FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated f7.34200000008741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated f7.34200000008741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC22.631578947368421No Hit
CGGGTCAGAAATTTGAGGAAACAAGGGGGTTACTTGGAGAAATGCGGCAC11.3157894736842104No Hit
TGGACTACCACGATTCAAATGTGAAGAACTTATATGAAAAGGTAAGAAGC11.3157894736842104No Hit
TTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGAATGGGGCA11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11.3157894736842104No Hit
GTGATGGAGAGGTCCGCGCGGGGGAAAAGAGGTGGGATTATCTTTTGCCT11.3157894736842104No Hit
ACCCACAATACTCAGAGGAAGCAAAATTAAACAGAGAAGAAATAGATGGG11.3157894736842104No Hit
GATCGGGAGAGCGGCGGGGAGGGGAAAAGGGTTAAGAATAAGGTAGAATT11.3157894736842104No Hit
CTGCACACTGATCTGGCCTGCGGATGCCTTTTGTTGATTGGTATTTCCTC11.3157894736842104No Hit
AAAACAAAACGTTACCATCGCCATTCGGATTGACACCCATATGAGCGTCT11.3157894736842104No Hit
ATCCCAGTGATATTTGCTGCAATGACGAGAGGATCACTTGAATCGCTGCA11.3157894736842104No Hit
GATTGGGAGAGCGTCGGGGTGGGGAAGAGTGGTAGAGATAATGTTGAGTT11.3157894736842104No Hit
GAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGA11.3157894736842104No Hit
TGGAAGAGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAG11.3157894736842104No Hit
GGAATTATAAGGGGAAGGGAGGGGAATAGTGGAAAAATTAATTGTGAATG11.3157894736842104No Hit
TGCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTACCACCAATC11.3157894736842104No Hit
GTGATCGCCTTTTCTTCACAAGTATATAAAGTCGGTTTAAATTAGGAGTA11.3157894736842104No Hit
GTGACACCTCCTTGGACCCATGAAACGTTCTTTCTCATTTGCGCCGCTGC11.3157894736842104No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGCTATAGTGTAGATCT11.3157894736842104Illumina Single End PCR Primer 1 (97% over 34bp)
ACAAAAGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTC11.3157894736842104No Hit
GGTGGTAGTAGCCATCTGTCTGTGAGACCGATGCTGTGAACCAGCAATCT11.3157894736842104No Hit
GTACCATCCATCTACCATCCCTGTCCACCCCCCTTCAATGAAACCGGCAA11.3157894736842104No Hit
TTACCAGAAATGCGATAACAGGTGCATGGTTAGTGTCAATAGTTGGACTT11.3157894736842104No Hit
GAATCGGAGAGCGGCGTGTAGGGGAAGAGGGTTAAGAATTGGGTAGATAT11.3157894736842104No Hit
AGCAAACAATGGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTT11.3157894736842104No Hit
GATCGGGAGAGGGGCGTGTAGGGGAAAAGGGGTAAGGATAATGTAGATAT11.3157894736842104No Hit
GATTGGAAAAGGGGCGGGGAGGGGAAGAGGGTTAAGAATAATGTAGAATT11.3157894736842104No Hit
ATTTCATCGAGAGTCCTGCCAGAACCAATGCGCGCATTAGGTACAAATGC11.3157894736842104No Hit
AGTGGCTCTAAATAAGGCTTGAAGAGAGGGCCATCTATGGAAGGAGTGCC11.3157894736842104No Hit
GATAAGAGGGGCGTTGGGTTGGGGAAGAGCGTTAAAAATTAAAGAGCTTT11.3157894736842104No Hit
CGGGGGAGCGAAAGCAGGGTGACAAAAACATAACGGACTCCATCACCATG11.3157894736842104No Hit
GATCTGGAGAGGGGCGGGTAGGGGAAAAGGGGGGAGGATAATGTTAAACT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG11.3157894736842104No Hit
GTCTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCA11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA11.3157894736842104No Hit
GAGCTCAGCGGAGCCAATCAAGAAAGGAAGCATCACCCAAGACGATGTCT11.3157894736842104No Hit
CAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCTTGCAA11.3157894736842104No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA11.3157894736842104No Hit
