FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated e5.34200000008635.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated e5.34200000008635.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78278
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA11550.0237.571432
TTGGGAG8850.0236.69063
ATTGGGA11800.0232.538132
TCGGGAG9650.0232.301453
TTGGAAG3750.0231.929113
GAGCGGC6600.0230.147329
GATTGGG12450.0229.252851
GATTGGA4950.0227.67261
GGAGAGG14700.0227.495856
TGGGAGA10700.0226.67814
AAGCGGC3050.0224.913949
AGAGCGG8000.0223.558438
GAGAGCG6550.0222.55327
CGGGAGA11850.0219.152134
ATTGGAA5050.0218.312852
GAGAGGG15700.0214.566157
GAACGGG3200.0214.37111
GATCGGG13000.0213.899931
TGGAAGA5550.0209.680864
GAACGGC1350.0208.69999
Produced by FastQC (version 0.11.2)