##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename 000000000-AE52Y l01n02 flu mrt-pcr updated d3.34200000008529.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 377415 Sequences flagged as poor quality 0 Sequence length 251 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.038668309420665 32.0 29.0 33.0 29.0 34.0 2 29.790842971265054 32.0 30.0 33.0 16.0 34.0 3 29.39202734390525 33.0 30.0 34.0 16.0 34.0 4 29.731107666626922 33.0 31.0 34.0 16.0 34.0 5 29.74289310175801 32.0 29.0 33.0 16.0 34.0 6 32.06834121590292 33.0 32.0 35.0 29.0 37.0 7 30.286215439237974 33.0 31.0 35.0 16.0 37.0 8 31.33006902216393 34.0 32.0 37.0 16.0 37.0 9 31.143078573983544 34.0 32.0 37.0 16.0 37.0 10-14 32.59222765390883 36.8 34.0 37.4 16.0 37.4 15-19 33.70191009896268 38.0 35.8 38.0 16.6 38.0 20-24 34.20510684524992 38.0 34.8 38.4 20.4 38.4 25-29 33.699157691135746 38.0 34.4 39.0 16.8 39.0 30-34 33.68109640581323 38.0 34.2 39.0 14.8 39.0 35-39 33.513878356716084 38.0 34.0 39.0 15.4 39.0 40-44 33.50582038339759 38.0 34.0 39.0 14.2 39.0 45-49 33.25545354583151 38.0 34.0 39.0 14.2 39.0 50-54 33.68306135156261 38.0 34.0 39.0 15.0 39.0 55-59 33.682487977425374 38.0 33.8 39.0 14.2 39.0 60-64 32.965244094696814 38.0 33.0 39.0 12.0 39.0 65-69 33.13592305552244 38.0 33.0 39.0 12.8 39.0 70-74 33.3904661976869 38.0 33.2 39.0 14.4 39.0 75-79 34.493269742856015 38.0 33.2 39.0 21.0 39.0 80-84 34.00017063444749 38.0 32.8 39.0 16.0 39.0 85-89 33.1837860180438 38.0 33.0 39.0 14.0 39.0 90-94 33.0958043532981 38.0 33.0 39.0 14.0 39.0 95-99 33.02554641442444 38.0 33.0 39.0 14.0 39.0 100-104 32.93576990845621 38.0 32.8 39.0 14.0 39.0 105-109 32.97030430693004 38.0 33.0 39.0 14.0 39.0 110-114 32.865976709987685 38.0 32.6 39.0 14.0 39.0 115-119 32.666221533325384 38.0 31.4 39.0 14.0 39.0 120-124 32.57726481459401 38.0 30.8 39.0 14.0 39.0 125-129 32.488930752619794 38.0 30.4 39.0 14.0 39.0 130-134 32.34771167017739 37.6 29.4 39.0 14.0 39.0 135-139 32.182053177536666 37.2 27.8 39.0 14.0 39.0 140-144 31.901927586343948 37.0 26.0 39.0 14.0 39.0 145-149 31.740462355762226 37.0 26.0 39.0 14.0 39.0 150-154 31.54484161996741 37.0 25.8 38.6 14.0 39.0 155-159 31.294517705973533 37.0 24.6 38.0 14.0 39.0 160-164 31.017623570870263 37.0 24.0 38.0 14.0 39.0 165-169 30.643814898718915 37.0 24.0 38.0 14.0 39.0 170-174 30.17460196335599 37.0 20.8 38.0 13.2 39.0 175-179 29.681525111614537 36.8 15.2 38.0 13.0 39.0 180-184 29.336445027357154 36.4 15.0 38.0 13.0 39.0 185-189 28.777703058966914 34.6 14.6 37.4 13.0 39.0 190-194 28.378145012784337 33.8 14.0 37.0 13.0 39.0 195-199 27.747701071764503 33.0 14.0 37.0 13.0 39.0 200-204 26.914891564988142 31.0 14.0 37.0 12.8 38.0 205-209 26.38387875415657 28.4 14.0 37.0 12.2 38.0 210-214 25.37158830465138 25.6 14.0 37.0 12.0 38.0 215-219 24.307936886451255 24.2 14.0 37.0 12.0 37.8 220-224 23.545487063312272 24.0 14.0 36.4 12.0 37.0 225-229 23.097129684829696 20.4 13.4 35.4 12.0 37.0 230-234 22.024045679159546 15.0 13.0 33.2 12.0 37.0 235-239 21.740773419180478 15.0 13.0 32.8 12.0 37.0 240-244 21.174546321688332 14.0 13.0 31.4 12.0 37.0 245-249 20.85655525085118 14.0 13.0 29.2 12.0 37.0 250-251 19.4964455572778 14.0 13.0 25.0 12.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.004813523243655027 1101 2 0.2963993587674558 1101 3 0.08730798030494569 1101 4 0.18755303879440532 1101 5 0.31269591201025904 1101 6 0.6566837806578008 1101 7 0.371183120440687 1101 8 0.42639959932434834 1101 9 0.21073048124380733 1101 10-14 0.1650649171434253 1101 15-19 0.13644133944436732 1101 20-24 0.09329010744322375 1101 25-29 0.17256040081785073 1101 30-34 0.10690640839404608 1101 35-39 0.017481228655782388 1101 40-44 0.11294783267764785 1101 45-49 0.06308746748272398 1101 50-54 0.06614930269023489 1101 55-59 0.11111781767949225 1101 60-64 0.06914574718324928 1101 65-69 0.15587948495745962 1101 70-74 0.19658784247484107 1101 75-79 0.21648603464684157 1101 80-84 0.17462943682288312 1101 85-89 0.16352373338950343 1101 90-94 0.011751392746347733 1101 95-99 0.22585547116622706 1101 100-104 0.05760508386009633 1101 105-109 0.15991515811306556 1101 110-114 0.12743042716085995 1101 115-119 0.24651321234733814 1101 120-124 0.2925774182948686 1101 125-129 0.21149029399689567 1101 130-134 0.139934980099369 1101 135-139 0.08407828962709374 1101 140-144 0.1448344218601072 1101 145-149 0.3832615450403303 1101 150-154 0.24906468300239837 1101 155-159 0.049700147208778134 1101 160-164 0.26089763268310406 1101 165-169 0.3340170397822533 1101 170-174 0.13493828647917283 1101 175-179 0.08949061704363004 1101 180-184 0.23601360791299797 1101 185-189 0.3285224458984004 1101 190-194 0.12418357654460266 1101 195-199 0.11552349315480015 1101 200-204 0.07430977024720065 1101 205-209 0.048187108702492765 1101 210-214 0.21621387619281052 1101 215-219 0.20685812798768666 1101 220-224 0.09298617905515982 1101 225-229 0.12107365720441976 1101 230-234 0.5312665576228461 1101 235-239 0.2445355238388771 1101 240-244 0.27150412850391703 1101 245-249 0.009404418678570892 1101 250-251 0.01720041358088764 1102 1 0.156804522413875 1102 2 