FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated e2.342000000084bd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated e2.342000000084bd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences289120
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG123024.254980630879912No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA75172.5999584947426673No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT72322.5013835085777534No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT57671.9946734919756501No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA54981.9016325401217486No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG51681.7874930824571114No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA51521.7819590481460985No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC48501.6775041505257333No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC41451.433660763696735No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA39041.3503043718871057No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG36451.2607221914775872No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA25680.8882125069175428No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC23850.8249169894853349No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG21740.7519369120088545No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT17480.6045932484781406No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA17320.5990592141671278No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG16050.5551328168234643No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA15950.5516740453790813No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG13650.4721223021582734No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10000.34587714443829554No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA9550.33031267293857225No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA8120.28085224128389596No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7100.24557277255118984No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCCAAATT6160.21306032097399003No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA3100.10722191477587162No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA46450.0240.78041
TCCGGAG1800.0238.194463
GGGTTAT47000.0237.962772
TATTAGT47450.0236.480516
TTATTAG47550.0235.210315
GGTTATT47500.0235.23
ATTAGTA47850.0234.759667
GTTATTA48400.0230.573354
ATCCGGA2100.0227.52
TAGTAGA49850.0225.832499
TTAGTAG50450.0221.932628
CCGGAGA2300.0218.369584
GGAGCGA36050.0215.097095
GAGCGAA36300.0214.290656
AGCGAAA36800.0213.709247
GCGAAAG37550.0212.05068
GGGGGAG49300.0211.952332
CGAAAGC37700.0211.856759
GGGGGGA49850.0210.842511
GATCCGG2300.0207.71741
Produced by FastQC (version 0.11.2)