2.5276183711636087 2108 225-229 1.471014647945161 2108 230-234 2.489613403692342 2108 235-239 1.0868264210893344 2108 240-244 2.2812572596258462 2108 245-249 0.8788078036842357 2108 250-251 0.8045476679889703 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 57.0 16 365.0 17 192.0 18 91.0 19 56.0 20 47.0 21 39.0 22 53.0 23 78.0 24 78.0 25 120.0 26 150.0 27 202.0 28 306.0 29 373.0 30 504.0 31 552.0 32 650.0 33 799.0 34 1121.0 35 1548.0 36 2548.0 37 5268.0 38 2268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.078156312625254 25.12453478385342 7.311766389922703 28.485542513598627 2 50.48955052963069 21.162324649298597 18.368164901231033 9.97995991983968 3 47.220154594904095 19.639278557114228 20.13741769252791 13.003149155453764 4 48.462639564843975 17.81849413111938 17.17721156598912 16.541654738047523 5 35.356427139994274 17.371886630403665 33.518465502433436 13.753220727168621 6 35.356427139994274 17.062696822215862 33.20354995705697 14.377326080732894 7 17.990266246779274 51.38276553106213 17.520755797308905 13.1062124248497 8 30.415115946178073 21.179501860864587 33.884912682507874 14.520469510449471 9 16.4214142570856 18.82622387632408 33.64443172058402 31.107930146006296 10-14 29.21614657887203 36.419123962210136 20.26910964786716 14.095619811050671 15-19 25.922702547953048 34.06012024048096 18.786143716003437 21.231033495562553 20-24 28.461494417406243 30.8903521328371 24.125966218150587 16.52218723160607 25-29 17.939879759519037 31.110220440881765 34.5674205553965 16.382479244202692 30-34 21.101631835098768 29.192098482679647 29.533352419123965 20.172917263097624 35-39 22.61666189521901 29.921557400515315 27.080446607500715 20.38133409676496 40-44 20.041225307758374 31.23275121671915 26.30518179215574 22.42084168336673 45-49 21.698253650157458 29.67191525908961 25.465788720297738 23.164042370455196 50-54 24.056112224448896 30.39793873461208 24.196965359290008 21.34898368164901 55-59 24.380188949327223 25.34783853421128 28.57142857142857 21.700543945032923 60-64 23.925565416547382 25.38333810478099 28.01717721156599 22.673919267105642 65-69 18.654451760664188 27.066704838247922 29.628399656455766 24.65044374463212 70-74 19.655310621242485 26.45061551674778 31.015173203549956 22.878900658459777 75-79 20.106498711709133 26.893787575150302 28.582880045805897 24.41683366733467 80-84 22.51588892069854 30.519324363011734 23.825937589464644 23.13884912682508 85-89 20.578299456054967 27.51674778127684 25.342112797022615 26.56283996564558 90-94 22.460921843687377 31.436587460635558 26.268537074148295 19.83395362152877 95-99 24.160320641282564 27.94846836530203 28.154594904093905 19.7366160893215 100-104 23.128542799885484 27.756083595762952 25.755511022044086 23.35986258230747 105-109 25.746349842542227 28.718007443458344 23.598053249355853 21.937589464643573 110-114 22.566275407958774 26.155167477812768 31.07586601774979 20.202691096478674 115-119 25.086744918408243 27.20526767821357 27.503006012024052 20.20498139135414 120-124 20.52791296879473 27.52018322359004 25.45777268823361 26.49413111938162 125-129 19.259089607787004 25.011165187517893 29.889493272258804 25.840251932436303 130-134 24.235900372172917 28.80274835385056 27.243057543658743 19.71829373031778 135-139 24.11795018608646 25.36501574577727 24.77182937303178 25.745204695104494 140-144 22.026910964786715 27.405668479816775 24.59662181505869 25.97079874033782 145-149 23.70684225594045 25.598625823074723 28.81419982822788 21.880332092756944 150-154 22.57200114514744 29.614657887202977 23.443458345261952 24.36988262238763 155-159 21.4715144574864 26.155167477812768 30.969367306040652 21.40395075866018 160-164 23.335814486115087 32.618379616375606 24.01488691669052 20.03091898081878 165-169 23.435442313197825 29.218436873747493 25.539078156312627 21.807042656742055 170-174 22.562839965645576 31.33008874892643 24.68021757801317 21.426853707414832 175-179 20.265674205553967 29.26538791869453 