##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n02 flu mrt-pcr updated f7.34200000008741.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	76
Sequences flagged as poor quality	0
Sequence length	251
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.05263157894737	0.0	0.0	0.0	0.0	0.0
2	24.460526315789473	0.0	0.0	0.0	0.0	0.0
3	23.68421052631579	0.0	0.0	0.0	0.0	0.0
4	22.960526315789473	0.0	0.0	0.0	0.0	0.0
5	24.236842105263158	0.0	0.0	0.0	0.0	0.0
6	26.960526315789473	0.0	0.0	0.0	0.0	0.0
7	23.736842105263158	0.0	0.0	0.0	0.0	0.0
8	26.42105263157895	0.0	0.0	0.0	0.0	0.0
9	24.94736842105263	0.0	0.0	0.0	0.0	0.0
10-14	25.60263157894737	0.0	0.0	0.0	0.0	0.0
15-19	25.594736842105267	0.0	0.0	0.0	0.0	0.0
20-24	27.573684210526316	0.0	0.0	0.0	0.0	0.0
25-29	26.0	0.0	0.0	0.0	0.0	0.0
30-34	25.689473684210526	0.0	0.0	0.0	0.0	0.0
35-39	26.176315789473684	0.0	0.0	0.0	0.0	0.0
40-44	26.189473684210526	0.0	0.0	0.0	0.0	0.0
45-49	25.273684210526316	0.0	0.0	0.0	0.0	0.0
50-54	26.239473684210527	0.0	0.0	0.0	0.0	0.0
55-59	26.75	0.0	0.0	0.0	0.0	0.0
60-64	24.0	0.0	0.0	0.0	0.0	0.0
65-69	25.323684210526316	0.0	0.0	0.0	0.0	0.0
70-74	26.155263157894733	0.0	0.0	0.0	0.0	0.0
75-79	28.281578947368423	0.0	0.0	0.0	0.0	0.0
80-84	26.27894736842105	0.0	0.0	0.0	0.0	0.0
85-89	25.82894736842105	0.0	0.0	0.0	0.0	0.0
90-94	25.444736842105264	0.0	0.0	0.0	0.0	0.0
95-99	25.29736842105263	0.0	0.0	0.0	0.0	0.0
100-104	25.015789473684215	0.0	0.0	0.0	0.0	0.0
105-109	25.284210526315785	0.0	0.0	0.0	0.0	0.0
110-114	25.210526315789473	0.0	0.0	0.0	0.0	0.0
115-119	24.339473684210525	0.0	0.0	0.0	0.0	0.0
120-124	25.065789473684212	0.0	0.0	0.0	0.0	0.0
125-129	24.66842105263158	0.0	0.0	0.0	0.0	0.0
130-134	24.95263157894737	0.0	0.0	0.0	0.0	0.0
135-139	24.731578947368416	0.0	0.0	0.0	0.0	0.0
140-144	25.542105263157893	0.0	0.0	0.0	0.0	0.0
145-149	24.973684210526315	0.0	0.0	0.0	0.0	0.0
150-154	24.573684210526316	0.0	0.0	0.0	0.0	0.0
155-159	25.047368421052635	0.0	0.0	0.0	0.0	0.0
160-164	24.226315789473684	0.0	0.0	0.0	0.0	0.0
165-169	24.457894736842107	0.0	0.0	0.0	0.0	0.0
170-174	24.910526315789475	0.0	0.0	0.0	0.0	0.0
175-179	23.20263157894737	0.0	0.0	0.0	0.0	0.0
180-184	23.089473684210525	0.0	0.0	0.0	0.0	0.0
185-189	23.41842105263158	0.0	0.0	0.0	0.0	0.0
190-194	23.471052631578946	0.0	0.0	0.0	0.0	0.0
195-199	21.96578947368421	0.0	0.0	0.0	0.0	0.0
200-204	22.160526315789472	0.0	0.0	0.0	0.0	0.0
205-209	21.239473684210527	0.0	0.0	0.0	0.0	0.0
210-214	21.434210526315788	0.0	0.0	0.0	0.0	0.0
215-219	20.63421052631579	0.0	0.0	0.0	0.0	0.0
220-224	20.35263157894737	0.0	0.0	0.0	0.0	0.0
225-229	19.85526315789474	0.0	0.0	0.0	0.0	0.0
230-234	19.747368421052634	0.0	0.0	0.0	0.0	0.0
235-239	19.17105263157895	0.0	0.0	0.0	0.0	0.0
240-244	19.094736842105263	0.0	0.0	0.0	0.0	0.0
245-249	18.757894736842104	0.0	0.0	0.0	0.0	0.0
250-251	18.065789473684212	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1103	1	5.571428571428573
1103	2	-5.571428571428573
1103	3	-6.0
1103	4	-1.8571428571428577
1103	5	7.0
1103	6	8.714285714285715
1103	7	-2.1428571428571423
1103	8	9.285714285714285
1103	9	-4.0
1103	10-14	-2.885714285714286
1103	15-19	-0.9428571428571431
1103	20-24	1.514285714285716
1103	25-29	-4.085714285714282
1103	30-34	-3.9714285714285715
1103	35-39	-5.62857142857143
1103	40-44	-4.428571428571431
1103	45-49	-4.885714285714283
1103	50-54	0.11428571428571033
1103	55-59	-9.257142857142853
1103	60-64	-6.4857142857142875
1103	65-69	-6.114285714285714
1103	70-74	2.171428571428571
1103	75-79	4.457142857142863
