FastQCFastQC Report
Fri 24 Apr 2015
000000000-AE52Y l01n02 flu mrt-pcr updated a6.34200000008678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-AE52Y l01n02 flu mrt-pcr updated a6.34200000008678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517272
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGTTATTAGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTG113212.188597101718245No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAA103532.001461513478402No Hit
CGGGTTATTAGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGAC92181.782041169829413No Hit
GGGGGGAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG84021.6242905086685535No Hit
GGGGGGAGCGAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG62351.20536197590436No Hit
CGGGTTATTAGTAGAAACAAGGGTATTTTTCCTCAACTGTCATACTCCTC53281.030019022873846No Hit
GGGGGGAGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA51640.9983142331307321No Hit
CGGGTTATTAGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATT43400.8390169968604526No Hit
GGGGGGAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATG36980.7149043443294824No Hit
GGGGGGAGCGAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC27420.5300886187537698No Hit
CGGGTTATTAGTAGAAACAAGGGTGTTTTTCTCATGCTTCTGAAATCCTA27410.5298952968650923No Hit
GGGGGGAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT26860.5192625929878285No Hit
CGGGTTATTAGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAA26850.5190692710991509No Hit
GGGGGGAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGG25490.4927774942390077No Hit
CGGGTTATTAGTAGAAACAAGGAGTTTTTTGAACAAATTACTTGTCAATG22950.44367373451491676No Hit
GGGGGGAGCGAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA17230.33309361419137323No Hit
GGGGGGAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGA15030.290562798682318No Hit
GGGGGGAGCGAAAGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACA14280.2760636570315037No Hit
GGGGGGAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGC13300.2571181119411064No Hit
GGGGGGAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCT10110.19544842945297636No Hit
GGGGGGAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATA8740.16896333070415565No Hit
GGGGGGAGCGAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATA8420.1627770302664749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGGGC45350.0235.275639
TTATTAG54500.0233.986225
TATTAGT54550.0233.771776
GAGCGGC17350.0232.291069
ACGGGAG11300.0231.991153
ATTAGTA55850.0229.427057
GAGAGCG16900.0224.704157
GGGTTAT57400.0221.951222
AGAGCGG20200.0220.136128
TAGTAGA59450.0217.388579
GGGAGAG60250.0216.941915
GTTATTA59500.0213.911764
GGAGCGA36400.0213.701935
CGGGTTA59900.0213.505841
GAGCGAA36500.0213.452066
GGTTATT60000.0212.945833
GCGAAAG36950.0212.510138
CGAAAGC36850.0212.421989
GGAGAGG47350.0212.143626
ATCGGGA39950.0211.883622
Produced by FastQC (version 0.11.2)