0.37227098981561113 1102 3 0.20243616165854306 1102 4 0.5269215011572363 1102 5 0.34173698308843115 1102 6 0.8511832706924523 1102 7 0.49260985901282694 1102 8 0.740044460749619 1102 9 0.49789919244385317 1102 10-14 0.3375242007768833 1102 15-19 0.26538947391996714 1102 20-24 0.4527932524092293 1102 25-29 0.4427001507299906 1102 30-34 0.4407141999745505 1102 35-39 0.343182404055824 1102 40-44 0.4209487900755846 1102 45-49 0.30897744865821153 1102 50-54 0.3942618702633425 1102 55-59 0.49439073862732386 1102 60-64 0.26414506794822046 1102 65-69 0.2875374437591631 1102 70-74 0.35066163543105944 1102 75-79 0.44416518558148965 1102 80-84 0.5357347139400019 1102 85-89 0.404371634337636 1102 90-94 0.39295792979053346 1102 95-99 0.35655430697894985 1102 100-104 0.3953433821499317 1102 105-109 0.35252874669502177 1102 110-114 0.4145210802399504 1102 115-119 0.37901838208063054 1102 120-124 0.5085642389789768 1102 125-129 0.4917597023292828 1102 130-134 0.413571173191869 1102 135-139 0.48598963543966534 1102 140-144 0.5262973431744946 1102 145-149 0.4351372040600303 1102 150-154 0.5195114902743114 1102 155-159 0.6341111641853416 1102 160-164 0.4843422383709921 1102 165-169 0.7560438771130116 1102 170-174 0.544811776259376 1102 175-179 0.7592221790469971 1102 180-184 0.7523055452853669 1102 185-189 0.7992340470170873 1102 190-194 0.8398657118007833 1102 195-199 0.6483872558581112 1102 200-204 0.8693364760435571 1102 205-209 0.8317864937252537 1102 210-214 0.9107819366210101 1102 215-219 0.7630526436499849 1102 220-224 0.9820968555516885 1102 225-229 0.7946449456162412 1102 230-234 1.2129608501079225 1102 235-239 0.8912647376297187 1102 240-244 0.8928525933249318 1102 245-249 0.6060507226696679 1102 250-251 0.4367725170747434 1103 1 0.18731325382946906 1103 2 0.1348622844384586 1103 3 0.08983270153364487 1103 4 0.17225443039670196 1103 5 0.33632760327292033 1103 6 0.6659410918296942 1103 7 0.2210523759484886 1103 8 0.3631838859403196 1103 9 0.26920482827275194 1103 10-14 0.11729809318261886 1103 15-19 0.14456853730436592 1103 20-24 0.258554151205054 1103 25-29 0.22210054087977227 1103 30-34 0.06865387615876983 1103 35-39 0.1386828757358174 1103 40-44 0.26723084190489743 1103 45-49 0.016877554946283624 1103 50-54 0.13400869976275942 1103 55-59 0.10766586252335486 1103 60-64 0.09625644418665047 1103 65-69 0.14019315151392675 1103 70-74 0.09856554077065027 1103 75-79 0.5099022229189103 1103 80-84 0.35135842002146234 1103 85-89 0.14318620220619493 1103 90-94 0.03760724318667741 1103 95-99 0.014037371319517433 1103 100-104 0.15360610577106115 1103 105-109 0.17383118107601092 1103 110-114 0.19050637919115587 1103 115-119 0.18110039037023995 1103 120-124 0.2202862337798308 1103 125-129 0.16508651893753523 1103 130-134 0.14633244335566076 1103 135-139 0.24746682619046823 1103 140-144 0.22387021880038205 1103 145-149 0.323464724986831 1103 150-154 0.3963701442148633 1103 155-159 0.28212327846422625 1103 160-164 0.34846638625228366 1103 165-169 0.5200995140090576 1103 170-174 0.49146348860714895 1103 175-179 0.4813589107528564 1103 180-184 0.6392175993770373 1103 185-189 0.7602542844364706 1103 190-194 0.6764708537529849 1103 195-199 0.6240955228503289 1103 200-204 0.6746824671904825 1103 205-209 0.404123209210443 1103 210-214 0.5376168998470483 1103 215-219 0.7596518522520626 1103 220-224 0.9269286514785904 1103 225-229 0.796053369265973 1103 230-234 0.7921559207318651 1103 235-239 0.6798170001996695 1103 240-244 0.8349031847390798 1103 245-249 0.88406403215577 1103 250-251 0.6336268810751022 2112 1 -0.0957121833283594 2112 2 0.3568205211765658 2112 3 0.20274194716313332 2112 4 0.44333173999124753 2112 5 0.41410693320951353 2112 6 0.5286823843964612 2112 7 0.18334664677626478 2112 8 0.4225544938344328 2112 9 0.24798835756909554 2112 10-14 0.26910793405909317 2112 15-19 0.24200903926541173 2112 20-24 0.3556101206301321 2112 25-29 0.3207222593968311 2112 30-34 0.31744344110168043 2112 35-39 0.2310666575482685 2112 40-44 0.27894671437574203 2112 45-49 0.30154498655473105 2112 50-54 0.19303444403476533 2112 55-59 0.29178326118100273 2112 60-64 0.23501275975499425 2112 65-69 0.3401884594918556 2112 70-74 0.2864729202549938 2112 75-79 0.23194758197590204 2112 80-84 0.2120131490465198 2112 85-89 0.2858704867048161 2112 90-94 0.3080346010877193 2112 95-99 0.4011857095972502 2112 100-104 0.4122095680568023 2112 105-109 0.2693328115536744 2112 110-114 0.24386170815522235 2112 115-119 0.4897299786879614 2112 120-124 0.3886767209183546 2112 125-129 0.39139447340785694 2112 130-134 0.25543272679644247 2112 135-139 0.3089043764430883 2112 140-144 0.3783663489485427 2112 145-149 0.2813924294576857 2112 150-154 0.4398540985657533 2112 155-159 0.26401516574479444 2112 160-164 0.2279631029031961 2112 165-169 0.3330841519022556 2112 170-174 0.48958209439411604 2112 175-179 0.41742403175371123 2112 180-184 0.24721451117342141 2112 185-189 0.22546176778512006 2112 190-194 0.2894904685379771 2112 195-199 0.2226949542625647 2112 200-204 0.2150978391078766 2112 205-209 0.06780770485006116 2112 210-214 0.16506352276415726 