FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated h2.342000000084e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated h2.342000000084e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548653
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG34840.635009742040962No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC24650.44928215101348207No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG24300.4429028912627836No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT20380.37145518205496003No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA18650.3399234124300788No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA17520.3193275166635378No Hit
CGGGTTATTAGTAGAAACAAGGACAAGTGCAGTCAGGACAATGAGATAAA17000.3098497593196428No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG14660.2671998512721155No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC14020.25553491915655246No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG12850.23420996513278886No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG12790.23311637774695482No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA12710.23165826123250943No Hit
GGGGGGAGCGAAAGCAGGAGAAGAAGCTCTCACCACTATTCTAGCTCTTA11600.2114268945945798No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC11470.20905745525860606No Hit
CGGGTTATTAGTAGAAACAAGGAGACACTAGGGTATTTCTCCTTTTGCCT10600.19320043816401258No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT8250.1503682655521796No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTT8140.1483633553448172No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATT7880.14362447667286973No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA7590.13833880430800524No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCCGC7500.13669842322925418No Hit
CGGGTTATTAGTAGAAACAAGGACAGAAAGGTCAGCAGTTCTTGAGAACA7340.13378219020036344No Hit
CGGGTTATTAGTAGAAACAAGGGGGCTGCACAGTCCCTGGAGAGCCTGTG7310.13323539650744642No Hit
GGGGGGAGCGAAAGCAGGAAGCCAGTACCAGGGCTAAGCTGGAAAGGCAA7290.13287086737883508No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG7030.1281319887068876No Hit
GGGGGGAGCGAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA6940.12649160762813655No Hit
GGGGGGAGCAAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG6670.1215704643918834No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG6230.11355082356243382No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC5990.10917647401909768No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACA5920.10790062206895797No Hit
CGGGTTATTAGTAGAAACAAGGTCAAAAGACGGTGTGTGTGTCCGTGTTT5880.10717156381173527No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC5870.10698929924742961No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG5490.10006324580381407No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA56300.0230.636172
ATCCGGA19950.0229.645872
GATCCGG20500.0227.069981
TCGGGAG47650.0226.743913
GATCGGG60400.0220.861941
CGGGTTA32950.0220.459721
GGGTTAT33200.0218.061662
CGGGAGA53400.0217.468844
TCCGGAG17250.0216.591143
TTATTAG33750.0216.322865
GTTATTA33800.0214.915594
TATTAGT34700.0211.106526
CCGGAGA19300.0208.18364
ATTAGTA35700.0207.595127
GGTTATT35350.0205.14563
GGAGCGA87150.0204.093495
TAGTAGA36450.0203.995769
CGAAAGC89200.0203.934899
GCGAAAG88850.0203.221688
GAGCGGC21600.0203.029549
Produced by FastQC (version 0.11.2)