##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	000000000-AE52Y l01n02 flu mrt-pcr updated a8.34200000008777.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	23
Sequences flagged as poor quality	0
Sequence length	251
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.956521739130434	0.0	0.0	0.0	0.0	0.0
2	25.130434782608695	0.0	0.0	0.0	0.0	0.0
3	20.043478260869566	0.0	0.0	0.0	0.0	0.0
4	20.695652173913043	0.0	0.0	0.0	0.0	0.0
5	24.608695652173914	0.0	0.0	0.0	0.0	0.0
6	27.82608695652174	0.0	0.0	0.0	0.0	0.0
7	20.304347826086957	0.0	0.0	0.0	0.0	0.0
8	25.652173913043477	0.0	0.0	0.0	0.0	0.0
9	20.217391304347824	0.0	0.0	0.0	0.0	0.0
10-14	22.66086956521739	0.0	0.0	0.0	0.0	0.0
15-19	23.486956521739128	0.0	0.0	0.0	0.0	0.0
20-24	24.85217391304348	0.0	0.0	0.0	0.0	0.0
25-29	22.269565217391303	0.0	0.0	0.0	0.0	0.0
30-34	21.11304347826087	0.0	0.0	0.0	0.0	0.0
35-39	22.29565217391304	0.0	0.0	0.0	0.0	0.0
40-44	21.391304347826086	0.0	0.0	0.0	0.0	0.0
45-49	22.034782608695654	0.0	0.0	0.0	0.0	0.0
50-54	22.95652173913043	0.0	0.0	0.0	0.0	0.0
55-59	22.104347826086958	0.0	0.0	0.0	0.0	0.0
60-64	20.391304347826086	0.0	0.0	0.0	0.0	0.0
65-69	21.70434782608696	0.0	0.0	0.0	0.0	0.0
70-74	25.37391304347826	0.0	0.0	0.0	0.0	0.0
75-79	29.930434782608693	0.0	0.0	0.0	0.0	0.0
80-84	22.63478260869565	0.0	0.0	0.0	0.0	0.0
85-89	20.539130434782606	0.0	0.0	0.0	0.0	0.0
90-94	21.295652173913048	0.0	0.0	0.0	0.0	0.0
95-99	20.63478260869565	0.0	0.0	0.0	0.0	0.0
100-104	20.8	0.0	0.0	0.0	0.0	0.0
105-109	20.026086956521738	0.0	0.0	0.0	0.0	0.0
110-114	20.34782608695652	0.0	0.0	0.0	0.0	0.0
115-119	21.008695652173913	0.0	0.0	0.0	0.0	0.0
120-124	21.356521739130436	0.0	0.0	0.0	0.0	0.0
125-129	20.30434782608696	0.0	0.0	0.0	0.0	0.0
130-134	20.121739130434783	0.0	0.0	0.0	0.0	0.0
135-139	20.469565217391306	0.0	0.0	0.0	0.0	0.0
140-144	20.713043478260868	0.0	0.0	0.0	0.0	0.0
145-149	20.078260869565216	0.0	0.0	0.0	0.0	0.0
150-154	19.643478260869564	0.0	0.0	0.0	0.0	0.0
155-159	19.869565217391305	0.0	0.0	0.0	0.0	0.0
160-164	19.37391304347826	0.0	0.0	0.0	0.0	0.0
165-169	19.51304347826087	0.0	0.0	0.0	0.0	0.0
170-174	20.391304347826086	0.0	0.0	0.0	0.0	0.0
175-179	19.9304347826087	0.0	0.0	0.0	0.0	0.0
180-184	19.304347826086957	0.0	0.0	0.0	0.0	0.0
185-189	19.27826086956522	0.0	0.0	0.0	0.0	0.0
190-194	19.286956521739132	0.0	0.0	0.0	0.0	0.0
195-199	18.652173913043477	0.0	0.0	0.0	0.0	0.0
200-204	19.086956521739133	0.0	0.0	0.0	0.0	0.0
205-209	18.37391304347826	0.0	0.0	0.0	0.0	0.0
210-214	17.982608695652175	0.0	0.0	0.0	0.0	0.0
215-219	18.130434782608695	0.0	0.0	0.0	0.0	0.0
220-224	17.826086956521742	0.0	0.0	0.0	0.0	0.0
225-229	17.582608695652173	0.0	0.0	0.0	0.0	0.0
230-234	17.76521739130435	0.0	0.0	0.0	0.0	0.0
235-239	16.904347826086955	0.0	0.0	0.0	0.0	0.0
240-244	17.991304347826087	0.0	0.0	0.0	0.0	0.0
245-249	16.860869565217392	0.0	0.0	0.0	0.0	0.0
250-251	17.52173913043478	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1110	1	0.0
1110	2	-6.5
1110	3	0.0
1110	4	0.0
