FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated h3.3420000000856c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated h3.3420000000856c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259602
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG56152.162926325683161No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA46361.7858105869754468No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC39931.5381237432685417No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG35291.359388602553139No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG28881.1124721689355244No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG22590.8701781958536529No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC19900.7665580388440767No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC17390.6698715726381153No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16540.6371291438432678No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA14330.5519988289766643No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA13460.5184859900925263No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13200.5084706589317494No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA12080.46532769393147977No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11850.45646797790463867No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA10940.42141431884191954No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG10230.3940647606721057No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9940.38289381437739306No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC9840.3790417639309404No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC9550.36787081763622775No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT9210.35477384611828877No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT7690.2962226793322085No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA7450.2869777582607222No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA6650.256161354689101No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6140.2365158974121925No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT5860.2257301561621251No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG5230.20146223834947344No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5190.19992141817089235No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4870.1875948567422439No Hit
GGGGGGAGCGAAAGCAGGGCCTGACAGCAGAGACAGCCCAGCCAGGAGCA4370.16833460450998067No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT3830.14753353209913636No Hit
GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC3720.14329627660803845No Hit
GGGGGGAGCGAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCA3570.13751820093835948No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3510.1352069706704879No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC3510.1352069706704879No Hit
CGGGTTATTAGTAGAACAAGAGATAAGAGCTTTCTCGTTTCAGCTTATTT3510.1352069706704879No Hit
GGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAA3420.13174012526868054No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT3380.13019930509009944No Hit
GGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCA3350.12904368995616367No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT3020.11633192348286994No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT3000.11556151339357941No Hit
CGGGTTATTAGTAGAAACAAGGTTCTATCAAATTACAGTCCCACCAATGA2840.10939823267925516No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA2810.10824261754531937No Hit
GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC2630.10130892674170462No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA34500.0240.739121
TTATTAG34750.0237.597145
GGGTTAT34800.0237.255752
GGTTATT35450.0232.90553
TATTAGT35700.0231.960786
GTTATTA35850.0229.965134
ATTAGTA36450.0227.524027
TAGTAGA38100.0219.278239
TTAGTAG39000.0212.647458
GGGGGGA73150.0208.827741
GGAGCGA48550.0201.853765
GAGCGAA49250.0199.23356
AGCGAAA49400.0198.380557
GGGAGCG49900.0196.147314
GCGAAAG50250.0195.024878
CGAAAGC51550.0190.81969
GGGGAGC79550.0189.717163
GGGGGAG84250.0179.569732
GAGCAAA32750.0163.458026
GCAAAAG33450.0158.57258
Produced by FastQC (version 0.11.2)