FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated b2.34200000008487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated b2.34200000008487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607802
Sequences flagged as poor quality0
Sequence length251
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGCGAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT43810.7207939427642555No Hit
CGGGTTATTAGTAGAAACAAGGATTCCTTCAACTCAGTAATTGAGTTATC33530.5516599155646082No Hit
CGGGTTATTAGTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCC30450.5009855183102392No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG29710.4888105007880856No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC28580.4702189199772294No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG26060.4287580494963821No Hit
CGGGTTATTAGTAGAAACAAGGATGTCATCTTCCTTCACTTGTAGGGTCC25860.4254675042201243No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA25060.41230532311509344No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG24760.40736950520070686No Hit
GGGGGGAGCGAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA21590.3552143625720218No Hit
GGGGGGAGCGAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG19730.32461229150282495No Hit
CGGGTTATTAGTAGAAACAAGGTTAGGGGCTTCTTCTCAGACTTTCAGTA16980.27936729395428117No Hit
GGGGGGAGCGAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA16970.2792027666904683No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG16180.26620511284925025No Hit
GGGGGGAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCT15470.2545236771185353No Hit
GGGGGGAGCAAAAGCAGGGAAACATCTGGATGTATAAAAAAATGCTCATG14240.23428682366955028No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC11790.1939776440353931No Hit
GGGGGGAGCGAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG11750.19331953498014157No Hit
GGGGGGAGCAAAAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCA11000.18097999019417507No Hit
GGGGGGAGCGAAAGCAGGCTGGAAACTTCAGAGCAGTTGCTCGGATTACC10150.16699517277007972No Hit
GGGGGGAGCGAAAGCAGGGTGAGTGAGGGAGATGCGTGGGTTGGGTAACA9420.15498468251173902No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC8690.1429741922533983No Hit
GGGGGGAGCAAAAGCAGGACAATGGGAAGAGCTAGGGCCACAGCTGGGAG8220.13524141085419264No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG8030.1321153928417478No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC7590.12487619323398079No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT6930.11401739382233031No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG6690.11006873949082102No Hit
GGGGGGAGCGAAAGCAGGGCAGAGCCAGCTCCAGTCACTGCTCTCTGGTG6620.10891704864413082No Hit
GGGGGGAGCAAAAGCAGGGTTTCAAAAGGTTATTTGTACACCTGTGTTCA6420.10562650336787309No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTA45200.0236.056431
GGGTTAT45950.0229.537552
TATTAGT46500.0227.612926
TTATTAG47350.0222.750785
ATTAGTA47850.0222.727267
GTTATTA48050.0217.976064
TAGTAGA49700.0216.161979
TTAGTAG49950.0213.853878
GGGGGGA144650.0205.281721
GGTTATT52300.0200.965593
GGAGCGA101600.0192.551675
AGCGAAA102450.0192.269417
GAGCGAA102100.0191.608726
GCGAAAG105900.0188.319188
GGGAGCG103500.0187.951694
CGAAAGC108300.0185.616339
GGGGAGC158000.0185.300633
GGGGGAG160400.0183.21542
GAGCAAA55250.0178.705896
GGAGCAA56150.0176.714165
Produced by FastQC (version 0.11.2)