FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated b4.34200000008586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated b4.34200000008586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71099
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG14552.0464422847016133No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA11921.6765355349582975No Hit
GGGGGGAGCGAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC6820.9592258681556702No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG5630.7918536125683906No Hit
GGGGGGAGCAAAAGCAGGAGGAGTGGGAGAGAGGGAGGCACGCTTCCTGC5070.7130901981743766No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT4210.5921320974978551No Hit
CGGGTTATTAGTAGAAACAAGGATCAAACATTTTAGATGTATTGAATTGT4160.5850996497841038No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC4120.5794736916131029No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG4120.5794736916131029No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC3930.552750390300848No Hit
GGGGGGAGCGAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA3350.47117399682133365No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT2940.41350792556857335No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC2800.3938170719700699No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA2530.35584185431581317No Hit
GGGGGGAGCGAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC2240.3150536575760559No Hit
GGGGGGAGCAAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT2190.3080212098623047No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG1890.2658265235797972No Hit
CGGGTTATTAGTAGAAACAAGGTGAAGGATGTCATCGGTTTCCCTTGCCC1840.25879407586604597No Hit
CGGGTTATTAGTAGAAACAAGGTAGGGGGGAAACAGGCTGATGACATATT1840.25879407586604597No Hit
CGGGTTATTAGTAGAAACAAGGTAGTGGCACCTGGGTGGCTCAGTGGCCT1670.2348837536392917No Hit
GGGGGGAGCAAAAGCAGGGAAAAAAATAAGTCGATAGAAACAAACCCAAA1490.2095669418697872No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC1350.18987608827128372No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC1280.18003066147203195No Hit
GGGGGGAGCGAAAGCAGGGAGCTGAGTTAGGAGGCAATTACAATAACCCA1170.1645592765017792No Hit
CGGGTTATTAGTAGAAACAAGGCTAATTTAAAGGTAAGATTTAGGGGCGC1130.15893331833077823No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA1100.15471384970252747No Hit
GGGGGGAGCGAAAGCAGGGGTAGTGGCAGACAAAGGGAGCAGTAGGCTCC1040.14627491244602595No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG1010.1420554438177752No Hit
GGGGGGAGCGAAAGCAGGATGAGAGACAGATTTCTGTTCCTTACGCCTTT970.13642948564677423No Hit
GGGGGGAGCGAAAGCAGGGGGAGCAGCAGAGGGAGAGGAGGAAGCAGGCT940.13221001701852347No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT940.13221001701852347No Hit
CGGGTTATTAGTAGAAACAAGGTTTAGTAGTAAATATTATAAAAGTGTTG930.1308035274757732No Hit
GGGGGGAGCGAAAGCAGGAACAGCAGCACTGGGTTCCTCTGCTGTCACTT920.12939703793302298No Hit
GGGGGGAGCGAAAGCAGGGGATGGGGGGCCCGTGGGAGGGAGGCCTGAAG900.12658405884752247No Hit
GGGGGGAGCGAAAGCAGGCAGGGAAGCAGACAGAGAGGAGGAAGCAGGGT890.12517756930477222No Hit
CGGGTTATTAGTAGAAACAAGGGGAAGAAGAGACTGTTCCGGGAATAGAA870.12236459021927172No Hit
GGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCA860.12095810067652146No Hit
CGGGTTATTAGTAGAAACAAGGTATTGGTATGAAAGAACTATGCTAGGAT860.12095810067652146No Hit
GGGGGGAGCAAAAGCAGGGAGAGCAGCAGGCAGGAGGAGAAGCAGGGTCC810.11392565296277023No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC800.11251916342001997No Hit
GGGGGGAGCGAAAGCAGGTTAAGAGTAAATTACTAAGGAAAAAACTACTC770.10829969479176922No Hit
GGGGGGAGCAAAAGCAGGTTGCAAATCCTATAGAGAAAAGAAATAGCTTA760.10689320524901898No Hit
GGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGT740.10408022616351849No Hit
GGGGGGAGCGAAAGCAGGTATCAACTGAAGTGTTGAAGGATATACAATAA720.10126724707801796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGG100.0014732967245.000021
TATTAGT9000.0239.555566
TTATTAG9150.0235.628425
GTTATTA9250.0233.081074
TAGTAGA9450.0228.148159
ATTAGTA9550.0225.759167
GGTTATT9600.0224.583333
CGGGTTA9550.0224.476441
GGGTTAT9550.0224.476442
TCGGGAG2750.0222.727283
ATCGGGA3050.0220.901642
CGGGAGA4000.0220.54
CGAAAGC12050.0211.452299
GAGCGAA11850.0210.886086
AGCGAAA11950.0210.146457
GGAGCGA11900.0210.05
GCGAAAG12100.0208.553718
GAGAGCG2000.0208.257
GGGAGCG12050.0206.36934
TTAGTAG10600.0203.396248
Produced by FastQC (version 0.11.2)