FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated e7.34200000008734.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated e7.34200000008734.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA25.405405405405405No Hit
GATTGGGAAAGGGGCGGGGTGGGGAAGAGTGGCGGCTTCTGGGTAGATTT12.7027027027027026No Hit
GATCGGGTGAACGGCGGGGAGGGGAAAAGGGGCTTCGCCCGTGGAGAGTT12.7027027027027026No Hit
ATGAAAACAGAATGGTGCTGGCTAGCACTACGGCAAAGGCTATGGAACAG12.7027027027027026No Hit
GGAATATGATGCCGTTGCAACTACACATTCCTGGATTCCCAAGAGGAATC12.7027027027027026No Hit
GATCCGAGAAAAGGTGTGCTGGGGAAAAGGGGGCACCACCCTTGAGATCT12.7027027027027026No Hit
GATTTGGAGAAGGGTGCGTAGGGGAAGAGGGGAAGCTCCTAGGTAGGGTT12.7027027027027026No Hit
AATTTAAATAAAAAAGTTGATGATGGTTTCCTGGACATTTGGACTTACAA12.7027027027027026No Hit
GATTGGGAGAGCGGGGGGGGGGGGAAGAGGGTATTCCCCCGTGTAGAGCT12.7027027027027026No Hit
GATAGGGGGGAAAGCGGGGGAAGGAAAAGGCAAAGAGAAAGCGGCAAAGA12.7027027027027026No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG12.7027027027027026No Hit
AATGGGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCC12.7027027027027026No Hit
CATTCCCATTTAGGGCATTTTGGACAAAGCGTCTACGCTGCAGTCCTCGC12.7027027027027026No Hit
GAAATGGAAGGGGTCCTGCTGGGATAAACAAGGATTTCCCCGGTTGTGGT12.7027027027027026No Hit
AAGCGACGATAAATACATTTGAAAAAAAGACGATCAGTAATCCACAATAT12.7027027027027026No Hit
CAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACTTAT12.7027027027027026No Hit
CCAGAACTGGGGAATTGAATCCATCGACAATGTGATGGGAATGATCGGAA12.7027027027027026No Hit
AAGGGCAACAGAGAAAAATCTGGCGAAGACCAACCAACAGTGTAAAGGGT12.7027027027027026No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA12.7027027027027026No Hit
GGCTCCATTCCGGTGAGAACAAGCGCTCTTGTTCTCTGATATGTGGCATC12.7027027027027026No Hit
CTGTGCTAATTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAAAACGG12.7027027027027026No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTATTCTAACCGAGG12.7027027027027026No Hit
GGACATACTTATGAGGATGTCAAAAATGCAGATGGGGTCCTCATCGGAGG12.7027027027027026No Hit
GATCGGGGAAGGGGCGGGGAGGGGAAAAGGGGTAGCTCTTGTGGAGAAAT12.7027027027027026No Hit
TCTCGTGTCATTTCCTCGAGGGTCATGTCAGAAAGGTAGCGCGAAGTAGG12.7027027027027026No Hit
GATCGGCAGAGCGGCGCGGAGGGGAAGAGGGGGTCCATCCGTGCAGTAAC12.7027027027027026No Hit
GGAAGCGAAGGTAATGGTGAAATTTCTCCAACTATTGCTCCCTCCTCAGT12.7027027027027026No Hit
GAGTTGAATAGGGGCGGGGAGGGGAAGAGTGTCATGCTACGGGTAGATAT12.7027027027027026No Hit
GATCCGGAGAAGGTGGGGAGGGGAAAAGATGCATCGCCCGCGTCAAAGTC12.7027027027027026No Hit
ATTGATGCCATCATGACAAGCACTTGCTGACCAAGCGACTGACTCAAATC12.7027027027027026No Hit
GAATTGGAGAGCGGCGTGTTGGGGAAAAGGGGGATGGCCTGGGGATAGCT12.7027027027027026No Hit
ATAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATC12.7027027027027026No Hit
GATCGGGAGGGCGGCGTGGTGGGGAAAAGGGGATTCCACTGGGTAGAAAT12.7027027027027026No Hit
GCGTAACTACGTCATAAGAATTTTGAAAAGCGAAAAATCAAAGCAAATTT12.7027027027027026No Hit
GCTCTGAAGAGGGTCGCGTACGGGAAAAGGGGACCCGTCTCCGTTTGTTC12.7027027027027026No Hit
AACGACCCCGATCGTGCGTTCCTTTGACATGAGTAATGTAGGGAGTTCTT12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)