FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated a8.34200000008777.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated a8.34200000008777.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length251
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTCGTCAATGGCATTCTGTGTGCTCTTCAGGTCGGCTGCATATCCT14.3478260869565215No Hit
GATCGGGAGAGGGGCGGGTAGGGGAAAAGGGGAGGGGATAGAGGAGAGTT14.3478260869565215No Hit
GATCGGGAGAAAGGCGGGGGGGGGAAGAGGGGAGGGTATTATGGAAAGCA14.3478260869565215No Hit
GATCGGGAGAGCGGCGTGGAGGGGAAGAGGGGGGGCGAAAGTGTAGATCT14.3478260869565215No Hit
GATCGGGAGAGGGGCGGGGAGGGGAAAAGGGGAAGGGGTAGAGGAGAGTT14.3478260869565215No Hit
GATATGGAAGGCCGCGGGGAGGGGAAAAGAGGGGGGTATAATGAGGAATC14.3478260869565215No Hit
AGGGGGCAGGATATAGCGGGAGTTTAGATCAGCATCAAGAACTAACAGGG14.3478260869565215No Hit
GATCGGGAAAGGGGCGTGGAGGGGAAGAGGGGAGGGTATAGTGTAAAGCT14.3478260869565215No Hit
GATCGGGAGAGCGGCGGGGGGGGGAAGAGGGGAGGGGATAGAGTAGATTT14.3478260869565215No Hit
GATCGGGAGAGCGGCGTGTAGGGGAAAAGGGGAGGGGGTTGAGAAGGGGT14.3478260869565215No Hit
CCCATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAG14.3478260869565215No Hit
GATTGTGAGAGGGGCGCGGAGGGGAAGAGGGGGGGCCTAAGGGTAGATTT14.3478260869565215No Hit
GAATGCAAAAGCGGTGGGTGGGGAAAAAAGGGAAGGGAAGGAGAAAGTAA14.3478260869565215No Hit
GATCGGGAGAGGGCCGGGTAGGGGAAAAGGGTAGGGGAAAGGGTAAAGAT14.3478260869565215No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA14.3478260869565215No Hit
GCTCCGGAGATCTTTTTTTTCGGGAAGGGTGTTGGCTTCACTGTATTTTC14.3478260869565215No Hit
GATAGGGAGGAAGGCGGGGGGGGGAAGAGAGGAAGAAAAAGGGGAAGGAT14.3478260869565215No Hit
GATCGGGAGAGGGGCGTGTAGGGGAAAAGGGGAGGGGATTAAGGAGAGTT14.3478260869565215No Hit
GAGGGGAGCAAAAGCAGGGGAGGAGAAAGCTGAGGGCGAAGCGGACTCAC14.3478260869565215No Hit
GATTGGAAGAGCGGCGGGGAGGGGAAGAGGGGAGGGAAAGGGGTAGAAAT14.3478260869565215No Hit
GATCGGGAAAGGGGCGGGTAGGGGAAAAGGGGAGGGGATAGAGGAGAGTT14.3478260869565215No Hit
CGGGTTATTAGTAGAAACAAGGGCTTAACTTAAACAAGAAAAGAGGAAGA14.3478260869565215No Hit
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG14.3478260869565215No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)