FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated g3.34200000008550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated g3.34200000008550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293516
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA58081.9787677673448807No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG39621.349841235230788No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC37441.2755693045694272No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25700.8755911091729243No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG19890.6776461930525083No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC17670.6020114746725902No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG16350.5570394799602066No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC15580.5308058163779829No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA15240.5192221207702477No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG13920.474250126057864No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT13310.45346761334986846No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA12030.40985840635604187No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG11640.3965712261001104No Hit
CGGGTTATTAGTAGAAACAAGGTTCTATCAAATTACAGTCCCACCAATGA10400.35432480682484085No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC10380.35364341296556234No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA9820.33456438490576323No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC9370.3192330230719961No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC8920.3039016612382289No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG8740.29776911650472204No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA8700.29640632878616496No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT8110.27630520993744806No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA7430.2531378187219777No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7380.25143433407378135No Hit
CGGGTTATTAGTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCC6840.23303669987326076No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6770.23065182136578583No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT6740.22962973057686803No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA6500.22145300426552558No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC6400.21804603496913288No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT6200.21123209637634743No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA6130.2088472178688726No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT5310.1809100696384524No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA5310.1809100696384524No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5210.1775031003420597No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA4980.16966707096035652No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG4740.16149034464901402No Hit
GGGGGGAGCGAAAGCAGGGCCTGACAGCAGAGACAGCCCAGCCAGGAGCA4620.15740198149334278No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC4590.15637989070442498No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA4590.15637989070442498No Hit
GGGGGGAGCGAAAGCAGGGACTTTTGGGCAGCATTAAGATGTATGAGGTG4450.15161013368947518No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT4380.1492252551820003No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4300.14649967974488615No Hit
GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA4110.14002643808174No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT3980.13559737799642949No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGGCAGATCAGGCCTAGGGGGAA3850.13116831791111896No Hit
GGGGGGAGCGAAAGCAGGAACAGCAGCACTGGGTTCCTCTGCTGTCACTT3790.12912413633328337No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGTATTTGTACTGTGACTGGCTCCC3690.12571716703689068No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT3560.12128810695158015No Hit
CGGGTTATTAGTAGAAACAAGGAAAAGAATATGAATGAATGATCAGCTCA3550.12094741002194088No Hit
CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA3500.11924392537374454No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGGCAGATCAGGCCTAGGGGGAA3200.10902301748456644No Hit
CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA3170.10800092669564862No Hit
CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC3170.10800092669564862No Hit
GGGGGGAGCGAAAGCAGGGATGTGCACAGATACTTGTATACCCATGTTCA3150.10731953283637007No Hit
GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT3120.10629744204745228No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA2990.10186838196214176No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC2980.1015276850325025No Hit
GGGGGGAGCAAAAGCAGGTTGCCAACTCCGCTTTTGTGGAGCGAGTGCGA2960.10084629117322395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA48500.0242.726821
GTTATTA50550.0233.367954
GGGTTAT50650.0233.149082
TTATTAG51000.0231.308825
GGTTATT51650.0228.872213
TATTAGT51950.0227.078936
ATTAGTA52850.0223.907297
TAGTAGA54250.0219.032249
GGGGGGA76750.0218.504871
CGCGAAA354.9112714E-11210.000027
TTAGTAG56750.0209.383278
GGAGCGA52350.0202.879655
AGCGAAA53650.0199.333637
GAGCGAA53350.0199.306476
GGGAGCG53250.0198.99064
GCGAAAG54850.0196.759348
CGAAAGC56050.0193.421079
GGGGAGC88500.0189.07913
GGGGGAG93300.0179.220262
GGAGCAA37550.0166.704395
Produced by FastQC (version 0.11.2)