2112 215-219 -0.019983918871098183 2112 220-224 0.10466564657969712 2112 225-229 0.052573787026560126 2112 230-234 0.586949589061355 2112 235-239 0.2773527288813611 2112 240-244 0.2593111901818226 2112 245-249 0.16245577760930985 2112 250-251 0.27188397615893223 1113 1 0.006988756860948797 1113 2 0.10676255995077355 1113 3 -0.11616865704162294 1113 4 0.07737355888332331 1113 5 0.2591577855835965 1113 6 0.6314007923524443 1113 7 0.24861204567124418 1113 8 0.37308313197323884 1113 9 0.19467938030154386 1113 10-14 0.036944905804716655 1113 15-19 -0.03763286093117557 1113 20-24 -0.1172425350526538 1113 25-29 0.051744695419813524 1113 30-34 -0.04262736157924962 1113 35-39 0.025674728503389588 1113 40-44 -0.003269299437555162 1113 45-49 0.06110276622160882 1113 50-54 0.014199448793405622 1113 55-59 0.16383303478816202 1113 60-64 0.043318841607884906 1113 65-69 -0.04199727685919896 1113 70-74 0.01105557296297377 1113 75-79 0.22446661390168288 1113 80-84 0.040193420633251264 1113 85-89 0.03587879712714681 1113 90-94 0.020567882476498767 1113 95-99 0.07896967461089588 1113 100-104 0.09642675273980217 1113 105-109 0.027013917887906302 1113 110-114 0.18886132174705494 1113 115-119 0.1410184117445965 1113 120-124 -0.03281043180391663 1113 125-129 0.11347584779291253 1113 130-134 0.10546400997857575 1113 135-139 0.21029586932311872 1113 140-144 -0.0030418071222548804 1113 145-149 0.21016925174274803 1113 150-154 -0.0060069405863885095 1113 155-159 0.19175249711467757 1113 160-164 0.25212483536688524 1113 165-169 0.2070025530828623 1113 170-174 0.3344039602268474 1113 175-179 0.20711720249213528 1113 180-184 0.09170417184352075 1113 185-189 0.3008640822112767 1113 190-194 0.275171557933767 1113 195-199 0.272162928435673 1113 200-204 0.3908216663061417 1113 205-209 0.4905823907743212 1113 210-214 0.4818722782440119 1113 215-219 0.04063897110208359 1113 220-224 0.23768628924529978 1113 225-229 0.489449301134691 1113 230-234 1.0793007186965298 1113 235-239 0.9305675919393614 1113 240-244 0.7064433534218075 1113 245-249 0.44576950822670725 1113 250-251 0.3648172588180998 2113 1 0.11107208932666879 2113 2 0.19752352570783316 2113 3 0.2139701262877196 2113 4 0.27554532537751086 2113 5 0.6117465683923697 2113 6 0.39678825083717584 2113 7 0.28519441508685617 2113 8 0.5398927812147711 2113 9 -0.09424085832665696 2113 10-14 0.16212788532558164 2113 15-19 0.226925229210309 2113 20-24 0.18100606722325807 2113 25-29 0.33532557601201773 2113 30-34 0.2474285473838833 2113 35-39 0.271604410228349 2113 40-44 0.3890033947081406 2113 45-49 0.18678736470780422 2113 50-54 0.28601050356753177 2113 55-59 0.27442950117511344 2113 60-64 0.20272581096727293 2113 65-69 0.2975910582614105 2113 70-74 0.1905872703257785 2113 75-79 0.07752120062304613 2113 80-84 0.18288787867028589 2113 85-89 0.2735905900661635 2113 90-94 0.2918323412423405 2113 95-99 0.23717808906602045 2113 100-104 0.35875764634619145 2113 105-109 0.26498037251003126 2113 110-114 0.28877095120702023 2113 115-119 0.32608749106869084 2113 120-124 0.25801050334377607 2113 125-129 0.1722039969667719 2113 130-134 0.25385451898960554 2113 135-139 0.3046851914399582 2113 140-144 0.28002391974633056 2113 145-149 0.31941161761849557 2113 150-154 0.2536124203647887 2113 155-159 0.3923057563885948 2113 160-164 0.2773734064087492 2113 165-169 0.10045922623927694 2113 170-174 0.0904409829964763 2113 175-179 0.11352892983962803 2113 180-184 0.051423065324975425 2113 185-189 -0.023874540783239695 2113 190-194 0.0141743585875993 2113 195-199 0.2749246438101203 2113 200-204 0.4537528784999161 2113 205-209 0.5385374970221761 2113 210-214 0.546947604379632 2113 215-219 0.5868918212098393 2113 220-224 0.7383823922760406 2113 225-229 0.5816445348174959 2113 230-234 0.823912323190072 2113 235-239 0.6739804333015833 2113 240-244 0.7193084535269634 2113 245-249 0.23794787551840102 2113 250-251 0.13305794846608876 1112 1 -0.2598932587387175 1112 2 0.21208207943791635 1112 3 0.08964265416799222 1112 4 0.17375810186119267 1112 5 0.40684561809243647 1112 6 0.44447743473361356 1112 7 0.21762108120381995 1112 8 0.48125968768959027 1112 9 0.3010200103423024 1112 10-14 0.08798903022055526 1112 15-19 0.27582527249946764 1112 20-24 0.15660397285290628 1112 25-29 0.3191232713800787 1112 30-34 0.21325444254168247 1112 35-39 0.1429913121681139 1112 40-44 0.1300425404688923 1112 45-49 0.17287052110531675 1112 50-54 0.017229814651614106 1112 55-59 0.12022694917659749 1112 60-64 0.17795885089635988 1112 65-69 0.05889715378614113 1112 70-74 0.1068369868849075 1112 75-79 -0.007585476213137099 1112 80-84 -0.02531211720692994 1112 85-89 0.14768677443672118 1112 90-94 -0.053965931314401416 1112 95-99 0.25679343403932364 1112 100-104 0.10184931627672 1112 105-109 0.01279715633226175 1112 110-114 0.17487863812338134 1112 115-119 0.020624812500940948 1112 120-124 0.11358779509203742 1112 125-129 0.07630265500309719 1112 130-134 0.027283432019643783 1112 135-139 -0.011622450125877037 1112 140-144 0.10262250563406283 1112 145-149 0.2080074000183636 1112 150-154 0.0632438441655836 1112 155-159 