FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated g2.342000000084d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated g2.342000000084d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378116
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA44441.1753007013720658No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC43571.1522918892614964No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG30860.8161516571634102No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG27230.7201493721503454No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG25240.6675200203112273No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA17590.4652011552010494No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC16550.437696368310254No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15110.3996128172306911No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA15100.399348348125972No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG14870.3932655587174306No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG14430.38162891810978644No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11600.3067841614742566No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA10700.28298194204952976No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10200.2697584868135704No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA9190.24304710723693257No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8540.22585661543018543No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC8210.2171291349744523No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT8070.21342656750838368No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC6890.18221921315151962No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6830.1806323985232045No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG6820.18036792941848534No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA6560.17349173269578647No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA5800.1533920807371283No Hit
GGGGGGAGCGAAAGCAGGGCAGAGCCAGCTCCAGTCACTGCTCTCTGGTG5640.1491605750616213No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5110.1351437125115044No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4990.13197008325487417No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4970.1314411450454358No Hit
GGGGGGAGCGAAAGCAGGTAAAGCATTAGTGCTGTTTTCCCAGAAGACCC4640.12271366458970263No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4280.11319277681981191No Hit
CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA3900.10314295084048282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT39100.0229.021742
TATTAGT39750.0228.050316
CGGGTTA39800.0224.993711
ATCCGGA5800.0223.879322
ATTAGTA40750.0223.656437
TTATTAG40100.0223.310475
TAGTAGA41700.0220.323759
GTTATTA40700.0220.319414
GGTTATT41750.0214.778443
TTAGTAG43600.0209.598638
GATCCGG6350.0208.346451
ATCGGGA17000.0200.323532
GGAGCGA60100.0199.546595
AGCGAAA60300.0198.884747
GAGCGAA60600.0197.698036
TCCGGAG6350.0196.771653
GCGAAAG61700.0196.754448
CGAAAGC63050.0196.233159
GGGGAGC85950.0193.406053
GGGAGCG61900.0192.556554
Produced by FastQC (version 0.11.2)