FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated c2.34200000008494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated c2.34200000008494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239242
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC21700.9070313740898337No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG20450.8547830230477926No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA19910.8322117353976308No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG19700.823434012422568No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG13590.568044072529071No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG12490.5220655236120748No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT12300.5141237742536845No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA11340.47399704065339704No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG11240.4698171725700337No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG10070.42091271599468316No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT8120.3394052883690991No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA8070.33731535432741744No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA7570.316416013910601No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA6690.27963317477700406No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC6610.2762892803103134No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC5480.22905677096830826No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT5250.21944307437657268No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA5240.21902508756823633No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA4910.2052315228931375No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA4760.19896172076809257No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG4700.19645379991807457No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC4570.1910199714097023No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4380.18307822205131205No Hit
CGGGTTATTAGTAGAAACAAGGCCCGTGAGTCAGTAGTGAAACCAGAAGT4360.1822422484346394No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT3970.16594076290952256No Hit
GGGGGGAGCGAAAGCAGGAGAAAACAAGTCCTAGTAAGGATGTGGACGTT3940.1646868024845136No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC3870.1617608948261593No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC3870.1617608948261593No Hit
GGGGGGAGCGAAAGCAGGGTGAACAACAGGCAGAGGGAGAGGGAGAAACA3740.156327066317787No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA3670.1534011586594327No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA3530.1475493433427241No Hit
GGGGGGAGCGAAAGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACAT3460.1446234356843698No Hit
CGGGTTATTAGTAGAAACAAGGATGAAATACACTAGGCGAGCTTGCCAGA3180.1329198050509526No Hit
GGGGGGAGCAAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA3050.12748597654258031No Hit
CGGGTTATTAGTAGAAACAAGGTCAAAGAGCAGGTTCTGGGACAGTAGGA2970.12414208207588968No Hit
GGGGGGAGCAAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA2890.12079818760919905No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC2860.11954422718419007No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA2690.11243845144247248No Hit
GGGGGGAGCGAAAGCAGGGCAGAGCCAGCTCCAGTCACTGCTCTCTGGTG2600.10867657016744553No Hit
CGGGTTATTAGTAGAAACAAGGCAGTGGGGAACGGGAGTCATAAGCCCTT2540.10616864931742753No Hit
GGGGGGAGCGAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCT2500.10449670208408222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA20700.0239.673921
ATTAGTA21800.0229.827997
GGGTTAT21650.0229.722852
TATTAGT21950.0227.699316
GTTATTA21850.0227.620154
TTATTAG22100.0225.045265
TAGTAGA22800.0219.210539
TTAGTAG23150.0215.896338
GGTTATT23900.0207.58373
GGGGGGA75300.0202.377151
GGAGCGA52000.0195.764435
GAGCGAA52200.0195.249056
GGGGAGC77250.0194.572813
AGCGAAA52650.0194.278247
GCGAAAG53650.0192.027028
GGGAGCG53000.0191.608494
CGAAAGC54250.0191.258079
GGGGGAG83500.0180.889242
GAGCAAA27600.0178.423926
GGAGCAA28050.0176.434945
Produced by FastQC (version 0.11.2)