1103	80-84	-3.2285714285714278
1103	85-89	-4.828571428571429
1103	90-94	-6.685714285714283
1103	95-99	-8.142857142857139
1103	100-104	-6.399999999999999
1103	105-109	-4.485714285714286
1103	110-114	-5.37142857142857
1103	115-119	-5.314285714285713
1103	120-124	-5.885714285714286
1103	125-129	-6.142857142857142
1103	130-134	-8.942857142857145
1103	135-139	-3.942857142857143
1103	140-144	-6.171428571428571
1103	145-149	-4.857142857142854
1103	150-154	-3.8000000000000043
1103	155-159	-7.085714285714287
1103	160-164	-5.485714285714282
1103	165-169	-7.9714285714285715
1103	170-174	-9.6
1103	175-179	-3.8000000000000007
1103	180-184	-6.314285714285717
1103	185-189	-3.8571428571428577
1103	190-194	-3.0285714285714267
1103	195-199	-2.885714285714286
1103	200-204	-3.3142857142857167
1103	205-209	-3.7714285714285722
1103	210-214	-3.9714285714285715
1103	215-219	-6.428571428571427
1103	220-224	-4.114285714285716
1103	225-229	-3.1428571428571423
1103	230-234	-1.8857142857142861
1103	235-239	-0.3714285714285719
1103	240-244	0.14285714285714235
1103	245-249	-3.8571428571428577
1103	250-251	-9.285714285714285
2112	1	5.571428571428573
2112	2	7.428571428571427
2112	3	-6.0
2112	4	-1.8571428571428577
2112	5	-6.0
2112	6	4.714285714285715
2112	7	-2.1428571428571423
2112	8	5.285714285714285
2112	9	-4.0
2112	10-14	0.31428571428571317
2112	15-19	-1.1428571428571423
2112	20-24	2.914285714285718
2112	25-29	-4.085714285714282
2112	30-34	-4.171428571428571
2112	35-39	-5.828571428571429
2112	40-44	-7.228571428571428
2112	45-49	-2.085714285714282
2112	50-54	-0.28571428571428825
2112	55-59	0.1428571428571459
2112	60-64	-4.085714285714287
2112	65-69	-8.914285714285715
2112	70-74	-2.2285714285714278
2112	75-79	4.457142857142863
2112	80-84	-2.4285714285714306
2112	85-89	-6.828571428571429
2112	90-94	-8.285714285714283
2112	95-99	-7.342857142857138
2112	100-104	-6.0
2112	105-109	-2.485714285714284
2112	110-114	-3.37142857142857
2112	115-119	-2.114285714285714
2112	120-124	-6.685714285714285
2112	125-129	-7.542857142857141
2112	130-134	-4.5428571428571445
2112	135-139	0.25714285714285623
2112	140-144	-6.171428571428571
2112	145-149	-4.857142857142854
2112	150-154	-6.200000000000005
2112	155-159	-3.4857142857142875
2112	160-164	-7.885714285714283
2112	165-169	0.6285714285714299
2112	170-174	-6.0
2112	175-179	-4.600000000000001
2112	180-184	-1.9142857142857181
2112	185-189	-1.8571428571428577
2112	190-194	2.9714285714285715
2112	195-199	0.9142857142857146
2112	200-204	0.6857142857142833
2112	205-209	-6.571428571428573
2112	210-214	-7.171428571428571
2112	215-219	-4.428571428571427
2112	220-224	0.6857142857142833
2112	225-229	1.457142857142859
2112	230-234	-1.8857142857142861
2112	235-239	-0.3714285714285719
2112	240-244	0.34285714285714164
2112	245-249	-1.8571428571428577
2112	250-251	-1.2857142857142847
1113	1	5.571428571428573
1113	2	-5.571428571428573
1113	3	7.0
1113	4	-1.8571428571428577
1113	5	-6.0
1113	6	4.714285714285715
1113	7	-2.1428571428571423
1113	8	5.285714285714285
1113	9	-4.0
1113	10-14	-6.085714285714285
1113	15-19	-4.742857142857142
1113	20-24	-3.485714285714284
1113	25-29	-8.485714285714282
1113	30-34	-6.171428571428571
1113	35-39	-4.428571428571431
1113	40-44	-5.428571428571431
1113	45-49	-8.285714285714283
1113	50-54	-5.68571428571429
1113	55-59	-6.857142857142854
1113	60-64	-4.085714285714287
1113	65-69	-4.114285714285714
1113	70-74	-3.0285714285714285
1113	75-79	4.857142857142861
1113	80-84	-3.2285714285714278
1113	85-89	-3.62857142857143