1110	5	0.0
1110	6	0.0
1110	7	0.0
1110	8	-8.5
1110	9	0.0
1110	10-14	1.3000000000000007
1110	15-19	2.200000000000003
1110	20-24	0.09999999999999787
1110	25-29	3.8000000000000007
1110	30-34	3.5
1110	35-39	-3.3999999999999986
1110	40-44	2.1000000000000014
1110	45-49	1.1999999999999993
1110	50-54	-3.599999999999998
1110	55-59	-7.199999999999999
1110	60-64	1.200000000000001
1110	65-69	-0.40000000000000036
1110	70-74	4.199999999999999
1110	75-79	-3.0
1110	80-84	0.40000000000000036
1110	85-89	0.40000000000000036
1110	90-94	1.0
1110	95-99	-0.3999999999999986
1110	100-104	1.0
1110	105-109	1.4000000000000021
1110	110-114	0.1999999999999993
1110	115-119	3.6000000000000014
1110	120-124	-0.10000000000000142
1110	125-129	4.800000000000001
1110	130-134	2.400000000000002
1110	135-139	0.20000000000000284
1110	140-144	2.3000000000000007
1110	145-149	-1.0
1110	150-154	-2.1000000000000014
1110	155-159	2.6000000000000014
1110	160-164	0.3999999999999986
1110	165-169	1.8000000000000007
1110	170-174	-3.0
1110	175-179	-3.0999999999999996
1110	180-184	0.5999999999999996
1110	185-189	-1.0999999999999996
1110	190-194	-2.0
1110	195-199	-1.799999999999999
1110	200-204	3.900000000000002
1110	205-209	-3.3000000000000007
1110	210-214	0.0
1110	215-219	-1.4000000000000004
1110	220-224	-0.40000000000000213
1110	225-229	2.6000000000000014
1110	230-234	0.0
1110	235-239	0.7999999999999989
1110	240-244	1.0
1110	245-249	-1.0
1110	250-251	2.5
2108	1	0.0
2108	2	6.5
2108	3	0.0
2108	4	0.0
2108	5	0.0
2108	6	0.0
2108	7	0.0
2108	8	8.5
2108	9	0.0
2108	10-14	-1.3000000000000007
2108	15-19	-2.1999999999999993
2108	20-24	-0.10000000000000142
2108	25-29	-3.799999999999999
2108	30-34	-3.5
2108	35-39	3.400000000000002
2108	40-44	-2.0999999999999996
2108	45-49	-1.200000000000001
2108	50-54	3.6000000000000014
2108	55-59	7.199999999999999
2108	60-64	-1.1999999999999993
2108	65-69	0.3999999999999986
2108	70-74	-4.200000000000003
2108	75-79	3.0
2108	80-84	-0.3999999999999986
2108	85-89	-0.40000000000000036
2108	90-94	-1.0
2108	95-99	0.40000000000000213
2108	100-104	-1.0
2108	105-109	-1.3999999999999986
2108	110-114	-0.1999999999999993
2108	115-119	-3.599999999999998
2108	120-124	0.09999999999999787
2108	125-129	-4.800000000000001
2108	130-134	-2.3999999999999986
2108	135-139	-0.1999999999999993
2108	140-144	-2.3000000000000007
2108	145-149	1.0
2108	150-154	2.099999999999998
2108	155-159	-2.5999999999999996
2108	160-164	-0.40000000000000036
2108	165-169	-1.799999999999999
2108	170-174	3.0
2108	175-179	3.1000000000000014
2108	180-184	-0.5999999999999996
2108	185-189	1.1000000000000014
2108	190-194	2.0
2108	195-199	1.8000000000000007
2108	200-204	-3.8999999999999986
2108	205-209	3.3000000000000007
2108	210-214	0.0
2108	215-219	1.4000000000000004
2108	220-224	0.3999999999999986
2108	225-229	-2.5999999999999996
2108	230-234	0.0
2108	235-239	-0.8000000000000007
2108	240-244	-1.0
2108	245-249	1.0
2108	250-251	-2.5
>>END_MODULE
>>Per sequence quality scores	fail
#Quality	Count
