FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated c5.3420000000861b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated c5.3420000000861b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241833
Sequences flagged as poor quality0
Sequence length251
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG116654.823576600381255No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA83733.462306633089777No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT63482.6249519296373944No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT57562.3801549002824265No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA28101.1619588724450343No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA27881.1528616855433296No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG22910.9473479632639052No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT20400.843557330885363No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA18960.7840121075287492No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA12330.5098559749910062No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG11340.46891863393333416No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10460.4325298863265146No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC9780.4044113086303358No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA7320.3026882187294538No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG5960.24645106333709624No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA3060.12653359963280447No Hit
GGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAA2980.12322553166854812No Hit
GGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACT2890.11950395520875975No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC2880.11909044671322773No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACATTAATTG2530.1046176493696063No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA2490.10296361538747814No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGGGAG18650.0237.760543
GAGCGGC12500.0235.185919
TTATTAG24350.0232.912125
GAGGGGC30850.0231.882399
TATTAGT24650.0231.071376
ATCGGGA18500.0229.75652
TCGGGAG15200.0228.061983
ATTAGTA24950.0227.801977
TTAGTAG25450.0223.326498
GTTATTA25600.0220.582474
AGAGCGG14200.0219.969228
AAGGGGC13150.0219.834729
GGGTTAT25750.0219.773222
TAGTAGA26000.0219.073439
AAGCGGC4500.0215.042669
CGGGTTA26700.0212.412381
GGTTATT26600.0212.289923
TGGGAGA22800.0212.213174
CGGGAGA18450.0211.125564
GGGGAGC36600.0210.848023
Produced by FastQC (version 0.11.2)