1113	90-94	-6.685714285714283
1113	95-99	-4.9428571428571395
1113	100-104	-5.199999999999999
1113	105-109	-5.285714285714285
1113	110-114	-5.37142857142857
1113	115-119	-4.5142857142857125
1113	120-124	-2.685714285714287
1113	125-129	0.45714285714285907
1113	130-134	-1.7428571428571438
1113	135-139	2.057142857142857
1113	140-144	-4.171428571428571
1113	145-149	-3.657142857142855
1113	150-154	-5.0000000000000036
1113	155-159	0.914285714285711
1113	160-164	-1.485714285714284
1113	165-169	-3.9714285714285715
1113	170-174	-2.6000000000000014
1113	175-179	-1.8000000000000007
1113	180-184	-2.3142857142857167
1113	185-189	-2.6571428571428584
1113	190-194	-1.2285714285714278
1113	195-199	-2.4857142857142858
1113	200-204	1.6857142857142833
1113	205-209	-2.571428571428573
1113	210-214	-8.37142857142857
1113	215-219	-3.2285714285714278
1113	220-224	-2.7142857142857153
1113	225-229	0.6571428571428584
1113	230-234	2.114285714285714
1113	235-239	-0.7714285714285722
1113	240-244	0.14285714285714235
1113	245-249	-5.457142857142857
1113	250-251	-7.285714285714285
2102	1	-7.428571428571427
2102	2	7.428571428571427
2102	3	-6.0
2102	4	-1.8571428571428577
2102	5	-6.0
2102	6	-8.285714285714285
2102	7	-2.1428571428571423
2102	8	-7.714285714285715
2102	9	9.0
2102	10-14	1.9142857142857146
2102	15-19	0.25714285714285623
2102	20-24	-6.885714285714283
2102	25-29	2.914285714285718
2102	30-34	0.4285714285714306
2102	35-39	5.9714285714285715
2102	40-44	8.17142857142857
2102	45-49	9.314285714285717
2102	50-54	0.7142857142857117
2102	55-59	-5.857142857142854
2102	60-64	4.114285714285714
2102	65-69	3.485714285714284
2102	70-74	4.571428571428573
2102	75-79	2.457142857142859
2102	80-84	-4.0285714285714285
2102	85-89	9.17142857142857
2102	90-94	1.3142857142857167
2102	95-99	-2.342857142857138
2102	100-104	1.6000000000000014
2102	105-109	4.714285714285715
2102	110-114	-3.171428571428571
2102	115-119	-3.914285714285713
2102	120-124	-5.485714285714286
2102	125-129	-5.342857142857142
2102	130-134	-0.3428571428571452
2102	135-139	0.25714285714285623
2102	140-144	8.62857142857143
2102	145-149	0.5428571428571445
2102	150-154	0.399999999999995
2102	155-159	-0.6857142857142868
2102	160-164	0.9142857142857181
2102	165-169	-2.571428571428573
2102	170-174	-2.8000000000000007
2102	175-179	-6.400000000000002
2102	180-184	3.2857142857142847
2102	185-189	-1.257142857142858
2102	190-194	1.571428571428573
2102	195-199	0.31428571428571317
2102	200-204	-1.514285714285716
2102	205-209	6.828571428571429
2102	210-214	3.828571428571429
2102	215-219	-0.42857142857142705
2102	220-224	-0.9142857142857146
2102	225-229	0.8571428571428577
2102	230-234	4.114285714285714
2102	235-239	0.8285714285714274
2102	240-244	-1.6571428571428584
2102	245-249	4.342857142857142
2102	250-251	-1.2857142857142847
2107	1	-7.428571428571427
2107	2	-5.571428571428573
2107	3	7.0
2107	4	11.142857142857142
2107	5	10.0
2107	6	-8.285714285714285
2107	7	-2.1428571428571423
2107	8	5.285714285714285
2107	9	-4.0
2107	10-14	-1.485714285714284
2107	15-19	4.457142857142859
2107	20-24	-6.485714285714284
2107	25-29	0.11428571428571743
2107	30-34	-1.9714285714285715
2107	35-39	-2.2285714285714313
2107	40-44	3.171428571428571
2107	45-49	-5.485714285714284
2107	50-54	0.7142857142857117
2107	55-59	1.9428571428571466
2107	60-64	-3.085714285714287
2107	65-69	1.2857142857142847
2107	70-74	-5.228571428571428
2107	75-79	-4.9428571428571395
2107	80-84	5.771428571428572
2107	85-89	-1.0285714285714285
