FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated f4.342000000085c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated f4.342000000085c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279446
Sequences flagged as poor quality0
Sequence length251
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG260409.318437193590174No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA172956.189031154498544No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA161695.786091051580628No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT117804.215483492338413No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC107493.8465392240361287No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG92933.325508327190226No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT75562.7039213300601905No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG45111.6142653679065004No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG40401.4457175983911026No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA38231.3680639551111844No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG27120.9704916155536311No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA26010.9307701666869448No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA25130.8992792883061487No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC21660.7751050292364178No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA18340.6562985335270499No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14560.5210308968459022No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7660.2741137822692041No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA6740.2411915003256443No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA6570.23510803518389956No Hit
GGGGGGAGCGAAAGCAGGGGTCAGGATATGCAGCCGACCTGAAGAGCACA4800.17176842753161614No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3750.1341940840090751No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3630.12989987332078468No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA3340.11952219749074955No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT3180.11379658323969569No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3150.11272303056762308No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG3020.10807096898864182No Hit
GGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAC2860.10234535473758793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA100.0014756053245.000021
CGGGTTA82050.0244.25351
GGGTTAT82050.0243.5072
GGTTATT82150.0243.210573
TTATTAG82400.0242.770025
GTTATTA82350.0242.768684
TATTAGT82700.0242.185616
ATTAGTA83300.0240.294137
GGGGGGA60350.0240.128431
TAGTAGA84250.0239.183999
GGGGGAG63850.0225.238862
GAGCGAA48850.0224.437066
CGAAAGC49700.0224.049279
GCGAAAG49100.0224.042768
AGCGAAA49050.0223.771657
GGAGCGA49000.0223.55
GGGGAGC64900.0221.217273
TTAGTAG91350.0219.789288
GGGAGCG50100.0218.59284
CGAGAGG9750.0207.3077245
Produced by FastQC (version 0.11.2)