FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated c4.34200000008593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated c4.34200000008593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16232
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGGGGAGGGGGGAGAAAT210.12937407589945785No Hit
GAACGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGGGGAGGGGGGAGAAAT210.12937407589945785No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAGAGGGGAGGGAAGGGGGGAGAAAT180.11089206505667816No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGAG100.0014628336245.000023
TTGGAAG100.0014628336245.000023
ATTCGGA100.0014628336245.000022
ATTGGCA100.0014628336245.000022
GATTGGC100.0014628336245.000021
TCGAGAG100.0014628336245.000025
AAGCGGT151.4919586E-5245.09
GGTCTGA151.4919586E-5245.01
CTGGAGA151.4919586E-5245.04
GAGGGGC5700.0242.850889
GAGCGGC2650.0240.377359
ATCGGGA2400.0239.895832
TTGGGAG1500.0236.833333
CGGGAGA4400.0236.647744
TCGGGAG2650.0235.754713
GAGAGCG2550.0235.392177
ATTGGGA1250.0235.200012
AAGGGGC1200.0234.791679
AGAGCGG2800.0231.8758
ACGGGAG1850.0231.756763
Produced by FastQC (version 0.11.2)