FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated d7.34200000008727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated d7.34200000008727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG42.185792349726776No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA42.185792349726776No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA31.639344262295082No Hit
GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT31.639344262295082No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA31.639344262295082No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA21.092896174863388No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC21.092896174863388No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT10.546448087431694No Hit
GGTTCAGACAGCGCTCGGGAAGGGGAGAAAGCTCAAAGCCCTCTATTGTT10.546448087431694No Hit
CTGGGCCGTTGCAGGTCCAAGGTCATTGTTTATCATGTTGTTCTTTATCA10.546448087431694No Hit
GAAGGGATGAGGGGCGGGGAGGGGAAGAGTGATGAAGGACGAGTTAGATT10.546448087431694No Hit
ATTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGGCGATCTGAACT10.546448087431694No Hit
GTCTACGCTGCAGTCCTCGCTCACTGGGCACGGTGAGCGTGAACACAAAT10.546448087431694No Hit
ACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAA10.546448087431694No Hit
GTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCA10.546448087431694No Hit
CAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGTCTTATTGC10.546448087431694No Hit
GATTGGAAGAGCGGCGGGGAGGGGAAAAGGGGGTAGAGGCGGGGGGGGAT10.546448087431694No Hit
ATCATATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCAT10.546448087431694No Hit
TGACTCGAACAAAGGTGTAACGGCAGCATGTCCTCATGCTGGAGCAAAAA10.546448087431694No Hit
ATCTAGAAAATTGCTTCTCATTGTTCAGGCACTTAGGGACAACCTGGAAC10.546448087431694No Hit
CGGTAGATCGACAGGAAGGGAGATGGTTTATATGGTAAAATAAGCTGAAC10.546448087431694No Hit
TGAATGGGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGC10.546448087431694No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC10.546448087431694No Hit
AAATTTAGGCTTCTCTACCCCTACATATACTGTTCACCACTGTCCAGGCC10.546448087431694No Hit
GAACTGAAGAGCGGCGTGGTGGGGAAAAGGGGTTAGAGGCGGGGGGAGTT10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGAACTGGTACACTGTCAGGGCACAGGAGG10.546448087431694No Hit
GTTGCCTCTTCCTTTTAAGGACTTTTGATCTCGGCGGAGCCGATCAAGGA10.546448087431694No Hit
GACCGGAAGAGGGGCGGGTGGGGGAAAAGGGGGCAGAGGCGGGGGGAGAA10.546448087431694No Hit
AATGTAGTCCAGAACATTTTTATTTTACAATTTTAAACCATAAATCAAAA10.546448087431694No Hit
GGTCCGAAGAGCGGCTGGGTGGGGAAAAGGGTACGATTTAACGTTAATTG10.546448087431694No Hit
CATTCTGAGTGCGGGGTTCTTCTCTTGCCTTCCTGATGTGTACTTTTTGA10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTCGGTCTCAGACTGTGTCCCTTGTGTGTTGGA10.546448087431694No Hit
GAATCTCCCGATTCCACCAATCATTCTTCCGACAGATGCTCTGATTTCTG10.546448087431694No Hit
GGGCGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA10.546448087431694No Hit
AATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAATTGG10.546448087431694No Hit
GAACGGGAGAGTGGCGGGGCGGGGAAAAGGGGAAAGAACCATGTAAAATT10.546448087431694No Hit
GTCAATGAGGAAAATTGCATCGTACTCTGTTCTGGAGTCGCAGCAGCATA10.546448087431694No Hit
TAGGACAGCTCATTAGGGTTCGATATGGGCTCCTGTCTTTAATGGTTCCA10.546448087431694No Hit