26.773547094188377 23.695390781563127 180-184 22.751789292871457 28.283996564557683 27.070140280561123 21.894073862009733 185-189 22.25594045233324 26.4723733180647 28.13970798740338 23.131978242198684 190-194 20.97108502719725 27.725164614944177 27.785857429144002 23.517892928714573 195-199 21.368451188090468 32.95390781563126 25.329516175207555 20.348124821070712 200-204 23.96564557686802 27.80303464070999 24.87832808474091 23.352991697681077 205-209 22.60864586315488 26.908674491840824 28.831377039793875 21.65130260521042 210-214 26.85485256226739 26.357858574291438 28.573718866304038 18.21356999713713 215-219 24.019467506441455 29.711995419410247 27.213283710277697 19.055253363870598 220-224 22.137990266246778 28.622960206126542 26.536501574577727 22.702547953048953 225-229 27.393071858001715 27.790438018894932 25.766962496421414 19.049527626681936 230-234 25.43143429716576 28.957343257944462 25.931863727454914 19.67935871743487 235-239 24.947036931004867 27.554537646722014 25.446321213856283 22.052104208416836 240-244 26.49642141425709 32.857715430861724 23.176638992270256 17.469224162610935 245-249 27.11709132550816 29.25966218150587 23.971371314056686 19.651875178929288 250-251 25.728600057257374 27.503578585742915 20.234755224735185 26.533066132264526 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.3333333333333333 25 0.6666666666666666 26 0.3333333333333333 27 0.0 28 3.0 29 3.3333333333333335 30 8.999999999999998 31 7.9999999999999964 32 6.666666666666664 33 12.333333333333337 34 36.66666666666665 35 277.66666666666833 36 281.0000000000015 37 76.99999999999999 38 132.9999999999994 39 215.00000000000173 40 402.99999999999454 41 347.33333333333104 42 559.9999999999937 43 640.3333333333362 44 730.6666666666798 45 718.0000000000117 46 775.3333333333516 47 636.6666666666691 48 742.6666666666812 49 941.6666666667038 50 675.6666666666736 51 257.66666666666947 52 93.66666666666642 53 53.66666666666677 54 33.66666666666663 55 23.999999999999982 56 19.999999999999996 57 12.666666666666671 58 7.33333333333333 59 7.666666666666663 60 9.666666666666666 61 4.333333333333333 62 2.6666666666666665 63 1.9999999999999998 64 1.6666666666666665 65 1.0 66 0.0 67 0.3333333333333333 68 0.3333333333333333 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.3333333333333333 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.3333333333333333 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-154 0.0 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 0.0 180-184 0.0 185-189 0.0 190-194 0.0 195-199 0.0 200-204 0.0 205-209 0.0 210-214 0.0 215-219 0.0 220-224 0.0 225-229 0.0 230-234 0.0 235-239 0.0 240-244 0.0 245-249 0.0 250-251 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 17465.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.27072684951399 #Duplication Level Percentage of deduplicated Percentage of total 1 75.28585656359125 37.09388874376395 2 15.705491317470308 15.476419454809864 3 4.9991066263397155 7.389288512339387 4 1.868385439060141 3.6822683447016593 5 0.8590016590839815 2.11618180540031 6 0.4979301872263921 1.4720029346975356 7 0.19916942979212232 0.6869255800442778 8 0.08713644245342243 0.3434620683808777 9 0.03734417721319331 0.1655977279401983 >10 0.2116169918845655 1.747975912425253 >50 0.07468834976546751 2.9316929265077984 >100 0.13692864123669043 14.958993823750044 >500 0.037344174882733755 11.935302165238863 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 725 4.151159461780704 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 651 3.7274549098196395 No Hit CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC 570 3.263670197537933 No Hit GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 486 2.7827082736902375 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC 375 2.14715144574864 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 274 1.5688519896936732 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG 235 