2107	90-94	16.714285714285715
2107	95-99	9.457142857142863
2107	100-104	-1.0
2107	105-109	3.514285714285716
2107	110-114	9.82857142857143
2107	115-119	-3.314285714285713
2107	120-124	-3.485714285714284
2107	125-129	3.25714285714286
2107	130-134	1.4571428571428555
2107	135-139	-2.1428571428571423
2107	140-144	-1.37142857142857
2107	145-149	-2.4571428571428555
2107	150-154	3.799999999999997
2107	155-159	-1.085714285714289
2107	160-164	1.514285714285716
2107	165-169	3.828571428571429
2107	170-174	3.1999999999999993
2107	175-179	-1.0
2107	180-184	3.085714285714282
2107	185-189	-1.8571428571428577
2107	190-194	-3.428571428571427
2107	195-199	-1.0857142857142854
2107	200-204	5.285714285714285
2107	205-209	-2.7714285714285722
2107	210-214	9.82857142857143
2107	215-219	9.171428571428574
2107	220-224	1.2857142857142847
2107	225-229	0.05714285714285694
2107	230-234	-0.8857142857142861
2107	235-239	3.628571428571428
2107	240-244	8.342857142857142
2107	245-249	2.1428571428571423
2107	250-251	11.714285714285715
2105	1	5.571428571428573
2105	2	7.428571428571427
2105	3	10.0
2105	4	-1.8571428571428577
2105	5	-6.0
2105	6	8.714285714285715
2105	7	14.857142857142858
2105	8	-7.714285714285715
2105	9	13.0
2105	10-14	14.714285714285712
2105	15-19	5.457142857142859
2105	20-24	11.714285714285719
2105	25-29	12.514285714285716
2105	30-34	14.028571428571432
2105	35-39	11.171428571428567
2105	40-44	7.9714285714285715
2105	45-49	12.114285714285717
2105	50-54	3.514285714285709
2105	55-59	10.742857142857144
2105	60-64	5.114285714285714
2105	65-69	14.085714285714282
2105	70-74	6.571428571428573
2105	75-79	-1.542857142857141
2105	80-84	5.9714285714285715
2105	85-89	0.7714285714285687
2105	90-94	11.114285714285717
2105	95-99	15.457142857142859
2105	100-104	12.2
2105	105-109	9.914285714285715
2105	110-114	9.62857142857143
2105	115-119	14.885714285714286
2105	120-124	16.914285714285715
2105	125-129	15.257142857142856
2105	130-134	9.857142857142854
2105	135-139	4.657142857142858
2105	140-144	15.82857142857143
2105	145-149	19.342857142857145
2105	150-154	12.599999999999994
2105	155-159	15.31428571428571
2105	160-164	16.71428571428572
2105	165-169	17.22857142857143
2105	170-174	16.200000000000003
2105	175-179	17.4
2105	180-184	8.885714285714283
2105	185-189	12.942857142857143
2105	190-194	5.371428571428574
2105	195-199	4.114285714285714
2105	200-204	0.08571428571428186
2105	205-209	11.428571428571427
2105	210-214	15.028571428571432
2105	215-219	8.171428571428574
2105	220-224	-0.9142857142857146
2105	225-229	-0.34285714285714164
2105	230-234	-0.8857142857142861
2105	235-239	-0.9714285714285715
2105	240-244	-4.657142857142858
2105	245-249	-3.8571428571428577
2105	250-251	4.214285714285715
1110	1	-7.428571428571427
1110	2	-5.571428571428573
1110	3	-6.0
1110	4	-1.8571428571428577
1110	5	7.0
1110	6	-10.285714285714285
1110	7	-4.142857142857142
1110	8	-9.714285714285715
1110	9	-6.0
1110	10-14	-6.485714285714286
1110	15-19	-3.3428571428571416
1110	20-24	0.7142857142857189
1110	25-29	1.1142857142857174
1110	30-34	1.8285714285714292
1110	35-39	0.9714285714285715
1110	40-44	-2.2285714285714278
1110	45-49	-0.6857142857142833
1110	50-54	0.914285714285711
1110	55-59	9.14285714285715
1110	60-64	8.514285714285712
1110	65-69	0.2857142857142847
1110	70-74	-2.828571428571429
1110	75-79	-9.74285714285714
1110	80-84	1.1714285714285708
1110	85-89	6.37142857142857
1110	90-94	-7.485714285714284
1110	95-99	-2.142857142857139
1110	100-104	4.800000000000001
1110	105-109	-5.885714285714286