AATCAACAAAAGGCATCCGCAGGCCAGATCAGTGTGCAGCCTACATTCTC10.546448087431694No Hit
ATTTTGGGCAAAGAAGACAAGAGATATGGCCCAGCATTAAGCATCAATGG10.546448087431694No Hit
GAACAGATTGCTGATTCACAGCATCGGTCTCACAGACAGATGGCTACTAC10.546448087431694No Hit
GGTCGGGAGGGGGGCGGGGAGGGGAAAAGGGGGTAGAGGCGGGGAGAGAA10.546448087431694No Hit
TTAGCTGCTCTATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATT10.546448087431694No Hit
GAAATAGATGGGGTAAAGCTGGAATCAACAAGGATTTACCAGATTTTGGC10.546448087431694No Hit
ATTCCAACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTCTAGGG10.546448087431694No Hit
GGCGTGAAAAGCGATGCGGAGGGGAAAAGGGTTCGGGGCCGCGAGATTAT10.546448087431694No Hit
ATATGAATGGTTCCCTTATGACAAACACATCCCCCTTGGAACCGATTCTT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGG10.546448087431694No Hit
GATCGGGAGAGGGGCGGGGTGGGGAAGAGGGGTCAGAGCCGGGGGGGGAT10.546448087431694No Hit
ACTGCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTT10.546448087431694No Hit
CAATGGCGAAGATGCAACAGCAGGTCTTACTCATATCATGATTTGGCATT10.546448087431694No Hit
GATTAACTCCTAGGGAGAAATCTCGCGTGTATTGATGAAGTTAGTATACT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA10.546448087431694No Hit
TGATGGATCAAGTAAGAGAAAGTCGAAACCCAGGAAACGCTGAGATTGAA10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGGAAAATTCAAGAAGCTTTAGTAGCATCT10.546448087431694No Hit
AATGAGAATGTGGAAACCATGGACTCCAATACCCTGGAACTGAGAAGCAG10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGGTAGTGGGAGAGGGAGAGGGAGAGGGAGAAG10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG10.546448087431694No Hit
GGCCTGCACAGCGTTTGCGAGGGGGATCAGTTCATTGATGCTTAATGCAT10.546448087431694No Hit
ATGTCCTCATGCTGGAGCAAAAAGCTTCTACAAAAATTTAATATGGCTAG10.546448087431694No Hit
GAGTTGGAGAGGGGCGGGGTGGGGAAAAGGGGTTCGAGCCGGTTTAGGAA10.546448087431694No Hit
GAGCCGAACAGTTGCGTTTGGGGAGAGAGGGGATACAGCCCGGTCAAAGG10.546448087431694No Hit
GGGCATCACCCAATCCATTGTCTGCAAATCGCTTGCGGATATGCCAAAGG10.546448087431694No Hit
GATCGGGAGAGAGTCGGGTATGGGAAAAGGGGTTGAGGCCCCGGACGGCT10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA10.546448087431694No Hit
ATATCATTGGGATCTTGCACCTGATATTGTGGATTACTGATCGTCTTTTT10.546448087431694No Hit
CTCTTTCAGGACATACTTATGAGGATGTCAACAATGCAGTTGGGGTCCTC10.546448087431694No Hit
GCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACT10.546448087431694No Hit
GATTGGAAGAAGGCGGGGAGGGGAAAAGGGGTCAGAAGCCGGGGAGAAGC10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA10.546448087431694No Hit
TAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCGATCA10.546448087431694No Hit
CCTGTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGC10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT10.546448087431694No Hit
GATTGGAAGAGGGGCGGGGAGGGGAAGAGGGGGTAGGGACGTGGGGTATC10.546448087431694No Hit
GGGCTTCTTTATAAAATACATCCAAATACATAACCACTGTACTTCCCTTC10.546448087431694No Hit
CGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACTACCTCCAGAGC10.546448087431694No Hit
ACAAGGCAGACAACAGTGTTAGAAGATGGTAATACAAGAGAAAGCGCAAA10.546448087431694No Hit
CGGGTTATCAGTAGCAACAAAGAGTATTCTGAACAAATTACTTTTCAATG10.546448087431694No Hit
GAAACAAACTTCCAAATGTGTGCATATTGCAGCAAACTTGTTAGTTTCGA10.546448087431694No Hit
ATTACTGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACGG10.546448087431694No Hit
TTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTCTGACATG10.546448087431694No Hit
CGAATGCATATCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATT10.546448087431694No Hit
TATGTGTACCATCTGTTGTACGTTATGATTACGACCATAGGGTTCACAGT10.546448087431694No Hit
ATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCT10.546448087431694No Hit
ATTATTGATTCACCCCGTTCGTCGATGAAATGGAAATCCGAATACATGAA10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGCACATTAGAAAAACAAGCTGTAGAATCA10.546448087431694No Hit
AGACTCCCCGCCCCTGGAAGGACAAATCTTCTGGCTTTGCACTTTCCATC10.546448087431694No Hit
GGTTTGGAAAGGGGCGGGGCGGGGAAAAGGGGCTAGGGTCGTGGTAGGAT10.546448087431694No Hit
AAAAGGAGAGAAGGCTGGGCAAGGGGACGTAGTGTTGGTAATGAAACGAA10.546448087431694No Hit
GATCGGAAGAGGGGCGGGTAGGGGAAGAGGGGTCAGAGACGGGGAGATAT10.546448087431694No Hit
GTTTCATAAGAGGAAAGAAAGTGATTCCAAGAGGAAAGCTTTCCACAAGA10.546448087431694No Hit
ATCTCGGCGGAGCCGATCACGGAATGGGGCATCACCCAATCCATTGTCTG10.546448087431694No Hit
AAAGCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAA10.546448087431694No Hit
AGACATAATAACACTCCATCAGTAGTTCTTTTGTCAAATGCATATGAGAA10.546448087431694No Hit
TCCCTTTGTTCACCTTTCTCTTTCCCCAGGAAGCACCTCGGACTGTCCCT10.546448087431694No Hit
CTAGTGTCCAGTAATAGTTCATTCTCCCTTCTTGATCCCTCACTTTGGGT10.546448087431694No Hit
AGAGAGAGGCAAGTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGA10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGCAAACCACTTGAATGGATGTCAATCCGACTCT10.546448087431694No Hit
CGGGTTAGTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT10.546448087431694No Hit
GGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACT10.546448087431694No Hit
GAGACACTTAGAATGACAATTGCATCTGTACCTACTTCGCGCTACCTTTC10.546448087431694No Hit
GGGGGAGCGAAAGCAGGCAGAGCAGCGGGGGGAGGGGGAGAGGCGGGCTC10.546448087431694No Hit
AGAGAAGAACCCCGCACTCAGAATGAAGTGGCCCAGCATTAAGCATCAAT10.546448087431694No Hit
TTTTTAATTGACCTCAGTTCTCCCTTTAAATTTTCATCTTCTCCCCTCCA10.546448087431694No Hit
GGAAATTGGAAACGGCTGCTTTGAATTTTACCACAAATGCGATAACACGT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGCAAGGGCCCCGAGCCATGGAAAACAGTCGACA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGTCGGTCTCAGACTGTGTCCCTCGTGTGTTGGA10.546448087431694No Hit
GCGTAGACGCTTTGTCCAAAATGCCCTAAATGGGAATGGGGACCCGAACA10.546448087431694No Hit
ATTTGAGGAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAG10.546448087431694No Hit
AAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCTTTC10.546448087431694No Hit
AACTCACAGTAAGGGGAACCCTGGGGTGTGTGAGAAACAAGCTATCATAT10.546448087431694No Hit
ACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGAC10.546448087431694No Hit
ATCTTTATTTCTCTGTATCTACTGTATCTTACTTTTTCCTCAGGCCACAT10.546448087431694No Hit
ACTTGGGAATCAAAATCAGATTGAAACATGCAATCAAAGCGTCATTACTT10.546448087431694No Hit
AGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG10.546448087431694No Hit
GATTGGAGAGGCGTTGTGTAGGGAAAAAAAGGTTAAAGACGTGAAGGGTG10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC10.546448087431694No Hit
TGCAAGATTGCTCAGTTCATTGATGCTTATGGTCCACAGTGGTCTTAGTG10.546448087431694No Hit
GATTGGCAGAGCGGAGGGGTGGGAAAGAGGGGTCAGAGGCGTGGCGAATC10.546448087431694No Hit
AGTGTCATCATTTGAAAGGTTTGAGATATTCCCCAAGACAAGTTCATGGC10.546448087431694No Hit