0.04855464839247148 1112 160-164 0.0371299564695633 1112 165-169 -0.05927011349810485 1112 170-174 -0.007028663063181284 1112 175-179 -0.09360161787120092 1112 180-184 -0.11888593675702808 1112 185-189 -0.2206808321398377 1112 190-194 0.11487378758308964 1112 195-199 0.20967573119236604 1112 200-204 0.19732608869966484 1112 205-209 0.21619184345906461 1112 210-214 -0.04775112374388968 1112 215-219 0.05894846815589361 1112 220-224 0.2981041048773072 1112 225-229 0.3919671420612154 1112 230-234 0.7895211541768532 1112 235-239 0.6202536496854982 1112 240-244 0.44089489783856095 1112 245-249 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95-99 0.11798843465457765 2110 100-104 0.23071399537739268 2110 105-109 0.25957497903340254 2110 110-114 0.18266864286963624 2110 115-119 0.230359901361318 2110 120-124 0.18152950409409385 2110 125-129 0.13190894466505654 2110 130-134 0.03064623898784191 2110 135-139 -0.01501953557598057 2110 140-144 -0.1449116410075213 2110 145-149 -0.05590219268295016 2110 150-154 -0.04468898758860007 2110 155-159 0.019323013748348217 2110 160-164 0.11605791892143813 2110 165-169 -0.015943430776218293 2110 170-174 0.2659286909895897 2110 175-179 0.17161495181959552 2110 180-184 0.09531590197589779 2110 185-189 0.16387803016613134 2110 190-194 0.32752166846975683 2110 195-199 0.1023177232116339 2110 200-204 0.1755799076741269 2110 205-209 -0.06586820540974614 2110 210-214 0.21478381393958657 2110 215-219 0.34148885523906003 2110 220-224 0.23455443623596395 2110 225-229 0.003923300761794479 2110 230-234 0.4075608644797377 2110 235-239 0.1548371221836078 2110 240-244 0.3380735150973422 2110 245-249 -0.1845581932462217 2110 250-251 0.10775671705857803 2109 1 -0.14773207695713353 2109 2 -0.06482317093935563 2109 3 0.01713916130763593 2109 4 -0.026532951895486434 2109 5 -0.2770010060499537 2109 6 -0.12698734926876298 2109 7 0.017552300378923746 2109 8 0.23313797469184294 2109 9 -0.04080562004095256 2109 10-14 -0.08022464887545055 2109 15-19 -0.1345828055339382 2109 20-24 -0.20330972281938386 2109 25-29 -0.23496159920649262 2109 30-34 0.050247977789972254 2109 35-39 -0.06930603196636298 2109 40-44 -0.17462648275727588 2109 45-49 0.022280340725181702 2109 50-54 0.013139562153867246 2109 55-59 -0.11811507773726504 2109 60-64 -0.02854066796078314 2109 65-69 -0.05362547715038346 2109 70-74 -0.13851261130159997 2109 75-79 -0.4807399650499775 2109 80-84 -0.1261298447918051 2109 85-89 -0.15649432472176272 2109 90-94 0.03034084623399025 2109 95-99 -0.08415903507388123 2109 100-104 0.009646413919654151 2109 105-109 -0.1025354780702088 2109 110-114 -0.12883154380674 2109 115-119 -0.1623763133948728 2109 120-124 -0.3252311767907088 2109 125-129 -0.17197104316640832 2109 130-134 -0.09602013575691615 2109 135-139 -0.1528952505925929 2109 140-144 -0.21438002816971746 2109 145-149 -0.01736562024560584 2109 150-154 -0.1784540196462352 2109 155-159 0.030386930817943636 2109 160-164 -0.14927178382523465 2109 165-169 -0.18827764602099606 2109 170-174 0.06266611798990951 2109 175-179 -0.12422458485425025 2109 180-184 -0.16859563646790932 2109 185-189 -0.18593898089155658 2109 190-194 -0.09634502155066116 2109 195-199 0.1387314856394397 2109 200-204 -0.09351282208761802 2109 205-209 -0.18807439753430444 2109 210-214 0.09177180212135738 2109 215-219 -0.1851184053196775 2109 220-224 -0.1810164477803866 2109 225-229 -0.13804131830316635 2109 230-234 0.18434235632359375 2109 235-239 -0.003986908827716462 2109 240-244 0.07150656280813195 2109 245-249 -0.13970423870307158 2109 250-251 -0.1341574302452173 2108 1 0.2803587935013212 2108 2 0.32592395195164414 2108 3 0.423207801381718 2108 4 0.4769090847153947 2108 5 0.15327000351993902 2108 6 0.6079743901835712 2108 7 0.762799499093326 2108 8 0.3536695111738588 2108 9 0.2650296684598956 2108 10-14 0.06206549471620093 2108 15-19 0.08253944543518799 2108 20-24 -0.11396922499116613 2108 25-29 -0.057946066075693636 2108 30-34 0.09182780311360261 2108 35-39 0.12224626052400822 2108 40-44 -0.014998438361089939 2108 45-49 0.03844104713407859 2108 50-54 0.20739093720987256 2108 55-59 0.03710644480823788 2108 60-64 0.17289680540733343 2108 65-69 0.17659490660254562 2108 70-74 0.11274631668808155 2108 75-79 0.30156521636977374 2108 80-84 0.308278758598739 2108 85-89 0.11184464683610429 2108 90-94 0.38703466901940686 2108 95-99 0.09461952195194812 2108 100-104 0.35349165806236726 2108 105-109 0.22445286818961563 2108 110-114 0.13912260234965146 2108 115-119 0.20787992358468443 2108 120-124 0.14619504703652808 2108 125-129 0.2326299094984563 2108 130-134 0.283288089554496 2108 135-139 0.27644212204282326 2108 140-144 0.18306261848802663 2108 145-149 0.4727839680880841 2108 150-154 0.1575577153950718 2108 155-159 -0.004693531364623027 2108 160-164 0.06379544299283424 2108 165-169 0.021672806411210388 2108 170-174 0.20915209238985355 2108 175-179 0.13757846687922282 2108 180-184 0.13706759344768216 2108 185-189 0.3394047759015528 2108 190-194 0.2853502283292606 2108 195-199 0.21404090567399692 2108 200-204 -0.008878680105844694 2108 205-209 0.027962454370186407 2108 210-214 0.46566721656402166 2108 215-219 0.18016070265112205 2108 220-224 0.44046458224164553 2108 225-229 0.3698638626553077 2108 230-234 0.6914841138005592 2108 235-239 0.29319574715947994 2108 240-244 0.358715356247302 2108 245-249 0.2008628972999027 2108 250-251 0.27128213504161636 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 154.0 15 7465.0 16 32524.0 17 17794.0 18 7602.0 19 4356.0 20 3100.0 21 2673.0 22 2671.0 23 2807.0 24 3468.0 25 4237.0 26 5370.0 27 6963.0 28 9342.0 29 12115.0 30 15266.0 31 19617.0 32 26578.0 33 36758.0 34 29933.0 35 31278.0 36 38383.0 37 44373.0 38 12584.