1110	110-114	-2.171428571428571
1110	115-119	4.285714285714288
1110	120-124	7.314285714285713
1110	125-129	0.05714285714286049
1110	130-134	4.257142857142856
1110	135-139	-1.1428571428571423
1110	140-144	-6.571428571428571
1110	145-149	-4.057142857142855
1110	150-154	-1.8000000000000043
1110	155-159	-3.885714285714288
1110	160-164	-4.285714285714283
1110	165-169	-7.171428571428571
1110	170-174	1.6000000000000014
1110	175-179	0.1999999999999993
1110	180-184	-4.714285714285717
1110	185-189	-1.457142857142859
1110	190-194	-2.2285714285714278
1110	195-199	1.1142857142857139
1110	200-204	-2.9142857142857164
1110	205-209	-2.571428571428573
1110	210-214	-9.17142857142857
1110	215-219	-2.8285714285714274
1110	220-224	6.685714285714283
1110	225-229	0.45714285714285907
1110	230-234	-0.6857142857142868
1110	235-239	-1.9714285714285715
1110	240-244	-2.6571428571428584
1110	245-249	8.542857142857141
1110	250-251	3.2142857142857153
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
16	5.0
17	11.0
18	2.0
19	8.0
20	6.0
21	10.0
22	2.0
23	3.0
24	3.0
25	0.0
26	2.0
27	0.0
28	3.0
29	4.0
30	2.0
31	0.0
32	2.0
33	5.0
34	4.0
35	1.0
36	0.0
37	2.0
38	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.94736842105263	14.473684210526317	10.526315789473683	21.052631578947366
2	38.15789473684211	34.21052631578947	18.421052631578945	9.210526315789473
3	35.526315789473685	23.684210526315788	26.31578947368421	14.473684210526317
4	28.947368421052634	22.36842105263158	25.0	23.684210526315788
5	32.89473684210527	15.789473684210526	27.631578947368425	23.684210526315788
6	35.526315789473685	25.0	23.684210526315788	15.789473684210526
7	34.21052631578947	40.78947368421053	13.157894736842104	11.842105263157894
8	17.105263157894736	40.78947368421053	27.631578947368425	14.473684210526317
9	26.31578947368421	31.57894736842105	23.684210526315788	18.421052631578945
10-14	36.84210526315789	27.89473684210526	20.0	15.263157894736842
15-19	32.10526315789474	22.36842105263158	20.789473684210527	24.736842105263158
20-24	38.94736842105263	30.0	17.63157894736842	13.421052631578947
25-29	24.210526315789473	39.473684210526315	20.789473684210527	15.526315789473685
30-34	27.89473684210526	26.31578947368421	29.47368421052631	16.315789473684212
35-39	22.105263157894736	34.21052631578947	28.421052631578945	15.263157894736842
40-44	24.210526315789473	30.789473684210527	28.421052631578945	16.57894736842105
45-49	23.42105263157895	36.05263157894737	22.36842105263158	18.157894736842106
50-54	26.31578947368421	25.263157894736842	25.0	23.42105263157895
55-59	38.68421052631579	22.105263157894736	21.052631578947366	18.157894736842106
60-64	31.842105263157894	22.63157894736842	20.526315789473685	25.0
65-69	16.315789473684212	27.631578947368425	31.57894736842105	24.47368421052632
70-74	17.894736842105264	39.473684210526315	23.157894736842106	19.473684210526315
75-79	17.63157894736842	48.421052631578945	19.210526315789473	14.736842105263156
80-84	19.210526315789473	38.68421052631579	21.052631578947366	21.052631578947366
85-89	18.684210526315788	38.68421052631579	22.894736842105264	19.736842105263158
90-94	23.157894736842106	30.526315789473685	24.47368421052632	21.842105263157897
95-99	21.31578947368421	28.947368421052634	28.157894736842103	21.578947368421055
100-104	23.157894736842106	28.157894736842103	23.157894736842106	25.526315789473685
105-109	26.31578947368421	26.31578947368421	25.263157894736842	22.105263157894736
110-114	18.421052631578945	28.157894736842103	26.57894736842105	26.842105263157894