CCGAGAGGAAAGCTTTCCAGAAGAGGGGTCCAGATTGCTTCAAATGAGAA11.3157894736842104No Hit
GGCCTTGTCTTTAGCCATTCCATGAGAGCCTTAAGAACTGTGTTCTTTCT11.3157894736842104No Hit
CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGAACATGCATTCAG11.3157894736842104No Hit
GGAAACGGGACTCTAACTTACTTACTGTCAGCCAGATAGCTGCCAAAAGG11.3157894736842104No Hit
GAACGGGAAAAGGGCCTGTTAGGGAAAAAGGGTTACATTAAAGTAGAACT11.3157894736842104No Hit
TCCACGATTTGTTTCCCAACAAGAGTGGCTGGTTCGATATCGAGGCCAAG11.3157894736842104No Hit
TCGCTTCGTGAAACTGAGGTGAGAATGGGAGACCTCCACTACCTCCAGAG11.3157894736842104No Hit
AGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAGTCAGTGAAACGC11.3157894736842104No Hit
CTCTCCCTATTCCACCATACTTGCATCCAACTCTCTCTTCGATTTCAGCT11.3157894736842104No Hit
GAATGGGAGAGGGGCGGGGGGGGGAAAAGGGGTAGGGATAGTGGAGAAAA11.3157894736842104No Hit
AGGGGAATCGGTGTCGTTCCCTTCATCCGGAACAATGATGAACAGAGGCG11.3157894736842104No Hit
GGATTACTGATCGTCTTTTTTTCAAAAGTATTTATCGTCGCTTTAAATAC11.3157894736842104No Hit
TGGGGATTACTGATCGTGTTTTTCTTAAAAGTATTTATCGTCGCTTTAAA11.3157894736842104No Hit
GCTTACGAACAGTTTGCTGATGATAGACAATGGGAAAACAGAAAGATAGT11.3157894736842104No Hit
CAGAATGAGTGCTGACCGTGCCAGGAAAATGAGGTCTTCAATCTCAGCGT11.3157894736842104No Hit
GGGGGGTTGGAAATCAGGCAAGCCATTTGATTGGATGCCAAGCCGCTTCT11.3157894736842104No Hit
ATTCGCTTCTGGTAGGCCTGCAAATTTTCAAGAAGGTCATCTTTCAGACC11.3157894736842104No Hit
GGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGA11.3157894736842104No Hit
GATCGGGAGAGCGGCGGGTAGGGGAAGAGGGGTAAGGATAATGTAGAAAT11.3157894736842104No Hit
GATTGGGAAAGGGGGGGGGGGGGGAAGAGGGGTAGGGATTGGGGAGAAAT11.3157894736842104No Hit
GTGTAATCAAGGAAAAGGGGATAAAGTCAATGCGCAAACAGGGAAAGAGG11.3157894736842104No Hit
GACGGACATCCGCCATGCGAAACGGAGATGTAAGTATGATGCCTAGAGCA11.3157894736842104No Hit
CATGTTGAACATGCCCATCATCATCCCAGGACTCAGTGATGCTGTGCCAT11.3157894736842104No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA11.3157894736842104No Hit
GGACAAAGCGTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTG11.3157894736842104No Hit
GATCGGAAGAGCGGCGGGTAGGGGAAAAGGGTTAAGAATAGAGTAGAACT11.3157894736842104No Hit
CTGGTTATTTGTACCATCCCGCATTTCTTTCTTGTATGACAACCTCAGAA11.3157894736842104No Hit
GGTTTAAATACGGGTTGATAAGAGGGCCTTCTAAGGATAGAGTGCTTGAG11.3157894736842104No Hit
AAATTTGCTGGCGAAATCTGTATTCAACAGTCTATATACGTCTCCACAAA11.3157894736842104No Hit
CACACTCACAGTGCCCATTAAGCGTGAACTGCAGCGTACAGACGTTGTCC11.3157894736842104No Hit
GCTGTGCCATCTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGT11.3157894736842104No Hit
GAATGGAAAAACGGCGGGGAGGGGAAAAGGGGTAAGAATAATGTAGAACT11.3157894736842104No Hit
GAGTCGGAAAGCGAGCGGGAGGGGGAGAGAGGTAAAATTATAAGTAAAAG11.3157894736842104No Hit
GATTGGGAAAGCGGCGGGGTGGGGAAAAGGGGTAGGAATTGTGTAGAACT11.3157894736842104No Hit
GGTTCGGAAAGCGGCGGGGGGGGGAAGAGGGGAGGGAATATAGGCTAGAT11.3157894736842104No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGTGTGAATAAAATA11.3157894736842104No Hit
GAAACCTACTGGGACGTGTTGGGGAAGACAGTTTAGATTACCTTCTAGAC11.3157894736842104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA50.0245.000023
CTCTCCC50.0245.000021
CCTCTTT50.0245.00002245
TATTCCA50.0245.000028
TCTCCCT50.0245.000022
ATTCCAC50.0245.000029
CCCTATT50.0245.000025
TCCCTAT50.0245.000024
CTATTCC50.0245.000027
CCTATTC100.0122.500016
Produced by FastQC (version 0.11.2)