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.321251142641394 11.327848654663963 3.435210577216062 27.915689625478585 2 61.81020892121405 25.47937946292543 8.4548838811388 4.255527734721726 3 59.478293125604445 13.198998449982117 21.5804883218738 5.742220102539645 4 59.927665832041654 11.203317303233842 13.76389385689493 15.105123007829576 5 50.742021382298 10.239126690778056 31.15085515943987 7.867996767484069 6 53.211186624802934 9.914285335770968 29.810155929149612 7.064372110276486 7 22.22036749996688 62.711603937310386 7.817654306267636 7.250374256455096 8 36.111442311513855 26.19053296768809 30.34670058158791 7.351324139210154 9 22.474994369593155 13.658439648662613 29.998277757905754 33.868288223838476 10-14 38.793900613383144 41.187340195805675 12.311116410317554 7.707642780493621 15-19 35.1905462157042 33.42061126346329 10.659671714160805 20.7291708066717 20-24 45.24923492706967 27.449359458421103 16.08001801730191 11.221387597207318 25-29 31.40272644171535 37.02163401030696 18.45867281374614 13.11696673423155 30-34 34.963104275134796 27.03893591934608 21.558549607196323 16.4394101983228 35-39 34.07967356888306 31.820091941231798 17.77030589669197 16.32992859319317 40-44 31.632923969635545 25.39893750910801 21.0882980273704 21.87984049388604 45-49 27.996025595167122 26.80630075646172 22.67874885735861 22.518924791012545 50-54 27.728574645946768 24.084681318972486 24.96440258071354 23.22234145436721 55-59 38.33642012108687 22.46550879005869 20.165335241047654 19.03273584780679 60-64 33.98502974179616 22.427937416371897 20.857305618483633 22.729727223348302 65-69 26.67329067472146 23.368175615701546 26.067008465482296 23.891525244094698 70-74 24.760171164368135 31.71500867745055 22.912761813918365 20.612058344262945 75-79 21.291787554813666 35.88177470423804 23.14544996886716 19.68098777208113 80-84 21.15936038578223 35.02372719685227 20.941457016811732 22.87545540055377 85-89 21.22962786322748 33.38961090576686 20.57851436747347 24.802246863532186 90-94 23.41565650543831 29.967224408144883 22.47806790933058 24.13905117708623 95-99 22.31347455718506 26.628088443755548 25.779420531775365 25.279016467284027 100-104 23.89592358544308 25.79187366691838 24.554296994025147 25.757905753613397 105-109 24.351549355484018 26.098750712080864 24.628485884238835 24.921214048196283 110-114 23.09473656319966 26.534769418279613 24.370997443132893 25.999496575387838 115-119 24.633679106553792 27.34305737715777 24.017063444749148 24.00620007153929 120-124 19.3583823421869 29.44171770688378 23.887139561034704 27.31276038989462 125-129 20.64374759879708 26.82421207424188 24.894241087397162 27.637799239563876 130-134 21.068690963528212 26.515851251275123 23.88495422810434 28.530503557092324 135-139 21.222326204483764 24.552031345882565 24.669514814999303 29.55612763463436 140-144 22.577692990474677 24.266126147609395 24.820582117827854 28.335598744088074 145-149 19.973090615418368 22.936726381689322 27.207889038797628 29.882293964094682 150-154 21.477960058800026 24.023329238810984 27.509573021513727 26.98913768087526 155-159 20.17031649510486 23.403203370295298 29.85652398553317 26.569956149066677 160-164 20.945325437515734 26.032351655339614 28.09411390644251 24.928209000702147 165-169 21.493687320323783 24.561927851304265 25.930076970973598 28.014307857398357 170-174 23.69868712160354 25.587748234701852 26.11825179179418 24.595312851900427 175-179 25.270445144175586 27.084205494856057 24.661671985306352 22.983677375662005 180-184 24.498628298958227 24.61388616126668 26.108157977990253 24.77932756178484 185-189 25.892956976227204 23.906918280320898 26.930648681847497 23.2694760616044 190-194 27.060334740804805 26.7923534449136 24.14804522561937 21.999266588662223 195-199 26.21847367935757 27.980142803090924 23.814805354744966 21.98657816280655 200-204 24.829179989761926 24.402116716143617 27.16178591830527 23.606917375789184 205-209 27.337175258005114 25.44212604162527 24.58429050249725 22.636408197872367 210-214 27.546084573259975 25.54791174061007 23.696632988425467 23.209370697704486 215-219 27.293774757230103 25.38961090576686 24.150179510618287 23.166434826384748 220-224 27.22607975420135 25.209782557431538 24.197421085458803 23.366716602908312 225-229 29.597196506337326 25.64594711177198 22.66137946895564 22.09547691293505 230-234 27.429092568226032 27.03843465515113 22.415878982954023 23.11659379366882 235-239 26.21078982594217 27.23237138554185 22.428055285590283 24.128783502925693 