FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated e4.342000000085bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated e4.342000000085bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78942
Sequences flagged as poor quality0
Sequence length251
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG52526.652985736363407No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA40415.118948088470017No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA37754.78199184211193No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT31343.9700032935572955No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC28303.584910440576626No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG25263.1998175875959567No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT24103.0528742621164904No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13091.6581794228674216No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11211.4200298953662183No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC9651.2224164576524537No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA8761.1096754579311392No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT8191.0374705479972637No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA7160.9069950089939448No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG6410.811988548554635No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA5140.651110942210737No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3860.4889665830609815No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3160.4002938866509589No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG2210.27995237009449975No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA1900.24068303311291836No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA1710.21661472980162652No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC1630.2064807073547668No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT1110.1406095614501786No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA1030.1304755390033189No Hit
GGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACT990.12540852777988903No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA900.11400775252717185No Hit
GGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAAC850.10767398849788451No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA830.1051404828861696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAC100.0014736045245.00002245
AGCTTAC100.0014736045245.00002245
CGGGTTA18750.0244.346661
GGGTTAT18700.0244.344922
ATTAGTA18900.0243.055577
TTATTAG18800.0243.045215
TATTAGT18900.0242.407426
GGTTATT18800.0242.393633
GGGGGGA17300.0240.043351
GTTATTA19000.0239.842094
TAGTAGA19550.0233.721249
GGGGGAG18500.0223.81082
CGAGAGG2850.0214.91228245
TTAGTAG21450.0214.160868
GGAGCGA14500.0210.362095
GAGCGAA14500.0210.362096
AGCGAAA14700.0209.166677
GGGGAGC19800.0209.116173
GCGAAAG14800.0208.581098
CGAAAGC14900.0208.003379
Produced by FastQC (version 0.11.2)