AACATATGTCTGATTTACCCAAGTGTTGTTTTCATAAGTAATGACGCTTT10.546448087431694No Hit
CGGGTTATTAGCAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA10.546448087431694No Hit
TAAGCTGCTGCCTTCAGCTGGGTTGTGATCCCAGGATCCTGGGATCGAGT10.546448087431694No Hit
GATAGGGAGAGGGTCGTGTAGGGGAAGAGGGTTCAGAGCCGCGTTGGAAA10.546448087431694No Hit
GATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTT10.546448087431694No Hit
AGCTGCCCAGAGGGCAATGATGGATCAAGTAAGAGAAAGTCGAAACCCAG10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
TTCGAGTCTGGACGGATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGAT10.546448087431694No Hit
ATCAATGAACTGAGCAATCTTGCAAAAGGAGAGAAGGCTAATGTGCTAAT10.546448087431694No Hit
GAACGGGAGAGGGGCGGGTTGGGGAAAAGGGTCAGAAGCGGGGAGGAGCA10.546448087431694No Hit
CATTGCTATTGTTCCAACTCCTTTCACCGCAGCACCTGCGGCACCAGACC10.546448087431694No Hit
AAATTCCAATTGTTAGCCAATTGATGCCATCATGACAAGCACTTGCTGAC10.546448087431694No Hit
AGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTGGTGCCAT10.546448087431694No Hit
GTCACTAAGCTATTCAACTGGTGCACTTGCCAGTTGCATTGGCCTCATAT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA10.546448087431694No Hit
AAAGACAAGACCAATCTTGTCACCTCTGACTAAGGGAATTTTAGGATTTG10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG10.546448087431694No Hit
GATCGGGAGGGGGGCGTGGTGGGGAAAAGGGGGCAGAGACGGGGCGAAAA10.546448087431694No Hit
TGGGTTATTAGTAGAAACAAGGCCGTTTTTAAACAATTCGTCGCTACTTG10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGAGAAGCAGCAGAGGGAGAGAGGGAAGCAGGCT10.546448087431694No Hit
AAAAGAGAAGAAGGCTGACGAACTAATTTGGCGAGTAAACGAAGTGTTTG10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGAGTACTTAGGGTGCCTATGTGGCTCAGT10.546448087431694No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10.546448087431694No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT10.546448087431694No Hit
ACAGTAAAGCAAGAACCATTTACACATGCACATTCAGACTCTTGTGTTCT10.546448087431694No Hit
CACTTCTTATTTTTAAGGACTCTTTGGATCCCTCTTTCCAGATCCATTCC10.546448087431694No Hit
GCCAGTTATCCCTGCACACACATGTGATTTCACTAGAATCAGGATAACAG10.546448087431694No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA10.546448087431694No Hit
ATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGC10.546448087431694No Hit
CGGAGTCCTCGCGCACTGTCTTCTCTTTACCCTTTCACTAATCGTATCAT10.546448087431694No Hit
TTTGAGACTATTTAAGAGTGTGGTATAAGGAAATGGTCCAGTTTCATTCT10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGAAAAGAGAGGTGGGGTTTCTTTTTCAGATAGA10.546448087431694No Hit
GGGGGGAGCGAAAGCAGGGGGAGTAGCAGAGGGAGAGGGAGAAGCAGGAT10.546448087431694No Hit
GGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTAC10.546448087431694No Hit
ATTTTCCTTTGAGGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTT10.546448087431694No Hit
TTAGCCATTCCATGAGAGCCTCAAGATCTGTGTTCTTTCCTGCAAAGACA10.546448087431694No Hit
CACTTCTGAATCCATCTTCTCAACATGCAAAAAGCTGGAAGAGTCTTTGA10.546448087431694No Hit
GAACGGGAGAGGGGCGGGGAGGGGAAGAGGGGTGGGAAACGGGGAGGAAT10.546448087431694No Hit
GATTGGTAAAACGTCGTGGGGGGGAAAAGGGGGTAGTACCGAGGTCTCTA10.546448087431694No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC10.546448087431694No Hit
CTGTGTTCTCTTCAGGTCGGCTGCATATCCTGACCCCTGCTCATTTTGAT10.546448087431694No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers