FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated d3.34200000008529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated d3.34200000008529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377415
Sequences flagged as poor quality0
Sequence length251
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT98432.608004451333413No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT56521.497555741027781No Hit
GGGGGGAGCAAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT34700.9194123180053786No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG27910.7395042592371792No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG26580.7042645363856762No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC25640.6793582660996516No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA21660.5739040578673343No Hit
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC21090.5588013195024045No Hit
CGGGTTATTAGTAGAAACAAGGGCTGGAGAATAGAACCGAGTCCAGAAAT20180.5346899301829552No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG18840.49918524700926037No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC18230.4830226673555635No Hit
GGGGGGAGCGAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT16560.43877429354954095No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC15550.412013301008174No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA14710.38975663394406684No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT14670.3886967926552999No Hit
GGGGGGAGCGAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC14450.38286766556708135No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA14010.37120941139064423No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA13820.36617516526900096No Hit
CGGGTTATTAGTAGAAACAAGGTTGGTTTGATTTAGTTAAAAGCGAAGTT13540.35875627624763196No Hit
CGGGTTATTAGTAGAAACAAGGGAATAGAAACAGCTGTAAAAGGGCGCCT12210.3235165533961289No Hit
CGGGTTATTAGTAGAAACAAGGTCAACATCACCCACAAAGAGTAAATTGT11900.31530278340818463No Hit
CGGGTTATTAGTAGAAACAAGGAGGGAGGGGTGCCTGGGTGGCTCAGTCC11820.3131831008306506No Hit
CGGGTTATTAGTAGAAACAAGGTGTACTGCAGTAGAACTGAAAACGTATT11780.3121232595418836No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT11470.3039094895539393No Hit
CGGGTTATTAGTAGAAACAAGGGTTAGAAGATTTCAGTTGGATTTCTGAA11200.296755560854762No Hit
GGGGGGAGCAAAAGCAGGGGCAGTGGGAGAGGAGAAGCAGGCTTCCCTCT11030.29225123537750225No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10860.28774690990024243No Hit
CGGGTTATTAGTAGAAACAAGGGCACCTAGGTGGCTCAGTCATTATGCAT10140.2686697667024363No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG9800.2596611157479168No Hit
CGGGTTATTAGTAGAAACAAGGTAAGCTAATGAACGTAACTGTGATAAAG9380.24853278221586317No Hit
CGGGTTATTAGTAGAAACAAGGGGTCAGCATGTAAGTGGCATGAAATATG9010.23872925029476835No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA8700.23051548030682406No Hit
CGGGTTATTAGTAGAAACAAGGCTTCCAGGCTGCCAGGGTGGCTCAGTCG8400.2225666706410715No Hit
CGGGTTATTAGTAGAAACAAGGCACACCATATTGACTTCATTTCATTTTT8380.222036749996688No Hit
CGGGTTATTAGTAGAAACAAGGAGTACTTAGGGTGCCTATGTGGCTCAGT8310.22018202774134574No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC8250.2185922658081952No Hit
CGGGTTATTAGTAGAAACAAGGAATGATCTAATATAAGATATGCTCTAGA7700.20401944808764888No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC7670.20322456712107362No Hit
CGGGTTATTAGTAGAAACAAGGGAACGCTATCCAGCAGTTAACAAAAAGA7590.2011048845435396No Hit
CGGGTTATTAGTAGAAACAAGGACCCCTTTGAGCTCTGCTGCCAACAAGA7510.1989852019660056No Hit
GGGGGGAGCAAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA7430.19686551938847158No Hit
CGGGTTATTAGTAGAAACAAGGGGACAGAGAGATTTAATATATAATCTAG7370.19527575745532105No Hit
GGGGGGAGCGAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC7310.19368599552217056No Hit
CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA7130.188916709722719No Hit
CGGGTTATTAGTAGAAACAAGGAAATTAAAAGGTGCTCTTCAGGAGACTT7080.18759190811176027No Hit
GGGGGGAGCGAAAGCAGGAGAAGCAGCAGAGGGAGAGAGGGAAGCAGGCT7030.1862671065008015No Hit
GGGGGGAGCGAAAGCAGGAGGCAGGGAGAGGGAGAAGCAGGGTCTCTGCT7030.1862671065008015No Hit
GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC7000.18547222553422626No Hit
CGGGTTATTAGTAGAAACAAGGGGCACCTCAGCGGCTCAGTGGGTTCAAG7000.18547222553422626No Hit
CGGGTTATTAGTAGAAACAAGGACCTCATCTGAACCAGTTTCAAAAGAAA6960.18441238424545925No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC6950.1841474239232675No Hit