240-244 26.918340245268602 31.285100637282902 21.202719130251385 20.59383998719711 245-249 26.11588098823894 31.65493245097967 20.44477323093848 21.784413329842923 250-251 26.853101828361126 32.440989946067255 17.429378044563737 23.276530181007885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.3333333333333333 3 0.3333333333333333 4 0.3333333333333333 5 1.6666666666666665 6 1.0 7 1.3333333333333333 8 0.6666666666666666 9 0.6666666666666666 10 1.0 11 1.6666666666666665 12 1.0 13 3.0 14 2.6666666666666665 15 3.3333333333333335 16 0.6666666666666666 17 1.0 18 2.6666666666666665 19 2.333333333333333 20 5.333333333333332 21 4.0 22 3.666666666666667 23 8.666666666666664 24 19.666666666666664 25 30.666666666666625 26 34.99999999999997 27 62.00000000000016 28 213.66666666666836 29 385.3333333333289 30 696.3333333333426 31 915.0000000000341 32 868.3333333333621 33 1195.3333333333408 34 2349.6666666666174 35 2743.333333333463 36 2186.6666666665433 37 2168.3333333332016 38 2127.333333333183 39 2398.999999999973 40 3987.333333334029 41 4179.000000000669 42 5279.666666666335 43 6262.666666665441 44 6690.999999998385 45 7413.666666664394 46 8595.333333331086 47 8521.666666664285 48 9603.666666666253 49 11341.000000002747 50 10037.000000000375 51 9148.99999999876 52 8462.666666664178 53 7547.666666664272 54 7849.999999997331 55 7896.666666663955 56 8454.999999997497 57 7448.333333331029 58 5263.66666666635 59 5053.999999999874 60 8712.999999997966 61 6977.999999998124 62 2610.000000000069 63 1042.0000000000423 64 607.9999999999992 65 368.66666666666316 66 248.33333333333601 67 163.3333333333336 68 110.33333333333285 69 91.66666666666644 70 69.33333333333343 71 52.33333333333343 72 51.33333333333342 73 46.00000000000005 74 41.000000000000014 75 47.66666666666673 76 48.3333333333334 77 41.33333333333335 78 43.333333333333364 79 34.99999999999997 80 18.333333333333336 81 11.000000000000002 82 7.666666666666663 83 6.333333333333331 84 4.666666666666666 85 5.666666666666665 86 3.666666666666667 87 3.0 88 2.6666666666666665 89 1.0 90 1.6666666666666665 91 1.3333333333333333 92 1.0 93 0.6666666666666666 94 1.0 95 0.6666666666666666 96 0.0 97 0.3333333333333333 98 0.3333333333333333 99 0.3333333333333333 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 5.2992064438350364E-5 125-129 0.0 130-134 0.0 135-139 5.2992064438350364E-5 140-144 0.0 145-149 5.2992064438350364E-5 150-154 5.2992064438350364E-5 155-159 0.0 160-164 0.0 165-169 0.0 170-174 0.0 175-179 5.2992064438350364E-5 180-184 1.0598412887670073E-4 185-189 1.0598412887670073E-4 190-194 1.5897619331505108E-4 195-199 5.2992064438350364E-5 200-204 5.2992064438350364E-5 205-209 0.0 210-214 5.2992064438350364E-5 215-219 0.0 220-224 1.0598412887670073E-4 225-229 5.2992064438350364E-5 230-234 1.0598412887670073E-4 235-239 5.2992064438350364E-5 240-244 5.2992064438350364E-5 245-249 5.2992064438350364E-5 250-251 1.3248016109587588E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 251 377415.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.033100949280644 #Duplication Level Percentage of deduplicated Percentage of total 1 86.84377432436571 36.503131329926106 2 6.229825804870829 5.2371779390513815 3 2.354345004106831 2.968812636811709 4 1.2374353877235489 2.0805298628158466 5 0.7393761615302903 1.5539136418547161 6 0.5100708690624204 1.2863916198352814 7 0.3343664650553931 0.9838121565809219 8 0.2976734373554897 1.0009710113826131 9 0.2258410046283293 0.854351797142657 >10 0.9369223546475973 7.159088852618396 >50 0.10187462325120811 2.9816568707027042 >100 0.1415324831188451 13.271549364947901 >500 0.02919264451373357 8.393605937801546 >1k 0.0165001903578038 11.591690894859088 >5k 0.0012692454121387537 4.1333160836691185 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT 9843 2.608004451333413 No Hit GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT 5652 1.497555741027781 No Hit GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT 3470 0.9194123180053786 No Hit CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG 2791 0.7395042592371792 No Hit CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG 2658 0.7042645363856762 No Hit CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC 2564 0.6793582660996516 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA 2166 0.5739040578673343 No Hit CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC 2109 0.5588013195024045 No Hit CGGGTTATTAGTAGAAACAAGGGCTGGAGAATAGAACCGAGTCCAGAAAT 2018 0.5346899301829552 No Hit GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 1884 0.49918524700926037 No Hit GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 1823 0.4830226673555635 No Hit GGGGGGAGCGAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT 1656 0.43877429354954095 No Hit CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC 1555 0.412013301008174 No Hit GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 1471 0.38975663394406684 No Hit CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT 1467 0.3886967926552999 No Hit GGGGGGAGCGAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC 1445 0.38286766556708135 No Hit GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA 1401 0.37120941139064423 No Hit GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA 1382 0.36617516526900096 No Hit CGGGTTATTAGTAGAAACAAGGTTGGTTTGATTTAGTTAAAAGCGAAGTT 1354 0.35875627624763196 No Hit CGGGTTATTAGTAGAAACAAGGGAATAGAAACAGCTGTAAAAGGGCGCCT 1221 0.3235165533961289 No Hit CGGGTTATTAGTAGAAACAAGGTCAACATCACCCACAAAGAGTAAATTGT 1190 0.31530278340818463 No Hit CGGGTTATTAGTAGAAACAAGGAGGGAGGGGTGCCTGGGTGGCTCAGTCC 1182 0.3131831008306506 No Hit CGGGTTATTAGTAGAAACAAGGTGTACTGCAGTAGAACTGAAAACGTATT 1178 0.3121232595418836 No Hit CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT 1147 0.3039094895539393 No Hit CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA 1120 0.296755560854762 No Hit GGGGGGAGCAAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT 1103 0.29225123537750225 No Hit GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 1086 0.28774690990024243 No Hit CGGGTTATTAGTAGAAACAAGGGCACCTAGGTGGCTCAGTCATTATGCAT 1014 0.2686697667024363 No Hit GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG 980 0.2596611157479168 No Hit CGGGTTATTAGTAGAAACAAGGTAAGCTAATGAACGTAACTGTGATAAAG 938 0.24853278221586317 No Hit CGGGTTATTAGTAGAAACAAGGGGTCAGCATGTAAGTGGCATGAAATATG 901 0.23872925029476835 No Hit GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 870 0.23051548030682406 No Hit CGGGTTATTAGTAGAAACAAGGCTTCCAGGCTGCCAGGGTGGCTCAGTCG 840 0.2225666706410715 No Hit CGGGTTATTAGTAGAAACAAGGCACACCATATTGACTTCATTTCATTTTT 838 0.222036749996688 No Hit CGGGTTATTAGTAGAAACAAGGAGTACTTAGGGTGCCTATGTGGCTCAGT 831 0.22018202774134574 No Hit GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC 825 0.2185922658081952 No Hit CGGGTTATTAGTAGAAACAAGGAATGATCTAATATAAGATATGCTCTAGA 770 0.20401944808764888 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC 767 0.20322456712107362 No Hit CGGGTTATTAGTAGAAACAAGGGAACGCTATCCAGCAGTTAACAAAAAGA 759 0.2011048845435396 No Hit CGGGTTATTAGTAGAAACAAGGACCCCTTTGAGCTCTGCTGCCAACAAGA 751 0.1989852019660056 No Hit GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA 743 0.19686551938847158 No Hit CGGGTTATTAGTAGAAACAAGGGGACAGAGAGATTTAATATATAATCTAG 737 0.19527575745532105 No Hit GGGGGGAGCGAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC 731 0.19368599552217056 No Hit CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA 713 0.188916709722719 No Hit CGGGTTATTAGTAGAAACAAGGAAATTAAAAGGTGCTCTTCAGGAGACTT 708 0.18759190811176027 No Hit GGGGGGAGCGAAAGCAGGAGAAGCAGCAGAGGGAGAGAGGGAAGCAGGCT 703 0.1862671065008015 No Hit GGGGGGAGCGAAAGCAGGAGGCAGGGAGAGGGAGAAGCAGGGTCTCTGCT 703 0.1862671065008015 No Hit GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 700 0.18547222553422626 No Hit CGGGTTATTAGTAGAAACAAGGGGCACCTCAGCGGCTCAGTGGGTTCAAG 700 0.18547222553422626 No Hit CGGGTTATTAGTAGAAACAAGGACCTCATCTGAACCAGTTTCAAAAGAAA 696 0.18441238424545925 No Hit CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC 695 0.1841474239232675 No Hit GGGGGGAGCAAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC 671 0.17778837619066543 No Hit CGGGTTATTAGTAGAAACAAGGTGGGACAGAGCAGCACCAGACTCATTTG 657 0.17407893167998092 No Hit CGGGTTATTAGTAGAAACAAGGGAAAAGGATTTCCTCAGGGTCAGTTCCC 656 0.17381397135778917 No Hit CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT 649 0.17195924910244692 No Hit CGGGTTATTAGTAGAAACAAGGGAGCATGTCACAAAGATCAGGACAGACT 648 0.17169428878025514 No Hit CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC 645 0.1708994078136799 No Hit GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT 631 0.16718996330299538 No Hit GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA 628 0.1663950823364201 No Hit CGGGTTATTAGTAGAAACAAGGACAGTTGAGCTTAATGATCGTTCAACTT 619 0.16401043943669436 No Hit CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA 618 0.1637454791145026 No Hit GGGGGGAGCGAAAGCAGGGGGAATGGCAGACAGAGGGAGAAGCAGGCTCC 603 0.15977107428162632 No Hit CGGGTTATTAGTAGAAACAAGGATAAAATTATTGTTAGAATGTAAAATTG 601 0.1592411536372428 No Hit CGGGTTATTAGTAGAAACAAGGGAAAATTCAAGAAGCTTTAGTAGCATCT 589 0.1560616297709418 No Hit GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG 575 0.1523521852602573 No Hit CGGGTTATTAGTAGAAACAAGGGGTTAATCTGAGAAAGGAGAAAAAGGTC 571 0.15129234397149027 No Hit GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA 545 0.14440337559450472 No Hit CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGGACATGCATCCAG 543 0.14387345495012122 No Hit CGGGTTATTAGTAGAAACAAGGTCGCAGGTGCCAATCAGAGCCTGCCCTG 536 0.14201873269477897 No Hit GGGGGGAGCGAAAGCAGGCAGAATGTCAGACAGAGGCAGAGAGAGAAGCA 535 0.14175377237258718 No Hit CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC 522 0.13830928818409444 No Hit CGGGTTATTAGTAGAAACAAGGCGGTAGTGGATCAGAAAGGCCTTCAGAG 521 0.13804432786190268 No Hit CGGGTTATTAGTAGAAACAAGGGCGCCTGGGTGGCTCAGTCTTAAGTGTC 503 0.13327504206245117 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGTGAGGGAGAAGCAGACTCCCTAC 501 0.13274512141806763 No Hit GGGGGGAGCGAAAGCAGGGCCTGACAGCAGAGACAGCCCAGCCAGGAGCA 483 0.1279758356186161 No Hit