FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated c6.34200000008692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated c6.34200000008692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418759
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA93722.23804145104941No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC85442.0403143574227656No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG79751.9044366807638762No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG75671.8070059389768338No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG54251.2954945445948625No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC51041.2188394756888807No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG36030.8603994182811593No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA34760.8300717118915653No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC27190.6492994777425679No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG23840.5693011971085994No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA23640.5645251803543327No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT23390.5585551594114991No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA22500.5373018848550121No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG19440.46422882851473046No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT14610.3488880238991879No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA13620.32524674096556727No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13540.3233363342638606No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA12110.2891878144708532No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA9850.2352188251476386No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA7810.18650345425411752No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA7100.16954859477647047No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT5770.13778808336059645No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACATTAATTG4360.10411716524301567No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC30150.0236.873969
TTATTAG44500.0232.337085
TATTAGT45000.0230.299996
GGGTTAT45050.0228.68482
ATTAGTA45700.0227.576587
CGGGTTA45550.0227.250271
GAGCGGC11750.0225.191489
GTTATTA45950.0224.738854
GGTTATT46100.0223.476153
AAGCGGC3400.0219.779439
TAGTAGA47800.0219.11619
ACGGGAG7550.0214.17223
ATCCGGA2650.0212.641512
CGAAAGC28500.0211.043879
GCGAAAG28700.0209.146358
GGAGCGA28850.0207.634315
TTAGTAG50350.0206.80248
TCCGGAG2500.0205.799993
GAGCGAA29200.0205.565056
AGCGAAA29350.0204.09717
Produced by FastQC (version 0.11.2)