15	1.0
16	8.0
17	5.0
18	2.0
19	2.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	1.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	2.0
33	0.0
34	0.0
35	0.0
36	1.0
37	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	73.91304347826086	4.3478260869565215	4.3478260869565215	17.391304347826086
2	17.391304347826086	73.91304347826086	0.0	8.695652173913043
3	21.73913043478261	8.695652173913043	65.21739130434783	4.3478260869565215
4	21.73913043478261	13.043478260869565	17.391304347826086	47.82608695652174
5	69.56521739130434	0.0	21.73913043478261	8.695652173913043
6	69.56521739130434	0.0	26.08695652173913	4.3478260869565215
7	60.86956521739131	26.08695652173913	0.0	13.043478260869565
8	8.695652173913043	73.91304347826086	13.043478260869565	4.3478260869565215
9	56.52173913043478	17.391304347826086	21.73913043478261	4.3478260869565215
10-14	52.17391304347826	28.695652173913043	9.565217391304348	9.565217391304348
15-19	50.43478260869565	13.043478260869565	16.52173913043478	20.0
20-24	72.17391304347827	15.65217391304348	6.086956521739131	6.086956521739131
25-29	26.956521739130434	56.52173913043478	13.043478260869565	3.4782608695652173
30-34	55.65217391304348	27.82608695652174	11.304347826086957	5.217391304347826
35-39	40.869565217391305	27.82608695652174	20.869565217391305	10.434782608695652
40-44	41.73913043478261	36.52173913043478	15.65217391304348	6.086956521739131
45-49	27.82608695652174	45.21739130434783	19.130434782608695	7.82608695652174
50-54	40.869565217391305	10.434782608695652	30.434782608695656	18.26086956521739
55-59	61.73913043478261	9.565217391304348	14.782608695652174	13.91304347826087
60-64	40.0	12.173913043478262	19.130434782608695	28.695652173913043
65-69	10.434782608695652	22.608695652173914	40.0	26.956521739130434
70-74	8.695652173913043	68.69565217391305	16.52173913043478	6.086956521739131
75-79	5.217391304347826	80.8695652173913	7.82608695652174	6.086956521739131
80-84	11.304347826086957	66.95652173913044	10.434782608695652	11.304347826086957
85-89	9.565217391304348	50.43478260869565	20.0	20.0
90-94	21.73913043478261	28.695652173913043	27.82608695652174	21.73913043478261
95-99	20.0	30.434782608695656	18.26086956521739	31.30434782608696
100-104	18.26086956521739	34.78260869565217	20.0	26.956521739130434
105-109	22.608695652173914	40.0	13.043478260869565	24.347826086956523
110-114	18.26086956521739	27.82608695652174	27.82608695652174	26.08695652173913
115-119	26.956521739130434	28.695652173913043	25.217391304347824	19.130434782608695
120-124	15.65217391304348	32.17391304347826	22.608695652173914	29.565217391304348
125-129	17.391304347826086	40.869565217391305	18.26086956521739	23.47826086956522
130-134	16.52173913043478	33.04347826086956	25.217391304347824	25.217391304347824
135-139	10.434782608695652	33.91304347826087	24.347826086956523	31.30434782608696
140-144	13.91304347826087	32.17391304347826	25.217391304347824	28.695652173913043
145-149	17.391304347826086	30.434782608695656	24.347826086956523	27.82608695652174