115-119	22.36842105263158	32.10526315789474	23.684210526315788	21.842105263157897
120-124	17.63157894736842	30.789473684210527	25.526315789473685	26.052631578947366
125-129	17.105263157894736	27.89473684210526	30.0	25.0
130-134	20.789473684210527	29.210526315789476	25.789473684210527	24.210526315789473
135-139	20.789473684210527	28.157894736842103	24.210526315789473	26.842105263157894
140-144	20.789473684210527	27.105263157894736	30.0	22.105263157894736
145-149	16.315789473684212	27.105263157894736	29.736842105263158	26.842105263157894
150-154	21.052631578947366	29.736842105263158	26.31578947368421	22.894736842105264
155-159	15.263157894736842	31.842105263157894	28.157894736842103	24.736842105263158
160-164	18.157894736842106	30.0	26.052631578947366	25.789473684210527
165-169	18.421052631578945	25.789473684210527	29.736842105263158	26.052631578947366
170-174	17.36842105263158	32.89473684210527	28.421052631578945	21.31578947368421
175-179	18.157894736842106	30.263157894736842	25.789473684210527	25.789473684210527
180-184	23.684210526315788	26.31578947368421	27.105263157894736	22.894736842105264
185-189	22.36842105263158	29.736842105263158	27.631578947368425	20.263157894736842
190-194	20.263157894736842	31.05263157894737	27.631578947368425	21.052631578947366
195-199	21.052631578947366	31.57894736842105	26.052631578947366	21.31578947368421
200-204	17.63157894736842	31.05263157894737	28.947368421052634	22.36842105263158
205-209	23.684210526315788	24.736842105263158	29.210526315789476	22.36842105263158
210-214	21.31578947368421	29.47368421052631	26.052631578947366	23.157894736842106
215-219	22.105263157894736	26.842105263157894	28.947368421052634	22.105263157894736
220-224	21.578947368421055	27.631578947368425	27.105263157894736	23.684210526315788
225-229	21.052631578947366	33.421052631578945	25.0	20.526315789473685
230-234	23.157894736842106	32.89473684210527	24.736842105263158	19.210526315789473
235-239	23.42105263157895	28.68421052631579	28.947368421052634	18.947368421052634
240-244	21.842105263157897	32.89473684210527	29.47368421052631	15.789473684210526
245-249	22.105263157894736	35.526315789473685	24.736842105263158	17.63157894736842
250-251	22.36842105263158	30.263157894736842	23.026315789473685	24.342105263157894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.3333333333333333
36	0.3333333333333333
37	0.6666666666666666
38	1.6666666666666665
39	2.333333333333333
40	3.3333333333333335
41	2.6666666666666665
42	2.6666666666666665
43	2.6666666666666665
44	3.3333333333333335
45	3.666666666666667
46	2.333333333333333
47	1.6666666666666665
48	1.6666666666666665
49	2.333333333333333
50	3.666666666666667
51	1.6666666666666665
52	0.6666666666666666
53	0.0
54	0.0
55	0.0
56	0.0
57	0.3333333333333333
58	0.0
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	76.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.68421052631578
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.66666666666667	97.36842105263158
2	1.3333333333333335	2.631578947368421
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC	2	2.631578947368421	No Hit
CGGGTCAGAAATTTGAGGAAACAAGGGGGTTACTTGGAGAAATGCGGCAC	1	1.3157894736842104	No Hit
TGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGC	1	1.3157894736842104	No Hit
TTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGAATGGGGCA	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT	1	1.3157894736842104	No Hit
GTGATGGAGAGGTCCGCGCGGGGGAAAAGAGGTGGGATTATCTTTTGCCT	1	1.3157894736842104	No Hit
ACCCACAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG	1	1.3157894736842104	No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAAAGGGTTAAGAATAAGGTAGAATT	1	1.3157894736842104	No Hit
CTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTC	1	1.3157894736842104	No Hit
AAAACAAAACGTTACCATCGCCATTCGGATTGACACCCATATGAGCGTCT	1	1.3157894736842104	No Hit
ATCCCAGTGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCA	1	1.3157894736842104	No Hit
GATTGGGAGAGCGTCGGGGTGGGGAAGAGTGGTAGAGATAATGTTGAGTT	1	1.3157894736842104	No Hit
GAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGA	1	1.3157894736842104	No Hit
TGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAG	1	1.3157894736842104	No Hit
GGAATTATAAGGGGAAGGGAGGGGAATAGTGGAAAAATTAATTGTGAATG	1	1.3157894736842104	No Hit
TGCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATC	1	1.3157894736842104	No Hit
GTGATCGCCTTTTCTTCACAAGTATATAAAGTCGGTTTAAATTAGGAGTA	1	1.3157894736842104	No Hit
GTGACACCTCCTTGGACCCATGAAACGTTCTTTCTCATTTGCGCCGCTGC	1	1.3157894736842104	No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT	1	1.3157894736842104	Illumina Single End PCR Primer 1 (97% over 34bp)
ACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTC	1	1.3157894736842104	No Hit
GGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAACCAGCAATCT	1	1.3157894736842104	No Hit
GTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAA	1	1.3157894736842104	No Hit
TTACCAGAAATGCGATAACAGGTGCATGGTTAGTGTCAATAGTTGGACTT	1	1.3157894736842104	No Hit
GAATCGGAGAGCGGCGTGTAGGGGAAGAGGGTTAAGAATTGGGTAGATAT	1	1.3157894736842104	No Hit
AGCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTT	1	1.3157894736842104	No Hit
GATCGGGAGAGGGGCGTGTAGGGGAAAAGGGGTAAGGATAATGTAGATAT	1	1.3157894736842104	No Hit
GATTGGAAAAGGGGCGGGGAGGGGAAGAGGGTTAAGAATAATGTAGAATT	1	1.3157894736842104	No Hit
ATTTCATCGAGAGTCCTGCCAGAACCAATGCGCGCATTAGGTACAAATGC	1	1.3157894736842104	No Hit
AGTGGCTCTAAATAAGGCTTGAAGAGAGGGCCATCTATGGAAGGAGTGCC	1	1.3157894736842104	No Hit
GATAAGAGGGGCGTTGGGTTGGGGAAGAGCGTTAAAAATTAAAGAGCTTT	1	1.3157894736842104	No Hit
CGGGGGAGCGAAAGCAGGGTGACAAAAACATAACGGACTCCATCACCATG	1	1.3157894736842104	No Hit
GATCTGGAGAGGGGCGGGTAGGGGAAAAGGGGGGAGGATAATGTTAAACT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	1	1.3157894736842104	No Hit
GTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCA	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA	1	1.3157894736842104	No Hit
GAGCTCAGCGGAGCCAATCAAGAAAGGAAGCATCACCCAAGACGATGTCT	1	1.3157894736842104	No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA	1	1.3157894736842104	No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA	1	1.3157894736842104	No Hit
CCGAGAGGAAAGCTTTCCAGAAGAGGGGTCCAGATTGCTTCAAATGAGAA	1	1.3157894736842104	No Hit
GGCCTTGTCTTTAGCCATTCCATGAGAGCCTTAAGAACTGTGTTCTTTCT	1	1.3157894736842104	No Hit
CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGAACATGCATTCAG	1	1.3157894736842104	No Hit
GGAAACGGGACTCTAACTTACTTACTGTCAGCCAGATAGCTGCCAAAAGG	1	1.3157894736842104	No Hit
GAACGGGAAAAGGGCCTGTTAGGGAAAAAGGGTTACATTAAAGTAGAACT	1	1.3157894736842104	No Hit
TCCACGATTTGTTTCCCAACAAGAGTGGCTGGTTCGATATCGAGGCCAAG	1	1.3157894736842104	No Hit
TCGCTTCGTGAAACTGAGGTGAGAATGGGAGACCTCCACTACCTCCAGAG	1	1.3157894736842104	No Hit
AGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGC	1	1.3157894736842104	No Hit
CTCTCCCTATTCCACCATACTTGCATCCAACTCTCTCTTCGATTTCAGCT	1	1.3157894736842104	No Hit
GAATGGGAGAGGGGCGGGGGGGGGAAAAGGGGTAGGGATAGTGGAGAAAA	1	1.3157894736842104	No Hit
