FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated h1.3420000000846d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated h1.3420000000846d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318859
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC13530.42432548555944793No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT10750.33713961343415116No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG9420.2954283868418329No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG9060.284138130019852No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC8850.27755214687369656No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA8400.26343932584622043No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC7910.24807203183852422No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG7140.22392342696928735No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA6630.20792889647148113No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC6210.19475693017917012No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG5790.18158496388685907No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5460.1712355618000433No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA5080.15931806848795235No Hit
GGGGGGAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG4600.1442643927253112No Hit
GGGGGGAGCGAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA4570.1433235379901461No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG3710.11635236891541403No Hit
GGGGGGAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA3650.11447065944508387No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT3480.10913914927914846No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG3410.1069438215637633No Hit
GGGGGGAGCGAAAGCAGGGTGAGTGAGGGAGATGCGTGGGTTGGGTAACA3370.10568934858354319No Hit
GGGGGGAGCGAAAGCAGGCTGGAAACTTCAGAGCAGTTGCTCGGATTACC3340.10474849384837812No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA3240.10161231139782786No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT17400.0216.135062
ATCGGGA17000.0214.73532
ACGGGAG13000.0212.961553
TATTAGT17500.0212.099996
CGGGAGA29900.0211.814394
CGGGTTA17800.0211.278091
AACGGGA14900.0208.003372
TTATTAG17950.0207.46525
TCGGGAG17450.0205.687673
ATTAGTA18350.0204.277947
TTAGTAG18400.0203.057058
GTTATTA18400.0203.057054
GAGCGGC13850.0200.776179
TAGTAGA18600.0200.215049
GGTTATT18850.0199.509283
GAGGGGC40550.0199.383479
GATCGGG19000.0194.06581
AGAGCGG16150.0193.421058
GGAGCGA34900.0190.594545
GGGAGCG35200.0188.622164
Produced by FastQC (version 0.11.2)