150-154	13.043478260869565	30.434782608695656	23.47826086956522	33.04347826086956
155-159	16.52173913043478	31.30434782608696	25.217391304347824	26.956521739130434
160-164	18.26086956521739	30.434782608695656	28.695652173913043	22.608695652173914
165-169	16.52173913043478	27.82608695652174	31.30434782608696	24.347826086956523
170-174	14.782608695652174	30.434782608695656	30.434782608695656	24.347826086956523
175-179	17.391304347826086	26.956521739130434	31.30434782608696	24.347826086956523
180-184	13.043478260869565	26.08695652173913	34.78260869565217	26.08695652173913
185-189	19.130434782608695	24.347826086956523	34.78260869565217	21.73913043478261
190-194	20.869565217391305	24.347826086956523	27.82608695652174	26.956521739130434
195-199	17.391304347826086	30.434782608695656	26.956521739130434	25.217391304347824
200-204	15.65217391304348	26.956521739130434	29.565217391304348	27.82608695652174
205-209	20.0	26.08695652173913	24.347826086956523	29.565217391304348
210-214	16.52173913043478	22.608695652173914	33.91304347826087	26.956521739130434
215-219	22.608695652173914	28.695652173913043	24.347826086956523	24.347826086956523
220-224	16.52173913043478	29.565217391304348	25.217391304347824	28.695652173913043
225-229	15.65217391304348	33.04347826086956	21.73913043478261	29.565217391304348
230-234	20.0	33.04347826086956	26.08695652173913	20.869565217391305
235-239	17.391304347826086	28.695652173913043	32.17391304347826	21.73913043478261
240-244	17.391304347826086	26.956521739130434	35.65217391304348	20.0
245-249	9.565217391304348	35.65217391304348	34.78260869565217	20.0
250-251	17.391304347826086	32.608695652173914	19.565217391304348	30.434782608695656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.3333333333333333
36	0.3333333333333333
37	0.0
38	0.6666666666666666
39	0.6666666666666666
40	0.6666666666666666
41	0.6666666666666666
42	0.3333333333333333
43	0.3333333333333333
44	1.0
45	1.3333333333333333
46	0.6666666666666666
47	0.3333333333333333
48	0.6666666666666666
49	1.0
50	1.0
51	1.0
52	0.0
53	0.0
54	0.3333333333333333
55	0.0
56	0.0
57	0.3333333333333333
58	0.3333333333333333
59	0.0
60	0.0
61	0.0
62	0.0
63	0.0
64	0.0
65	0.0
66	0.0
67	0.0
68	0.0
69	0.0
70	0.0
71	0.0
72	0.0
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-251	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
251	23.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	100.0
#Duplication Level	Percentage of deduplicated	Percentage of total
1	100.0	100.0
2	0.0	0.0
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCT	1	4.3478260869565215	No Hit
GATCGGGAGAGGGGCGGGTAGGGGAAAAGGGGAGGGGATAGAGGAGAGTT	1	4.3478260869565215	No Hit
GATCGGGAGAAAGGCGGGGGGGGGAAGAGGGGAGGGTATTATGGAAAGCA	1	4.3478260869565215	No Hit
GATCGGGAGAGCGGCGTGGAGGGGAAGAGGGGGGGCGAAAGTGTAGATCT	1	4.3478260869565215	No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGAAGGGGTAGAGGAGAGTT	1	4.3478260869565215	No Hit