1.3455482393358145 No Hit GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 203 1.1623246492985972 No Hit GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 174 0.996278270827369 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA 172 0.984826796450043 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT 150 0.8588605782994561 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA 129 0.7386200973375322 No Hit CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG 122 0.6985399370168909 No Hit GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA 119 0.6813627254509018 No Hit GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC 98 0.561122244488978 No Hit GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA 95 0.5439450329229888 No Hit GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 93 0.5324935585456628 No Hit GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA 65 0.37217291726309765 No Hit GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA 64 0.36644718007443455 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 63 0.36072144288577157 No Hit GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 33 0.18894932722588034 No Hit GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA 30 0.17177211565989123 No Hit GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 28 0.16032064128256512 No Hit GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT 24 0.13741769252791297 No Hit GGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGT 23 0.13169195533924993 No Hit GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA 22 0.1259662181505869 No Hit GGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTC 19 0.10878900658459778 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10-14 0.010306326939593474 0.0 0.0 15-19 0.01832235900372173 0.0 0.0 20-24 0.022902948754652163 0.0 0.0 25-29 0.022902948754652163 0.0 0.0 30-34 0.0286286859433152 0.0 0.0 35-39 0.03320927569424563 0.0 0.0 40-44 0.04008016032064128 0.0 0.0 45-49 0.056112224448897796 0.0 0.0 50-54 0.07328943601488691 0.0 0.0 55-59 0.0858860578299456 0.0 0.0 60-64 0.12024048096192383 0.0 0.0 65-69 0.18551388491268253 0.0 0.0 70-74 0.2805611222444889 0.0 0.0 75-79 0.37560835957629546 0.0 0.0 80-84 0.4580589750930432 0.0 0.0 85-89 0.625250501002004 0.0 0.0 90-94 0.9069567706842256 0.0 0.0 95-99 1.1199541941024909 0.0 0.0 100-104 1.3604351560263384 0.0 0.0 105-109 1.6959633552819926 0.0 0.0 110-114 2.170054394503292 0.0 0.0 115-119 2.623532779845405 0.0 0.0 120-124 3.129687947323218 0.0 0.0 125-129 3.6873747494989977 0.0 0.0 130-134 4.237045519610651 0.0 0.0 135-139 4.84740910392213 0.0 0.0 140-144 5.470369310048669 0.0 0.0 145-149 6.139135413684512 0.0 0.0 150-154 6.945319209848267 0.0 0.0 155-159 7.702261666189523 0.0 0.0 160-164 8.570283423990839 0.0 0.0 165-169 9.386773547094188 0.0 0.0 170-174 10.50214715144575 0.0 0.0 175-179 11.469796736329801 0.0 0.0 180-184 12.393930718580016 0.0 0.0 185-189 13.398225021471514 0.0 0.0 190-194 14.384196965359289 0.0 0.0 195-199 15.503006012024048 0.0 0.0 200-204 16.56455768680218 0.0 0.0 205-209 17.600916117950185 0.0 0.0 210-214 18.61208130546808 0.0 0.0 215-219 19.5751503006012 0.0 0.0 220-224 20.5302032636702 0.0 0.0 225-229 21.41311193816204 0.0 0.0 230-234 22.312052676782137 0.0 0.0 235-239 23.320927569424562 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 320 0.0 245.00002 1 TCGGGAA 10 0.0014638032 245.00002 3 CGAGAGG 60 0.0 245.0 245 GAGGGGC 30 1.6370905E-11 245.0 9 ATTAGTA 315 0.0 244.99998 7 TATTAGT 315 0.0 244.99998 6 GGGTTAT 325 0.0 241.23074 2 GGTTATT 325 0.0 241.23074 3 GTTATTA 325 0.0 241.23074 4 TTATTAG 330 0.0 237.57576 5 GGAGCGA 235 0.0 229.36171 5 GCGAAAG 235 0.0 229.36171 8 GAGCGAA 235 0.0 229.36171 6 TTAGTAG 340 0.0 226.98529 8 CGAAAGC 240 0.0 224.58333 9 AGCGAAA 240 0.0 224.58333 7 TAGTAGA 345 0.0 223.69565 9 GAGAGCG 30 4.605681E-9 204.16667 7 GGGGGGA 340 0.0 194.55882 1 GGGAGCG 280 0.0 192.5 4 >>END_MODULE