FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated b3.3420000000850f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated b3.3420000000850f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences388218
Sequences flagged as poor quality0
Sequence length251
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC55221.4223966946406401No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG55041.4177601244661504No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA51031.314467644467799No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG44641.1498694032734185No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG33350.8590534184401548No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA24730.6370132245284866No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG23560.6068755183943042No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG22200.5718436548537162No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA16100.4147154433849023No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC15650.4031240179486783No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG15090.38869913296137737No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC13650.35160657156546066No Hit
GGGGGGAGCGAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT12140.3127108995461313No Hit
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT11630.2995739507184108No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA10600.2730424658310537No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT9020.23234368318831175No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8410.21663086204143034No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGAAGTGGATGATGGC7550.1944783600966467No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG7430.19138731331365366No Hit
GGGGGGAGCGAAAGCAGGTAACCGGACTGCTGTAGAACCCAAGGCCCTCC6460.1664013518177931No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA6280.1617647816433035No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA5900.15197646683049215No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA5680.1463095477283382No Hit
GGGGGGAGCAAAAGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT5230.13471812229211422No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT4950.12750567979846375No Hit
GGGGGGAGCGAAAGCAGGTGCATTGACTGAAGATCCAGATGAAGGCACAT4920.12673291810271547No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC4870.12544498194313503No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA4380.11282320757924671No Hit
GGGGGGAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG4220.10870181186858931No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA4170.10741387570900886No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC4040.10406524169409971No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT4000.10303489276643535No Hit
CGGGTTATTAGTAGAAACAAGGCTCCTCAAGAAATCATCTCATACATTCT3960.102004543838771No Hit
CGGGTTATTAGTAGAAACAAGGTCACTTGCAGAGCATGGTGAGACCTGCT3890.10020143321535838No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAT36950.0232.065112
TATTAGT37750.0229.094156
TTATTAG37850.0227.194345
CGGGTTA38150.0225.728841
ATTAGTA38750.0224.762667
TAGTAGA39100.0224.317189
ATCCGGA2350.0224.143882
GTTATTA39500.0217.393834
GGTTATT39500.0217.083713
TTAGTAG41000.0213.025678
ATCGGGA17350.0204.750412
GGAGCGA54100.0203.10545
GCGAAAG54500.0202.513788
AGCGAAA54400.0201.534977
GAGCGAA54600.0201.469796
GGGAGCG55150.0197.905784
CGAAAGC56500.0195.995579
GGGGGGA85000.0191.383931
GGGGAGC83550.0191.186583
GATCGGG19350.0189.285131
Produced by FastQC (version 0.11.2)