FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated e3.34200000008536.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated e3.34200000008536.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102699
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA23000.0240.206512
TCGGGAG19750.0236.936723
ATTGGGA11500.0236.478262
GAGCGGC11450.0233.231459
CGGGAGA24950.0232.725464
ACGGGAG6300.0229.444433
TTGGGAG10100.0228.019793
GAGAGGG34500.0227.601447
GAGAGCG11750.0227.276617
GGAGAGG34300.0226.428576
AACGGGA8300.0225.813252
AAGCGGC2950.0224.237299
GATTGGG12950.0222.29731
AGAGCGG13150.0219.84798
GAACGGG8850.0218.700581
GATCGGG26050.0218.666031
ATCCGGA2300.0218.369552
TGGGAGA14250.0216.631584
GGGAGAG43750.0215.885
AATGGGA5350.0215.233662
Produced by FastQC (version 0.11.2)