GGGGGGAGCAAAAGCAGGAGGAGTAGGAGCAGGAGAAGCAGGCTTCCTGC6710.17778837619066543No Hit
CGGGTTATTAGTAGAAACAAGGTGGGACAGAGCAGCACCAGACTCATTTG6570.17407893167998092No Hit
CGGGTTATTAGTAGAAACAAGGGAAAAGGATTTCCTCAGGGTCAGTTCCC6560.17381397135778917No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT6490.17195924910244692No Hit
CGGGTTATTAGTAGAAACAAGGGAGCATGTCACAAAGATCAGGACAGACT6480.17169428878025514No Hit
CGGGTTATTAGTAGAAACAAGGATTTGCTACTAGAATATCAAGAGACCCC6450.1708994078136799No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT6310.16718996330299538No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA6280.1663950823364201No Hit
CGGGTTATTAGTAGAAACAAGGACAGTTGAGCTTAATGATCGTTCAACTT6190.16401043943669436No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA6180.1637454791145026No Hit
GGGGGGAGCGAAAGCAGGGGGAATGGCAGACAGAGGGAGAAGCAGGCTCC6030.15977107428162632No Hit
CGGGTTATTAGTAGAAACAAGGATAAAATTATTGTTAGAATGTAAAATTG6010.1592411536372428No Hit
CGGGTTATTAGTAGAAACAAGGGAAAATTCAAGAAGCTTTAGTAGCATCT5890.1560616297709418No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5750.1523521852602573No Hit
CGGGTTATTAGTAGAAACAAGGGGTTAATCTGAGAAAGGAGAAAAAGGTC5710.15129234397149027No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA5450.14440337559450472No Hit
CGGGTTATTAGTAGAAACAAGGCTGGAGACTGTCGGGGACATGCATCCAG5430.14387345495012122No Hit
CGGGTTATTAGTAGAAACAAGGTCGCAGGTGCCAATCAGAGCCTGCCCTG5360.14201873269477897No Hit
GGGGGGAGCGAAAGCAGGCAGAATGTCAGACAGAGGCAGAGAGAGAAGCA5350.14175377237258718No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC5220.13830928818409444No Hit
CGGGTTATTAGTAGAAACAAGGCGGTAGTGGATCAGAAAGGCCTTCAGAG5210.13804432786190268No Hit
CGGGTTATTAGTAGAAACAAGGGCGCCTGGGTGGCTCAGTCTTAAGTGTC5030.13327504206245117No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGTGAGGGAGAAGCAGACTCCCTAC5010.13274512141806763No Hit
GGGGGGAGCGAAAGCAGGGCCTGACAGCAGAGACAGCCCAGCCAGGAGCA4830.1279758356186161No Hit
GGGGGGAGCGAAAGCAGGGGGAGGGGCAGAGGGAGAGGGAGAAGCAGGCT4700.12453135143012334No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG4690.12426639110793158No Hit
CGGGTTATTAGTAGAAACAAGGGATTAAAAATATTAAATGATAAGATAAA4610.12214670853039757No Hit
GGGGGGAGCGAAAGCAGGGGAACAGGGAGAGGGAGAAGCAGGCTCCCCGC4550.12055694659724706No Hit
GGGGGGAGCGAAAGCAGGAGAGCAGCAGTCAGAGGGAGAAGCAGGCTCCC4470.11843726401971305No Hit
CGGGTTATTAGTAGAAACAAGGGGAAAAGAATGTGCAGGGAGTATTAGAT4430.11737742273094603No Hit
GGGGGGAGCAAAAGCAGGGCGAGCAGCAGAGACTCCCTGAACAGGGAGCC4410.11684750208656254No Hit
GGGGGGAGCAAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTTCCTGC4300.11393293854245327No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA4250.1126081369314945No Hit
CGGGTTATTAGTAGAAACAAGGCAGCACAGTTGTGTGTTTTTCTCCAGAG4220.11181325596491927No Hit
CGGGTTATTAGTAGAAACAAGGACATCAGGTGCCTCAGTCGGTTAGGCAT4190.111018374998344No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGGGAGAGAGGCGGGCTTCCCGC4170.1104884543539605No Hit
CGGGTTATTAGTAGAAACAAGGATTAGCACACAGTCTAGTTAATAAAGCC4110.10889869242080999No Hit
GGGGGGAGCGAAAGCAGGGAAGAGGGCAACTGGGGTCCTTTAACCAGAGT4100.10863373209861822No Hit
GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC4080.10810381145423473No Hit
CGGGTTATTAGTAGAAACAAGGTGGAAAGCCGGGGCAACTCACTTCCAAG4050.10730893048765948No Hit
GGGGGGAGCGAAAGCAGGGGCAATGGGGAGAAGCAGGCTTCCCTGCTGAG4030.10677900984327597No Hit
CGGGTTATTAGTAGAAACAAGGGGTGGGATGTGATGGGTAAAAGGTGGTC4020.10651404952108422No Hit
GGGGGGAGCGAAAGCAGGGGGAATGGGAGAGAAAGAAGCAGACATCCCAC4010.10624908919889248No Hit
GGGGGGAGCGAAAGCAGGACCAAGAGTGGAGCCTGGCTAGATTTGCATCC3980.1054542082323172No Hit
GGGGGGAGCGAAAGCAGGAGAGCAGCAGTCAGAGGGAGAGGCAGGCTCCC3970.10518924791012546No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGAAGAAGCAGGCTTCCCGC3930.10412940662135844No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA3840.10174476372163269No Hit
GGGGGGAGCGAAAGCAGGGGGAGTGGGAGAGGGAGAAGCAGGCTACCCGC3790.10041996211067393No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA85050.0240.102871
GTTATTA85500.0237.692984
TTATTAG86250.0235.626085
TATTAGT87500.0233.946
GGTTATT87350.0232.79913
ATTAGTA88900.0230.944897
TAGTAGA91050.0227.77879
GGGTTAT89650.0226.826552
ATCGGGA19250.0221.454542
TTAGTAG93650.0219.754438
TCGGGAG18100.0206.422653
GCGAAAG81200.0204.870688
AGCGAAA81150.0204.242147
CGGGAGA20600.0202.779134
GGAGCGA82200.0201.931265
GGGAGCG81950.0201.500924
CGAAAGC83600.0200.747629
GATCGGG22450.0196.982181
GGGGAGC131250.0189.563
CAAAAGC46400.0181.901959
Produced by FastQC (version 0.11.2)