AGGGGAATCGGTGTCGTTCCCTTCATCCGGAACAATGATGAACAGAGGCG	1	1.3157894736842104	No Hit
GGATTACTGATCGTCTTTTTTTCAAAAGTATTTATCGTCGCTTTAAATAC	1	1.3157894736842104	No Hit
TGGGGATTACTGATCGTGTTTTTCTTAAAAGTATTTATCGTCGCTTTAAA	1	1.3157894736842104	No Hit
GCTTACGAACAGTTTGCTGATGATAGACAATGGGAAAACAGAAAGATAGT	1	1.3157894736842104	No Hit
CAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGT	1	1.3157894736842104	No Hit
GGGGGGTTGGAAATCAGGCAAGCCATTTGATTGGATGCCAAGCCGCTTCT	1	1.3157894736842104	No Hit
ATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACC	1	1.3157894736842104	No Hit
GGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGA	1	1.3157894736842104	No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGTAAGGATAATGTAGAAAT	1	1.3157894736842104	No Hit
GATTGGGAAAGGGGGGGGGGGGGGAAGAGGGGTAGGGATTGGGGAGAAAT	1	1.3157894736842104	No Hit
GTGTAATCAAGGAAAAGGGGATAAAGTCAATGCGCAAACAGGGAAAGAGG	1	1.3157894736842104	No Hit
GACGGACATCCGCCATGCGAAACGGAGATGTAAGTATGATGCCTAGAGCA	1	1.3157894736842104	No Hit
CATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCAT	1	1.3157894736842104	No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA	1	1.3157894736842104	No Hit
GGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG	1	1.3157894736842104	No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAAAGGGTTAAGAATAGAGTAGAACT	1	1.3157894736842104	No Hit
CTGGTTATTTGTACCATCCCGCATTTCTTTCTTGTATGACAACCTCAGAA	1	1.3157894736842104	No Hit
GGTTTAAATACGGGTTGATAAGAGGGCCTTCTAAGGATAGAGTGCTTGAG	1	1.3157894736842104	No Hit
AAATTTGCTGGCGAAATCTGTATTCAACAGTCTATATACGTCTCCACAAA	1	1.3157894736842104	No Hit
CACACTCACAGTGCCCATTAAGCGTGAACTGCAGCGTACAGACGTTGTCC	1	1.3157894736842104	No Hit
GCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGT	1	1.3157894736842104	No Hit
GAATGGAAAAACGGCGGGGAGGGGAAAAGGGGTAAGAATAATGTAGAACT	1	1.3157894736842104	No Hit
GAGTCGGAAAGCGAGCGGGAGGGGGAGAGAGGTAAAATTATAAGTAAAAG	1	1.3157894736842104	No Hit
GATTGGGAAAGCGGCGGGGTGGGGAAAAGGGGTAGGAATTGTGTAGAACT	1	1.3157894736842104	No Hit
GGTTCGGAAAGCGGCGGGGGGGGGAAGAGGGGAGGGAATATAGGCTAGAT	1	1.3157894736842104	No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGTGTGAATAAAATA	1	1.3157894736842104	No Hit
GAAACCTACTGGGACGTGTTGGGGAAGACAGTTTAGATTACCTTCTAGAC	1	1.3157894736842104	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.0	0.0	0.0
80-84	0.0	0.0	0.0
85-89	0.0	0.0	0.0
90-94	0.0	0.0	0.0
95-99	0.0	0.0	0.0
100-104	0.0	0.0	0.0
105-109	0.0	0.0	0.0
110-114	0.0	0.0	0.0
115-119	0.0	0.0	0.0
120-124	0.0	0.0	0.0
125-129	0.0	0.0	0.0
130-134	0.0	0.0	0.0
135-139	0.0	0.0	0.0
140-144	0.0	0.0	0.0
145-149	0.0	0.0	0.0
150-154	2.6315789473684212	0.0	0.0
155-159	6.578947368421052	0.0	0.0
160-164	6.842105263157895	0.0	0.0
165-169	8.157894736842106	0.0	0.0
170-174	9.210526315789474	0.0	0.0
175-179	11.05263157894737	0.0	0.0
180-184	11.842105263157896	0.0	0.0
185-189	12.894736842105264	0.0	0.0
190-194	14.473684210526315	0.0	0.0
195-199	18.42105263157895	0.0	0.0
200-204	20.789473684210527	0.0	0.0
205-209	23.68421052631579	0.0	0.0
210-214	23.68421052631579	0.0	0.0
215-219	24.736842105263158	0.0	0.0
220-224	25.0	0.0	0.0
225-229	25.0	0.0	0.0
230-234	26.31578947368421	0.0	0.0
235-239	26.31578947368421	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCCCTA	5	0.0	245.00002	3
CTCTCCC	5	0.0	245.00002	1
CCTCTTT	5	0.0	245.00002	245
TATTCCA	5	0.0	245.00002	8
TCTCCCT	5	0.0	245.00002	2
ATTCCAC	5	0.0	245.00002	9
CCCTATT	5	0.0	245.00002	5
TCCCTAT	5	0.0	245.00002	4
CTATTCC	5	0.0	245.00002	7
CCTATTC	10	0.0	122.50001	6
>>END_MODULE