FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated g7.3420000000875e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated g7.3420000000875e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGGGAAAGCGTCGGGTTGGGGAAAAGGGGAAGGCCTGGTGGTGAATT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA11.3513513513513513No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGAGGGGCTAGGGTAGAAAT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGCCCGTGAGTCAGTAGTGAAACCAGAAGT11.3513513513513513No Hit
GATCTGGAGAGCGGCGGGTAGGGGAAGAGTGTAAGGTATAGGGGAAAGAT11.3513513513513513No Hit
CATTGCCATCATCCACTTCATTCTGAGTGCGGGGTTCTTCTCTTGCCTTC11.3513513513513513No Hit
CGGTTTATTAGTAGAAACAAGGGCTTGAAGGGAACAACTGTTTATTTTTT11.3513513513513513No Hit
TGGCTGGATCCTGGGAAATCCAGAGTGTGAATCACTCTCCACAGCAAGCT11.3513513513513513No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC11.3513513513513513No Hit
AGGGGGGGGCATCAGCGGAGTCCTTGAATGTAATCTTGACACGGTTCGAG11.3513513513513513No Hit
GAACGGTAGAGCGGCGGGTAGGGGAAGAGGGTAACGCCCGGGGGAGGAAC11.3513513513513513No Hit
GATTGGAAGAGGGGCGGGGTGGGGAAAAGGGGAAGGCCTAGGGGAGATAT11.3513513513513513No Hit
GGGAGCAACAGCAGGGGGAGTGGGAGAAGGAGAAGCAGGCTTCCGGCCTA11.3513513513513513No Hit
GACCAGGAAAAGCGCGCTCCGGGGAAAAAGGCATGCTTCTTAGTAAATTT11.3513513513513513No Hit
ATCTCTTCTGAGTTTCAGGGCTTCTAACTGCATATTGGACATCTTTACCT11.3513513513513513No Hit
GAATGGGAGAGGGGCGTGGTGGGGAAAAGGGGAAGGCCATGGGGTGAATT11.3513513513513513No Hit
CATTCCACGATTTGCTTTCCAACCAGAGCGGATTGTTTGCTATTCTCGCC11.3513513513513513No Hit
GATCGGAAGAACCGGGAGCGGGGAAAAGTGCAGATGCAGCGGGTTGAGGC11.3513513513513513No Hit
ACAAATATCATTTATGCAAGCGTTACAACTACTGCTTGAAGTAGAACAAG11.3513513513513513No Hit
GACACGGAATAGGGCGGGGTGGGGGAAGGGGTAGGCAATAATGGGGAAAT11.3513513513513513No Hit
GATCGGAAAAAGGGCGGGGAGGGGAAGAGGGGAAGGCCTAGAGTAGAATT11.3513513513513513No Hit
AATGCAAATCTGAATCCTTGAAACCACCTTCTTCATCAGTTCATATTTCA11.3513513513513513No Hit
GATTTGGAGAAGGGCGGGTTGGGGAAAAGGGGAAGGCCTAGGGTAAATAC11.3513513513513513No Hit
ACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGC11.3513513513513513No Hit
GATTGGAAGAGGGGCGGGGAGGGGAAGAGGGGAGGGTATAGGGGAGAAAT11.3513513513513513No Hit
GGGAGCAAAAGCGGGCAGATCAGTTAAATGGATGTCAATCCGACTCTACT11.3513513513513513No Hit
GATCGGGAGCGTGGCGTGATTGGGAAAAGGGTAATCCCTCGGGTGAATTC11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGGTGACCAAACCATAATGGACCCAAACACCATG11.3513513513513513No Hit
CCAAGCCTGACGGCTAGGAAGACGCCAGCCCCACGCGTATTAATATGACA11.3513513513513513No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGGCCTGGTGTAGATCT11.3513513513513513Illumina Single End PCR Primer 1 (96% over 33bp)
GGTATGGAAAGGGGCGGGTAGGGGAAGAGTGGACGGCCTGGTGTTAATTT11.3513513513513513No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGGGCCTAGTGTAGAGTT11.3513513513513513No Hit
GTATGGAAGAGAGTCGGGGAGGGGAAATGGGTTGCAGTTCAGGTAGATCT11.3513513513513513No Hit
GGTCGGGAAAGCGGCGTGGTGGGGAAAAATGGGTCTTCTAGCGGAGGTCT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCAGGCTTCTGAAATCCTA11.3513513513513513No Hit
GATCGGAAGAGCGGCGGGGAGGGGAAGAGGGTGGGGCGCTGTGGAGATCT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA11.3513513513513513No Hit
AGGGTTATTAGTAGAAACAAGGGAGTTTTTTACTCTATCTCTATGTTGAC11.3513513513513513No Hit
ATGACCTGCGTAAAAAGTACAAGGCATACCCGGAACCCGTGTGAGAGCAT11.3513513513513513No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG11.3513513513513513No Hit
GAACGGGAAAATGTCGTGTAGGGGAAAAGCGTAAGCATGAGGGAATATCG11.3513513513513513No Hit
GATTGGGAGGAGGGCGGGGTGGGGAAAAGGGGAGGGCCTAGGGGAAGAAT11.3513513513513513No Hit
GATCCGGAGAGCGGCGCGTAGGGGAAGAGGGGATGGCCTTGGGTAAATAT11.3513513513513513No Hit
GACCTCCCCTTTCCGAGAGCCCCTCCGCCTCCACAGATATCTGTGACATG11.3513513513513513No Hit
GATCGGAAAAGCGGCGGGGAGGGGAAAAGGGTAGGGGCTAGGGGAGAACT11.3513513513513513No Hit
TGGAATGGATCTTGAAAGAGGAATCCAGCGAGACACTTAGAATGACAATT11.3513513513513513No Hit
ATAGTTTAACTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGG11.3513513513513513No Hit
GGGGTGAGCGATACCAGGCAGAGCGGCAGGCAGAGGCACCGGGAGAAGCA11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA11.3513513513513513No Hit
GTCCATTACTTGGTCCATCGGTCATTACAGTAAAGCAAGAACCATTTACA11.3513513513513513No Hit
GAACGGGAGAGCGGCGTGGAGGGGAAAAGGGGAGGGCATAGTGGAGAGCA11.3513513513513513No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC11.3513513513513513No Hit
GAAGAAATGCGGCACAGATTGAAAGCGACAGAGAATAGTTTCGAACAAAT11.3513513513513513No Hit
ATTGACTGATTCAAGCTGGAAAGAACTTGATGAAATAGGAGAAGATGTTG11.3513513513513513No Hit
GATTTGGAATCTGCCTTTTAAGGGAATTGGGTCTCGGCTCGGCGTATCAA11.3513513513513513No Hit
GAACGGAAAAGGGTCGGGGAAGGGAAAAAGGTTGGCCTTAAAGGAAGATC11.3513513513513513No Hit
AGTGAGATCTGTGTGTAGTTTATACTGTTTTTACATCAAATGAAGTTGAT11.3513513513513513No Hit
CAAAGTCATGCCCACTTGCTACTGCAAGCCCATACACACAAGCAGGCAGG11.3513513513513513No Hit
CAACACAGGAGAAATAGGTGTTATTAAGGATGAATAGACGAATCATAAGC11.3513513513513513No Hit
AAGCCGTTAGAAGTGCCATTGTCTTTATTCGGTTCTCGATTACATTGTCT11.3513513513513513No Hit
GATCGGGAGAGGGGCGGGTAGGGGAAGAGGGGAAGGCCTAGGGGAGAACT11.3513513513513513No Hit
CTCTTTGCGTGCGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTG11.3513513513513513No Hit
GGATCCAGTTACGGTTTCTTGGGGGAAAGGGGGAGCTCCTGTATAAGGCA11.3513513513513513No Hit
ATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATT11.3513513513513513No Hit
TATTCCATAATCTCATATACCTTTTCCTAGTGAACCTTCTCTCCCATCAT11.3513513513513513No Hit
GGAGGAAGCAAAATTAAACAGAGAAGAAATGGATGGGGTAAAGCTGGAAT11.3513513513513513No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11.3513513513513513No Hit
TTTTGCTACATGCGAGGAGGCTATTGAGCGAATTGGGCGCGGTTACGAAA11.3513513513513513No Hit
GATCGGGAGAGAGGCGGGTAGGGGAAAAGGGGAGGTCCCGGGGGTGAATT11.3513513513513513No Hit
GTGAAGGAAAGGGGCGGGGAGGGGGAGAGTGGCAAACCTGGTGGAGAATT11.3513513513513513No Hit
GCTAGGGAAACCGGCGTGGTGGGGAACAGCGTGAGGCCCGGGGAACAAGT11.3513513513513513No Hit
AAAATTTCAAACAGCTGGCCAGGGGACAATGATGGGTAAAGTAAGAGAAA11.3513513513513513No Hit
AGCTCCAGTGCTGGCCTGAAAGATGACCTTCTTGTAAATTTGCAGGCCGA11.3513513513513513No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAG50.0245.000026
GAGAAGG50.0245.000027
CACACCC50.0245.00002245
AGAAGGG50.0245.000028
TGGAGAA50.0245.000025
GATTTGG50.0245.000021
ATTTGGA50.0245.000022
TTTGGAG50.0245.000023
TTGGAGA50.0245.000024
GAAGGGC50.0245.000029
AAAAAAA550.022.27272875-79