FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated b5.3420000000860e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated b5.3420000000860e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375525
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT157854.203448505425738No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG116423.1001930630450705No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT114273.042939884162173No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA66361.767126023567006No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA65531.7450236335796552No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA63711.6965581519206445No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG48721.2973836628719793No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA47751.2715531589108582No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT42511.1320151787497503No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC40391.075560881432661No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG27950.7442913254776645No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC27240.7253844617535451No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG23000.6124758671193662No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA19780.526729245722655No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC15790.42047799747020836No Hit
GGGGGGAGCAAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA14570.387990147127355No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG12680.3376606084814593No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA10100.26895679382198256No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8190.21809466746554826No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG6520.17362359363557686No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA6000.15977631316157379No Hit
GGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTA4930.13128287064775981No Hit
GGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACT3950.10518607283136942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT56800.0240.898882
TTATTAG57300.0240.079515
CGGGTTA57000.0239.83871
GGTTATT57250.0239.219333
TATTAGT58300.0237.22226
GTTATTA57900.0236.745364
ATTAGTA58600.0236.634897
TAGTAGA61100.0228.155559
GGGGGGA62000.0220.694461
TTAGTAG63700.0218.458478
GGGGGAG62900.0213.83642
GGAGCGA46550.0207.628655
GAGCGAA46650.0206.658396
AGCGAAA47400.0204.680657
GCGAAAG47950.0203.865748
GGGGAGC66150.0203.145283
CGAAAGC48750.0201.776649
GGGAGCG49200.0194.204574
GAGGGGC6450.0180.42389
GAGCGGC3600.0176.941969
Produced by FastQC (version 0.11.2)