GGGGGGAGCGAAAGCAGGGGGAGGGGCAGAGGGAGAGGGAGAAGCAGGCT 470 0.12453135143012334 No Hit GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG 469 0.12426639110793158 No Hit CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA 461 0.12214670853039757 No Hit GGGGGGAGCGAAAGCAGGGGAACAGGGAGAGGGAGAAGCAGGCTCCCCGC 455 0.12055694659724706 No Hit GGGGGGAGCGAAAGCAGGAGAGCAGCAGTCAGAGGGAGAAGCAGGCTCCC 447 0.11843726401971305 No Hit CGGGTTATTAGTAGAAACAAGGGGAAAAGAATGTGCAGGGAGTATTAGAT 443 0.11737742273094603 No Hit GGGGGGAGCAAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC 441 0.11684750208656254 No Hit GGGGGGAGCAAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC 430 0.11393293854245327 No Hit CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA 425 0.1126081369314945 No Hit CGGGTTATTAGTAGAAACAAGGCAGCACAGTTGTGTGTTTTTCTCCAGAG 422 0.11181325596491927 No Hit CGGGTTATTAGTAGAAACAAGGACATCAGGTGCCTCAGTCGGTTAGGCAT 419 0.111018374998344 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGGGAGAGAGGCGGGCTTCCCGC 417 0.1104884543539605 No Hit CGGGTTATTAGTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCC 411 0.10889869242080999 No Hit GGGGGGAGCGAAAGCAGGGAAGAGGGCAACTGGGGTCCTTTAACCAGAGT 410 0.10863373209861822 No Hit GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC 408 0.10810381145423473 No Hit CGGGTTATTAGTAGAAACAAGGTGGAAAGCCGGGGCAACTCACTTCCAAG 405 0.10730893048765948 No Hit GGGGGGAGCGAAAGCAGGGGCAATGGGGAGAAGCAGGCTTCCCTGCTGAG 403 0.10677900984327597 No Hit CGGGTTATTAGTAGAAACAAGGGGTGGGATGTGATGGGTAAAAGGTGGTC 402 0.10651404952108422 No Hit GGGGGGAGCGAAAGCAGGGGGAATGGGAGAGAAAGAAGCAGACATCCCAC 401 0.10624908919889248 No Hit GGGGGGAGCGAAAGCAGGACCAAGAGTGGAGCCTGGCTAGATTTGCATCC 398 0.1054542082323172 No Hit GGGGGGAGCGAAAGCAGGAGAGCAGCAGTCAGAGGGAGAGGCAGGCTCCC 397 0.10518924791012546 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGAAGAAGCAGGCTTCCCGC 393 0.10412940662135844 No Hit GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA 384 0.10174476372163269 No Hit GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTACCCGC 379 0.10041996211067393 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003974404832876277 0.0 0.0 2 0.003974404832876277 0.0 0.0 3 0.003974404832876277 0.0 0.0 4 0.003974404832876277 0.0 0.0 5 0.003974404832876277 0.0 0.0 6 0.00450432547725978 0.0 0.0 7 0.004769285799451532 0.0 0.0 8 0.0052992064438350354 0.0 0.0 9 0.0052992064438350354 0.0 0.0 10-14 0.009538571598903065 0.0 0.0 15-19 0.012612111336327387 0.0 0.0 20-24 0.0152617145582449 0.0 0.0 25-29 0.018600214617860978 0.0 0.0 30-34 0.024111389319449412 0.0 0.0 35-39 0.027555873507942186 0.0 0.0 40-44 0.030417444987613108 0.0 0.0 45-49 0.03847223878224236 0.0 0.0 50-54 0.05140230250519985 0.0 0.0 55-59 0.07482479498695072 0.0 0.0 60-64 0.0971344541154962 0.0 0.0 65-69 0.1277638673608627 0.0 0.0 70-74 0.21403494826649708 0.0 0.0 75-79 0.3132890849595273 0.0 0.0 80-84 0.4019448087648875 0.0 0.0 85-89 0.5230846680709563 0.0 0.0 90-94 0.7238186081634275 0.0 0.0 95-99 1.107163202310454 0.0 0.0 100-104 1.559980392936158 0.0 0.0 105-109 1.9410993203767735 0.0 1.5897619331505108E-4 110-114 2.403773034988011 0.0 2.649603221917518E-4 115-119 3.073009816779937 0.0 3.709444510684525E-4 120-124 3.935826609965158 0.0 5.299206443835036E-4 125-129 4.6559887656823395 0.0 5.299206443835036E-4 130-134 5.512287534941644 0.0 5.299206443835036E-4 135-139 6.439913622934965 0.0 5.299206443835036E-4 140-144 7.619993905912589 0.0 5.299206443835036E-4 145-149 8.754130069022162 0.0 5.299206443835036E-4 150-154 10.036008107785857 0.0 5.299206443835036E-4 155-159 12.610150629943167 0.0 5.299206443835036E-4 160-164 14.392061788747137 0.0 5.299206443835036E-4 165-169 17.099055416451385 0.0 5.299206443835036E-4 170-174 24.065975120225747 0.0 5.299206443835036E-4 175-179 26.835128439516183 0.0 5.299206443835036E-4 180-184 28.620696050766394 0.0 5.299206443835036E-4 185-189 31.416981307049262 0.0 5.299206443835036E-4 190-194 33.74020640409098 0.0 5.299206443835036E-4 195-199 35.32477511492654 0.0 5.299206443835036E-4 200-204 37.12306082164196 0.0 6.888968376985546E-4 205-209 38.88949829762993 0.0 7.948809665752554E-4 210-214 40.59658466144695 0.0 9.538571598903065E-4 215-219 42.392379741133766 0.0 0.0010598412887670072 220-224 43.93206417339003 0.0 0.0010598412887670072 225-229 45.45177059735305 0.0 0.0010598412887670072 230-234 46.546533656584934 0.0 0.0010598412887670072 235-239 48.40491236437343 0.0 0.0010598412887670072 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGGTTA 8505 0.0 240.10287 1 GTTATTA 8550 0.0 237.69298 4 TTATTAG 8625 0.0 235.62608 5 TATTAGT 8750 0.0 233.94 6 GGTTATT 8735 0.0 232.7991 3 ATTAGTA 8890 0.0 230.94489 7 TAGTAGA 9105 0.0 227.7787 9 GGGTTAT 8965 0.0 226.82655 2 ATCGGGA 1925 0.0 221.45454 2 TTAGTAG 9365 0.0 219.75443 8 TCGGGAG 1810 0.0 206.42265 3 GCGAAAG 8120 0.0 204.87068 8 AGCGAAA 8115 0.0 204.24214 7 CGGGAGA 2060 0.0 202.77913 4 GGAGCGA 8220 0.0 201.93126 5 GGGAGCG 8195 0.0 201.50092 4 CGAAAGC 8360 0.0 200.74762 9 GATCGGG 2245 0.0 196.98218 1 GGGGAGC 13125 0.0 189.56 3 CAAAAGC 4640 0.0 181.90195 9 >>END_MODULE