FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated g4.342000000085d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated g4.342000000085d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129489
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA57124.411185506104766No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG48233.7246407030713033No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC40293.1114612051988972No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG30082.3229772413100727No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG26452.0426445489578264No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC25171.94379445358293No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT23021.7777571840079083No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG19961.5414436747522955No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT15551.2008742055309718No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA14971.1560827560642215No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10940.8448594089073204No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10910.8425426097969712No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA10200.7877116975187082No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA8870.6850002702932295No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6580.5081512715365784No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG5880.4540926256284317No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5130.3961726478697032No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4500.34751986655237127No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4380.3382526701109747No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4050.3127678798971341No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA2800.21623458363258652No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA2370.18302712971758217No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT2030.15677007313362523No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA1670.12896848380943557No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGG201.5564365E-7244.99998245
TTCGGAA100.0014746934244.999983
CGGGTTA21950.0243.325741
GGGTTAT22000.0242.21592
GGTTATT22000.0241.659073
TATTAGT22250.0240.59556
TTATTAG22150.0240.022575
ATTAGTA22350.0239.519037
GTTATTA22550.0235.764954
GGGGGGA24800.0233.145161
TAGTAGA23800.0228.52949
GCGAAAG18650.0220.040228
GAGCGAA18700.0219.451876
AGCGAAA18600.0219.314517
GGAGCGA18800.0218.284585
CGAAAGC18750.0218.213339
GGGGGAG27700.0208.294222
TTAGTAG26150.0205.65018
GGGGAGC28950.0198.877383
GGTTAGT254.7337926E-7196.03
Produced by FastQC (version 0.11.2)