GATATGGAAGGCCGCGGGGAGGGGAAAAGAGGGGGGTATAATGAGGAATC	1	4.3478260869565215	No Hit
AGGGGGCAGGATATAGCGGGAGTTTAGATCAGCATCAAGAACTAACAGGG	1	4.3478260869565215	No Hit
GATCGGGAAAGGGGCGTGGAGGGGAAGAGGGGAGGGTATAGTGTAAAGCT	1	4.3478260869565215	No Hit
GATCGGGAGAGCGGCGGGGGGGGGAAGAGGGGAGGGGATAGAGTAGATTT	1	4.3478260869565215	No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAAAGGGGAGGGGGTTGAGAAGGGGT	1	4.3478260869565215	No Hit
CCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAG	1	4.3478260869565215	No Hit
GATTGTGAGAGGGGCGCGGAGGGGAAGAGGGGGGGCCTAAGGGTAGATTT	1	4.3478260869565215	No Hit
GAATGCAAAAGCGGTGGGTGGGGAAAAAAGGGAAGGGAAGGAGAAAGTAA	1	4.3478260869565215	No Hit
GATCGGGAGAGGGCCGGGTAGGGGAAAAGGGTAGGGGAAAGGGTAAAGAT	1	4.3478260869565215	No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA	1	4.3478260869565215	No Hit
GCTCCGGAGATCTTTTTTTTCGGGAAGGGTGTTGGCTTCACTGTATTTTC	1	4.3478260869565215	No Hit
GATAGGGAGGAAGGCGGGGGGGGGAAGAGAGGAAGAAAAAGGGGAAGGAT	1	4.3478260869565215	No Hit
GATCGGGAGAGGGGCGTGTAGGGGAAAAGGGGAGGGGATTAAGGAGAGTT	1	4.3478260869565215	No Hit
GAGGGGAGCAAAAGCAGGGGAGGAGAAAGCTGAGGGCGAAGCGGACTCAC	1	4.3478260869565215	No Hit
GATTGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGGGAAAGGGGTAGAAAT	1	4.3478260869565215	No Hit
GATCGGGAAAGGGGCGGGTAGGGGAAAAGGGGAGGGGATAGAGGAGAGTT	1	4.3478260869565215	No Hit
CGGGTTATTAGTAGAAACAAGGGCTTAACTTAAACAAGAAAAGAGGAAGA	1	4.3478260869565215	No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG	1	4.3478260869565215	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.0	0.0	0.0
2	0.0	0.0	0.0
3	0.0	0.0	0.0
4	0.0	0.0	0.0
5	0.0	0.0	0.0
6	0.0	0.0	0.0
7	0.0	0.0	0.0
8	0.0	0.0	0.0
9	0.0	0.0	0.0
10-14	0.0	0.0	0.0
15-19	0.0	0.0	0.0
20-24	0.0	0.0	0.0
25-29	0.0	0.0	0.0
30-34	0.0	0.0	0.0
35-39	0.0	0.0	0.0
40-44	0.0	0.0	0.0
45-49	0.0	0.0	0.0
50-54	0.0	0.0	0.0
55-59	0.0	0.0	0.0
60-64	0.0	0.0	0.0
65-69	0.0	0.0	0.0
70-74	0.0	0.0	0.0
75-79	0.0	0.0	0.0
80-84	0.0	0.0	0.0
85-89	0.0	0.0	0.0
90-94	0.0	0.0	0.0
95-99	0.0	0.0	0.0
100-104	3.4782608695652173	0.0	0.0
105-109	4.3478260869565215	0.0	0.0
110-114	4.3478260869565215	0.0	0.0
115-119	4.3478260869565215	0.0	0.0
120-124	4.3478260869565215	0.0	0.0
125-129	4.3478260869565215	0.0	0.0
130-134	4.3478260869565215	0.0	0.0
135-139	4.3478260869565215	0.0	0.0
140-144	4.3478260869565215	0.0	0.0
145-149	4.3478260869565215	0.0	0.0
150-154	4.3478260869565215	0.0	0.0
155-159	4.3478260869565215	0.0	0.0
160-164	4.3478260869565215	0.0	0.0
165-169	5.217391304347826	0.0	0.0
170-174	8.695652173913043	0.0	0.0
175-179	8.695652173913043	0.0	0.0
180-184	8.695652173913043	0.0	0.0
185-189	8.695652173913043	0.0	0.0
190-194	8.695652173913043	0.0	0.0
195-199	8.695652173913043	0.0	0.0
200-204	8.695652173913043	0.0	0.0
205-209	8.695652173913043	0.0	0.0
210-214	8.695652173913043	0.0	0.0
215-219	8.695652173913043	0.0	0.0
220-224	8.695652173913043	0.0	0.0
225-229	8.695652173913043	0.0	0.0
230-234	8.695652173913043	0.0	0.0
235-239	8.695652173913043	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE